The main objective of the thesis called: "Benefits of professional practice for graduates in subject of Public Health Protection at Faculty of Health and Social Studies of University of South Bohemia", was to monitor graduates´ views of quality and benefits of professional practice in this field. As a partial objective I decided to find out whether the practice experience met the expectations of graduates. I set three research hypotheses. The first hypothesis says, the graduates in subject of Public Health Protection rate professional practice as beneficial. The second hypothesis says, the graduates in subject of Public Health Protection rate the professional practice as good. The third and last research hypothesis says, the professional practice met the graduates? expectations. First two hypotheses were fully proved, the third hypothesis was proved only from 50%. My thesis is divided into the theoretical and practical part. The chapter "Current State" was worked out by the method of secondary data analysis. I tried to summarize different fields and issues and to explain concepts related to the study and practice of students. The quantitative method was applied to process empirical research. The method of a questionnaire was applied to gather data. The questionnaire was compiled only for the purpose of this research and has 45 questions. There are closed, open and semi-closed questions in the questionnaire. The research sample consists of graduates in subject of Public Health Protection at Faculty of Health and Social Studies in České Budějovice. The data were gathered from November 2010 till March 2011. Survey was voluntary and anonymous. The questionnaires were distributed to 125 graduates in Public Health Protection via the Internet. The resulting research file consists of 72 respondents (100%). Until now, no literature that would examine this issue has been published. Therefore, the findings are unlikely to confront. This thesis might be a valuable feedback providing matter-of-fact and specific comments of graduates. I hope, this research will be inspiration for improvement of both professional practice and tuition.