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Uterine inflammation is one of the causes of a poor uterine environment. This can result in early embryonic loss in the mare due to an inhibition of or an increased secretion of prostaglandin F2α (PGF2α ). Oxytocin binds to endometrial cell receptors to activate prostaglandin synthesis. Increased secretion or accumulation of PGF2α within the uterus due to uterine inflammation can cause luteolysis and result in early embryonic loss. Supplementation with polyunsaturated fatty acids (PUFAs) has been shown to influence prostaglandin production in many species, although the effects on the mare remain unknown. Equine endometrial biopsies were collected and used to establish endometrial epithelial cell and explant cultures to determine the release of PGF2α and PGFM in response to oxytocin stimulation. Endometrial explant cultures were used to determine the inhibitory effects of Atosiban, an oxytocin receptor antagonist, and Indomethacin, a cyclooxygenase –2 inhibitor, on PGF2α secretion. Endometrial explant cultures were challenged with oxytocin (250 nM) and PGF2α concentrations were measured over time. The effects of PUFAs on equine endometrial prostaglandin production were determined using endometrial biopsies harvested on day two of behavioral estrus. Equine endometrial cells were established and shown to replicate in culture and on a basement membrane matrix. Equine endometrial explants stimulated with oxytocin had increased secretion of PGF2α and PGE2 and the secretion of PGF2α was inhibited through an oxytocin receptor antagonist and Cox inhibition. Endometrial explants stimulated with lipopolysaccharide had increased secretion of PGF2α and PGE2, however oxytocin stimulated to a greater extent than LPS. Supplementation with PUFAs, specifically DHA, decreased the secretion of PGF2α and PGE2, however AA and EPA failed to influence this response. Expression of mRNA was not influenced by fatty acid supplementation, however was altered by stimulus. Therefore DHA influences the inflammatory response in vitro through mechanisms other than enzyme expression. Decreased PGF2α production associated with PUFA supplementation in vivo, creates a likely approach for decreasing early embryonic loss associated with post breeding inflammation commonly seen in the equine industry.

In order to achieve a better understanding of the impact of exercise on the concentration of serum free fatty acids (FFA) during post-exercise recovery, the purposes of this study were: (1) to determine the relationships between exercise intensity, total exercise energy expenditure, and the concentration of serum FFA during post-exercise recovery; (2) to examine the effects of exoge- nous glucose on post-exercise serum FFA and hormones controlling the FFA response; and (3) to examine the impact of acute exercise on hunger. Untrained, 12-h fasted, college-age males performed cycle ergometer exercise at exercise intensities ranging from 29 to 59% peak ‘VO₂ for total energy expenditures ranging from 162 to 320 kcal. Blood samples, hunger ratings, and metabolic indices were collected or measured before, during, and for 3 h post-exercise. In response to exercise of approximately 300 kcal, FFA was elevated for 3 h post-exercise. The FFA response was a function of total exercise energy expenditure, rather than exercise intensity, or combined effects of these factors. The response was associated with low insulin concentration but no changes were observed in blood glucose, glucagon, growth hormone, or cortisol. Glucose ingestion and infusion studies demonstrated that possible mechanisms con- tributing to the post-exercise FFA response included decreases in FFA re-esterification, increases in triglyceride hydrolysis, and decreases in sympathetic input to adipose tissue. Exercise caused a suppression of hunger for 2 h post-exercise which was a function of the combined effects of exercise intensity and total energy expenditure. An increase in core temperature may have contributed to the anorexigenic effect of exercise. In conclusion, exercise, performed in and followed by a period of fasting caused an elevation of FFA for 3 h during post-exercise recovery. The post-exercise recovery period should be considered an important phase in the physiological impact of exercise on the storage and utilization of fat.

The objectives of this study were to investigate the different characteristics of the newly introduced breed, Waguli (Wagyu x Tuli) when comparing it with the Brahman breed. Twenty-four animals were used. Six steers of each breed were fed 94% concentrate diet (94C) and the other six were fed 86% concentrate diet (86C). Eight steers, two from each group, each were harvested at 128 days, 142 days, and 156 days on feed. Feedlot performance data indicated that Waguli steers were highly efficient (P < 0.05) and gained more than Brahman steers on a daily basis (P < 0.05). Carcass characteristic data showed that Waguli steers have larger ribeye area with more 12th rib fat thickness, marbling score and higher quality grade (P < 0.05). It is well known that Wagyu is a highly marbled and tender Japanese breed. It was found that the reason for the Waguli tenderness and low shear force values to be the low level of calpastatin activity (P < 0.05), the inhibitor of the postmortem proteolytic enzyme-calpain. While the toughness of the Brahman meat was due to the high level of calpastatin activity. The calpain activity did not differ between the two breeds. Shear force values agreed with the calpain and calpastatin activities data, in which the Waguli steaks showed less shear force values at day 7 and 10 postmortem than the Brahman steaks (P < 0.05). However, at day 14 postmortem there was no difference in shear force values between the two breeds (P < 0.05). Fatty acid data analysis indicated that Waguli steers had a profile with less saturated fatty acids (SFA) and more unsaturated fatty acids (UFA) content than those in Brahman steers fat. Looking at the ratio of unsaturated fatty acids (UFA) to saturated fatty acids (SFA), it is observed that Waguli steers had a greater ratio than Brahman steers. In conclusion, Waguli steers produce tender meat with good marbling ability, which likely to satisfy the consumers demand. In addition, they have a desirable average daily gain and feed efficiency with high polyunsaturated fatty acids comparatively with Brahman steers.

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