Thesis (Ph. D.)--University of Canterbury, 2008. / Typescript (photocopy). "January 2008." Includes bibliographical references (leaves 201-215). Also available via the World Wide Web.

Thesis (Ph. D.)--University of California, San Diego, 1998. / Vita. Includes bibliographical references (p. 169-179).

Thesis (Ph.D.)--Flinders University, Dept. of Chemistry. / Typescript bound. Includes bibliographical references: (p. 360-369) Also available online.

CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / This study aimed to the chemical and pharmacological research of biodiversity of marine fungi associated with sediment from the coastline of northeastern Brazil, especially the state of CearÃ. From the collection of marine sediments at the beach Pecem - SÃo GonÃalo do Amarante-CE, were cultivated various fungi, of which the strain identified as Aspergillus sp. (BRF 087), showed a preliminary cytotoxic activity. The methodology has been directed in finding secondary metabolites with cytotoxic activity using a kinetic fungus study was grown in four different media, BD (potato dextrose), BDL (potato dextrose and yeast), MPD (malt, peptone and dextrose ) and MntPL (mannitol, peptone and yeast) and in different culture periods (7, 14, 21, 28 days). This procedure resulted in the isolation of nine diketopiperazines two tetrapeptides cycle, 3 derivatives of succinic acid and p-hydroxyphenylacetic acid, characterized as cyclo (L-Pro-L-Leu) (A-1), cyclo (L-Pro-L -Phe) (A-2), cyclo (4-OH-Pro-Leu) (A-3), cyclo (4-OH-Pro-Phe) (A-4), cyclo (L-Pro-L-tyr ) (A-5), cyclo (L-Leu-L-Val) (A-6), cyclo (L-Phe-L-Val) (A-7), cyclo (L-Phe-L-Leu) (A-8), cyclo (L-Leu-L-Ile) (A-9), cyclo (L-Ile-L-Pro-L-Leu-L-Pro) (A-10), cyclo (Leu-Ile Leu-Phe) (A-11), 2-metilenosuccinic acid (A-12), 3-Methyl-2-metilenosuccinic (A-13), 4-metoxy-2-methylidene-4-oxobutanoic acid (A-14) and p-hydroxyphenylacetic acid (A-15). The isolation of secondary metabolites was conducted by using usual chromatographic techniques, including chromatography on reverse phase C18 column and high-performance liquid chromatography (HPLC). For structural characterization of the compounds were used customary spectrometric techniques like IR, mass spectrometry and nuclear magnetic resonance (NMR), one and two dimensional, and compared with literature data. / O presente trabalho teve como objetivo principal a investigaÃÃo quÃmico-farmacolÃgica da biodiversidade dos fungos marinhos associados a sedimentos da costa litorÃnea do Nordeste do Brasil, e em especial do estado do CearÃ. A partir da coleta de sedimentos marinhos na praia do PecÃm - SÃo GonÃalo do Amarante-CE, foram cultivados vÃrios fungos, dos quais a cepa identificada como Aspergillus sp. (BRF 087), mostrou uma atividade citotÃxica preliminar. A metodologia empregada foi direcionada na busca de metabÃlitos secundÃrios com atividade citotÃxica, atravÃs de um estudo cinÃtico do fungo que foi cultivado em quatro meios diferentes, BD (batata dextrose), BDL (batata dextrose e levedura), MPD (malte, peptona e dextrose) e MntPL (manitol, peptona e levedura) e em diferentes perÃodos de cultivo (7, 14, 21, 28 dias). Este procedimento resultou no isolamento de nove dicetopiperazinas, dois ciclo tetrapeptÃdeos, 3 derivados do Ãcido succinio e o Ãcido p-hidroxifenilacÃtico, caracterizados como ciclo (L-Pro-L-Leu) (A-1), ciclo (L-Pro-L-Fen) (A-2),ciclo (4-OH-Pro-Leu) (A-3), ciclo (4-OH-Pro-Fen) (A-4), ciclo (L-Pro-L-Tyr) (A-5), ciclo (L-Leu-L-Val) (A-6), ciclo (L-Fen-L-Val) (A-7), ciclo (L-Fen-L-Leu) (A-8), ciclo (L-Leu-L-Ile) (A-9), ciclo (L-Ile-L-Pro-L-Leu-L-Pro) (A-10), ciclo (Leu-Ile-Leu-Fen) (A-11), Ãcido 2-metilenosuccinio (A-12), Ãcido 3-metil-2-metilenosuccinio (A-13), Ãcido 4-metoxi-2-metileno-4-oxobutanÃico (A-14) e o Ãcido p-hidroxifenilacÃtico (A-15). O isolamento dos metabÃlitos secundÃrios foi realizado atravÃs do uso de tÃcnicas cromatogrÃficas usuais, incluindo cromatografia em coluna de fase reversa C18 e cromatografia lÃquida de alta eficiÃncia (CLAE). Para a caracterizaÃÃo estrutural dos compostos foram utilizadas tÃcnicas espectromÃtricas usuais como infravermelho, espectrometria de massa e ressonÃncia magnÃtica nuclear (RMN), uni e bidimensional, alÃm de comparaÃÃo com dados da literatura.