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Studies assessing the reliability of the Borg rating of perceived exertion (RPE) scale for regulating exercise training intensity have reported mixed results. Differences in experimental designs and analytical techniques make it difficult to compare results. The purpose of this study was to examine the reliability of perceptually-based treadmill (TM) exercise. Eleven subjects (42.5 ± 19.5 yrs) completed a maximal test (GXT) and 3 RPEproduction trials (PT) on a TM. Each PT consisted of two 10-minute bouts where subjects selected speeds and grades to elicit an intensity equated with an RPE of 11-12 (RPE-L) and RPE of 15-16 (RPE-H). Speed and grade, heart rate (HR), oxygen uptake (V02), and ventilatory equivalent for oxygen (VE/V02) were assessed throughout the GXT and the last 3 minutes of each PT. Blood lactate concentration (BLC) was assessed at RPE-L and RPE-H during the GXT and at the completion of each PT intensity. Reliability was assessed using an ANOVA with repeated-measures, intraclass correlations, and absolute differences (ABS A) across the PTs at each intensity. No significant meangroup differences were noted in any of the physiological variables at either intensity across the PTs. Reliability coefficients were higher for the selected workload and the metabolic measures than for HR or BLC. Reliability increased across trials for all measures, and as a function of intensity for V02 (L = 0.91 & H = 0.98), HR (L = 0.60 & H = 0.84), and BLC (L = 0.50 & H = 0.64). Mean ABS A evaluated the inter-trial reliability for HR (bpm) and V02 (METS) at the low intensity PT 1 vs. PT2 = 013 bpm; A3.3 METS, PT 1 vs. PT3 = 010 bpm ; A3.3 METS, and PT2 vs. PT3 = A7 bpm; 01.6 METS and the high intensity PT 1 vs. PT2 = 011 bpm; A2.5 METS, PT 1 vs. PT3 = 011 bpm; A3.4 METS, and PT2 vs. PT3 = A9 bpm; A2.2 METS, respectively. Mean ABS A decreased for HR and V02 between PT2 vs. PT3. These data suggest that reliability of perceptually-based TMexercise varies among physiological measures and various analytical techniques, and individual variation in physiological data across PTs should be considered prior torecommending RPE for the self-regulation of exercise. / School of Physical Education