Thesis (M.Arch.)--University of Hong Kong, 1999. / Includes special report study entitled : Circulation analysis. Includes bibliographical references. Also available in print.

Thesis (Ph. D.)--Ohio State University, 1985. / Includes vita. Includes bibliographical references (leaves 291-298). Available online via OhioLINK's ETD Center.

Thin-layer chromatography (TLC) is of great importance for the pharmaceutical industry as a simple, quick, and low cost analytical method. Considerable effort has been made over the past decades to combine the simplicity of TLC with the selectivity and sensitivity of mass spectrometry (MS) detection. In the pharmaceutical industry sensitivity is an especially important factor, since the allowed impurity level of most drugs is under 0.1%.The aim of the present thesis was to develop methods for the direct examination of pharmaceutical compounds from TLC plates by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI TOF MS). The study was started by comparing several approaches for the application of the matrix for direct TLC-MALDI including a newly developed electrospray matrix deposition method. This new method was found to be superior to the other techniques studied. It produced a stable signal, minimised analyte spreading, and hence allowed the scanning of a TLC plate to obtain chromatographic as well as mass spectral data. The plotted mass chromatograms assisted in spot location, and allowed the calculation of Rf-values. These showed good agreement with the Rf -values determined by UV detection. The decrease in mass resolution and mass accuracy commonly observed in TLC-MALDI TOF MS due to the uneven nature of the silica gel layer was corrected by internal recalibration on selected matrix ions during the scanning of the TLC plate. To enhance the signals recorded directly from a TLC plate the use of an extraction solvent prior the matrix application was explored. Further improvements in sensitivity were obtained by modifying a robotic x-y-z axis motion system to act as an electrospray deposition device and by use of special Si 60 F[254] HPTLC-MALDI targets. Using both approaches sensitivities in the high fmol range were obtained. To minimise matrix interference, which can suppress analyte signals, the application of suspensions of particles of different materials and sizes (Co-UFP, TiN, TiO[2], graphite and silicon) onto eluted TLC plates were investigated. The structural analysis of pharmaceutical compounds was achieved by post-source decay - matrix-assisted laser desorption/ionisation (PSD-MALDI) mass spectrometry performed directly on the separated spots. TLC-MALDI MS is not only applicable to the qualitative analysis of pharmaceutical compounds. The generation of quantitative data by using a structural analogue as an internal standard is also described. Different approaches to the incorporation of the internal standard into the TLC plate were tested. The most successful approach was to develop the TLC plate in the mobile phase to which the internal standard was added. Good accuracy, precision, linearity and sensitivity was obtained using this approach.

No description available.

This thesis describes the design of a data structure for use with Digitised Terrain Elevation Data (DTED) in Terrain Reference Navigation (TRN) systems. The data structure is based on a variant of quad-tree and oct-tree data structures to provide an efficient representation of terrain in terms of storage requirements and acccss operations. These data structure are applied to flight path planning operations in mission management applications. The algorithms developed for flight path planning have becri implemented in the C programming language for a standard PC. Current research in TRN systems is reviewed and attention is given to the use of hierarchical data structures to cope with the potentially large data base needed for DTED files. Data structure combining quad-trees and oct-trees are developed with an emphasis on data reduction using pointerless trees and the use of locational codes to provide straightforward mapping between quad-trees and oct-trees, in other words, between two-dimensional co-ordinates and three-dimensional co-ordinates. Analysis of these algorithms is described for two DTED files to illustrate storage improvements and to verify a set of database access operations. These data structures are applied to problems of flight path planning where the navigation space comprises objects above a specific altitude and this three-dimensional space is searched for a flight path which avoids the obstacles and satisfies specific operational criteria. Algorithms are developed to extract a visibility graph from the terrain database and to determine the preferred flight path from a set of paths which satisfy defined constraints. Several search techniques are developed which exploit the efficiency of the quad-tree and oct-tree data structures. These methods are extended to real-time flight-path planning where predicted times for access operations are used to direct flight path extraction by varying the tree resolution during computation of the flight path. A comprehensive set of results are provided to illustrate: the storage efficiency of quad-tree and oct-tree data structures the application of pyramid structures to represent navigation space analysis of the time to compute the visibility graph and to extract flight paths integration of these methods with a real-time mission management simulation on a PC The thesis draws conclusions on the efficiency of these techniques for the represcntation of DTEDs and to access objects in TRN systems. It is observed that the use of hierarchical data structures in the form of quad-trees and oct-trees offers significant improvement in accessing DTEDS, for future use in TRN systems. The thesis concludes by outlining areas of further work where the techniques can be further &N, cloped for applications in mission management and navigation using DTED files.

No description available.

Pterosaurs represent the earliest appearance of only three clades of flying vertebrates, the pioneers of aerial vertebrate ecospace, and the lineage to produce the largest known flying organisms. The origins of the pterosaurian flight apparatus have been difficult to ascertain, in part, due to incomplete or two-dimensional preservation of the earliest (Triassic—Jurassic) pterosaur remains. An exceptional early pterosaur specimen that is preserved in three dimensions, the holotype and only known specimen of Arcticodactylus cromptonellus (Fleming Fjord Formation, Greenland) may help address these problems. However, it has remained mostly encased within matrix to protect the delicate elements, obscuring external study. Here I present new synchrotron tomographic scan data of the forelimb (wing-forming) elements of Arcticodactylus cromptonellus. I find that the forelimb of Arcticodactylus is a structural intermediate between the forelimb of early archosaurs and derived pterosaurs. In light of this intermediacy, I reexamined the phylogeny of early Pterosauromorpha, completely reviewing forelimb characters with additional consideration given to other important anatomical regions for pterosauromorph phylogeny. I find that the contents of Lagerpetidae represent a grade of non-pterosaur pterosauromorphs and that the pterosauromorph Scleromochlus taylori is actually closely-related to crocodylomorphs. I recover Arcticodactylus as the earliest-diverging pterosaur, with the pterosaurs of the early Mesozoic (Triassic—Early Jurassic) forming a highly-nested, gradational relationship around a monophyletic Late Mesozoic pterosaur clade with very few multispecific groups exclusive of this latter clade. The sum of this work is an understanding of the current pterosaur fossil record as preserving the gradual assembly of the pterosaur bauplan in exquisite detail. / Master of Science / Flight has only evolved three times within animals with backbones. The first of these three is a group of distant relatives of birds called pterosaurs, which evolved flight independently from birds and produced the largest ever flying animals. Flight requires a suite of specialized adaptations, and these can obscure our understanding of how flying animals evolved if all we have are poorly-preserved fossils or only specialized flighted forms. These problems can be found in the origins of pterosaur flight, with well-preserved (non-crushed or deformed) skeletons generally known only from very late forms. An exception to this rule is the early pterosaur Arcticodactylus cromptonellus, represented by three-dimensionally-preserved skeletal remains. Using CT imagery to study the skeletal anatomy obscured by rock, I describe the forelimb anatomy of Arcticodactylus cromptonellus and include it within an analysis of the evolutionary relationships of pterosaurs and other reptiles. Arcticodactylus is found to be the earliest-originating form of pterosaur known, and it possesses a mixture of features found in non-pterosaurs and in pterosaurs. Arcticodactylus demonstrates the gradual acquisition and timing of the assembly of pterosaur flight anatomy.

No description available.

Shading and texturing are two techniques that flight simulators can take advantage of to increase scene realism. Shading imitates the effects of light reflecting from a surface. Texture refers to superimposing a pattern on a surface to give the illusion of extra detail and realism. In this report, several techniques for shading and texturing are evaluated with respect to their applicability to visual flight simulators. The image quality produced by shading and texturing is a function of computation cost. The Phong model is found to produce the most realistic shading, but is too computationally expensive. The Gouraud shading model improves upon the Phong model in that realistic shading is produced with less computational effort. The table look up technique was found to be the most flexible and realistic way to produce texture on the surface of a polygon. It is shown that true perspective shading is cost effective when texture is required because the hardware needed to produce texturing and true perspective shading are very similar.

No description available.