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Forage may undergo extensive proteolysis during fermentation. The objectives of this study were to determine if treatment of forage with heat can reduce proteolysis during subsequent fermentation. In Experiment 1, direct-cut barley and alfalfa were either microwaved or steamed then ensiled in laboratory silos as were untreated and wilted forage. Silages of microwaved or steamed forage showed marked increase in NDIN and recovery of hot water insoluble N; however, alfalfa silages also had high pH and butyric acid. In Experiment 2, steaming was compared to formic acid and anhydrous ammonia treatments for their ability to prevent proteolysis in alfalfa silages. Steamed and ensiled alfalfa also was evaluated with addition of microbial inoculant or formic acid. Silage of steamed alfalfa had greater NDIN and recovery of precipitable N than controls, formic acid, or ammonia treated silage. There was no difference in precipitable N between formic acid and ammonia treatments. Silage of steam treatment had lower pH than wilted or direct-cut controls, and additives to steamed forage favored a more homolactic fermentation. Additives to steamed forage also increased aerobic stability of the silage. Steamed silage had less aerobic stability than direct-cut silage. Rumen degradability of silage CP and OM from both experiments were evaluated. In Experiment 1, CP degradability of microwaved or steamed silages was 8 to 26% less than unheated silages, but all had similar undegraded CP after incubation for 72 h. In Experiment 2, wilting, steam, formic acid and ammonia treatments had similar, but decreased CP degradability when compared to direct-cut silage. Longer duration heat in Experiment 1 resulted in greater silage protein preservation, and greater decrease in rumen degradability of CP than Experiment 2. / Master of Science

Thesis (MSc. (Agriculture Pasture Science)) -- University of Limpopo, 2018 / This study was conducted at the University of Limpopo’s Experimental Farm (Syferkuil), in the Limpopo Province, South Africa. The objective was to determine the effect of foliar fertilizers on the dry matter production over a period of two growing seasons. The experiment was laid out in a 3 x 3 factorial arrangement, fitted in a randomized complete block design (RCBD), with 4 replications. The experiment was planted on 28th March 2014 for season 1 (2014) and 31st March 2015 for season 2 (2015). Three sources of fertilizer (Nitrospray Plus, Potaspray and a combination of the two) were applied to stooling rye at three different fertilizer application rates (0 kg or ℓ/ha, 2.5 kg or ℓ/ha and 5.0 kg or ℓ/ha). The Nitrospray Plus and Potaspray fertilizers were applied on the 09th May, 02nd July, 27th August and 30th October 2014 for Season 1 (2014). During the Season 2 (2015), they were applied on 11th May, 04th July, 29th August and 02nd November 2015. The application was done using a knapsack sprayer. Above-ground biomass was harvested four times during each season when the plants reached a height of 25–30 cm. During 2014, harvesting was done on the 27th May, 24th July, 12th September and the 17th November 2014. During 2015, harvesting was done on the 29th May, 26th July, 14th September and the 19th November 2015. The dry matter production was determined by harvesting 1 m2 quadrates at 3 cm height. After weighing fresh material, it was oven–dried at 55oC until a constant mass was reached. Samples were then weighed again and the DM production (kg/ha) was calculated. Results were compared, using analysis of variance and the Fisher’s LSD test at P≤ 0.05 within each season. Based on the Least Significance Difference test, treatments were grouped in different production classes. During both seasons, fertilizer application rates had a significant (P≤0.001) influence on dry matter production. In 2014, the highest total dry matter production obtained was 5323 kg/ha in the high potassium fertilizer treatment and the lowest total dry matter was 4049 kg/ha in the control or zero application treatment. xiii In 2015, the highest total dry matter production obtained was 5595 kg/ha in the high potassium fertilizer treatment and the lowest 3678 kg/ha in the control treatment. Potassium fertilizers produced significantly (P≤0.001) higher than nitrogen fertilizers in both seasons. The main conclusions from this study were that high foliar fertilizer application rates (5.0 kg/ha) led to high, low foliar application rates (2.5 kg/ha) to lower and no fertilizer to low dry matter production. High levels of potassium produced the best, followed by combinations of intermediate potassium and nitrogen, and high nitrogen application rates. Yields of above 5000 kg/ha were obtained under high potassium rates. Lower rates led to total productions of more than 4000 kg/ha, while no foliar application produced between 3700 and 4000 kg/ha. It was also concluded that stooling rye is a suitable winter fodder crop for the Pietersburg Plateau Bushveld, but that the application of fertilizers is necessary for increased dry matter production. Both and Nitrospray and Potaspray Plus, as individual foliar fertilizers or in combination, can be applied to increase the dry matter production of stooling rye. However, it is essential to investigate the adaptability of stooling rye in other areas of the Limpopo bushveld, to determine its suitability as a fodder crop in other areas in the province. Keywords: Dry matter production, cutting dates, foliar fertilizers, Nitrospray Plus, Potaspray.

MSCAGR (Animal Science) / Department of Animal Science / Forage and browse legumes play an important role in sustaining livestock in small holder farming systems in the tropics, mainly as a result of their contribution to economic and environmental sustainability of livestock production. The study was conducted to determine the chemical composition, ruminal degradability and in vitro digestibility of dry matter (DM) and crude protein (CP) of Dichrostachys cinerea and Bauhinia thonningii leaves. The browse tree leaves were harvested in the wild in Shayandima, Limpopo province. The leaves were collected, oven-dried, milled to pass through a 1.0 mm sieve and analysed for chemical composition in the Animal Science Nutrition Laboratory, at the University of Venda. The browse tree leaves were analysed for DM nitrogen, neutral detergent fibre (NDF) and acid detergent fibre (ADF). Approximately 5 g of leaf sample milled to pass through through a 1 mm sieve were placed in nylon bags (external dimension: 6 × 12 cm, pore size of 41 μm) and incubated in duplicates for 0, 4, 8, 16, 24, 48, 72, 96 and 120 hours periods in the rumen of three cannulated Bonsmara steers. The residues were then analysed for DM and nitrogen. Parameters to describe the dynamics of ruminal degradability of DM and CP were obtained by fitting the data on the exponential equation P = a + b (1 - e-ct) using NEWAY computer program, where “a” is the rapid degradable fraction, “b” is the slow degradable fraction and “c” is the outflow rate. The in vitro DM and CP degradability of rumen undegradable residue collected after 24 and 48 hour incubation was determined by sequential in vitro digestion in pepsin (abomasal) and pancreatin (small intestine) solutions. DM and CP content differed significantly (P ˂ 0.05). D. cinerea leaves had higher levels of DM and CP content than B. thonningii leaves. DM and CP disappearance increased (P < 0.01) as the incubation period increased. There was no difference (P > 0.05) in soluble fraction ‘a’ and ‘b’ of DM of the two species. The CP components for both fraction ‘a’ and ‘b’ differed significantly (P < 0.01) for CP among the two species. There was significant difference (P < 0.01) in post-ruminal digestibility among the two species. CP digestibility of B. thonningii and D. cinerea leaves was reduced (P < 0.01). In conclusion, B. thonningii and D. cinerea leaves showed significant difference based on their fermentation kinetics and in vitro digestibility, suggesting a good nutritional quality which can be used as protein source for ruminants in dry season and supplement to low-quality diets. / NRF