Call number: LD2668 .T4 1963 K57 / Master of Science

Call number: LD2668 .T4 1965 A31 / Master of Science

Thesis (PhD.(Animal Science) North-West University, Mafikeng Campus, 2012

No description available.

No description available.

No description available.

Listeria monocytogenes is the causative agent of listeriosis, a severe foodborne infection that is increasing significantly in Europe and North America. A correlating factor contributing to the resurgence of listeriosis is the rise in consumption of cold-stored ready-to-eat (RTE) foods. The steady upsurge in disease requires more focused research to control the pathogen, L. monocytogenes. Currently, there is a plethora of diagnostic methods for the causative agent, however, each has limitations, one of which is the inability to correlate results across laboratories. This is a particular hindrance to an outbreak investigation in an age when food is transported widely across the globe. In this study, proteomic approaches were used to search for biomarkers that facilitate rapid characterisation of isolates against a background of differentially expressed proteins. A preamble to this investigation necessitated incorporation of an efficient lysis procedure to release maximum proteins. This was eventually achieved using a Listeria specific enzyme, endolysin, and a disruptive mechanical method. Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) data showed that bead beating and enzymatic lysis were the most efficient methods for analysis of the proteome. Dendrogram lineages, derived from MALD-TOF-MS spectra, strongly correlated with 16S rRNA analyses. Selective protein capture and analysis by MALD-TOF-MS (designated SELDI-TOF-MS) demonstrated considerable intraspecies diversity as revealed by dendrograms which were also visualised by „Heat Maps‟. One-dimensional polyacrylamide gel electrophoresis and LC-MS/MS analysis of seven L. monocytogenes isolates, led to the successful identification of two proteins; a hypothetical protein, designated lwe06778 and a phosphoribosyl-AMP cyclohydrolase which were uniquely present at 4°C. This finding suggests that L. monocytogenes depends on the histidine biosynthesis pathway in order to survive at cold temperatures. It is hypothesised that the addition of inhibitors, specific to both proteins in RTE cold foods may be a useful means for controlling outbreaks of listeriosis in the future.

The aim of the study was to investigate which routines preschools had regarding food processing and if the routines were sufficient in order to the regulation. The aim was also to investigate if there were some difference in food processing depending on the employee´s education. The method used was an interview study of 20 preschools. The results indicated that the majority of the preschools had sufficient routines regarding temperature control of hot and cold lunches. Regarding measurement on deviant temperature, half of the preschools routines were sufficient. 55 % of the preschools had insufficient routines for temperature control in cold storage. Results indicate that it was differences in how often the employee´s control the temperature in food depended on the employee´s education. The conclusion is that a number of preschools had a capacity for improvement of their routines. The preschools have to work with prevention measures to create routines which is sufficient in order to the regulation.

Thesis (M.S.)--University of Wisconsin--Madison, 1965. / eContent provider-neutral record in process. Description based on print version record. Bibliography: l. 126-130.

Thesis (M.S.)--University of Wisconsin--Madison, 1965. / eContent provider-neutral record in process. Description based on print version record. Bibliography: l. 78-85.