The Unsettled Object is an installational art project that considers the instability of objects in regards to their assembly, classification, and presentation, underpinned by the context of the museum and supported by Michel Foucault’s notion of the classificatory grids he discusses in The Order of Things: an archaeology of the Human Sciences. (Foucault,1970) The artefacts are being fabricated as a response to the corporeal body-on-display; its surfaces, spaces and volumes. An underlying notion of temporality and mutability is indicated in the processes of making, the objects, material responsiveness and the devices employed in the presentation of the work.

People with diabetes suffer from an increased incidence of atherosclerosis, possibly due to the hyperglycaemia associated with this disease. Glucose may covalently modify proteins via glycation and glycoxidation reactions. Reactive aldehydes (e.g. methylglyoxal and glycolaldehyde) generated from these glycation and glycoxidation reactions, lipid peroxidation and other metabolic pathways may also modify proteins in glycation and glycoxidation reactions. These reactions can result in the formation of advanced glycation end-products, which are increased in diabetes and associated complications such as atherosclerosis. Low-density lipoproteins (LDLs) are the main source of lipid in atherosclerotic plaques, and the lipid-laden foam cells contained within. Modification of the single protein in LDL, apolipoprotein B-100 (apo B) by glucose and aldehydes may result in recognition of these altered LDL particles by macrophage scavenger receptors and cellular accumulation of cholesteryl esters; such accumulation is characteristic of atherosclerotic foam cells. The extent and nature of the modifications of LDLs that give rise to this behaviour have been poorly characterised, especially in regards to modification/oxidation of protein versus lipid components induced by glucose and low-molecular-mass aldehydes. Therefore the aims of this project were to: 1) characterise LDL modification by glucose, methylglyoxal and glycolaldehyde; 2) examine the effect of these modified LDLs on arterial cells by monitoring cellular viability, proliferation and cholesterol and cholesteryl ester levels; and 3) examine macrophage handling of apo B from these modified LDLs. Glycolaldehyde induced more rapid and more extensive changes to LDL than methylglyoxal, which was significantly more modified than LDL exposed to glucose, in the presence or absence of Cu2+. LDL was modified by glycolaldehyde and methylglyoxal in a time- and concentration-dependent manner. These aldehyde-modified LDLs were significantly more negatively charged relative (determined by changes in relative electrophoretic mobility), more aggregated (by SDS-PAGE) and lost more Arg, Lys and Trp residues (assessed by fluorescence-based assays) than glucose-modified and control LDLs. Glucose-modified LDL had more modest increases in net negative charge, aggregation and only significantly lost Arg residues. Under the conditions examined none of the modified LDLs contained significant levels of the protein oxidation products DOPA and o-tyrosine, the lipid oxidation products 7-ketocholesterol and cholesteryl ester hydro(pero)oxides, nor marked depletion of the major antioxidant α-tocopherol or significant radical formation (EPR spectroscopy). Therefore these LDLs were glycated, but not (glyc)oxidised, and so allowed the cellular uptake of glycated LDL, rather than glycoxidised LDL, to be examined. These glycated LDLs had no effect on the cellular viability (assessed by LDH release), cell protein (BCA assay), and cholesterol and cholesteryl ester levels (quantified by reverse-phase HPLC) of endothelial and smooth muscle cells. The glycated LDLs also had no effects on human and mouse macrophage viability, protein and free cholesterol levels. However, exposure of macrophages to some of the glycated LDLs resulted in significant accumulation of cholesteryl esters and apo B. The greatest cellular accumulation of cholesteryl esters was in cells exposed to glycolaldehyde-modified LDL, which occurred in a time- and concentration-dependent manner. Less cholesteryl ester accumulation was observed in cells exposed to methylglyoxal-modified LDL, but some conditions resulted in significantly more cellular cholesteryl esters as compared to control LDLs, unlike glucose-modified LDL. Macrophages endocytosed significantly more apo B from glycolaldehyde-modified LDL labelled with 125I on the apo B, than methylglyoxal-modified 125I-LDL. Apo B from methylglyoxal-modified 125I-LDL was also endocytosed and degraded in greater amounts than control 125I-LDLs, unlike glucose-modified 125I-LDLs. The glycation of LDL by some low-molecular-mass aldehydes have been shown to result in model foam cell formation as characterised by cholesteryl ester and apo B accumulation. This accumulation correlated with increases in net negative charge, aggregation and loss of Lys and Trp residues of the apo B in glycated LDL particles. However, the differences in cellular uptake of glycolaldehyde- versus methylglyoxal-modified LDL were not completely resolved and it is postulated that this may arise from the extent or type of products formed on key amino acid residues, resulting in differential uptake by macrophage scavenger receptors, rather than loss of particular amino acids per se. Therefore these studies provide a potential mechanism to explain the increased atherosclerosis in people with diabetes, and a suitable model to examine the potential inhibition of the effects of glycated LDLs. This could provide potential therapeutic interventions to reduce diabetes-induced atherosclerosis.

The company Kiviks Musteri AB produces a pear base wine that forms stable foam, which is problematic from a production perspective. The aim of this thesis was to investigate the factors underlying foam stability in the pear base wine and to find means for its reduction. This was done by foam testing wines and varying several variables, such as the fining agents normally used in the wine production (bentonite, gelatin, siliceous earth and activated carbon), enzyme treatment, and by changing the fermenting yeast species. Results: The wine started to form stable foam during fermentation, and foam stability could be reduced by using more bentonite and carbon during the fining process. The other fining agents appeared to have only limited impact on foaming characteristics. No pectin was present according to the pectin test, but protein bands were evident from SDS PAGE analysis, though absent in samples treated with increased doses of bentonite. In conclusion, pectin is not a major foaming agent in the wine, the yeast is most likely the producer of the foaming agents, carbon and bentonite have a reducing effect on foam stability, bentonite also reduces protein content. Proteins are likely to be involved in the foam stabilization but are not the sole contributors to stable foam.

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CO₂ enhanced oil recovery (CO₂-EOR) in residual oil zones has emerged as a viable technique to maximize both the oil production and carbon storage. Most CO₂ field projects suffer from inadequate sweep because of high mobility of CO₂ compared to the oil. Gas conformance techniques have the potential to further improve the effectiveness of CO₂-EOR projects. The choice of mobility control to improve the sweep efficiency is critical and simulation studies with hysteretic relative permeability and mechanistic foam model can assist in the choice of technique and optimization of the process for each reservoir. Two promising mobility control practices of Water Alternating Gas (WAG) and foam are evaluated using the in-house compositional gas reservoir simulator (DOE-CO₂). The effect of hysteresis and cycle dependent relative permeability on WAG and foam injections incorporating a new three-phase hysteresis model has been investigated. Simulations are performed with and without hysteresis to assess the impact of the saturation history and saturation path on gas entrapment, fluid injectivity and oil recovery. The foam assisted technique in CO₂-EOR processes has also been investigated. Here foam is generated in-situ by injecting surfactant solution with CO₂ rather than directly injecting foam. A simplified yet mechanistic population-balance model implemented in the in-house simulator has been applied to test the impact of foam. The results have been compared with an empirical foam model which is the standard model in commercial simulators. Simulations have been performed on actual field models for selection and optimization of the CO₂ injection scheme, quantifying the impact of hysteresis, depicting the effectiveness of CO₂-EOR process as against a surfactant flood, the effectiveness of foam assisted floods and insights into low tension gas flooding process. All the above analyses have also been performed on layer cake models with properties replicating the Permian Basin carbonate reservoirs and Gulf Coast sandstone reservoirs. Hysteresis shows an improvement in oil recovery of gas injection schemes where flow reversal takes place. Foam has been found to be effective and the models show lower CO₂ utilizations factors compared to the case without foam. / text

The production of highly aerated foods remains a challenge that requires skill to obtain desired appearance and texture. Since foams are fragile and inherently unstable, evaluation of structure changes with time requires a delicate approach. Non-invasive but informative evaluation of changes in aerated food properties is a desired goal to be achieved in this thesis. I studied the aging of egg white foams of different void fraction using two noninvasive techniques - ultrasonic spectroscopy and electrical resistivity - with a view to understanding aging mechanisms that would affect the quality of the final product. To help in interpretation of the results, a commercial high void fraction foam, Gillette (Sensitive), was also analyzed. As a support technique for ultrasound results I used image analysis in order quantitatively evaluate the size of bubbles in the foams. Large increases in attenuation were observed with increasing aging time and frequency. Results were modeled using an effective medium theory originated by Foldy (1945) in order to understand the factors governing foam lifetime and texture. From electrical resistance measurements, liquid fraction changes in an egg white foam column were evaluated quantitatively and qualitatively by using a generalized free drainage model. Although egg-white foams were more prone to changes due to drainage, I observed that for all foams the ultrasonic scaling parameter alfa*lambda/freq was proportional to the square of average bubble size (indicative of a diffusively driven aging process due to disproportionation of bubbles). Slopes of alfa*lambda/freq versus aging time were 6*10-8 and 11*10-8 for egg white foams of void fraction 0.65 and 0.78, respectively, indicating that disproportionation progressed approximately twice as fast in the high void fraction foam. The slopes of alfa*lambda/freq versus aging time were similar for both Gillette foam void fractions (0.93 and 0.91) at a value of 1.5*10–8 s-1, attributable to a lower solubility of isobutane compared to air. By combining ultrasound and electrical resistivity, this thesis has provided novel insights into understanding instability processes occurring in foams. Potentially, ultrasound techniques could be used instead of imaging for foam aging studies, since non-invasive and non-destructive measurements of attenuation and phase velocity permit interrogation of opaque foam structures.

This thesis presents the development of a novel robotically effected plastic foam sculpting system for rapid prototyping and manufacturing purposes. The developed system is capable of rapidly sculpting physical objects out of expanded and extruded polystyrene using an electrically heated Nichrome sculpting tool. An overview of current conventional rapid prototyping systems indicated that the main disadvantages lie in the limited size of objects which can be built, the relatively long time involved to produce one part and the high cost of the systems and materials. An extensive literature and technology review was conducted on work which was similar to the novel system presented in this thesis. The literature provided many good ideas which could be applied. Two sections of experimental work were conducted. The first was aimed at simply proving the concept of robotically effected sculpting of plastic foams. A crude procedure was developed which proved to be rather tedious and manual, especially in terms of generating the tool paths. Qualitative observations of the cut surfaces were used to change the testing parameters to explore their effects and discover which parameters produced accurate and smooth sculpted surfaces. 12 tests were documented and proved that the sculpting of satisfactory surfaces was achievable. The second section of experimental work involved developing the aforementioned crude procedure to make it more automated, especially in terms of the tool path generation and optimisation step. An innovative five step procedure was developed which if followed can produce accurately sculpted artefacts using CAD models of the artefacts as the primary input. Two artefacts were successfully sculpted using the developed procedure. The first was a simple lofted surface; the CAD model of which was created in SolidWorks. The second artefact was a patient customised medical radiation therapy head and neck support; the CAD model of which was created by scanning the back of the author's head and neck with a 3D scanner. The sculpted support fitted the author perfectly. The implementation of the procedure in the two tests highlighted several points including the speed in which the whole process can be carried out. The time taken from the scanning of the authors head and neck with the 3D scanner through to the physical sculpted artefact, was a mere 80 minutes; of which only 13 minutes was consumed in the actual setup and sculpting step! This is extremely quick when compared to conventional rapid prototyping systems and CNC milling. Several areas of future work were outlined and included, tool and fixture design, automation and integration of the system procedure, tool pathing strategy for foam cutting and robot control system issues. The work presented in this thesis provides an excellent foundation for future development of the robotic foam sculpting system.

The production of highly aerated foods remains a challenge that requires skill to obtain desired appearance and texture. Since foams are fragile and inherently unstable, evaluation of structure changes with time requires a delicate approach. Non-invasive but informative evaluation of changes in aerated food properties is a desired goal to be achieved in this thesis. I studied the aging of egg white foams of different void fraction using two noninvasive techniques - ultrasonic spectroscopy and electrical resistivity - with a view to understanding aging mechanisms that would affect the quality of the final product. To help in interpretation of the results, a commercial high void fraction foam, Gillette (Sensitive), was also analyzed. As a support technique for ultrasound results I used image analysis in order quantitatively evaluate the size of bubbles in the foams. Large increases in attenuation were observed with increasing aging time and frequency. Results were modeled using an effective medium theory originated by Foldy (1945) in order to understand the factors governing foam lifetime and texture. From electrical resistance measurements, liquid fraction changes in an egg white foam column were evaluated quantitatively and qualitatively by using a generalized free drainage model. Although egg-white foams were more prone to changes due to drainage, I observed that for all foams the ultrasonic scaling parameter alfa*lambda/freq was proportional to the square of average bubble size (indicative of a diffusively driven aging process due to disproportionation of bubbles). Slopes of alfa*lambda/freq versus aging time were 6*10-8 and 11*10-8 for egg white foams of void fraction 0.65 and 0.78, respectively, indicating that disproportionation progressed approximately twice as fast in the high void fraction foam. The slopes of alfa*lambda/freq versus aging time were similar for both Gillette foam void fractions (0.93 and 0.91) at a value of 1.5*10–8 s-1, attributable to a lower solubility of isobutane compared to air. By combining ultrasound and electrical resistivity, this thesis has provided novel insights into understanding instability processes occurring in foams. Potentially, ultrasound techniques could be used instead of imaging for foam aging studies, since non-invasive and non-destructive measurements of attenuation and phase velocity permit interrogation of opaque foam structures.

Syntactic foams-composite materials consisting of hollow particles embedded in a host matrix-have many applications for manufactured products, including weight reduction, thermal insulation, and noise reduction. In this thesis, a certain variety of syntactic foam is investigated with regards to reducing fluid borne noise in hydraulic systems. Such a foam maintains stiffness at low hydrostatic pressures and becomes compressible as pressure increases. With this compressibility, the foam is potentially useful as a liner for a reactive noise control device, much like compressed gas style devices currently in use; but the syntactic foam additionally adds significant damping to the system. In order to predict device performance, a linear multimodal model is developed of a hydraulic suppressor, constructed as an expansion chamber lined with a syntactic foam insert. Material models are developed for various compositions of the foam liners, based on an inverse analysis matching the model to experimental results. Two model simplifications are considered, and it is found that a simplified bulk modulus model gives sufficiently accurate results to make approximate predictions of suppressor performance. Several optimizations are performed to predict the optimal material composition for hydraulic excavator work cycles. To help compare the prototype suppressor against commercially available bladder style suppressors, a model is developed for the bladder style silencer and is validated experimentally. Overall, this work both demonstrates the current and potential utility of syntactic foam as a device lining material, and contributes new models to the hydraulics noise control community.

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