The FANTOM consortium has generated a large gene expression dataset of different cell lines and tissue cultures using the single-molecule sequencing technology of HeliscopeCAGE. This provides a unique opportunity to investigate novel associations between gene expression over time and different cell types. Here, we create a MatLab wrapper for a powerful and computationally intensive set of statistics known as Maximal Information Coefficient, and then calculate this statistic for a large, comprehensive dataset containing gene expression of a variety of differentiating tissues. We then distinguish between linear and nonlinear associations, and then create gene association networks. Following this analysis, we are then able to identify clusters of linear gene associations that then associate nonlinearly with other clusters of linearity, providing insight to much more complex connections between gene expression patterns than previously anticipated.

Extrakorporeal strålbehandling av skelettsarkom är en variant av strålbehandling där en del av en patients skelett opereras ut från kroppen. Skelettsegmentet transporteras sedan vidare till ett annat behandlingsrum där den bestrålas inuti ett fantom m.h.a. en linjäraccelerator. Detta sker medan patienten är nedsövd. Efter bestrålningen kan skelettsegmentet opereras tillbaka in till patienten. På Karolinska Universitetssjukhuset i Solna görs denna strålbehandling med en metod som kräver relativt lång bestrålningstid. Detta beror på fantomets kubiska form. Ju närmare ett fantom är strålkällan desto mindre stråltid behövs. Vid det kubiska fantomet används två strålfält, ett framför och ett bakom fantomet. Det här betyder att ifall fantomets position förs närmare strålkällan, så måste den föras tillbaka lika mycket åt andra hållet efter första strålfältet. Detta gör att det blir opraktiskt samt att man inte vinner någon tid. Målet med detta projekt var att skapa ett fantom som kan förflyttas så nära strålkällan som möjligt för att minska så mycket stråltid som möjligt. Detta kommer i sin tur minska den totala behandlingstiden. Genom att skapa ett roterande cylinderformat fantom som inte är riktningsberoende, så kunde fantomet förflyttas 25 cm närmare (från 95 cm till 70 cm), jämfört med det kubiska fantomets avstånd till strålkällan. Cylinderfantomet var gjord av akrylplast och en rotationsanordning konstruerades för att rotera fantomet. Vinkelhastigheten på rotationsanordningen sattes till 15 varv/minut. Det kubiska och cylindriska fantomet jämfördes genom simuleringar. Det visade sig att bägges stråldosfördelning var likvärdiga. Bestrålningstiden kunde förkortas ner från 640 sekunder till 340 sekunder utan att negativt påverka dosfördelningen jämfört med tidigare metod.

Submitted by Fernanda Rodrigues de Lima (fernanda.rlima@ufpe.br) on 2018-09-28T19:45:09Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) TESE Alexandra Carolina Gomes da Fonseca.pdf: 5722261 bytes, checksum: aac23d8838fdb0e78947a6d85d46b1fb (MD5) / Approved for entry into archive by Alice Araujo (alice.caraujo@ufpe.br) on 2018-11-20T22:57:58Z (GMT) No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) TESE Alexandra Carolina Gomes da Fonseca.pdf: 5722261 bytes, checksum: aac23d8838fdb0e78947a6d85d46b1fb (MD5) / Made available in DSpace on 2018-11-20T22:57:58Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) TESE Alexandra Carolina Gomes da Fonseca.pdf: 5722261 bytes, checksum: aac23d8838fdb0e78947a6d85d46b1fb (MD5) Previous issue date: 2017-07-18 / CAPES / A aplicação das duas tomografias teve como objetivo o estudo do comportamento do feixe de Raios – X em um fantom de solo tropical, após a filtração adicional e operando em intervalos de tensões entre 100 kV a 225kV. Para esse estudo foram utilizadas duas metodologias amplamente difundidas, que formaram uma metodologia única para a caracterização de materiais minerais do solo. Os equipamentos utilizados foram um micro - CT de Raios – X e um tomógrafo industrial de Raios Gama. Além da parte experimental, o estudo contou com uma fase simulada com os softwares XCOM e SpekCalC, essa fase foi de suma importância porque serviu de referência para as análises dos dados com as duas tomografias. As medições para avaliação da qualidade do feixe foram realizadas com software SpekCalC, as mesmas foram realizadas utilizando os mesmos parâmetros da fase de medidas do tomógrafo de Raios – X, os dados de saída analisados foram camada semi-redutora, energia média, energia efetiva e coeficiente de homogeneidade. Com o tomógrafo de Raios gama foi encontrado dado de intensidade relativa para os materiais puros, e a partir deles foram obtidos os valores de coeficiente de atenuação e densidade dos materiais. Com a técnica de atenuação de Raios – X foram realizadas medidas no fantom do solo e obtidos valores da atenuação de Hounsfield. A junção das metodologias mostrou que a boa resolução de densidade da tomografia de Raios gama foi de suma importância ao longo do estudo, porque indicou as melhorias da fase de construção do fantom, além de indicar os ajustes necessários para fase de medidas. Além da boa resolução temporal e espacial da tomografia de Raios – X, que detém um menor tempo de escaneamento e uma capacidade maior de detalhamento de materiais com dimensões muito pequenas ou particuladas (como os minerais utilizados) devido a sua resolução espacial. / The application of the two tomographies had the objective of studying the behavior of the X - ray beam in a tropical soil phantom, after additional filtration and operating in voltage ranges between 100 kV and 225 kV. For this study, two widely used methodologies were used which formed a unique methodology for the characterization of soil mineral materials. The equipment used was a micro - CT of X - rays and an industrial tomograph of gamma rays. In addition to the experimental part, the study had a simulated phase with the XCOM and SpekCalC softwares this phase was of great importance because it served as reference for the analysis of the data with the two tomographies. Measurements for beam quality evaluation were performed using SpekCalC software, which were performed using the same parameters as the X-ray tomograph measurements, the output data analyzed were semi-red, medium energy, effective energy and coefficient of homogeneity. With the gamma ray tomography, relative intensity data were obtained for the pure materials, and from them the values of the attenuation coefficient and density of the materials were obtained. With the X-ray attenuation technique, measurements were made on soil phantom and Hounsfield attenuation values were obtained. The combination of methodologies showed that the good resolution of gamma ray tomography was of paramount importance during the study because it indicated the improvements of the phantom construction phase, besides indicating the necessary adjustments for the measurement phase. In addition to the good temporal and spatial resolution of X - ray tomography, which has a shorter scanning time and a greater capacity for detailing materials with very small or particulate dimensions (such as the minerals used) due to their spatial resolution.

The topic of this thesis is architecture and settings of gothic horrors, particularly in films. The work explores the relationship between the genre of gothic horror and gothic architecture, mainly within examples of haunted castles. The aim of the work is to study in what way the haunted castles are depicted in films and how the gothic architecture is applied in their appearance. The thesis is divided in two parts. The first one is an analysis of some of the gothic horror tendencies which have an influence on the image of haunted castles. These can be observed in films as well as in literature and theatre since the beginnings of gothic horror in the second half of the 18th century until today. The second part uses these tendencies to analyse images of haunted castles in films Dracula (John Badham, 1979), Bram Stoker's Dracula (Francis Ford Coppola, 1992) and Van Helsing (Stephen Sommers, 2004).

Im Rahmen der Entwicklung der nächsten Version der FAnToM-Visualisierungssoftware wurde die bestehende Pipeline-Struktur durch Datenflussnetzwerke ersetzt. Dabei wurde die neue Datenstruktur 'Linienmenge' eingeführt. Da der Datenstruktur der Linienmengen bisher in der Literatur kaum Beachtung geschenkt wurde, sollen in dieser Arbeit sechs Algorithmen zum Erzeugen und Manipulieren solcher Linienmengen vorgestellt werden, welche auf die Datenstruktur angepasst oder speziell dafür neu entwickelt wurden. Dabei soll großer Wert auf die Effizienz der Algorithmen gelegt legen, da sich FAnToM unter anderem auch dadurch von anderen Visualisierungssystemen abhebt, dass es auch große Mengen an Daten von unstrukturierten Gittern schnell und effizient verarbeiten kann.

The task of this thesis is to study methods for the acquisition perfusní imaging based on dynamic MR imaging with T1 contrast. It describes methods of measurement of T1 relaxation time and the possibility of evaluating the results. It further describes the phantoms and their use. And it is here mentioned for the dynamic acquisition protocol perfusní imaging. There is also described in detail created a program for automatic control of the NMR system. In the experimental measurements are performed on static and dynamic phantom, are also evaluated perfusion parameters from the Flash sequence.

This paper deals with the effect of the MR pulse sequences on the temperature of the measured object. The theoretical part is dedicated to basic principle of magnetic resonance, the selected pulse sequences and risks that are connected with MRI. There is also described the draft of the testing phantom and information about the measurement and record of temperature during MR measurement. In the experimental part the effect of RF coils, pulse sequences and parameters of pulse sequences are tested on drafted phantom with experimental MR device located at the Institut of Scientific Instruments of the AS CR in Brno. These findings are then tested on live laboratory mouse. All results are analyzed and used as default data for developed simulation program.

In its theoretical part, this work deals with hospital information systems, their properties and development trends in the world, in Czech Republic and in the faculty Hospital in Plzeň in particular. A special reason for taking up this subject was the on-going transition to digital operation at the RTG departament of said hospital. Pictorial documentacion in digital form is presently becoming standard part of the medical do-cumentacion. The X-ray photographs can now be viewed at any client station of the hospital information system. As the existing stations were not originally intended for visualisation of pictorial medical documentacion, image quality tests have been carried out to determine the suitability of the station monitors for such operation. The main target of this work was to identify, using the technical tools available ath the RTG departament, a suitable image quality test, to verify its informative value and compare the existing hospital information system stations with dedicated diagnostic work station primarily intended for visualisation of medical X-ray documentation. The practical part of this work consisted of two tests using special graphic images and real patient`s X-ray picture including a specific diagnostic feature. A method of controlled interview was then used to establish the image quality and the degree of certainty with which that the doctors could identify the subject diagnostic feature. The test results were subject to statistical analysis using the linear regression analytic method whereby the informative value of the tests was determined. Finally, the operational properties of diagnostic work stations were compared to those of the hospital information system. In consideration of the small number of stations subjects to tests, the statistic analysis of this comparison was not carried out.

This master’s thesis deals with user influence on miniature antennas in mobile application. Electrically small antennas including fundamental performance properties and limitations are introduced in the theoretical section of this thesis. This section also deals with microstrip antennas and their common types. The accent is on PIFA antennas, their typical constructions and methods of miniaturization. In the practical section, dual-band PIFA antenna for operation at frequencies of systems GSM900 and GSM1800 is designed with the assistance of electromagnetic field simulator CST Microwave Studio. On this type of antenna, the user influence on impedance matching and radiation pattern in data mode (the user is typing message or browsing with the phone) is investigated. For this purpose the hand phantom, corresponding to author’s right hand, was made from agar based material. For typical position of human hand is designed matching network, which eliminate the user influence and ensure correct function of antenna.

This thesis deals with optimization of MRI measuring of phantoms containing the chicken embryo. The theoretical part of thesis is dedicated to the MRI fundamentals and description of pulse sequences. In the next part the options of keeping and monitoring the temperature are being discussed. The thesis also deals with design and realization of phantoms which are used for measuring. A browser of images captured by MRI was programmed in Matlab environment. The program allows users to look through the images with different settings of pulses sequences or kind of phantom. The final part of thesis describes the anatomical structures of chicken embryo and discussion of collected results.