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published_or_final_version / Obstetrics and Gynaecology / Master / Master of Philosophy

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A new cryptand, 1,18-bis[(phenylmethoxy)methyl]-3,16,20,23,26,29,32,35-octaoxatetracyclo-[16.9.9,05,10 09,14]hexatriaconta-5,7,9,11,13-pentaene was synthesized from cis-9,19-bis(benzyloxymethyl)-1,4,7,11,14,17-hexaoxacycloeicosane-9,l9-diyldimethanol and l,5-bis(bromomethyl)naphthlene in the presence of t-butoxide base. The overall syntheses involved five steps. The new cryptand compound has a melting point at 164-1650 C, and the yield was 18.5%. The NMR spectra and the crystal structure determination proved the synthesized cryptand the same as that desinged.Fluorescent measurements were performed with this cryptand. The fluorescence was found to be quenched by cesium ion, and the quenching increased with the increasing of cesium ion concentration. The average quenching of the fully complexed (with Cs +) cryptand was estimated as 20 + 2% by a computerized data fitting program. The complexation constant was determined to be 16 ± 5 M-1. A possible explanation for these observations is presented. / Department of Chemistry

The object of this thesis is to present two novel applications of Spatiotemporal Image Correlation Spectroscopy (STICS) to biological systems. STICS is a technique which uses the correlations in pixel intensity fluctuations of an image time series, captured under fluorescence microscopy, to measure the speed and direction of a flowing population of fluorescently labeled molecules. The method was first applied to measure the dynamics of transport vesicles inside growing pollen tubes of lily flowers. The measured vector maps allowed to confirm the presence of actin filaments along the periphery of the tubes, as well as the presence of a reverse-fountain pattern in the apical region. In a second set of experiments, STICS was used to measure the retrograde flow of filamentous actin in migrating chick DRG neuronal growth cones. These results serve as proof of principle that STICS can be used to probe the response of the growth cone cytoskeleton to external chemical cues.

Thesis (Ph. D.)--University of Oregon, 2007. / Typescript. Includes vita and abstract. Includes bibliographical references (leaves 176-185). Also available for download via the World Wide Web; free to University of Oregon users.

Thesis (Ph.D.). / Written for the Dept. of Chemistry. Title from title page of PDF (viewed 2008/01/12). Includes bibliographical references.

Thesis (Ph. D.)-- State University of New York at Binghamton, Department of Chemistry, 2009.

Thesis (Ph. D.)--Florida State University, 2004. / Advisor: Dr. Alan G. Marshall, Florida State University, College of Arts and Sciences, Dept. of Chemistry and Biochemistry. Title and description from dissertation home page (viewed Jan. 12, 2005). Includes bibliographical references.