Made available in DSpace on 2016-09-27T13:40:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-08-21. Added 1 bitstream(s) on 2016-09-27T13:45:19Z : No. of bitstreams: 1 000870467.pdf: 1126912 bytes, checksum: 0d220e03b71df656b478827a129bd6d7 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / effluent to assess studies on cell mass production, yield, productivity, Chemical Oxygen Demand (COD) and economic viability of a project to produce bacterial biomass on an industrial scale. Initially, different concentrations of the substrate were performed with the purpose of increasing the productivity and the biomass recovery (¼, ½ and ¾ the initial volume) using microfiltration. Cultivations was accomplished in bioreactors (50 L), at 1,500 ± 200 lux, 32 ± 2°C during 5 days. The biomass was recovery by microfiltration then freeze-dried. Later, the biomass samples were analyzed regarding to proximate composition. Thus, an analysis was developed to assess the resources necessary to estimate costs and revenue that might occur during the project operating (10 years). The main viability indicators used were the Net Present Value (NPV), the Internal Rate of Return (IRR) and the payback. The results showed greater recovery and biomass productivity in bioreactor with higher organic matter (28.61 ± 0.71 g and 0.1144 g biomass L-1 day-1). The greater COD reduction was 69.23%. Except for the lipids and proteins, whose concentrations were higher in the biomass produced in the most concentrated substrates, the other components remained the same. Regarding the economic viability, the results showed that, with a productivity of 0.085 g biomass L-1 day-1, 9.58 tons of biomass could be produced during one year. The total investment for the project was estimated in R$ 6,361,456.51, with an annual costs of R$ 2,366,131.38 and incomes of R$ 3,870,913.15. Considering these results and the viability indicators of 23.70% for the IRR and R$ 4,466,192.26 for the NPV, per annum, the project was considered feasible, providing the recovery of the invested capital within 3.98 years of activity. / FAPESP: 2011/50274

Resumo:Rubrivivax gelatinosus, uma bactéria fotossintetizante com reconhecido potencial biotecnológico, foi cultivada em efluente para a realização de estudos sobre a produção de massa celular, rendimento, produtividade, Demanda Química de Oxigênio (DQO) e viabilidade econômica do processo em escala industrial. Inicialmente, diferentes concentrações do substrato foram realizadas com o objetivo de aumentar a produtividade e a recuperação da biomassa utilizando um sistema de microfiltração (¼, ½ e ¾ do volume inicial). Os cultivos anaeróbios foram realizados em biorreatores (50 L), com 1.500 ± 200 lux e 32 ± 2°C durante cinco dias. A biomassa foi recuperada por microfiltração, desidratada por liofilização e analisada para determinação da composição centesimal. O estudo da viabilidade econômica considerou recursos para estimar desembolsos e receitas previstos para ocorrer durante 10 anos. Os principais indicadores de viabilidade econômica analisados foram: o Valor Presente Líquido (VPL), a Taxa Interna de Retorno (TIR) e o payback. Os resultados indicaram maior recuperação de biomassa (28,61 ± 0,71 g) e produtividade (0,1144 g biomassa L-1 dia-1) no cultivo com maior quantidade de matéria orgânica. A maior redução de DQO foi de 69,23%. A composição centesimal revelou semelhanças entre os tratamentos, à exceção dos lipídeos e proteínas que foram maiores no cultivo mais concentrado. O total de biomassa produzida em um ano seria de 9,58 toneladas. O investimento total seria de R$ 6.361.456,51 a um custo operacional anual de R$ 2.366.131,38 e uma receita anual de R$ 3.870.913,15. Com esses valores, os indicadores TIR e VPL foram de 23,70% ao ano e R$ 4.466.192,26, respectivamente, o que demonstrou viabilidade do projeto, sendo possível recuperar o capital investido em até quatro anos de atividade industrial. / Abstract:effluent to assess studies on cell mass production, yield, productivity, Chemical Oxygen Demand (COD) and economic viability of a project to produce bacterial biomass on an industrial scale. Initially, different concentrations of the substrate were performed with the purpose of increasing the productivity and the biomass recovery (¼, ½ and ¾ the initial volume) using microfiltration. Cultivations was accomplished in bioreactors (50 L), at 1,500 ± 200 lux, 32 ± 2°C during 5 days. The biomass was recovery by microfiltration then freeze-dried. Later, the biomass samples were analyzed regarding to proximate composition. Thus, an analysis was developed to assess the resources necessary to estimate costs and revenue that might occur during the project operating (10 years). The main viability indicators used were the Net Present Value (NPV), the Internal Rate of Return (IRR) and the payback. The results showed greater recovery and biomass productivity in bioreactor with higher organic matter (28.61 ± 0.71 g and 0.1144 g biomass L-1 day-1). The greater COD reduction was 69.23%. Except for the lipids and proteins, whose concentrations were higher in the biomass produced in the most concentrated substrates, the other components remained the same. Regarding the economic viability, the results showed that, with a productivity of 0.085 g biomass L-1 day-1, 9.58 tons of biomass could be produced during one year. The total investment for the project was estimated in R$ 6,361,456.51, with an annual costs of R$ 2,366,131.38 and incomes of R$ 3,870,913.15. Considering these results and the viability indicators of 23.70% for the IRR and R$ 4,466,192.26 for the NPV, per annum, the project was considered feasible, providing the recovery of the invested capital within 3.98 years of activity. / Orientador:Elisa Helena Giglio Ponsano / Banca:Alex Akira Nakamura / Banca:Ricardo Borghesi / Banca:Márcia Marinho / Banca: Luzia Helena Queiroz / Doutor