Magister Scientiae Dentium - MSc(Dent) / Human identification is one of the major responsibilities in the field of Forensic Odontology as it plays a role in identifying deceased individuals using their oral and dental structures. Furthermore, human identification could be a challenging procedure in catastrophic disasters and mass fatality incidents in terms of decomposed and skeletonized human remains. Identification process can be applied using different methods such as fingerprint, DNA and detention. Gender-determination of the victim facilitates reconstruction and rebuilding in the profiling of a medico-legal case investigation. Gender-determination facilitates the procedure up to 50% for positive identification. Anthropology studies have shown that the sexual dimorphism of skeletal remains and teeth can facilitate the identification process. However, teeth are the hardest structures in the human body and virtually immortal as they can withstand diverse circumstances This study aimed to investigate sexual dimorphism of teeth by measuring the mesiodistal width of the maxillary and mandibular permanent canines in a sample of the South African population living in Cape Town. Two hundred orthodontic study models were used, 50 males and 50 females, between 13-30 years of age.