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Increased releases of cadmium to the aquatic environment have raised concern over the potential for adverse impacts on freshwater organisms in affected aquatic bodies. This thesis explored the responses of a common freshwater amphipod, Gammarus pulex (G. pulex) to sublethal concentrations of cadmium under different environmental conditions and at various stages of the lifestyle. Endpoints studied encompassed molecular, cellular, physiological and behavioural changes, to enable a comprehensive analysis of the effects of the organism. Exposure to sublethal concentrations of cadmium (Cd) (0.001, 0.005 and 0.01 mg Cd L–1) for 7 and 14 days, influenced the percent survival, induced lipid peroxidation and damaged DNA in haemolymph cells of G. pulex. These concentrations also reduced feeding and ventilation rates as well as the motility, in particular of the females, with increasing Cd concentration and time of exposure. Furthermore, Cd accumulation from water in the body tissues of the amphipods was lower than in their food, with increasing Cd concentrations. These results illustrate how concentrations of Cd below the Environmental Quality Standard for European waters can cause sublethal molecular and cellular damage after relatively short periods of exposure. Histopathological effects of Cd on the gills and hepatopancreas (mid-gut gland) were examined using light, scanning and transmission electron microscopy. Multiple effects were observed on the tissues and cell organelles including external alternations in the muscular fibres of the hepatopancreas, lysis of microvilli and morphological changes in mitochondria. Exposure to 0.01 mg Cd L–1 altered the epithelial layer of the gill, causing vacuolation and lysis across the whole gill structure. Mitochondria showed damage to the inner membrane, shortened cristae and swelling, with an increase in apoptosis at concentrations of 0.005 and 0.01 mg Cd L–1. Collectively, these results document the adverse effects of Cd on target organs at concentrations within the range found in freshwater bodies. An investigation of the effects of water hardness on bioaccumulation and toxicity showed that hardness of water inhibited Cd toxicity and protected the juveniles during long term exposures, without affecting growth rate and food consumption. Soft water reduced the rate of survival, growth rate and food consumption during chronic exposure to low Cd concentrations, and facilitated Cd accumulation in the body parts compared with juveniles exposed in hard water. The results show that water quality plays a vital role in reducing or increasing detrimental effects of low Cd concentrations on the early life stage of amphipods which are a source of food for many species in aquatic environments. Exposure to Cd led to an increase in metallothionein concentrations in the amphipods in both hard and soft water. It also caused disruption to ion/osmoregulation, which may represent one mechanism of compensation for the ions lost in the amphipods exposed to Cd in soft water. In conclusion, these results add to the body of evidence describing the sublethal toxicity of Cd, a priority pollutant, to a common freshwater sentinel species. These results are of relevance for future environmental management and remediation approaches, because they provide scientific data to help in assessing, interpreting and understanding the effects of the heavy metal Cd in freshwater environments.

<p>I rinnande vatten, så som bäckar och åar, har Gammarus pulex en viktig ställning i näringsväven. Arten bidrar till att bryta ner organiskt material och göra energin i detta tillgänglig för andra organismer. G. pulex är dessutom en viktig föda för ett stort antal predatorer. Flera studier visar att G. pulex reagerar på olika temperaturer. Tillväxthastighet, livslängd, reproduktion, aktivitet och syreförbrukning är exempel på karaktärer som påverkas av temperaturen. Ingen studie har tidigare behandlat om anläggningen av exokutikula påverkas av temperaturen. En höjd temperatur ökar tillväxthastigheten och detta skulle kunna ge minskade resurser till generering av exokutikula, såkallad ”trade off”. Min studie syftar till att undersöka hur G. pulex tvingas fördela sina resurser mellan tillväxt och exokutikulans tjocklek i förhållande till förändrade temperaturförhållande. Till undersökningen användes 80 djur från två naturliga system med en genomsnittlig temperaturskillnad på 1,9ºC. Säsongsvariation mellan höst och vinter studerades utifrån samma lokaler. Exokutikulans tjocklek mättes och kroppslängden användes som storleksreferens. Statistisk analys gjordes av kvoten exokutikulatjocklek/kroppslängd och ingen signifikant skillnad hittades mellan de olika grupperna. Denna studie visar att ingen ”trade off” finns mellan exokutikulans tjocklek och tillväxt hos G. pulex, varken i de två systemen, eller p.g.a. säsongsvariation, höst och vinter. Detta kan bero på Amfipoders möjligheter att återanvända kalcium för uppbyggnad av en ny exokutikula samt eventuell god tillgång på resurser. Dessutom ger en höjd metabolism vid ökad temperatur ökade möjligheter till en snabb generering av en ny exokutikula. Vidare studier bör göras i både naturliga och artificiella miljöer för att vidare undersöka en eventuell ”trade off” mellan tillväxt och exokutikula.</p>

I rinnande vatten, så som bäckar och åar, har Gammarus pulex en viktig ställning i näringsväven. Arten bidrar till att bryta ner organiskt material och göra energin i detta tillgänglig för andra organismer. G. pulex är dessutom en viktig föda för ett stort antal predatorer. Flera studier visar att G. pulex reagerar på olika temperaturer. Tillväxthastighet, livslängd, reproduktion, aktivitet och syreförbrukning är exempel på karaktärer som påverkas av temperaturen. Ingen studie har tidigare behandlat om anläggningen av exokutikula påverkas av temperaturen. En höjd temperatur ökar tillväxthastigheten och detta skulle kunna ge minskade resurser till generering av exokutikula, såkallad ”trade off”. Min studie syftar till att undersöka hur G. pulex tvingas fördela sina resurser mellan tillväxt och exokutikulans tjocklek i förhållande till förändrade temperaturförhållande. Till undersökningen användes 80 djur från två naturliga system med en genomsnittlig temperaturskillnad på 1,9ºC. Säsongsvariation mellan höst och vinter studerades utifrån samma lokaler. Exokutikulans tjocklek mättes och kroppslängden användes som storleksreferens. Statistisk analys gjordes av kvoten exokutikulatjocklek/kroppslängd och ingen signifikant skillnad hittades mellan de olika grupperna. Denna studie visar att ingen ”trade off” finns mellan exokutikulans tjocklek och tillväxt hos G. pulex, varken i de två systemen, eller p.g.a. säsongsvariation, höst och vinter. Detta kan bero på Amfipoders möjligheter att återanvända kalcium för uppbyggnad av en ny exokutikula samt eventuell god tillgång på resurser. Dessutom ger en höjd metabolism vid ökad temperatur ökade möjligheter till en snabb generering av en ny exokutikula. Vidare studier bör göras i både naturliga och artificiella miljöer för att vidare undersöka en eventuell ”trade off” mellan tillväxt och exokutikula.

The ecology behind evolutionary diversification is a well studied area of research, whereas the effects of evolution on ecosystems get little attention. In line with ecological theory, evolutionary diversification of a species could influence different ecosystem aspects such as food web composition, energy flow, nutrient cycling etc. The main objective of this study was to investigate whether two diverging ecotypes (reed and chara) of Asellus aquaticus differ regarding their role in two aquatic ecosystem processes: decomposition of terrestrial leaves and grazing of periphyton. Their role in ecosystem process as well as treatment effects on fitness, measured as growth and survival, were investigated in a laboratory experiment with various levels of intra-specific competition and inter-specific interactions with the amphipod Gammarus pulex. The isopods were collected from two Swedish lakes: Lake Tåkern and Lake Fardume. These two lakes represent different history of ecotype divergence. The experimental design consisted of 2-L aquaria, each providing elm leaves (Ulmus glabra), oak leaves (Quercus roburleaves) and periphyton as food sources. Ten treatments with five replicates were applied for each lake and the experiment lasted for four weeks. The study showed that there was no significant difference between chara and reed ecotype in their functional role. However, the rate of ecosystem processes per individual decreased in competitive interactions. In high density, decomposition per dry weight consumer was low and total algae biomass was high at the end of four weeks due to intra-specific competition. Moreover, ecosystem processes were lowest in inter-specific competition between Gammarus pulex and each ecotype. Present study also shows that ecotypes from the different lakes, having different history, had different responses to mortality and growth.

Wood ash application (WAA) has been recommended mainly for two reasons; i) to avoid depletion of minerals in the soil due to whole tree harvest in the forestry and ii) to mitigate harmful effects of acidification of soil and surface waters. In conclusion, the effects on terrestrial ecosystems and, especially, tree growth, can be attributed to the properties of the ash, the dose applied and the specific site at which the ash is applied. The research conducted on the effects of WAA on limnological ecosystems is very limited, and the major purpose of the present thesis was to gain knowledge of the effects of wood ash to different freshwater organisms, and the more comprehensive, limnological effects of WAA in the first stream in Bispgården, Sweden. Effects of wood ash solutions on the unicellular alga Euglena gracilis Klebs, the amphipod Gammarus pulex L., and the moss Fontinalis antipyretica Hedw. were investigated under laboratory conditions. Common in all three species was the decline in performance (growth/velocity/respiration/oxygen evolution) when the concentration of wood ash exceeded 5 g/l and no adjustment of pH was done (alkaline solution). In contrast, different movement parameters (motility, upwards swimming and velocity) in E. gracilis (neutral conditions), and increased growth of F. antipyretica with increased concentrations of wood ash indicated that nutrients in the ash was bioavailable for these organisms. There was no evidence of toxic effects on the organisms from metals or other compounds as a result from exposure to wood ash solutions in the present studies. The field study was conducted in a forest area close to Bispgården, about 100 km NW from Sundsvall, Sweden. The catchment area (50 ha) of the stream Fanbergsbäcken was treated with wood ash in September of 2004 (3,000 kg/ha;selfhardened crush-ash). In general, both biological (diatoms) and chemical (pH, alkalinity, and aluminum (Al) measurements) indicators have shown no significant effect on acidification parameters from the addition of wood ash. There was, however, evidence of an increased pH during spring flood, accompanied with a decrease in the frequency of low pH values (&lt;5.6) during spring flood. In addition to this, alkalinity was significantly higher in the period 2005-2006, compared to that of 2003. High concentrations of toxic forms of Al repeatedly occured in the stream Fanbergsbäcken, and the WAA did not affect the frequencies of high concentrations of toxic Al forms (&lt;50 μg/l). Both the moss F. antipyretica and the leaves from Alnus incana displayed increased potassium (K) concentrations, although other nutrients did not increase from WAA. In conclusion, no evidence of WAA being effective in restoring or improving the ecological status of freshwater environments has been established, either in the literature or in the present field study. On the other hand, there were no indications of harmful effects due to WAA, either. However, we still do not know the effects of wood ash on several organisms (predominantly invertebrates) inhabiting small ponds and other, temporary or permanent, freshwater ecosystems. In the context of WAA, these environments and organisms have not attended any attention in the research published to date, and future studies are strongly encouraged.