Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-01-27T13:32:41Z No. of bitstreams: 1 RenataKalineSouzaEstevam_TESE.pdf: 2591439 bytes, checksum: 8a5092689ed7b6122d0702466b33f854 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-01-31T13:43:08Z (GMT) No. of bitstreams: 1 RenataKalineSouzaEstevam_TESE.pdf: 2591439 bytes, checksum: 8a5092689ed7b6122d0702466b33f854 (MD5) / Made available in DSpace on 2017-01-31T13:43:08Z (GMT). No. of bitstreams: 1 RenataKalineSouzaEstevam_TESE.pdf: 2591439 bytes, checksum: 8a5092689ed7b6122d0702466b33f854 (MD5) Previous issue date: 2016-05-27 / A flora??o ? um processo vital durante o ciclo de vida das plantas e ? marcado pela convers?o do meristema apical vegetativo em reprodutivo devido a intera??es de fatores internos e externos ? planta. Apesar de amplo conhecimento ter sido gerado nessa ?rea, ainda se conhece muito pouco sobre o processo de flora??o e frutifica??o em tomateiro. Pesquisas anteriores realizadas pelo nosso grupo identificaram o cDNA hom?logo a Prote?na Reprimida por Auxina (ARP) em bibliotecas subtrativas reprodutivas de tomateiro. Existem poucos dados sobre a prote?na ARP na literatura, h? relatos de que o gene ARP est? relacionado com a matura??o do fruto em morango, dorm?ncia da gema lateral em ervilha e matura??o do p?len em tabaco, mas a fun??o da prote?na ARP ainda n?o est? clara. Portanto, o intuito deste trabalho foi compreender o papel da prote?na ARP no desenvolvimento vegetativo e nos processos de flora??o e frutifica??o por meio da perda e/ou do ganho de fun??o deste cDNA em plantas transg?nicas de tomateiro contendo o cassete de superexpress?o em orienta??o senso e antissenso para este cDNA. Dessa forma, foram observadas algumas altera??es fenot?picas e estruturais nas plantas transg?nicas (35S::SlARP antissenso e senso), como flora??o e frutifica??o precoce verificada nas plantas 35S::SlARP antissenso nas gera??es T1 a T4, em rela??o ?s controles (transformada com o plasm?deo vazio e n?o transformada). Na an?lise histol?gica, notaram-se ?vulos maduros nas flores das plantas 35S::SlARP antissenso (gera??es T2 a T4), enquanto que as flores das controles n?o apresentaram ?vulos maduros no mesmo per?odo de tempo. Outra modifica??o observada foi o n?mero superior de gemas laterais desenvolvidas nas plantas 35S::SlARP antissenso nas gera??es T3 e T4 quando comparado ?s plantas 35S::SlARP senso e ?s plantas controles (transformada com o plasm?deo vazio e n?o transformada) . Essas produziram ramos com folhas, flores e frutos. Assim como altera??es estruturais nos pec?olos das plantas 35S::SlARP antissenso, incluindo uma aparente quantidade maior de elementos de vaso (xilema e floema) do que os pec?olos as plantas 35S::SlARP senso e mutantes relacionados ? sinaliza??o da auxina (dgt e entire) e plantas controles. Portanto, estes dados sugerem que a prote?na ARP possa estar envolvida na flora??o, frutifica??o e na dorm?ncia das gemas axilares em tomateiro. / Flowering is a vital process during the plant life cycle and it is characterized by the conversion from the vegetative apical meristem into reproductive meristem due to internal and external factors. Despite the considerable knowledge has been produced in this field, not much is known about the flowering and fruiting process in tomato. Furthermore, previous research from our group has identified a cDNA with homology to AUXIN REPRESSED PROTEIN (ARP) in reproductive subtractive libraries from tomato. In the literature, there are few reports where the ARP gene has been associated to fruit ripening in strawberry, dormancy in pea and pollen maturation in tobacco plants, but it is not clear the ARP protein function yet. Therefore, the aim of this work was to understand the role of ARP protein in vegetative development, flowering and fruit set processes through the loss and/or gain of function using this cDNA in transgenic tomato plants with the overexpression cassette constructs in sense and antisense cDNA orientation. Thus, it was observed some phenotypic and structural modifications in transgenic plants (35S::SlARP antisense and sense) such as early flowering and fruiting that was observed in 35S::SlARP antisense plants from the T1 to T4 progeny when compared to controls plants (with the empty vector and wild type plant). In the histological analysis, it was observed mature ovule in 35S::SlARP antisense flowers (progeny T2 to T4), while for the controls plant it wasn?t observed a mature ovule at the same period of time. The other modification observed was a higher number of lateral buds developed in 35S::SlARP antisense plants (progeny T3 and T4) when compared to 35S::SlARP sense and controls plants. These plants produced branches with leaves, flowers and fruits. Besides, it has been observed some structural changes in the petioles from 35S::SlARP antisense plants, including a high number of vessel elements (xylem and phloem) when compared to 35S::SlARP sense, and to dgt and entire mutants (related to auxin signaling) and controls plants (with empty vector and wild type plant). Therefore, these data suggest that ARP protein may be involved in flowering, fruit set and dormancy of axillary buds in tomato.