Much scientific and media attention has been devoted to the growing body of research into the genetic correlates of human phenomena. However, many of the resulting reports lead to a deterministic interpretation of the role of genes, and involve fundamental misunderstandings of genetics and heredity. Hence, questions arise regarding the ways in which people make sense of the behavioural genetics research they encounter in everyday life. Furthermore, essentialist accounts are often embedded within popular understanding of politically sensitive topics, such as eugenics, race, and sex, and therefore it is important to examine how people comprehend genetic influences on behaviour. In this dissertation, I review current findings regarding the effects of genetic attributions on beliefs, attitudes, and behaviours in the context of the social world. Particular attention is paid to such effects in the context of gender issues. Specifically, in three studies I examine the effects of exposure to scientific theories concerning human sexuality on attitudes towards and evaluations of men’s dubious sexual behaviors. The results indicate that among men exposure to evolutionary psychology arguments leads to more lenient evaluations and judgments of an array of dubious sexual behaviors, compared with exposure to social constructivist arguments. It also seems that men implicitly hold nativist perceptions with regards to male sexuality and promiscuity. The findings were less conclusive among women, with some indication that women are less affected by such exposure as well as less likely to naturally hold a nativist perspective in the context of human sexuality. This empirical research has direct implications for previously suggested intervention programs and adds to the incurrent resurgence of interest in the effects of genetic theories. Finally, I identify areas where further exploration is needed, suggest potential solutions for specific problems, and evaluate related individual and social implications.

No description available.

Prolactin receptor (PRLR) is a membrane anchored protein mediating the biological actions of prolactin. The turkey PRLR (tPRLR) cDNA was isolated and characterized. The open reading frame (ORF) of tPRLR predicted 831 amino acid residues composed of a signal peptide, an extracellular domain (ECD), a single transmembrane domain and an intracellular domain. The deduced amino acid sequence of the turkey prolactin receptor is 53.8%, 51.7%, 49.8%, 49.8%, 80.3% and 89.91% identical to that of the rabbit bovine, human, long form of the rat, pigeon and chicken PRLRs, respectively. The extracellular domain contains two homologous repeat units with 63% amino acid sequence identity to each other. The membrane-distal and membrane-proximal repeats were 53--60% and 62--70% identical to the ECDs of the mammalian PRLRs, respectively. A tPRLR transcript with a molecular size of 3 kilo nucleotides was identified and was detectable in 26 tissues examined. The pituitary gland, crop sac, duodenum and gizzard were found to express the highest levels of tPRLR mRNA among the 26 tissues. In most tissues examined there was no obvious relationship between blood levels of PRL, reproductive states and estimated concentrations of the receptor mRNA. However, in the hypothalamus, increasing blood levels of PRL were associated with decreasing levels of receptor transcripts (p < 0.05), whereas, the opposite relationship was observed in the pituitary gland (p < 0.05). The extracellular domain of tPRLR (tPRLR-ECD) was expressed as a GST fusion protein (tPRLR-ECD-GST) in E. coli. The expression of tPRLR-ECD-GST in BL21 strain yielded a protein with a molecular mass of 76 kDa. About 99% of the fusion protein was present in inclusion bodies and about 50% of the total protein in inclusion bodies was the fusion protein. The insoluble fusion protein was denatured, refolded and purified using GST affinity chromatography. The yield of the purified fusion protein was 20 mg per liter with an estimated p

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No description available.

Nucleotide sequence data from several pea (Piswn sativum L.) seed storage protein genes was obtained. Of two legumin genes sequenced, one was shown to be a pseudogene, apparently once coding for a polypeptide belonging to the 'major' legumin class, whilst the other was shown to be a functional gene coding for a polypeptide of the 'minor' legumin class. Sequence data was also obtained for two vicilin genes. Complete sequencing of one revealed it to be truncated by sequence of unknown origin at its 3' end, whilst partial sequence for the other suggested the presence of a stop codon in the coding region. These findings implied that both vicilin genes are no longer functional. Additionally, various comparisons of nucleic acid and amino acid sequence data were made between these genes and also with other legume seed storage protein genes. Results showed these genes conform with the major structural features of eukaryotic genes, and also revealed the presence of potential tissue -specific regulatory elements in the 5' flanking regions of the genes. Dendrograms for legume 11S and 7S classes of globulin seed storage protein genes clearly supported the model theory of each class having arisen by successive duplications from a common ancestral gene.

Cell patterning during embryogenesis is essential for establishing the identity of the developing body plan. Hox genes are fundamental regulators of tissue organisation along the anterior-posterior body axis of the developing embryo. These homeodomain-containing proteins act as transcription factors during normal development. The function of the homeodomain is to bind sequence-specific DNAmotifs which allows either activation or repression of downstream effector genes, which consequently results in the control of tissue-specific determination and differentiation. Aberrant expression of such Hox genes, including Hoxa-9 can result in homeotic transformations leading to phenotypic malformations and oncogenesis. However the normal function of Hoxa-9 is poorly understood. This study explored the potential role for Hoxa-9 in normal development and differentiation. An in situ hybridisation approach was taken to define the expression of Hoxa-9 in the developing mouse. Hoxa-9 was found to expressed in a temporarily and spatially regulated manner, in particular being detected in the developing cardiac atria, ventricles and cardiac vessels during E9.5-E12 stages of development. The expression of this homeotic gene during in vitro differentiation of embryonic stem cells into cardiomyocytes and haematopoietic cells demonstrated a profile that correlated with the emergence of these cell types. The functioning relationship between Hoxa-9 expression and lineage commitment was Airther explored using over-expression in embryonic stem cells. A potential role for Hoxa-9 in normal development is discussed.

No description available.

The leg A gene from Pisum sativum L. has been extensively characterised and a distinct pattern of developmental and organ-specific gene expression demonstrated. Homology between legumin genes from other species has given some indication of those sequences which may be responsible for the regulation at the level of transcription. This study was designed to provide a functional analysis of the upstream sequences. A number of plasmid vectors containing a maximum of 1.2 kb of upstream sequence from the leg A gene of Pisum sativum I., ligated to the coding region of the nopaline synthase (nos) gene, were constructed. The use of smaller promoter fragments and the insertion of spacer DNA within the promoter region was employed in an effort to localise the regions of 5' flanking sequence which may play a role in tissue specific expression. In a minority of tumours derived from tissue transformed with the vector containing the ' full-length ' leg A promoter, low levels of nopaline were detected, but not with those containing a shorter promoter fragment. Results from the analysis of Seed tissue indicates that 800 bp of the leg A promoter was insufficient to direct tissue-specific expression of the fused nopaline synthase gene in transgenic Nicotiana tabacum, although one individual plant showed a constitutive pattern of nopaline synthesis. However, published results obtained with legumin and other storage protein gene promoters would suggest that this promoter fragment should have been sufficient to confer seed-specific expression. This suggests that there may have been undesirable secondary structures, or small undetected rearrangements, introduced during the construction of the transcriptional fusions between leg A and nos. Alternatively the marker gene may be inadequately sensitive to permit detection of low levels of expression.

Much scientific and media attention has been devoted to the growing body of research into the genetic correlates of human phenomena. However, many of the resulting reports lead to a deterministic interpretation of the role of genes, and involve fundamental misunderstandings of genetics and heredity. Hence, questions arise regarding the ways in which people make sense of the behavioural genetics research they encounter in everyday life. Furthermore, essentialist accounts are often embedded within popular understanding of politically sensitive topics, such as eugenics, race, and sex, and therefore it is important to examine how people comprehend genetic influences on behaviour. In this dissertation, I review current findings regarding the effects of genetic attributions on beliefs, attitudes, and behaviours in the context of the social world. Particular attention is paid to such effects in the context of gender issues. Specifically, in three studies I examine the effects of exposure to scientific theories concerning human sexuality on attitudes towards and evaluations of men’s dubious sexual behaviors. The results indicate that among men exposure to evolutionary psychology arguments leads to more lenient evaluations and judgments of an array of dubious sexual behaviors, compared with exposure to social constructivist arguments. It also seems that men implicitly hold nativist perceptions with regards to male sexuality and promiscuity. The findings were less conclusive among women, with some indication that women are less affected by such exposure as well as less likely to naturally hold a nativist perspective in the context of human sexuality. This empirical research has direct implications for previously suggested intervention programs and adds to the incurrent resurgence of interest in the effects of genetic theories. Finally, I identify areas where further exploration is needed, suggest potential solutions for specific problems, and evaluate related individual and social implications.