Thesis--Wisconsin. / Includes bibliographical references (leaves 106-107).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1969. / Typescript. Vita. Description based on print version record. Includes bibliographical references (leaves 97-99).

Thesis, PlanB (M.S.)--University of Wisconsin--Stout, 2004. / Includes bibliographical references.

Bacteria, belonging to the Lactobacillus bifidus group, were isolated from rectal swabs obtained from healthy babies receiving breast milk. The fermentation properties of these bacteria in cow's milk were studied. Strains potentially suitable for fermentation in a food product were selected according to their ability to produce lactic acid and no gas from lactose, as well as adhering to characteristics common to the bifidus group (Gram positive, catalase negative and blanching morphology). The five strains chosen for intensive study produced 1.14 to 1.25% lactic acid and less than 0.05% volatile acid. The latter organisms could be cultured readily and coagulated milk in less than 24 hr when using a 5% inoculum. Repeated subculture resulted in a change from the branched to the unbranched bacterial morphology. This transformation, which is not unusual among bifid bacteria, was accompanied by a change in ability to produce lactic acid, but no gas was produced by branched or unbranched strains. A great deal of variability in morphology, growth consistency, and acid production was observed among the more than 500 bacterial strains isolated. However, the bifid populations from Caucasian babies was not noticeably different from those of native Indian babies. All of the five strains selected utilized inulin, dextrin, mannitol, and melezitose. A 24 hr culture of fermented milk yielded a product with a mild pleasant natural flavour which could conceivably be altered, if desired, by adding sweeteners or flavouring agents / Land and Food Systems, Faculty of / Graduate

"Ititu" is a concentrated fermented milk utilized by Borana pastoralists in Southern Ethiopia. The effect of types of container used (glass, fibrous), smoking (smoked, non-smoked) and whey withdrawal (whey, non-whey withdrawn) treatments on the microbiological and chemical compositions of the fermented milks were investigated over a storage period of 28 days. Microbiological results indicated that the type of container used had significant effect (p $>$ 0.05) on total bacterial count (TBC) and lactic acid bacterial counts (LAB) for Weeks 1, 2, 3 and 4 and on coliform count (COLI) for Weeks 3 and 4. These counts determined for the fermented milks in the glass containers were found to be significantly lower compared to those in the fibrous vessels. There was significant difference (p $>$ 0.05) in the overall proximate composition for container and whey withdrawal treatments compared to smoking treatment. / An increased breakdown of the major caseins ($ alpha sb{ rm s1}$ and $ beta$) over the storage period was indicated. / A significant increase was noted on the content of the free amino acids compared to the total amino acids over the storage period.

No description available.

The genes coding for the expression of a ropy exopolysaccharide responsible for commercially desirable textural and rhealogical traits in fermented milk products by a natural lactococcal ropy isolate were sought. Using a transposon mutagenesis vector, pGh9:ISS1, three mutants lacking expression of the ropy exopolysaccharide were isolated. One of the mutants was chosen for further characterization. Using a Southern hybridization analysis, the interrupted gene was localized to the chromosome. The non-ropy mutant was further characterized and shown to be unable to produce ropy exopolysaccharide in fermented milk. A 2006 by fragment of the interrupted gene was sequenced. The DNA sequence over a short region showed homology to sugar transfer enzymes found in exopolysaccharide biosynthesis pathways. The DNA sequence was translated into its predicted amino acid sequences and two partial open reading frames of 236 and 338 amino acid residues in length were identified. These open reading frames were found to exhibit identity to glycosyltransferases present in exopolysaccharide biosynthesis pathways in other bacteria. / Graduation date: 1999

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No description available.