published_or_final_version / Biological Sciences / Master / Master of Philosophy

Thesis (M.E.S.)--The Evergreen State College, 2007. / Title from title screen viewed (4/7/2008). Includes bibliographical references (leaves 49-56).

published_or_final_version / Ecology and Biodiversity / Doctoral / Doctor of Philosophy

Arid and semiarid river systems in Western Queensland, Australia, are characterized by the unpredictable and highly variable nature of their hydrological regimes as a result of the episodic nature of rain events in the region. These dryland rivers typically experience episodic floods and extremely low or no flow periods. During low or no flow periods, water persists only in relatively wide and deep sections of the river channels, which are called 'waterholes'. These isolated waterholes serve as refugia for aquatic species during protracted intervals between floods. In such discontinuous riverine habitat, dispersal of freshwater species may be achieved only during wet seasons, when water is flowing in rivers and the nearby floodplains. Obligate aquatic species occur in habitats that represent discrete sites surrounded by inhospitable terrestrial landscapes. Thus, movements are very much limited by the physical nature and arrangement of the riverine system. In addition, the distribution of a species may be also largely dependent on historical events. Landscape and river courses continually change over geological time, often leaving distinct phylogenetic 'signatures', useful in reconciling species' biology with population connectivity and earth history. The main aim of this study was to resolve the relative importance of contemporary and historical processes in structuring populations of two freshwater species in Western Queensland river systems. To address this aim, a comparative approach was taken in analysing patterns of genetic variation of two freshwater invertebrates: a snail (Notopala sublineata) and a prawn (Macrobrachium australiense). Mitochondrial sequences were used for both the species. In addition, allozyme and microsatellites markers were employed for N. sublineata. These species have similar distributions in Western Queensland region, although N. sublineata appears to be extinct in some catchments. M. australiense is thought to have good dispersal abilities due to a planktonic larval phase in its life cycle and good swimming capabilities, whereas N. sublineata is thought to have limited dispersal abilities, because of its benthic behaviour and because this species is viviparous. It was hypothesised that these freshwater invertebrates, would display high levels of genetic structure in populations, because physical barriers represented by terrestrial inhospitable habitat, are likely to impede gene flow between populations inhabiting isolated river pools. Genetic data for the two species targeted in this study supported this hypothesis, indicating strong population subdivision at all spatial scales investigated (i.e. between and within catchments). This suggests that contemporary dispersal between isolated waterholes is relatively restricted, despite the potential good dispersal abilities of one of the species. It was hypothesised that levels of gene flow between populations of aquatic species were higher during the Quaternary (likely movements of individuals across catchment boundaries) and that they have been isolated relatively recently. There is evidence that historically gene flow was occurring between populations, suggesting that episodic dispersal across catchment boundaries was likelier in the past. Episodic historical movements of aquatic fauna were facilitated by higher patterns of river connectivity as a result of the climate changes of the Pleistocene. Because the two species targeted in this study exhibit analogous spatial patterns of evolutionary subdivision it is likely that they have a shared biogeographic history. The unpredictable flow regime of rivers in Western Queensland is likely to have considerable effects on the genetic diversity of aquatic populations. First, if populations of obligate freshwater organisms inhabiting less persistent waterholes are more likely to experience periodic bottlenecks than those inhabiting more persistent ones, they would be expected to have lower levels of genetic diversity. Second, if populations inhabiting less persistent waterholes periodically undergo local extinction with subsequent recolonisation, there should be higher levels of genetic differentiation among them, due to the founder effects, than among those populations inhabiting more persistent waterholes. Contrary to the first prediction, the observed levels of genetic diversity in both N. sublineata and M. australiense were high in both more persistent and less persistent waterholes. There was no tendency for genetic diversity to be lower in less persistent than in more persistent waterholes. However, when Cooper waterholes were ranked in order of persistence, positive correlation between water persistence time in waterholes and genetic diversity was detected in N. sublineata but not in M. australiense. Contrary to the second prediction, highly significant genetic differentiation was found among populations from both less persistent and more persistent waterholes. This indicates that not only populations from less persistent but also those from more persistent waterholes were very dissimilar genetically. This study demonstrated the importance of both contemporary and historical processes in shaping the population structure of obligate freshwater species in Western Queensland river systems. It has indicated that contemporary movements of freshwater species generally are extremely limited across the region, whereas episodic dispersal across catchment boundaries was possible during the Pleistocene, due to different patterns of river connectivity.

Invertebrate spatial patterns were investigated in 36 and 12 spring and runoff-fed streams in New Zealand and in Northern Spain, respectively. Moss biomass and resource levels were more abundant in springbrooks than in runoff-fed streams. Invertebrate densities were greater in spring habitats, but invertebrate richness was higher and lower at more stable sites in New Zealand and Spain, respectively. These differences in invertebrate diversity may be related to the larger diversity of invertebrate predators in New Zealand springbrooks, and the lack of temperature mediated life history cues in the New Zealand invertebrate fauna. I carried out two experiments to look at the effect of local factors on the spatial distribution of invertebrate communities. The first experiment investigated the effect that algal biomass and habitat structure had on stream invertebrate communities. To do this I used artificial canopies to reduce algal growth and artificial substrates with different habitat complexities. Numbers of invertebrate taxa and individuals were both lower on bricks under the artificial canopies and on the simplest substrates. Algal productivity may enhance invertebrate richness by increasing the number of individuals in a given area, whereas habitat complexity may increase invertebrate richness by providing greater food and/or space resources. The second experiment examined the effects of primary productivity and physical disturbance on stream invertebrates by using artificial canopies and by kicking and raking patches of the stream bed (10 m2). We compared the effects of natural versus experimental disturbance on the benthic invertebrate fauna Invertebrate fauna in high productivity patches recovered quicker than in low productivity patches after both experimental and natural disturbance. The experimental disturbance reduced number of invertebrate taxa and individuals to a greater extent than the spate. Primary productivity limited the recovery of the invertebrate fauna after the disturbances. I also investigated temperature patterns in five runoff and seven spring-fed streams in the North and South Islands of New Zealand. The invertebrate fauna was sampled at 4 distances (0, 100, 500 and 1 km) from seven spring sources. Temperature variability was much larger for runoff-fed streams than for springs, and it increased with distance from the source. Flow, altitude, and the number and type (i.e., spring or runoff-fed) of tributaries joining the springbrook channel determined the degree of temperature variability downstream of the spring sources. Moving downstream, invertebrate communities progressively incorporated taxa with higher mobility and those more common to runoff-fed streams. Changes in substrate composition, stability and invertebrate drift are more likely explanations of the observed longitudinal patterns in the invertebrate communities than changes in temperature regimes.

Species that are introduced outside of their native ranges are an important threat to biodiversity. In southwestern Alberta, Canada, sport and bait fish have been introduced into most waterbodies. I examined the effects of introduced trout and minnows on the distribution, demography and behaviour of larval long-toed salamanders through a combination of field surveys, laboratory experiments and an outdoor mesocosm experiment. Results from field surveys at 30 high-elevation (>1500m) lakes confirmed previous studies showing an allopatic distribution of trout and long-toed salamanders. The same pattern was also documented at 27 low-elevation (<1500m) ponds. In a mesocosm experiment, salamander survival was significantly reduced in ponds containing trout or minnows. Surprisingly, larvae exposed to minnows were 28-65% smaller than larvae in control ponds, suggesting strong interspecific competition for zooplankton prey. In a series of laboratory studies, trout preyed directly on salamander hatchlings and larvae, whereas minnows injured hatchlings but did not consume them. In laboratory aquaria, salamander larvae spent significantly more time within a refuge when exposed to minnow cues, but showed no behavioural response to trout. This confirmed my expectation that long-toed salamanders lack specific behavioural responses to trout, but respond generally to disturbances within the water column. Thus, direct predation and a lack of specific antipredator behaviour are among the likely mechanisms responsible for the observed allopatic distribution of trout and long-toed salamanders. My data also show that gape-limited fish reduce growth and survival of salamanders, perhaps more so than trout, through mechanism such as competition and behavioural alteration. / ix, 76 leaves : ill., map ; 29 cm.

Water samples and macroinvertebrates were collected from three stations of Finley Creek, Indiana before and after a surface cleanup of an adjacent hazardous waste disposal site. Water quality parameters: iron, total dissolved solids, conductance, hardness, total suspended solids and pH were compared between stations and years. The water quality above the landfill was high but decreased downstream. The water below the disposal site contained high levels of chemicals in both studies. However, the concentrations of chemicals were lower in the water two years after the cleanup.Numbers of macroinvertebrates and the number of taxa were determined and species diversity indicies were established. The results showed a statistically significant increase in macroinvertebrate taxa and mean species diversity indicies below the waste disposal site after the cleanup. These results indicate an improvement of water quality. / Department of Natural Resources

Temporary rivers in dryland regions are subject to highly variable and unpredictable rainfall and flow, and are considered unstable systems relative to perennial rivers of temperate zones. Little is known of the ecology of South Africa's temporary rivers, despite their abundance. The research presented focusses on the aquatic invertebrates inhabiting intermittently-flowing rivers in the 'arid corridor' of the Eastern Cape, South Africa. The major aims of the work were: to investigate which invertebrates inhabit these rivers, how the fauna is affected by environmental variability at various spatial and temporal scales, whether or not a characteristic fauna can be associated with these rivers or the hydrological phases which characterise them, and to establish to what extent faunal overlap occurs between different temporary rivers and between natural and modified temporary and perennial rivers. Research results indicate that the fauna of the Eastern Cape temporary rivers shows little structure at the scales of investigation, and that communities are generally non-equilibrial in character. The major forces shaping the biota are considered to be physical rather than biological, and the most important of these are: present and antecedent hydrological conditions and their duration; time of year; and biotope character, extent and heterogeneity. Three groups of fauna were identified as inhabitants of the temporary rivers: 'residents', 'facultative taxa' and 'opportunist colonists'. Taxa were tentatively allocated to these groups. Faunal resemblance was fairly high in Eastern Cape temporary rivers at the level of family, but not at more detailed taxonomic levels. A comparison between faunas of temporary and perennial rivers in different regions of South Africa illustrated the influence of geographical location on diversity. Within regions, however, there was no indication that perennial rivers were more diverse than intermittent rivers. The relatively high diversities in temporary rivers in this study are attributed at least in part to the heterogeneity in surface water conditions over time. A conceptual framework envisioning a continuum between perennial and temporary rivers is proposed as a basis for ongoing research into these rivers. A supporting terminology for the range of river flow types in South Africa is presented.

M.Sc. (Zoology) / The Limpopo Province covers an area of 12.46 million hectares and these accounts for 10.2 per cent of the total land area of the Republic of South Africa. The province is endowed with abundant agricultural resources and it is one of the country’s prime agricultural regions noted for the production of fruits and vegetables, cereals, tea, and sugar. A key feature of the agricultural industry of Limpopo Province is its dualism. There are two distinct types of agricultural production systems. The large scale commercial farming system occupies approximately 70% of the total land area. The smallholder farms are located mostly in the former homeland areas and they cover approximately 30% of the provincial land surface area. The town of Thohoyandou, with its surrounding villages, is the area of greatest human concentration in the Luvuvhu Catchment and subsistence farming is about a third of the total agricultural component. It is important to study the effects of agricultural inputs (e.g. fertilizers and sediment loads) on aquatic ecosystems in order to fully understand the processes involved of these stresses on aquatic ecosystems. Knowledge of these impacts toward the environment and human health is often limited due to lack of capacity building, especially among small scale farmers. Ten bio-monitoring sites were studied on five systems in the Vhembe district. The sites were sampled during the low flow period of November 2011 and the high flow period of April 2012. Sampling sites were selected to present conditions in the Mutale, Mutshindudi and Tshinane Rivers upstream and downstream of the potential influence of small scale agricultural activities...

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