Colour is one of the main sensory characteristics of berry juice and fruit products and this parameter also powerfully impacts on consumer behaviour. However, the colour of berry juices is unstable and degradation occurs during storage. The main objectives of the project were: to determine the mechanism by which boysenberry juice enhances the colour of other berry juices and to determine if its addition to berry juices will also stabilise the anthocyanin pigments and enhance copigmentation. In this study, total anthocyanin, total phenolic acids, hyperchromic and bathochromic shift and the rate of colour degradation was measured by spectrophotometric techniques. Individual anthocyanin and phenolic acid content were measured in each juice by high performance liquid chromatography (HPLC) were evaluated during storage at 5, 20 and 35?C. Boysenberry juice improved the colour of blackcurrant, cranberry and pomegranate juices immediately after addition; however, only blackcurrant juice colour was stable during storage at 5?C. There was no influence on the stability of total anthocyanins in pomegranate or cranberry juices when boysenberry juice was added. Of the three juices, pomegranate had the highest rate of degradation. The total anthocyanin of blackcurrant enhanced with boysenberry juice was more stable than for cranberry and pomegranate juices. The impact of phenolic acids found in boysenberry juice (kaemferol, quercetin and ellagic acid) on blackcurrant juice colour stability was also investigated. The colour stability of blackcurrant juice was improved by the addition of ellagic acid at 5?C; however, the colour intensity of blackcurrant enhanced with kaemferol and quercetin decreased with storage. The copigmentations between anthocyanins themselves were not found to be a significant effect on colour stability of blackcurrant juice. Ellagic acid had the strongest colour improvement in blackcurrant juice compared to boysenberry juice. In conclusion, ellagic acid as found in boysenberry juice formed intermolecular copigmentation with blackcurrant juice anthocyanins, so this resulted in stabilised juice colour during storage; however, the effect was found when the juice was stored at 5?C only.

Content removed due to copyright restrictions: Smale, N.J. Tanner D.J., Amos N.D., Cleland D.J. (2003). Airflow properties of packaged horticultural produce - a practical study. Acta Horticulturae, (599), 443-450 / Horticultural exports are of economic significance to New Zealand. Only through providing consistently high quality products to distant markets can New Zealand hope to command a premium price. New Zealand's two major horticultural exports, apples and kiwifruit, are transported to foreign markets by sea; either in refrigerated holds on-board cargo vessels or in refrigerated containers. Long transit times mean that conditions in these systems must be carefully controlled to ensure high quality product arrives at market. Effective distribution of air is a key consideration in transport systems. A mathematical model to describe the flow of air in marine transport systems was developed. The model was based on a resistance network framework, relying on simplification of the complex geometry within the refrigerated space to a discrete number of flow paths and points of convergence and divergence. Correlations quantifying the flow resistance of each channel were required. Some of these correlations were already available, and some were developed specifically for this purpose. A general method for predicting the flow resistance of enclosed conduits based on the Darcy-Weisbach, laminar and Colebrook equations was found to be sufficiently accurate for use. The flow resistance of horizontally vented horticultural packages was quantified and the cause of the flow resistance investigated. Entrance and exit effects were found to be significant, and a relationship between vent size and flow resistance was developed. Air interchange between a vented carton and the general refrigerated space was shown to be a significant mode of heat transfer. The effect of vent design on the rate of air interchange was found to be complex. Quantitative relationships between vent characteristics and rates of air interchange could not be developed; however, some general observations were made. Vent size, aspect ratio and alignment were all found to affect the rate of interchange. An existing method for determining in-package fluid velocities was refined to improve the accuracy of data and reduce the measurement time. A low-cost method for measuring airflows in transport systems was also developed utilising thermistors. These thermistor anemometers were used to monitor velocities in four shipments of fresh produce from New Zealand. Three of the four vessels monitored showed large variation in the circulation rate in the period between evaporator defrosts due to frosting. In some cases, frosting was severe enough to cause loss of delivery air temperature control. Management of defrosts was identified as an area of improvement in refrigerated hold management. Validation of the model developed was performed using four systems: a laboratory scale test-rig, a 40' container and two of the surveyed refrigerated holds. Airflow predictions were used with a heat transfer model to predict in-package temperatures. Comparison of measured and predicted flows and in-package temperatures showed good agreement given uncertainty of geometry and input data. The implications of altering a number of operational and design variables in both containers and refrigerated holds were investigated using the developed models. Increased circulation rates were found to increase cooling rates and reduce temperature variability in both types of systems; however, the magnitude of the benefit decreased with increasing circulation rate. Removal of the floor gratings and the use of pallet bases as an air distribution channel was found to increase temperature variability in both types of systems. The magnitude of the increase was small in a 40' container but substantial in a refrigerated hold. The correlations and models developed in this thesis provide useful tools to analyse and optimise the design and operation of refrigerated marine transport systems.

<div><div><div><p>Interfacial rheology describes the functional relationship between the deformation of an interface, the stresses exerted in and on it, and the resulting flows in the adjacent fluid phases. These interfacial properties are purported to influence emulsion stability. Protein emulsifiers tend to adsorb to the interface of immiscible phases, reduce interfacial tension as well as generate repulsive interactions. A magnetic interfacial shear rheometer was used to characterize the surface pressure-area isotherms as well as interfacial rheological properties of two proteins- sodium caseinate and b-lactoglobulin. Then, sodium caseinate was used as a carrier for b-carotene encapsulation.</p><p>b-carotene is a carotenoid that exhibits pro-vitamin A activity, antioxidant capacity and is widely used as a food colorant. It is however, highly hydrophobic and sensitive to heat, oxygen and light exposure. Thus b-carotene as food ingredient is mainly available as purified crystals or as oil-in-water emulsions. In this study, b-carotene stability, and solubility in water for application as a natural colorant was improved by preparation of a sodium caseinate/ b-carotene powder using high pressure homogenization, solvent evaporation and spray drying. The powders thus prepared showed good solubility in water and yielded an orange coloration from b-carotene. The effect of medium chain triglyceride concentration (1%, 10%) and incorporation of a natural antioxidant (Duralox, Kalsec) on powder stability was studied as a function of storage time and temperature.b-carotene stability was reduced at higher storage temperature (4oC> 21oC> 50oC) over 60 days and followed first order degradation kinetics at all temperatures. Incorporation of natural antioxidant improved b-carotene stability and resulted in a second first order degradation period at 50oC. As b-carotene content decreased, Hunter Lab color values denoting lightness increased, while those for redness and yellowness of the powder decreased. This sodium caseinate based b-carotene powder could be used as a food ingredient to deliver natural b-carotene to primarily aqueous food formulations.</p><div><div><div><p>In the last part of this work, an engagement workshop was developed as a means to educate young consumers about the function of emulsifiers in foods. Food additives are important for food product development, however to consumers, a discord between their objective purpose and subjective quality has led to confusion. Food emulsifiers, in particular, are associated with lower healthiness perception due to their unfamiliar names. In collaboration with the 4H Academy at Purdue, a workshop high school student was conducted to develop an increased understanding of emulsions and emulsifiers. A survey was conducted with the participants who self-evaluated their gain in knowledge and tendency to perform certain behaviors with regards to food ingredient labels. The participants reported a gain in knowledge in response to four key questions on emulsions and emulsifiers, as well as an increased likelihood to read ingredients on a food label and look up information on unfamiliar ingredients.</p></div></div></div></div></div></div>

<div>The House Cricket (Acheta domesticus) is a promising alternative to traditional protein sources, as these insects produce over 12 times the mass of protein for a given mass of food/water when compared to cattle, while also producing lower amounts of greenhouse gases and NH3 emissions (Kim et al. 2017, Hanboonsong, Jamjanya and Durst 2013, Van Huis 2013). Additionally, previous studies have demonstrated significant emulsification and gelling properties of insect flours, such as from cricket, which has been attributed to the functional properties of the protein (Kim et al. 2017). Ground cricket flours contain significant quantities of both protein and fibrous polysaccharides, particularly chitin. Since chitin particles are also capable of preparing emulsions as a Pickering stabilizer, there remains a question on the relative role of the protein and chitin components in crickets for stabilizing emulsion products. Relative contributions of each component was identified by first isolating the water-soluble protein and water-insoluble chitin fractions from ground cricket flour and then determining their interfacial properties and stability of prepared oil-in-water emulsions. Dynamic interfacial tension measurements indicated significant surface activity of the protein fraction, while there was minimal evidence of significant surface pressure development in the presence of 5-10 μm chitin particles. 10 % (w/w) canola oil-in-water emulsions were prepared with 0.5-2% (w/w) of the water-soluble protein fraction and 5.29% (w/w) canola oil-in-water emulsions were prepared with 0.688% of the chitin fraction. Stability of the emulsions against creaming was between 75% and 90% for emulsions stabilized by the protein fraction over three weeks of storage and between 93% and 96% for emulsions stabilized by chitin over 24 hours of storage. Significant fractions of precipitate- and oil-layers found in chitin-stabilized dispersions was attributed to the presence of large chitin particles (79 μm volume weighted mean diameter) and inefficient adsorption to droplet interfaces during homogenization, respectively. Volume-weighted mean diameter of emulsified oil droplets remained at 17-24 μm among protein-stabilized (>1.5 wt%) emulsions over three weeks of storage but only 60 μm over 24 hours among chitin-stabilized emulsions. Light micrographs of emulsion droplets showed successful adsorption of chitin fractions to oil droplets in the emulsion layer, verifying their potential as Pickering stabilizers. These findings demonstrated that both water-soluble protein and chitin particles obtained from ground cricket flours are legitimate emulsion stabilizers, yet the chitin fraction is much less effective without a more intensive approach to reduce their particle size.</div>

<div>Zein is major nitrogen storage protein that accounts for nearly half of the protein content of the corn grain. As a byproduct of starch and ethanol processing, it is generally recognized as safe (GRAS) and soluble in up to 70% ethanol. Historically, zein has been used for films and coatings. However, usage of the corn protein has diminished in recent years. New advances in food nanotechnology has renewed interest in zein. By forming the protein into stable nanoparticles capable of being dispersed in aqueous solution, zein can be used in many applications ranging from improving stability and digestion of functional ingredients or active biodegradable packaging. Developing novel applications for this protein would then add value to a waste product during the processing of corn.</div><div><br></div><div>The formation of hydrophobic zein nanoparticles (ZNPs) would allow for easier dispersion in aqueous systems without further modification to increase hydrophilicity. However, their dispersibility and subsequent stability in aqueous systems is important for its functionality in food. Addition of sufficient ι-carrageenan (ι-CGN) prevented aggregation in the pH range of 5.25 to 6.75 and limited aggregation at pH 7.0. Enhanced stability was attributed to the adhesion of ι-CGN to the nanoparticle surface, as the ZNPs surface charge became significantly negative with introduction of ι-CGN. These particles remained stable for up to 30 days with significantly lower turbidity and greater resistance to gravitational separation when compared to ZNPs alone.</div><div><br></div><div>Lutein was encapsulated in zein nanoparticles, and the bioaccessibility was determined by quantifying lutein content after exposure to in vitro gastric and intestinal conditions. It was found that ZNPs provided a protective environment for lutein in aqueous dispersions and would release the carotenoid into the small intestine by rapid breakdown of ZNP structure during intestinal digestion. However, the process or residual components must have limited uptake of lutein into mixed micelles. ZNPs can be a promising encapsulating agent to improve the digestive stability of lutein.</div><div><br></div><div>Composite films composed of methylcellulose (MC) and zein nanoparticles (ZNPs) were prepared as a potential biodegradable alternative for synthetic packaging. The effects of ZNP aggregation on mechanical and moisture barrier properties as affected by drying temperature, pH, and stabilizers were tested. The phase separation of composite films was determined to be detrimental to both its mechanical and moisture barrier properties. The drying temperature, pH, and composition of the solvent casting solution all affected the distribution of ZNPs dispersed in MC films. Drying films at 23°C or setting the pH to 6.5 resulted in ZNP aggregation and weaker, brittle films that were poor moisture barriers. The presence of CGN was able to provide stability to ZNPs at both pH 4 and 6.5, thus improving its mechanical and moisture barrier properties.</div>

No description available.

No description available.

Emulsions form a major part of processed food formulations, either being the end products in themselves or as parts of a more complex food system. For the past few decades, colloid scientists have focussed mainly on the effects of processing conditions (e.g. heat, high pressure, and shear) on the physicochemical properties of emulsions (e.g. viscosity, droplet size distribution and phase stability). However, the information about the behaviour of food structures post consumption is very limited. Fundamental knowledge of how the food structures behave in the mouth is critical, as these oral interactions of food components influence the common sensorial perceptions (e.g. creaminess, smoothness) and the release of fat-soluble flavours. Initial studies also suggest that the breakdown of emulsions in the gastrointestinal tract and the generated interfacial structures impact lipid digestion, which can consequently influence post-prandial metabolic responses. This area of research needs to be intensively investigated before the knowledge can be applied to rational design of healthier food structures that could modulate the rate of lipid metabolism, bioavailability of nutrients, and also help in providing targeted delivery of flavour molecules and/or bioactive components. Hence, the objective of this research was to gain understanding of how emulsions behave during their passage through the gastrointestinal tract. In vitro digestion models that mimic the physicochemical processes and biological conditions in the mouth and gastrointestinal tract were successfully employed. Behaviour of model protein-stabilized emulsions (both positively charged (lactoferrin) as well as negatively charged [β-lactoglobulin (β-lg)] oil-in-water emulsions) at each step of simulated physiological processing (using model oral, gastric and duodenal fluids individually) were investigated. In simulated mouth conditions, oil-in-water emulsions stabilized by lactoferrin or β-lg at the interfacial layers were mixed with artificial saliva at neutral pH that contained a range of mucin concentrations and salts. The β-lg emulsions did not interact with the artificial saliva due to the dominant repulsion between mutually opposite charges of anionic mucin and anionic β-lg interfacial layer at neutral pH. However, β-lg emulsions underwent some depletion flocculation on addition of higher concentrations of mucin due to the presence of unadsorbed mucin molecules in the continuous phase. In contrast, positively charged lactoferrin emulsions showed considerable salt-induced aggregation in the presence of salts (from the saliva) alone. Furthermore, lactoferrin emulsions underwent bridging flocculation because of electrostatic binding of anionic mucin to the positively charged lactoferrin-stabilized emulsion droplets. In acidic pH conditions (pH 1.2) of the simulated gastric fluid (SGF), both protein-stabilized emulsions were positively charged. Addition of pepsin resulted in extensive droplet flocculation in both emulsions with a greater extent of droplet instability in lactoferrin emulsions. Coalescence of the droplets was observed as a result of peptic hydrolysis of the interfacial protein layers. Conditions such as ionic strength, pH and exposure to mucin were shown to significantly influence the rate of hydrolysis of β-lg-stabilized emulsion by pepsin. Addition of simulated intestinal fluid (SIF) containing physiological concentrations of bile salts to the emulsions showed competitive interfacial displacement of β-lg by bile salts. In the case of lactoferrin-stabilized emulsion droplets, there was considerable aggregation in the presence of intestinal electrolytes alone (without added bile salts) at pH 7.5. Binding of anionic bile salts to cationic interfacial lactoferrin layer resulted in re-stabilization of salt-aggregated lactoferrin emulsions. On mixing with physiological concentrations of pancreatin (mixture of pancreatic lipase, amylase and protease), significant degree of coalescence and fatty acid release occurred for both the emulsions. This was attributed to the interfacial proteolysis by trypsin (proteolytic fractions of pancreatin) resulting in interfacial film rupturing. Exchange of initial interfacial materials by bile salts and trypsin-induced film breakage enhanced the potential for lipolytic fractions of pancreatin to act on the hydrophobic lipid core. The lipid digestion products (free fatty acids and mono and/or diglycerides) generated at the droplet surface further removed the residual intact protein layers from the interface by competitive displacement mechanisms. The sequential treatment of the cationic and anionic emulsions with artificial saliva, SGF and SIF, respectively, was determined to understand the impact of initial protein type during complete physiological processing from mouth to intestine. Broadly, both the protein-stabilized emulsions underwent charge reversals, extensive droplet flocculation, and significant coalescence as they passed through various stages of the in vitro digestion conditions. Except in the simulated mouth environment, the initial charge of the emulsifiers had relatively limited influence on droplet behaviour during the simulated digestion. The results contribute to the knowledge of how structure and charge of the emulsified lipid droplets impact digestion at various stages of physiology. This information might have important consequences for developing suitable microstructures that allow controlled breakdown of droplets in the mouth and predictable release of lipids in the gastrointestinal tract.

Emulsions form a major part of processed food formulations, either being the end products in themselves or as parts of a more complex food system. For the past few decades, colloid scientists have focussed mainly on the effects of processing conditions (e.g. heat, high pressure, and shear) on the physicochemical properties of emulsions (e.g. viscosity, droplet size distribution and phase stability). However, the information about the behaviour of food structures post consumption is very limited. Fundamental knowledge of how the food structures behave in the mouth is critical, as these oral interactions of food components influence the common sensorial perceptions (e.g. creaminess, smoothness) and the release of fat-soluble flavours. Initial studies also suggest that the breakdown of emulsions in the gastrointestinal tract and the generated interfacial structures impact lipid digestion, which can consequently influence post-prandial metabolic responses. This area of research needs to be intensively investigated before the knowledge can be applied to rational design of healthier food structures that could modulate the rate of lipid metabolism, bioavailability of nutrients, and also help in providing targeted delivery of flavour molecules and/or bioactive components. Hence, the objective of this research was to gain understanding of how emulsions behave during their passage through the gastrointestinal tract. In vitro digestion models that mimic the physicochemical processes and biological conditions in the mouth and gastrointestinal tract were successfully employed. Behaviour of model protein-stabilized emulsions (both positively charged (lactoferrin) as well as negatively charged [β-lactoglobulin (β-lg)] oil-in-water emulsions) at each step of simulated physiological processing (using model oral, gastric and duodenal fluids individually) were investigated. In simulated mouth conditions, oil-in-water emulsions stabilized by lactoferrin or β-lg at the interfacial layers were mixed with artificial saliva at neutral pH that contained a range of mucin concentrations and salts. The β-lg emulsions did not interact with the artificial saliva due to the dominant repulsion between mutually opposite charges of anionic mucin and anionic β-lg interfacial layer at neutral pH. However, β-lg emulsions underwent some depletion flocculation on addition of higher concentrations of mucin due to the presence of unadsorbed mucin molecules in the continuous phase. In contrast, positively charged lactoferrin emulsions showed considerable salt-induced aggregation in the presence of salts (from the saliva) alone. Furthermore, lactoferrin emulsions underwent bridging flocculation because of electrostatic binding of anionic mucin to the positively charged lactoferrin-stabilized emulsion droplets. In acidic pH conditions (pH 1.2) of the simulated gastric fluid (SGF), both protein-stabilized emulsions were positively charged. Addition of pepsin resulted in extensive droplet flocculation in both emulsions with a greater extent of droplet instability in lactoferrin emulsions. Coalescence of the droplets was observed as a result of peptic hydrolysis of the interfacial protein layers. Conditions such as ionic strength, pH and exposure to mucin were shown to significantly influence the rate of hydrolysis of β-lg-stabilized emulsion by pepsin. Addition of simulated intestinal fluid (SIF) containing physiological concentrations of bile salts to the emulsions showed competitive interfacial displacement of β-lg by bile salts. In the case of lactoferrin-stabilized emulsion droplets, there was considerable aggregation in the presence of intestinal electrolytes alone (without added bile salts) at pH 7.5. Binding of anionic bile salts to cationic interfacial lactoferrin layer resulted in re-stabilization of salt-aggregated lactoferrin emulsions. On mixing with physiological concentrations of pancreatin (mixture of pancreatic lipase, amylase and protease), significant degree of coalescence and fatty acid release occurred for both the emulsions. This was attributed to the interfacial proteolysis by trypsin (proteolytic fractions of pancreatin) resulting in interfacial film rupturing. Exchange of initial interfacial materials by bile salts and trypsin-induced film breakage enhanced the potential for lipolytic fractions of pancreatin to act on the hydrophobic lipid core. The lipid digestion products (free fatty acids and mono and/or diglycerides) generated at the droplet surface further removed the residual intact protein layers from the interface by competitive displacement mechanisms. The sequential treatment of the cationic and anionic emulsions with artificial saliva, SGF and SIF, respectively, was determined to understand the impact of initial protein type during complete physiological processing from mouth to intestine. Broadly, both the protein-stabilized emulsions underwent charge reversals, extensive droplet flocculation, and significant coalescence as they passed through various stages of the in vitro digestion conditions. Except in the simulated mouth environment, the initial charge of the emulsifiers had relatively limited influence on droplet behaviour during the simulated digestion. The results contribute to the knowledge of how structure and charge of the emulsified lipid droplets impact digestion at various stages of physiology. This information might have important consequences for developing suitable microstructures that allow controlled breakdown of droplets in the mouth and predictable release of lipids in the gastrointestinal tract.

Emulsions form a major part of processed food formulations, either being the end products in themselves or as parts of a more complex food system. For the past few decades, colloid scientists have focussed mainly on the effects of processing conditions (e.g. heat, high pressure, and shear) on the physicochemical properties of emulsions (e.g. viscosity, droplet size distribution and phase stability). However, the information about the behaviour of food structures post consumption is very limited. Fundamental knowledge of how the food structures behave in the mouth is critical, as these oral interactions of food components influence the common sensorial perceptions (e.g. creaminess, smoothness) and the release of fat-soluble flavours. Initial studies also suggest that the breakdown of emulsions in the gastrointestinal tract and the generated interfacial structures impact lipid digestion, which can consequently influence post-prandial metabolic responses. This area of research needs to be intensively investigated before the knowledge can be applied to rational design of healthier food structures that could modulate the rate of lipid metabolism, bioavailability of nutrients, and also help in providing targeted delivery of flavour molecules and/or bioactive components. Hence, the objective of this research was to gain understanding of how emulsions behave during their passage through the gastrointestinal tract. In vitro digestion models that mimic the physicochemical processes and biological conditions in the mouth and gastrointestinal tract were successfully employed. Behaviour of model protein-stabilized emulsions (both positively charged (lactoferrin) as well as negatively charged [β-lactoglobulin (β-lg)] oil-in-water emulsions) at each step of simulated physiological processing (using model oral, gastric and duodenal fluids individually) were investigated. In simulated mouth conditions, oil-in-water emulsions stabilized by lactoferrin or β-lg at the interfacial layers were mixed with artificial saliva at neutral pH that contained a range of mucin concentrations and salts. The β-lg emulsions did not interact with the artificial saliva due to the dominant repulsion between mutually opposite charges of anionic mucin and anionic β-lg interfacial layer at neutral pH. However, β-lg emulsions underwent some depletion flocculation on addition of higher concentrations of mucin due to the presence of unadsorbed mucin molecules in the continuous phase. In contrast, positively charged lactoferrin emulsions showed considerable salt-induced aggregation in the presence of salts (from the saliva) alone. Furthermore, lactoferrin emulsions underwent bridging flocculation because of electrostatic binding of anionic mucin to the positively charged lactoferrin-stabilized emulsion droplets. In acidic pH conditions (pH 1.2) of the simulated gastric fluid (SGF), both protein-stabilized emulsions were positively charged. Addition of pepsin resulted in extensive droplet flocculation in both emulsions with a greater extent of droplet instability in lactoferrin emulsions. Coalescence of the droplets was observed as a result of peptic hydrolysis of the interfacial protein layers. Conditions such as ionic strength, pH and exposure to mucin were shown to significantly influence the rate of hydrolysis of β-lg-stabilized emulsion by pepsin. Addition of simulated intestinal fluid (SIF) containing physiological concentrations of bile salts to the emulsions showed competitive interfacial displacement of β-lg by bile salts. In the case of lactoferrin-stabilized emulsion droplets, there was considerable aggregation in the presence of intestinal electrolytes alone (without added bile salts) at pH 7.5. Binding of anionic bile salts to cationic interfacial lactoferrin layer resulted in re-stabilization of salt-aggregated lactoferrin emulsions. On mixing with physiological concentrations of pancreatin (mixture of pancreatic lipase, amylase and protease), significant degree of coalescence and fatty acid release occurred for both the emulsions. This was attributed to the interfacial proteolysis by trypsin (proteolytic fractions of pancreatin) resulting in interfacial film rupturing. Exchange of initial interfacial materials by bile salts and trypsin-induced film breakage enhanced the potential for lipolytic fractions of pancreatin to act on the hydrophobic lipid core. The lipid digestion products (free fatty acids and mono and/or diglycerides) generated at the droplet surface further removed the residual intact protein layers from the interface by competitive displacement mechanisms. The sequential treatment of the cationic and anionic emulsions with artificial saliva, SGF and SIF, respectively, was determined to understand the impact of initial protein type during complete physiological processing from mouth to intestine. Broadly, both the protein-stabilized emulsions underwent charge reversals, extensive droplet flocculation, and significant coalescence as they passed through various stages of the in vitro digestion conditions. Except in the simulated mouth environment, the initial charge of the emulsifiers had relatively limited influence on droplet behaviour during the simulated digestion. The results contribute to the knowledge of how structure and charge of the emulsified lipid droplets impact digestion at various stages of physiology. This information might have important consequences for developing suitable microstructures that allow controlled breakdown of droplets in the mouth and predictable release of lipids in the gastrointestinal tract.