The environmental impact of a transportation system is critical in the assessment of the transportation system performance. Eco-Cooperative Adaptive Cruise Control (Eco-CACC) systems attempt to minimize vehicle fuel consumption and emission levels by controlling vehicle speed and acceleration levels. The majority of previous research efforts developed and applied Eco-CACC systems on either freeway or signalized intersections independently on simple and small transportation networks without consideration of the interaction among these controls. This thesis extends the state-of-the-art in Eco-CACC evaluation by conducting a comprehensive evaluation on a complex network considering Eco-CACC control on both freeways and arterials individually and simultaneously. The goal of this study is to compare Eco-CACCs on arterial facilities (Eco-CACC-A), freeway facilities (Eco-CACC-F) and both facilities (Eco-CACC-F+A). The effects of Eco-CACC are evaluated considering various Measures of Effectiveness (MOEs), including: average vehicle delay, fuel consumption, and emission levels using simulated results from INTEGRATION, a microscopic traffic assignment and simulation software, considering different freeway speed limits, traffic demand levels and system market penetration rates. In total, 19 traffic scenarios for each of the four different cases (Eco-CACC-A, Eco-CACC-F and Eco-CACC-F+A plus a base no control case) were tested. In total 760 simulation runs were conducted (4 cases * 19 scenarios * 10 repetitions). T-tests and pairwise mean comparison (Tukey HSD) were conducted to identify any statistical differences between control cases and the base case from the simulation results. This thesis shows that arterial and freeway Eco-CACCs can work well together and their effects will be largely influenced by network characteristics. / Master of Science