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1	Sexual dimorphism by measuring the mesiodistal width of the permanent maxillary and mandibular canine in a sample of the South African population in the Northern Suburb of Cape Town. Abdellatif, Abdelhady January 2019 (has links) Magister Scientiae Dentium - MSc(Dent) / Human identification is one of the major responsibilities in the field of Forensic Odontology as it plays a role in identifying deceased individuals using their oral and dental structures. Furthermore, human identification could be a challenging procedure in catastrophic disasters and mass fatality incidents in terms of decomposed and skeletonized human remains. Identification process can be applied using different methods such as fingerprint, DNA and detention. Gender-determination of the victim facilitates reconstruction and rebuilding in the profiling of a medico-legal case investigation. Gender-determination facilitates the procedure up to 50% for positive identification. Anthropology studies have shown that the sexual dimorphism of skeletal remains and teeth can facilitate the identification process. However, teeth are the hardest structures in the human body and virtually immortal as they can withstand diverse circumstances This study aimed to investigate sexual dimorphism of teeth by measuring the mesiodistal width of the maxillary and mandibular permanent canines in a sample of the South African population living in Cape Town. Two hundred orthodontic study models were used, 50 males and 50 females, between 13-30 years of age. Sexual dimorphism Gender-determination Dental identification mesi-odistal dimensions of canines
2	Development of Novel High-Resolution Melting (HRM) Assays for Gender Identification of Caribbean Flamingo (Phoenicopterus ruber ruber) and other Birds Chapman, Alexandra 14 March 2013 (has links) Unambiguous gender identification (ID) is needed to assess parameters in studies of population dynamics, behavior, and evolutionary biology of Caribbean Flamingo (Phoenicopterus ruber ruber) and other birds. Due to its importance for management and conservation, molecular (DNA-based) avian gender ID assays targeting intron-size differences of the Chromosome Helicase ATPase DNA Binding (CHD) gene of males (CHD-Z) and females (CHD-W) have been developed. Male (ZZ) and female (WZ) genotypes are usually scored as size polymorphisms through agarose or acrylamide gels. For certain species, W-specific restriction sites or multiplex polymerase chain-reaction (PCR) involving CHD-W specific primers are needed. These approaches involve a minimum of three steps following DNA isolation: PCR, gel electrophoresis, and photo-documentation, which limit high throughput scoring and automation potential. In here, a short amplicon (SA) High-resolution Melting Analysis (HRMA) assay for avian gender ID is developed. SA-HRMA of an 81-Base Pair (bp) segment differentiates heteroduplex female (WZ) from homoduplex male (ZZ) genotypes by targeting Single-nucleotide Polymorphisms (SNPs) instead of intron-size differences between CHD-Z and CHD-W genes. To demonstrate the utility of the approach, the gender of Caribbean Flamingo (P. ruber ruber) (17 captive from the Dallas Zoo and 359 wild from Ria Lagartos, Yucatan, Mexico) was determined. The assay was also tested on specimens of Lesser Flamingo (P. minor), Chilean Flamingo (P. chilensis), Saddle-billed Stork (Ephippiorhynchus senegalensis), Scarlet Ibis (Eudocimus ruber), White-bellied Stork (Ciconia abdimii), Roseate Spoonbill (Platalea ajaja), Marabou Stork (Leptoptilos crumeniferus), Greater Roadrunner (Geococcyx californianus), and Attwater's Prairie Chicken (Tympanuchus cupido attwateri). Although the orthologous 81 bp segments of Z and W are highly conserved, sequence alignments with 50 avian species across 15 families revealed mismatches affecting one or more nucleotides within the SA-HRMA forward or reverse primers. Most mismatches were located along the CHD-Z gene that may generate heteroduplex curves and thus gender ID errors. For such cases, taxon and species-specific primer sets were designed. The SA-HRMA gender ID assay can be used in studies of avian ecology and behavior, to assess sex-associated demographics and migratory patterns, and as a proxy to determine the health of the flock and the degree by which conservation and captive breeding programs are functioning. endangered birds gender of sexually monomorphic birds sexually monomorphic birds fledglings nestlings DNA test to sex birds Attwater's Prairie Chicken Accipiter species Ría Lagartos Biosphere Reserve Lesser Flamingo Flamingo Chilean Flamingo Phoenicopterus ruber ruber Phoenicopterus Caribbean Flamingo High-resolution Melting Analysis CHD1-W CHD1-Z polymorphism W chromosome Z chromosome CHD1 gene CHD gene gender of birds sex determination of birds gender determination of birds multiple avian species non-invasive conservation of birds sex-ratio of birds sex determination molecular avian gender molecular gender determination Universal sexing of non-ratite birds

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Sexual dimorphism by measuring the mesiodistal width of the permanent maxillary and mandibular canine in a sample of the South African population in the Northern Suburb of Cape Town.

Development of Novel High-Resolution Melting (HRM) Assays for Gender Identification of Caribbean Flamingo (Phoenicopterus ruber ruber) and other Birds