Regeneration and transformation protocols for 'Comet' red raspberry were optimized with the purpose of making the Agrobacterium-mediated gene transfer system efficient for this crop. Adventitious shoot regeneration from leaf discs was improved using explants 10 mm in diameter and transferring to fresh medium at the fourth week of incubation. Additions of liquid medium to solid medium during incubation decreased regeneration and attempts to release the suppressive influence of larger shoots on initials (apical dominance) did not succeed. The presence of claforan did not affect shoot regeneration, but inoculations with Agrobacterium and the presence of kanamycin decreased regeneration moderately or considerably, respectively. The threshold for kanamycin concentration for screening for kanamycin resistant transformed raspberry tissue was 30 to 40 mg l$ sp{-1}.$ The best co-incubation interval between wild-type Agrobacterium and 'Comet' leaf discs ranged from 2 days for highly virulent strains to 3 or more days for moderate to low virulent strains. Among several wild-type strains, C58 was chosen as the most appropriate partially because a disarmed form was commercially available for use as a non-oncogenic vector for transformation of red raspberry. / The binary plasmid pBI121 containing the marker genes NPTII and GUS encoding kanamycin resistance and $ beta$-glucuronidase activity, respectively, was successfully introduced into the Agrobacterium strain LBA4404, which is a disarmed C58 derivative. Transformation of 'Comet' red raspberry was apparently achieved by inoculating leaf disc explants with LBA4404 containing pBI121. The probable integration and expression of the foreign genes into the plant cells were confirmed by screening for kanamycin resistance, GUS assays and Southern blot analyses. This transformation system appears to be effective and may be useful in further studies on red raspberry for both introduction of genes for desirable agronomic traits and basic studies of gene expression.

This thesis proposes two new variations on the genetic algorithm. The first attempts to improve clustering problems by optimizing the structure of a genetic string dynamically during the run of the algorithm. This is done by using a permutation on the allele which is inherited by the next generation. The second is a multiple pool technique which ensures continuing convergence by maintaining unique lineages and merging pools of similar age. These variations will be tested against two well-known graph theory problems, the Traveling Salesman Problem and the Maximum Clique Problem. The results will be analyzed with respect to string rates, child improvement, pool rating resolution, and average string age. / Department of Computer Science

No description available.

Typescript (photocopy) / viii, 198 leaves, [43] leaves of plates : ill. ; 30 cm / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--Dept. of Biochemistry, University of Adelaide, 1983

Bibliography: leaves 133-144 / 144 p., [15] leaves of plates : ill ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 1986

Thesis (Th.M.)--Dallas Theological Seminary, 2005. / Includes bibliographical references (leaves [61-65]).

Thesis (Ph. D.)--University of Hong Kong, 2007. / Also available in print.

Thesis (Ph.D.)--University of Adelaide, Dept. of Paediatrics, 2003. / "16th September 2002." Accompanying CD contains 2 MPEG clips with accompanying text, and a copy in PDF format of: Recovery of airway cystic fibrosis transmembrane conductance regulator function in mice with cystic fibrosis after single-dose lentivirus-mediated gene transfer / M. Limberis ... [et al.], published in Human gene therapy vol. 13 (2002). Bibliography: leaves xxix-li.

Thesis (Ph.D.)--Baylor University, 2008. / Includes bibliographical references (p. 147-188).

Thesis (Ph. D. in Religion)--Vanderbilt University, May 2010. / Title from title screen. Includes bibliographical references.