The traditional filter paper assay for saccharifying cellulase originally described by M. Mandels et al (1976) has been modified to make possible low activity determinations of Trichoderma cellulases. The enzymatic activity appears to decline during a prolonged incubation period if no precautions have been taken. By means of adding bovine serum albumin and potassium chloride as protein stabilizers and sodium azide as an antimicrobial agent filter paper activities in the range from 0.02 to 0.37 (IUPAC assay, 1987) can be estimated by extending the incubation time up to 20 hours. Filter paper activity values obtained by this method may be compared to those obtained by the IUPAC assay by using a conversion factor from 1.4 to 1.7. Acid whey and sweet whey permeate have been investigated as media for growth and metabolite production by Trichoderma reesei QM 9414 using shake flask cultures and spore inocula. In the case of acid whey the mycelial growth after 2 weeks is 13 mg dry weight /ml substrate. The specific growth rate is 0.29/day. The fungus appears to metabolize the whey protein the first 2 weeks. The alkalinity of acid whey rises continuously over a three week period up to a pH of 8.5. In the case of whey permeate the maximal mycelial weight gain is 4.4 mg/ml which appears after 8 days. A rise in net soluble protein level comes after 3-5 days and reaches a maximum value of 0.23 mg/ml after 2 weeks. The pH of whey permeate rises continuously to 7.5 after 3 days and then slowly declines. The net production of cellulases is low on both media. Dilution 1:6 of the acid whey, supplementation with ammonium sulfate and pHadjustments did not enhance the production of cellulases. Acid whey supports a significant growth and sweet whey permeate shows potential for extracellular protein production. A literature review surveys the composition and uses of acid whey, environmental aspects of whey wastes, the fungus Trichoderma reesei, the mode of action of the Trichoderma reesei cellulase system and the structure of cellulose in cotton and wood. / Graduation date: 1994

The chemical and process industries rely largely on filtration processes to separate solid-liquid process / Thesis (M.Sc.Eng.)-University of KwaZulu-Natal, Durban, 2010.

Typescript (photocopy). / Digitized by Kansas Correctional Industries

Typescript (photocopy). / Digitized by Kansas Correctional Industries

The mechanism of how pulsatile flow affects flux behaviour in crossflow micro-filtration was investigated. The effects of pulsatile flow were sub-divided into shear effects and backflushing effects. A servo-valve hydraulic piston pump was applied to generate pulsatile flows in the membrane module with particular waveforms. Four types of fluid pulsation with specific flow-rate and pressure waveforms were produced for experimental tests. Two parameters, /dVcf\dt/ maxand Pmin, were examined independently for their effect during pulsatile flow, which was estimated by comparing the cake resistance during steady flow and pulsatile flow at the same mean crossflow velocity, trans-membrane pressure and membrane resistance. Filtration tests for all the pulsatile flows with clean water confirmed that pulsatility only affects cake depositions. Without particles, no flux improvement was obtained. The results for the microfiltration of 0.5g/1 silica suspension showed that for pulsatile flows without backflushing (i.e. no negative transmembrane pressure peak), the fluid pulsation decreased cake resistance when the shear related parameter /dVcf\dt/max exceeded a critical value for each given waveform. When the instantaneous transmembrane pressure reached negative values, i.e. back-flushing occurred, the cake resistance was reduced for all pressure waves tested. Cake resistance was reduced more for more negative P min. With two of the waveforms tested, the cake resistance was almost completely eliminated. In contrast, the shear affected cake resistance reduction differently for each waveform. Comparing cake reduction results for different pulsatile waveforms, it was found that, for the square wave, the cake resistance reduction was higher for both shear and backflushing effect tests, while for the short spike waveform, the cake resistance reduction was lower. The flux waveforms were seen to follow the variations in transmembrane pressure. The flux response time was longer than the time required for the pressure changes, but was not dependent on the direction of the pressure change.

Thesis (M.S.)--West Virginia University, 2003. / Title from document title page. Document formatted into pages; contains xii, 114 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 101-103).

Thesis (Ph. D.)--University of Texas at Austin, 2001. / Vita. Includes bibliographical references (leaves 301-310). Available also in a digital version from Dissertation Abstracts.

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