Infection of tall fescue (<i>Festuca arundinacea</> Schreb.) with the endophyte fungus (<i>Acremonium coenophialum</i>, Morgan-Jones and Gam) has been associated with toxicity symptoms observed in cattle. The overall objective was to investigate the influence of endophyte infection on growth and chemical composition of tall fescue and the toxicity of endophyte-infected (EI) tall fescue to cattle. In a greenhouse study with pairs of genetically identical EI and non-infected (NI) ‘Kenhy’ tall fescue clones, concentration of N, Ca, Mg, Al, B, Mn and Zn was higher and K and S was lower in NI, compared to EI tall fescue. Insect resistance was higher in EI, compared to NI. Yield and chemical composition of high and low EI tall fescue were measured at four growth stages (stockpiled, prebloom, bloom and regrowth after harvest at bloom), two sites (Glade Spring and Blackstone) and three rates of N fertilization (0, 40 and 80 kg/ha) in a field study. Tall fescue grown at Glade Spring was higher in N, Mg, Al, Cu, Fe and Mn, compared to Blackstone. Nitrogen fertilization increased N, Mg, Ca, B, Cu, Na, Zn and decreased NDF, ADF, cellulose, P and S concentration in tall fescue. Neutral detergent fiber, ADF, cellulose, lignin, Fe and Na were higher in low, compared to high EI tall fescue. Concentrations of Cu, Na and Zn in stockpiled and Ca, Cu, Na and Zn in bloom-cut tall fescue hay were below dietary requirements for 227-kg steers. A disc meter was also evaluated for use in predicting yield of tall fescue. The meter is useful for non-destructive estimation of yield. Three feeding studies were conducted with steers (6/treatment/year). Diets were orchardgrass/alfalfa hay, spring-cut EI tall fescue hay, spring-cut EI tall fescue silage and fall-cut EI tall fescue silage. Serum prolactin and cholesterol were depressed in steers fed fescue hay and silages, compared to steers fed orchardgrass/alfalfa hay. Differences in mineral composition of hay and silage were reflected in serum minerals in steers. Ergopeptine alkaloids in EI tall fescue may have contributed to the depression of serum prolactin. The spring-cut silage contained the highest concentration of ergopeptine alkaloids, compared to other diets. Steers fed the spring-cut tall fescue silage had the lowest basal and thyrotropin-releasing hormone stimulated prolactin compared to steers fed the other diets. / Ph. D.

Plant growth regulators (PGR's) including metsulfuron plus mefluidide at 10 plus 140 g ha⁻¹, chlorsulfuron plus mefluidide at 20 plus 140 g ha⁻¹, imazethapyr plus imazapyr at 67.5 plus 2.5, 96.4 plus 3.6, and 115.7 plus 4.3 g ha⁻¹, ACP 2100 at 60, 120, and 180 g ha⁻¹, and DPX L5300 plus mefluidide at 10 plus 140, 20 plus 140, and 70 plus 140 g ha⁻¹ were applied to ‘KY 31’ tall fescue (<i>Festuca arundinacea</i> Schreb.). All rates of imazethapyr plus imazapyr, ACP 2100, and chlorsulfuron plus mefluidide afforded a significantly higher turf quality than metsulfuron plus mefluidide. ACP 2100 at 120 and 180 g ha⁻¹, imazethapyr plus imazapyr, DPX L5300 plus mefluidide at 70 plus 140 g ha⁻¹, and metsulfuron plus mefluidide gave the most consistent seedhead suppression. When treating seven month old tall fescue, DPX L5300 plus mefluidide did not adequately suppress seedhead elongation. Metsulfuron plus mefluidide, regardless of timing, caused excessive injury. All rates of imazethapyr plus imazapyr and the upper rates of ACP 2100 afforded the best turf quality followed by chlorsulfuron plus mefluidide in 1988 to ‘Rebel’ and both years to ‘KY 31’. Red fescue (<i>Festuca rubra</i> L.) quality was best with chlorsulfuron plus mefluidide and the high rate of DPX L5300 plus mefluidide. All other treatments resulted in a poorer quality turf. For all field studies on all turf types, in general, multiple applications were not practical and often caused excessive injury regardless of timing. Root studies conducted in the greenhouse revealed that with one application, imazethapyr plus imazapyr, ACP 2100, and DPX L5300 plus mefluidide provided root dry weights ranging from 0.5 to 0.7 g, which was greater than metsulfuron plus mefluidide, chlorsulfuron plus mefluidide, and the mowed check which afforded root dry weights of 0.1, 0.3, and 0.2, respectively. With two applications ACP 2100 and DPX L5300 plus mefluidide afforded 350, 1100, 200 and 200% greater root volume than metsulfuron plus mefluidide and chlorsulfuron plus mefluidide and 200, 630, 600 and 600% greater root dry weights. Three applications are not recommended. In laboratory studies using ‘KY 31’ tall fescue, mefluidide enhanced the uptake of ¹⁴C-DPX L5300 after 48 hours by as much as 11% and the translocation of ¹⁴C by 8.4% to the young leaves, 9.3% to the old leaves and 6.1% to the culm. Radioactive material concentrated in the tips of leaves. No significant accumulation of ¹⁴C occurred in the crown or roots. / Ph. D.

Treatment of endophyte-infected tall fescue [Lolium arundinaceum (Schreb.) Darbysh] with the broad leaf herbicide Chaparral® can mitigate fescue toxicosis and enhance forage quality by suppressing seedhead emergence. Applying the herbicide to fescue pastures also reduces forage mass and promotes severe spot grazing when pastures are continuously grazed. A grazing experiment was conducted with steers (2013) and heifers (2014) to evaluate animal and plant responses in fescue-bluegrass (Poa pratensis L.) mixtures treated with Chaparral to determine the effects of grazing management on pasture carrying capacity, nutritive values, botanical composition, and animal performance. Continuous and rotational (i.e., four subdivisions to provide a 7-d grazing period and a 21-d rest period) grazing treatments were assigned to six, 3.0-ha fescue-bluegrass pastures in a randomized complete block design with three replications. Each pasture had six tester animals, and stocking rates were varied using put-and-take animals. Pastures were grazed from 16 April to 8 July 2013 (Year 1) and 20 May to 12 Aug (Year 2) and cattle were blocked by body weight for allotment to pastures. Pasture carrying capacities were greater (P = 0.07) for rotational compared to continuous pastures across both years. Calves on rotationally grazed pastures had greater average daily gain (P = 0.03) and gain per acre (P = 0.05) than those on the continuous treatment across both years. Canopies of continuously grazed pastures contained less (P = 0.01) tall fescue than those in rotationally grazed pastures. Herbage in pre-grazed paddocks had less NDF and ADF than post-grazed paddocks and continuously grazed pastures in 2013 (P < 0.10), but did not differ in 2014 (P > 0.10). In vitro digestible dry matter was greater (P = 0.07) in pre-graze rotational pastures in 2013 compared with continuous and post-graze rotational, and IVDDM did not differ (P < 0.01) between pre-graze rotational and continuous in 2014, but was greater than post-graze rotational. Crude protein was lower (P = 0.06) in both years in post-graze rotational pasture than in continuous or pre-graze rotational pasture, which did not differ. In 2013, there was no difference (P = 0.60) in root WSC among treatments; however, in 2014, WSC levels were greater (P = 0.01) in rotationally grazed pastures compared with continuously grazed pastures. This grazing experiment indicated that rotational grazing of Chaparral treated fescue-bluegrass mixtures can improve both animal performance and the sustainability of pasture productivity.

Master of Science / Department of Horticulture, Forestry, and Recreation Resources / Steven J. Keeley / Non-structural carbohydrates (NSC) are important for plant health and recovery from stress. Controlled-release N sources may moderate turfgrass vegetative growth, thereby maximizing NSC levels. Three studies were conducted to determine the effect of N source/timing on NSC levels, turfgrass visual quality, and color of ‘Midlawn’ bermudagrass and turf-type tall fescue. Additionally, the effect on low temperature tolerance of bermudagrass and brown patch incidence on tall fescue was investigated. Nitrogen sources included two polymer-coated ureas (PCU), a polymer-sulfur coated urea (SCU) and urea formaldehyde (UF). Total annual N was applied in either late summer or spring for bermudagrass, and either late summer or split between late summer and spring for tall fescue. Urea, applied at traditional timings, was a control in all studies. NSC status was determined at regular intervals by extracting two cores from each plot, defoliating, and measuring regrowth in a dark growth chamber. Turfgrass color, visual quality and brown patch incidence were rated monthly during the growing season. Bermudagrass low temperature tolerance was evaluated by subjecting plugs to a freezing regime and evaluating regrowth. Over the 2-yr study, N source did not have a significant effect on bermudagrass or tall fescue NSC levels, color, or visual quality. Timing of application, by contrast, did have a significant impact. For bermudagrass, August-applied N resulted in higher overall NSC levels and improved fall color. For tall fescue, split Sept/March applications improved color; but split Sept/ May applications reduced NSC compared to a single Sept application. Brown patch incidence was unaffected by N source or timing, though disease pressure was low. Timing of PCU application did not affect low temperature tolerance of bermudagrass, but PCU improved low temperature survival compared to urea.

The decrease in productivity caused by fescue toxicosis has been estimated to cost the United States livestock industry more than $1 billion per year due to reduced growth and diminished reproductive efficiency. This goal of the research presented in this dissertation is to enhance the knowledge base concerning the underlying physiological changes that occur during fescue toxicosis that lead to reduced intake and weight gain in cattle. As one of the factors associated with fescue toxicosis is a reduction in feed intake, achieving a consistent and adequate intake of toxins can be a complication. Results from experiment 1demonstrate that ruminal dosing of ground seed and a seed extract are able to mimic the classic symptoms of fescue toxicosis in cattle. This model whereby seed or extract is directly dosed into the rumen eliminates the possibility of reduced alkaloid intake due to refusal of feed by the animal. This model allows for more precise and repeatable dosing of alkaloids in fescue research. Experiment 2 results indicate that ingestion of endophyte-infected tall fescue leads to decreased fasting heat production in cattle. This is indicative of a reduction in maintenance energy requirements and may be related to a decrease in liver size or other metabolic activity in animals grazing endophyte-infected pastures. In addition, a reduction in basal metabolic rate may cause the compensatory gain often observed in cattle entering the feedlot after grazing endophyte-infected pastures. Data from experiment 3 provides evidence that whole body nitrogen and energy metabolism are not altered by fescue alkaloid ingestion. Experiment 3 also addresses the rate of feed degradation and ruminal passage rates in cattle ingesting endophyte infected fescue. While ruminal VFA profile is altered, this is likely due to reduced absorption, not increased production. The data from this experiment indicate that the reduction in weight gain and productivity seen during fescue toxicosis is primarily a function of reduced intake and not secondary effects of alkaloid ingestion.

Abstract can be viewed in PDF document. / Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 1994.

Photocopy of typescript. / Digitized by Kansas Correctional Industries

Consumption of endophyte-infected tall fescue results in a syndrome of negatively altered physiological systems, collectively known as fescue toxicosis. Another challenge to endophyte-infected tall fescue -based beef cattle operations is that the soils often are selenium (Se) poor, necessitating the need to provide supplemental Se. To test the general hypothesis that different forms of supplemental Se would ameliorate the negative effects of fescue toxicosis, predominately-Angus steers (BW = 183 ± 34 kg) were randomly selected from herds of fall-calving cows grazing an endophyte-infected tall fescue pasture and consuming vitamin-mineral mixes that contained 35 ppm Se as sodium selenite (ISe), SELPLEX (OSe), or an 1:1 blend of ISe and OSe (MIX). Steers were commonly weaned and depleted of Se for 98 d. Steers were assigned (n = 8 per treatment) to the same Se-form treatments upon which they were raised and subjected to summer-long common grazing of an endophyte-infected tall fescue pasture (0.51 ppm ergot alkaloids: ergovaline plus ergovalinine; 10.1 ha). Selenium treatments were administered by daily top-dressing 85 g of vitamin-mineral mix onto 0.23 kg soyhulls, using in-pasture Calan gates. The first project objective was to determine the effect of forms of supplemental Se on whole blood Se, serum prolactin, liver glutamine synthetase (GS) activity, carcass parameters, and growth performance (Experiment 1). In Experiment 1, whole blood Se increased for all treatments from day 0 to 22 and then did not change. Across periods, MIX and OSe steers had greater whole blood Se than ISe steer. Compared to ISe steers, MIX and OSe steers had more serum prolactin. Liver GS mRNA, protein content, and activity were greater in MIX and OSe steers than ISe steers. However, the ADG and carcass parameters were not affected by Se treatments. The second project objective was to determine the effect of forms of supplemental Se on serum clinical parameters of Experiment 1 steers (Experiment 2). In Experiment 2, across periods, MIX steers had more serum albumin than OSe, and ISe steers, respectively. Serum alkaline phosphatase (ALP) activity was greater in MIX and OSe steers. In addition, blood urea nitrogen (BUN), serum sodium, phosphorus, and magnesium concentration were affected by Se treatments. Partial correlation analysis revealed that serum albumin, BUN, and ALP activity were correlated with whole blood Se concentration. The third project objective was to evaluate the hepatic transcriptome profiles of Experiment 1 steers using microarray and targeted RT-PCR analyses (Experiment 3). In Experiment 3, bioinformatic analysis of microarray data indicated that hepatic glutamate/glutamine, proline, arginine, and citrulline metabolism was affected by different forms of supplemental Se. The mRNA expression of critical proteins involved in glutamate/glutamine (GLS2, GLUD1, GLUL), proline (PYCR1, ALDH18A1), and urea (ARG1, ARG2, OAT, NAGS, OTC, ORNT1) metabolism were differentially expressed by Se treatments. Collectively, we conclude that consumption of 3 mg Se/d as OSe or MIX forms of Se in vitamin-mineral mixes 1) increased whole blood Se content, an indicator of greater whole-body Se assimilation; 2) increased serum prolactin, albumin, and ALP, the reduction of which are hallmarks of fescue toxicosis; and 3) altered hepatic nitrogen metabolism, as indicated by changes in key enzymes of glutamate/glutamine, proline, and urea metabolism. However, 4) these positive effects on metabolic parameters were not accompanied by increased growth performance.

The first goal of the current research was to determine whether gene expression profiles differed between whole pituitaries of growing beef steers grazing pastures containing high (HE) or low (LE) amounts of toxic endophyte-infected tall fescue. The global (microarray analysis) and selected targeted (RT-PCR) mRNA expression patterns of pituitaries collected from beef steers (BW = 266 ± 15.5 kg) that had been randomly assigned to undergo summer-long grazing (89 to 105 d) of either HE (0.52 ppm ergot alkaloids) or LE (< 0.03 ppm ergot alkaloids) pastures were compared. Gene expression data were subjected to one-way ANOVA. The pituitaries of HE steers had 542 differentially expressed genes, and the pattern of altered gene expression was dependent on treatment. Targeted RT-PCR analysis corroborated these findings, including decreased expression of DRD2, PRL, POU1F1, GAL, and VIP and that of POMC and PCSK1, respectively. Canonical pathway analysis (Integrated Pathway Analysis, IPA) identified HE-dependent alteration in signaling of additional pituitary-derived hormones, including growth hormone and GnRH. In conclusion, consumption of endophyte-infected tall fescue alters the pituitary transcriptome profiles of steers in a manner consistent with their negatively affected physiological parameters. The second goal of this project was to test the hypothesis that sodium selenite (ISe), SEL-PLEX (OSe), vs. a 1:1 blend (MIX) of ISe and OSe in a basal vitamin-mineral (VM) mix would differentially alter pituitary transcriptome profiles in growing beef steers (BW = 183 ± 34 kg) commonly grazing an endophyte-infected tall fescue (HE) pasture. Steers were randomly selected from herds of fall-calving cows grazing HE pasture and consuming VM mixes that contained 35 ppm Se as either ISe, OSe, or MIX forms. Steers were weaned, depleted of Se for 98 d, and subjected to summer-long common grazing of a 10.1 ha HE pasture containing 0.51 ppm ergot alkaloids. Steers were assigned (n = 8) to the same Se-form treatments on which they were raised. Selenium treatments were administered by daily top-dressing 85 g of VM mix onto 0.23 kg soyhulls, using in-pasture Calan gates. Pituitaries were collected at slaughter and changes in global (microarray) and selected (RT-PCR) mRNA expression patterns determined. The effects of Se treatment on relative gene expression were subjected to one-way ANOVA. The form of Se affected the expression of 542 annotated genes. Integrated Pathway Analysis found a canonical pathway network between prolactin and POMC/ACTH/ α-MSH synthesis-related proteins, and that mitochondrial dysfunction was a top-affected canonical pathway. Targeted RT-PCR analysis found that the relative abundance of mRNA encoding prolactin and POMC/ACTH/ α-MSH synthesis-related proteins was affected by the form of Se, as were mitochondrial dysfunction-related proteins OSe steers appeared to have a greater prolactin synthesis capacity vs. ISe steers through decreased dopamine receptor D2 signaling, whereas MIX steers had a greater prolactin synthesis capacity and release potential by increasing TRH concentrations than ISe steers. OSe steers also had a greater ACTH and α-MSH synthesis potential than ISe steers. We conclude that form of Se in VM mixes affected genes responsible for prolactin and POMC/ACTH/α-MSH synthesis, and mitochondrial function in pituitaries of growing beef steers commonly grazing an HE pasture. The third goal was to test the hypothesis that sodium selenite (ISe), SEL-PLEX (OSe), vs. a 1:1 blend (MIX) of ISe and OSe in a basal vitamin-mineral (VM) mix would differentially alter selenoprotein profiles in pituitaries and livers of growing beef steers commonly grazing an endophyte-infected tall fescue (HE) pasture (i.e., the same steers used in Goal 2). The effects of Se treatment on relative gene expression were subjected to one-way ANOVA. The mRNA content of 6 selenoproteins in the pituitary was affected by Se treatments, along with two selenoprotein P receptors, whereas the expression of two selenoproteins was altered in the liver. We conclude that the change in selenoprotein gene expression in pituitaries indicates that OSe steers have a greater potential capacity to manage against oxidative damage, maintain cellular redox balance, and have a better quality control of protein-folding in their pituitaries than ISe steers. The change in selenoprotein gene expression by the liver indicates that MIX steers have a greater redox signaling capacity and capacity to manage oxidative damage than ISe steers.

The use of nitrogen (N) fertilizer is generally restricted on mixed species forage systems due to its stimulatory effect on grasses which increases competition with legume species. Reduced legume growth from this competition can compromise forage nutritive value and prospective yields. The controlled-release nature of several enhanced efficiency fertilizer N products holds the potential to improve legume persistence in mixed species pastures while providing supplemental N required by the grass component. The studies contained in this dissertation evaluated the effect of different enhanced efficiency N formulations (ATU, ESN, methylene urea, SuperU, and a 75% ESN: 25% urea blend) and untreated urea on yield, nutritive value, and legume persistence in a ‘Wrangler’ bermudagrass and ‘Durana’ white clover mixture (2014-2016 growing seasons), ‘KY-31’tall fescue and ‘Kenland’ red clover mixture (2015-2016 growing seasons), and ‘KY-31’ stockpiled tall fescue (2015-2017). The three studies were conducted at the University of Kentucky Spindletop Research Farm in Lexington, KY in a randomized complete block design. In the bermudagrass-white clover study, all enhanced efficiency N sources maintained white clover populations similar to the unfertilized grass/clover control, but only ESN caused greater clover composition than standard urea. Total forage yields increased linearly with N rate in all years, but dry weather conditions in the second and third years resulted in lower total yield. Forage nutritive value followed general trends throughout each growing season, but ESN’s ability to maintain clover resulted in higher nutritive value. In the tall fescue-red clover, total forage yields curvilinearly increased with N rate in 2015 but did not vary in 2016. ESN and ESN+urea blend treatments retained clover composition similar to that of the unfertilized control. Stockpiled forage yield increased with higher N rates. Enhanced efficiency N fertilizers with the ability to control N release can enhance forage yield while maintaining clover in mixed species swards.