Orientador: Rogerio Gomes / Banca: Silvia Angélica Domingues de Carvalho / Banca: Sebastião Neto Ribeiro Guedes / Resumo: A inovação vem sendo inserida em diversos setores como fator fundamental para reposicionar empresas e países. O presente trabalho propõe um estudo analítico sobre o desenvolvimento de inovação e diferenciação de produto por parte da empresa suíça Nestlé, líder em estrutura e faturamento no mercado mundial da Indústria de Alimentos Processados (IAP), que vem investido desde 2000 no segmento de alimentos funcionais e desde 2011 vem se diversificando em direção a indústria farmacêutica por meio da criação de duas organizações, uma se refere a Nestlé Ciência da Saúde S.A., onde são desenvolvidos produtos voltados à saúde e a outra diz respeito ao Instituto de Ciências da Saúde da Nestlé, no qual são desenvolvidas pesquisas de biotecnologia para analisar DNA humano e assim oferecer produtos personalizados. O objetivo do estudo é investigar os principais fatores que levaram essa gigante em alimentos se diversificar em direção à indústria farmacêutica para promover inovação de produto, traduzidos aqui como alimentos voltados à saúde. Isso significa observar as características e determinantes da inovação da Nestlé, durante as duas últimas décadas, a fim de analisar a existência de uma relação entre a inovação de produto na forma de alimentos funcionais e a tendência dessa grande empresa de alimentos processados em se diversificar em direção à indústria farmacêutica como forma de competição; bem como, identificar o impacto da inovação do produto sobre sua quantidade de vendas, lucros ... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Innovation has been inserted in varioussectors as a key factor to reposition companies and countries. This paper proposes an analytical study about the development of innovation and differentiation of product by the Swiss company Nestlé, leader in structure and sales in the global market for Processed Foods Industry, which since 2000 has invested in the functional food segment and since 2011 has diversified towards the pharmaceutical industry through the creation of two organizations: the first one refers to Nestlé health Science SA, where are developed products aimed at health, and the second one concerns to the health Sciences Institute of Nestle where biotechnology research is designed to analyze human DNA and thus offer customized products. The purpose is to investigate the main factors that led the giant in food diversify toward the pharmaceutical industry to promote product innovation, translated here like food that focus is on health. This means, observing the characteristics and determinants of Nestlé innovation in the last two decades, for the purpose of analyze the existence of a relationship between product innovation in the form of functional foods and the trend of this great food company processed in diversification into toward the pharmaceutical industry as a means of competition; and identify the impact of product innovation on their quantity of sales, profits and market share in the world and in Brazil. Recent literature shows that the life cycle of the Industry Product Processed Food (IAP) has been reduced owing to the introduction of innovations, several focused on differentiation, but some innovations sight to consolidate a new technological path for the industry. / Mestre

Os objetivos desse estudo foram caracterizar modelos experimentais validados para avaliação da depressão em animais; avaliar a viabilidade do desenvolvimento de nanopartículas de cúrcuma a partir do encapsulamento com zeína pelo método de dispersão líquido-liquido; avaliar sua possível atividade antidepressiva em ratos submetidos ao protocolo de estresse crônico moderado e imprevisível (ECMI), considerado modelo preditivo para indução de depressão. No protocolo experimental foram utilizados 40 ratos da linhagem Sprague- Dawley divididos em 5 grupos (n=8 ratos/grupo): Estresse crônico moderado e imprevisível+nanopartículas (ECMI+NANO), Nanopartícula + Controle (NANO), Estresse crônico moderado e imprevisível + Veículo (ECMI), Estresse crônico moderado e imprevisível + fluoxetina (ECMI+FLUOX), Controle + Veículo (CONTROLE) sendo veículo a água filtrada,também utilizada para ressuspender as nanopartículas. A avaliação de anedonia, considerada chave para mensuração do ECMI, confirmou que o protocolo foi efetivo para avaliar esse comportamento. No TNF observou-se que, os grupos estressados apresentaram maiores valores de imobilidade, comportamento similiar ao desamparo aprendido. A administração de nanopartículas (10 mg kg-1) preveniu a elevação dos níveis de corticosterona em ratos submetidos ao protocolo de ECMI. Estes dados sugerem que as nanopartículas de zeína contendo cúrcuma podem contribuir para uma possível ação neuroprotetora deste alimento funcional na depressão Além disso, comprovou-se que o encapsulamento possibilitou a elaboração de nanopartículas com boas características, apresentando alta eficiência de encapsulamento e baixo índice de polidispersão. / The objectives of this study were to characterize validated experimental models for the evaluation of depression in animals; to evaluate the viability of the development of turmeric nanoparticles from the zein encapsulation by the liquid-liquid dispersion method; to evaluate its possible antidepressant activity in rats submitted to the chronic mild and unpredictable stress procedure (CMUS), considered a predictive model for induction of depression. In the experimental protocol, 40 Sprague-Dawley rats were divided into 5 groups (n = 8 rats / group): chronic mild and unpredictable stress procedure + nanoparticles (CMUS + NANO), Nanoparticle + Control (NANO), chronic mild and unpredictable stress procedure + Vehicle (CMUS), chronic mild and unpredictable stress procedure + fluoxetine (CMUS + FLUOX), Control + Vehicle (CONTROL) being a filtered water vehicle, also used to resuspend the nanoparticles. The evaluation of anhedonia, considered key to CMUS measurement, confirmed that the protocol was effective in evaluating this behavior. In TNF, it was observed that the stressed groups showed higher values of immobility, a behavior similar to learned helplessness. The administration of nanoparticles (10 mg kg-1) prevented the elevation of corticosterone levels in rats submitted to the CMUS protocol. These data suggest that turmeric- containing zein nanoparticles may contribute to a possible neuroprotective action of this functional food in depression. In addition, it was verified that the encapsulation allowed the elaboration of nanoparticles with good characteristics, presenting high encapsulation efficiency and low polydispersion index

Orientador: Daniela Cardoso Umbelini Cavallini / Banca: Raquel Bedani / Banca: Valéria de Carvalho Santos Ebinuma / Resumo: A utilização de microrganismos probióticos para a manutenção da saúde bucal tem despertado o interesse da comunidade científica, tendo em vista que estudos indicam que tais microrganismos podem conferir benefícios como: atividade anticariogênica, tratamento de doenças periodentais e halitose, e redução na população de microrganismos patogênicos relacionados a patologias bucais. Nesse sentido, o objetivo do presente trabalho foi o desenvolvimento e a caracterização de uma pastilha alimentícia diet contendo o microrganismo Enterococcus faecium CRL 183 e a avaliação do potencial da referida cepa probiótica em sobreviver em saliva humana e inibir a multiplicação da cepa patogênica Streptococcus mutans ATCC 25175. O microrganismo E. faecium CRL 183 foi microencapsulado por extrusão e coacervação em matriz complexa. A técnica de coacervação complexa apresentou o melhor desempenho em relação à manutenção da viabilidade durante o período de armazenamento à temperatura ambiente (23ºC), sendo selecionada para a produção das pastilhas potencialmente probióticas. As pastilhas foram processadas em três tratamentos: PC - pastilha controle, sem adição do probiótico; PPP1 - com adição do microrganismo probiótico e PPP2 - pastilha com adição do microrganismo probiótico e de inulina (prebiótico). Análises microbiológicas (viabilidade e segurança), físico-químicas (umidade, atividade de água, pH e cor) e sensoriais (testes de aceitação e intenção de compra) foram conduzidas no tempo inicial e ao longo do tempo de estocagem. A sobrevivência do probiótico à saliva e a inibição da multiplicação da cepa patogênica, foram avaliadas através da inoculação da cepa pura e das pastilhas em saliva e da técnica de difusão em poços, respectivamente. A cepa de E. faecium teve sua... / Abstract: The interest in the local effect of probiotic microorganisms is increasing among the scientific community, since studies have indicated that such microorganisms can present anticariogenic activity, help on the treatment of periodontal and halitosis diseases, and reduction in the population of pathogenic microorganisms associated to oral pathologies. The main purpose of this study was the development and characterization of a diet lozenge containing the probiotic strain Enterococcus faecium CRL 183. Its potential of surviving in the human saliva environment and the inhibition of the pathogenetic strain Streptococcus mutans ATCC 25175, were also evaluated. The probiotic strain E. faecium CRL 183 was microencapsulated through the extrusion and complex coacervation techniques. The last one was selected to the next step of the study (lozenges production), as it showed the best performance in comparison to the extrusion technique, regarding viability maintenance during the storage period at room temperature (23ºC). The lozenges were produced through three different treatments: PC - control formulation, without the probiotic; PPP1 - probiotic formulation; PPP2 - probiotic formulation with inulin addition. Microbiologic (viability and security), physicochemical (moisture, water activity, pH and color) and sensorial (acceptance and purchase intention) analyses were conducted during the storage period. The probiotic survival to human saliva was evaluated through inoculation of the pure probiotic strain and the probiotic lozenges in saliva. The agar diffusion technique was used to evaluate the E. faecium CRL 183 inhibition potential against the pathogenic strain, S. mutans ATCC 25175. The probiotic strain had the viability decreased after the lozenges production and also during the storage period at room temperature for the PPP1 and PPP2 ... / Mestre

No description available.

Thesis (Master of Applied Sciences in Chemistry)--Cape Peninsula University of Technology, 2018. / The high demand of omega-3 fish oil nutraceuticals (dietary supplements) is due to the numerous health benefits contributed by the polyunsaturated fatty acids (PUFA’s). The nutraceutical industry is required to follow good manufacturing practice (GMP) standards in order to ensure label claims and prevent adulteration. It is vital that the quality control (QC) procedures will be able to detect adulterated products. It is thus necessary to ensure the analytical techniques are adequate by using validated methods. The qualification or identification of natural fish oils is a difficult task due to overlapping concentration ranges of PUFA’s and other similar properties. Gas chromatography (GC) is the prescribed technique in the nutraceutical industry for analysis of omega-3 fatty acids, but it is time-consuming and costly. Near infrared (NIR) spectroscopy is a rapid and cost-effective technique that can replace the prescribed method if proven equivalent, through validation according to pharmaceutical criteria. In this study, NIR spectroscopy in combination with chemometrics was considered as an alternative method to GC, to identify various commercial fish oils and to quantify the PUFA’s. Identification methods were developed for nine commercial omega-3 fish oils using spectral libraries. Quantitative NIR methods were developed for arachidonic acid (AA), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) in fish oils expressed as mg.g-1 as well as percentage (%) area using partial least squares (PLS) regression and independent validation by superimposing datasets with mutual properties. Based on the statistics in terms of SEC, R2, SEP and r of the PUFA models, the NIR method was equivalent to the prescribed GC methods and precision results obtained were within the prescribed criteria. NIR spectroscopy and chemometrics can be used for conclusive identification and quantification of omega-3 fish oils, thereby minimizing the risk of adulteration. The method also complied with the prescribed pharmaceutical method validation criteria; therefore, was demonstrated as an alternative method to GC for the nutraceutical industry.

Extraction of fructo-oligosaccharide syrups from grated jerusalem artichoke (JA) tubers was studied by water at 20-60&deg / C by determining the yield, degree of polymerization (DP), product profile (DP of up to 6) and prebiotic effect using Lactobacillus plantarum on samples harvested between October and April, stored for 0-20 days. The optimum solvent to solid ratio was 4, the duration of shaking water bath extraction was 40 min and yield based on JA were 12-17%. Temperature was found to improve yield and functionality, and citric acid, at 26 mM, improved the color and darkness by 70 and 80%, respectively. Short-time (1 min) microwaving prior to extraction increased the yield by about 20%, decreased the amount of sugars with DP 1 and 2 and increased the amounts of oligosaccharides (OS) with DP 3-6, although the prebiotic effect increased only slightly / while the color and darkness of the syrup were tripled. Ultrasound-assisted-extraction (USE) gave best performance at 3 min duration / decreased the amounts of sugars with DP 1-2, increased the amounts of OS with DP 3-6, with 18% decrease in the yield. The better functionality of USE syrups were also indicated by 2.5 times higher growth rate of L.plantarum. The application of USE at 60&deg / C compared to 20&deg / C almost tripled the amounts of functional sugars. In order to obtain the largest proportion of monosaccharide units as functional sugars, 10 day storage at 4&deg / C after harvest was indicated. Ultrasonication did not affect the color but the darkness was doubled. The density and viscosity of all the syrups were practically the same.

Thesis (M.S.) in Food Science and Human Nutrition--University of Maine, 2002. / Includes vita. Bibliography: leaves 43-48.

Thesis (Ph.D.)--University of Wollongong, 2005. / Typescript. Includes bibliographical references: leaf 203-253.

Thesis (M.Sc. (Food and Nutrition for Development))--Mahidol University, 2008. / LICL has E-Thesis 0036 ; please contact computer services.

Thesis (PH. D.)--Michigan State University. Food Science, 2008. / Title from PDF t.p. (viewed on Sept. 2, 2009) Includes bibliographical references (p. 181-200). Also issued in print.