Diss. (sammanfattning) Uppsala : Univ., 2001. / Härtill 4 uppsatser.

Wong Pak Cheung Ronald. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 121-142). / Abstracts in English and Chinese. / Acknowledgement --- p.I / Abstract --- p.II / Abbreviation --- p.VI / List of figures --- p.IX / List of tables --- p.XI / Content --- p.XII / Content / Chapter Chapter 1: --- General Introduction --- p.1 / Chapter 1.1 --- The p53 tumor suppressor gene --- p.2 / Chapter 1.1.1 --- Structure and function --- p.2 / Chapter 1.1.2 --- Regulation of p53 stability and activity --- p.3 / Chapter 1.1.3 --- p53 and cell cycle arrest --- p.4 / Chapter 1.1.4 --- p53 and apoptosis --- p.4 / Chapter 1.2 --- Mutation in p53 gene --- p.9 / Chapter 1.2.1 --- Loss of function through dominant negative effect --- p.9 / Chapter 1.2.2 --- Gain-of-function through transactivation by mutant p53 --- p.10 / Chapter 1.2.3 --- Mutation in p53 and resistance to cancer therapy --- p.10 / Chapter 1.3 --- Objective of the study --- p.14 / Chapter Chapter 2: --- Mutant p53 induced interferon resistance and its regulation of the Jak/Stat pathway --- p.15 / Chapter 2.1 --- Introduction --- p.16 / Chapter 2.1.1 --- IFN classification and biological activities --- p.16 / Chapter 2.1.2 --- IFN signaling --- p.17 / Chapter 2.1.3 --- IFN direct antitumor effect: cell cycle arrest and apoptosis --- p.18 / Chapter 2.1.4 --- IFN in immunotherapy --- p.20 / Chapter 2.1.5 --- Resistance to IFN therapy --- p.21 / Chapter 2.2 --- Materials and Methods --- p.24 / Chapter 2.2.1 --- Cell lines --- p.24 / Chapter 2.2.2 --- Drugs and antibodies --- p.24 / Chapter 2.2.3 --- Cell Proliferation assay- MTT assay --- p.24 / Chapter 2.2.4 --- Cell cycle analysis --- p.25 / Chapter 2.2.5 --- DNA fragmentation assay --- p.25 / Chapter 2.2.6 --- Western blot analysis --- p.26 / Chapter 2.2.7 --- "Combined treatment of IFNs and Jak inhibitors in MTT assay, DNA fragmentation assay and Western blot analysis" --- p.26 / Chapter 2.3 --- Results --- p.27 / Chapter 2.3.1 --- Mutant p53-V143A and p53-R273H induced IFN resistance: the role of IFN induced apoptosis and cell cycle arrest --- p.27 / Chapter 2.3.2 --- IFN induction of apoptosis: a p53-independent and caspase-dependent pathway --- p.28 / Chapter 2.3.3 --- Mutant p53 regulation of Jak/Stat pathway --- p.36 / Chapter 2.3.4 --- Janus kinases (Jaks) and IFN-alpha sensitivity in Saos-2 cells --- p.41 / Chapter 2.3.5 --- Janus kinases (Jaks) and IFN-gamma sensitivity --- p.49 / Chapter 2.4 --- Discussion --- p.56 / Chapter 2.4.1 --- Mutant p53-V143 and p53-R273H induced IFN resistance in Saos-2 cells --- p.56 / Chapter 2.4.2 --- Role of Jaks in IFN sensitivity in Saos-2 cells --- p.57 / Chapter 2.4.3 --- IFN signaling in Saos-2 cells --- p.57 / Chapter 2.4.4 --- Jak2 and IFN induced apoptosis --- p.58 / Chapter Chapter 3: --- Mutant p53 induced drug resistance --- p.60 / Chapter 3.1 --- Introduction --- p.61 / Chapter 3.1.1 --- The multidrug resistance (MDR) --- p.61 / Chapter 3.1.2 --- Anticancer drugs used in the study: action mechanisms and resistance --- p.67 / Chapter 3.1.3 --- Jak/Stat pathway and MDR --- p.68 / Chapter 3 .2 --- Materials and Methods --- p.72 / Chapter 3.2.1 --- Cell lines --- p.72 / Chapter 3.2.2 --- Drugs and antibodies --- p.72 / Chapter 3.2.3 --- Caspase 3 activity assay --- p.72 / Chapter 3.2.4 --- Cell Proliferation assay- MTT assay --- p.73 / Chapter 3.2.5 --- Cell cycle analysis --- p.73 / Chapter 3.2.6 --- DNA fragmentation assay --- p.73 / Chapter 3.2.7 --- Reverse transcription polymerase chain reaction --- p.73 / Chapter 3.2.8 --- Western blot analysis --- p.74 / Chapter 3.2.9 --- "Combined treatment of IFNs and Jak inhibitors in MTT assay, DNA fragmentation assay and Western blot analysis" --- p.74 / Chapter 3.3 --- Results --- p.75 / Chapter 3.3.1 --- Mutant p53 and drug sensitivity --- p.75 / Chapter 3.3.2 --- Mutant p53 and drug induced apoptosis and cell cycle arrest --- p.75 / Chapter 3.3.3 --- Classical drug resistance factors in mutant p53 induced drug resistance --- p.87 / Chapter 3.3.4 --- The role of Jaks in drug sensitivity of Saos-2 cells --- p.89 / Chapter 3.3.5 --- The role of Jaks in drug induced DNA fragmentationin Saos-2 cells --- p.89 / Chapter 3.3.6 --- Jak signaling and caspase activation in MTX induced apoptosis in Saos-2 cells --- p.100 / Chapter 3.3 --- Discussion --- p.108 / Chapter 3.3.1 --- Mutant p53-V143A and p53-R273H induced drug resistance in Saos-2 cells --- p.108 / Chapter 3.3.2 --- Role of Jaks in drug sensitivity in Saos-2 cells --- p.109 / Chapter 3.3.3 --- Jak/Stat signaling in Saos-2 cells --- p.109 / Chapter 3.3.4 --- Jak2 and MTX induced apoptosis --- p.110 / Chapter Chapter 4: --- General discussion --- p.112 / Chapter 4.1 --- Mutant p53 induced immunotherapy and chemotherapy resistance --- p.113 / Chapter 4.2 --- Gain of new function of mutant p53-V143A and p53-R273H in regulating Jak/Stat pathway leading to resistance to IFN and chemotherapeutic drugs --- p.114 / Chapter 4.3 --- The role of Jaks in MTX sensitivity --- p.114 / Chapter 4.4 --- Future work --- p.115 / Chapter 4.5 --- Perspective --- p.120 / References --- p.121

Thesis (M.Sc.). / Written for the Dept. of Plant Science. Title from title page of PDF (viewed 2008/05/29). Includes bibliographical references.

Thesis (Ph. D.)--University of Chicago, Committee on Developmental Biology, March 2003. / Includes bibliographical references. Also available on the Internet.

by Tsang Wing Pui. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (p. 195-215). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

by Lam, Sau Hing. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references (leaves 141-158). / Abstract also in Chinese. / Content / Acknowledgements / Abbreviation / Project Objectives / Abstract / Chapter Chapter One --- Introduction / Chapter 1.1 --- The definition of homeobox genes / Chapter 1.2 --- Homeobox genes as a transcription factor / Chapter 1.3 --- Homeobox in Drosophila / Chapter 1.3.1 --- The development of Drosophila / Chapter 1.3.2 --- Maternal genes / Chapter 1.3.3 --- Segmentation genes / Chapter 1.3.4 --- Homeobox genes / Chapter 1.4 --- Family of Hox genes in the mammalian system / Chapter 1.5 --- Some possible chemical mechanism in the cascade system of the Hox genes in the vertebrate / Chapter 1.6 --- Hox (Antp-Class homeobox gene) in mammal / Chapter 1.6.1 --- Labial Like homeobox genes / Chapter 1.6.2 --- Proboscipedia Like homeobox genes / Chapter 1.7 --- Divergent homeobox genes / Chapter 1.7.1 --- Paired (prd) Class / Chapter 1.7.2 --- Even-Skipped (Eve) Class / Chapter 1.7.3 --- Distal-less (Dll) Class / Chapter 1.7.4 --- Muscle-Specific Homeobox (Msx) Class / Chapter 1.8 --- Orphan homeobox gene / Chapter 1.8.1 --- The characteristic of Hox 11 sequence in human and mouse / Chapter 1.8.2 --- Novel homeobox genes related to hox 11 gene family / Chapter 1.8.3 --- The mechanism of HOX 11 inducing gene regulation and signal transduction pathways / Chapter 1.8.4 --- HOX 11 in human / Chapter 1.8.5 --- Hox11 in mouse / Chapter 1.8.6 --- Hox11 L1 in mouse / Chapter 1.9 --- Homeobox gene involved in haematopoiesis / Chapter 1.10 --- Some translocations of homeobox genes in blood lineage / Chapter 1.11 --- The development of mouse / Chapter 1.11.1 --- Early organogenesis / Chapter 1.11.2 --- Nervous system development / Chapter 1.11.3 --- Somite development / Chapter 1.11.4 --- Eye development / Chapter 1.11.5 --- Neural crest cell migration / Chapter 1.11.6 --- Branchial arches development / Chapter Chapter Two --- Materials and methods / Chapter 2.1 --- Mouse Embryos / Chapter 2.2 --- RNA extraction / Chapter 2.3 --- Large plasmid preparation / Chapter 2.4 --- The synthesis of cDNAs using Reverse Transcription / Polymerase Chain Reaction (RT-PCR) and ligation into Bluescript® II KS / Chapter 2.4.1 --- The synthesis of RT-PCR products / Chapter 2.4.2 --- The formation of blunt ends of cDNA / Chapter 2.4.3 --- The ligation of cDNA with plasmid vectors / Chapter 2.4.4 --- Transformation / Chapter 2.4.5 --- The miniprep plasmid purification / Chapter 2.5 --- T7 sequencing / Chapter 2.6 --- Double stranded DNA cycle sequencing of plasmid / Chapter 2.6.1 --- Gel electrophoresis / Chapter 2.7 --- Northern blot / Chapter 2.7.1 --- Preparation of Northern blot / Chapter 2.7.2 --- Hybridization of Northern blot / Chapter 2.8 --- DIG labeled probes in whole mount in situ hybridization / Chapter 2.8.1. --- Preparation of linear DNA to generate riboprobes / Chapter 2.8.2 --- Preparation of DIG labeled riboprobe / Chapter 2.8.3. --- Preparation of embryo powder / Chapter 2.8.4 --- Absorption of antibody / Chapter 2.8.5 --- Embryo preparation / Chapter 2.8.6 --- Embryos staining / Chapter 2.9 --- Sections from whole mount in situ hybridization / Chapter 2.10 --- The radiolabeled section in situ hybridization / Chapter 2.10.1 --- The preparation of paraffin wax block and sample sections / Chapter 2.10.2 --- Slide pretreatment / Chapter 2.10.3 --- Preparation of probe / Chapter 2.10.4 --- Hybridization / Chapter 2.10.5 --- Washing / Chapter 2.10.6 --- Emulsification and development / Chapter 2.11 --- DIG-label in situ hybridization of frozen / Chapter 2.12 --- H&E staining / Chapter Chapter Three --- Results and Discussion / Chapter 3.1 --- Synthesis of Tlx3 probes for use in the section and whole mount in situ hybridization / Chapter 3.1.1 --- Synthesis of the Tlx cDNA by RT-PCR method / Chapter 3.1.2 --- The Tlx3 genomic clone for detecting the developmental expression of Tlx3 by Northern Blot / Chapter 3.1.3 --- The characterization of Tlx3 cDNAs and the sonic hedgehog cDNA / Chapter 3.2 --- Section in situ hybridization using different probes / Chapter 3.2.1 --- Section in situ hybridization using the transcribed riboprobes of Pax2 cDNA / Chapter 3.2.2 --- The transcribed riboprobes of Tlx genomic clones were used to hybridize the section in situ hybridization / Chapter 3.2.3 --- The in situ hybridization of frozen sections of mouse embryos using the transcribed riboprobes from Tlx3 cDNA subclone / Chapter 3.3 --- Expression pattern of Tlx3 on whole mount embryos using both cDNA and genomic probes / Chapter 3.3.1 --- The expression of Tlx3 on whole mount in situ hybridization of the mouse embryos using the antisense probes from the Tlx3 genomic clone / Chapter 3.3.2 --- Whole mount in situ hybridization of mouse embryo using the transcribed riboprobes of Tlx3 cDNA / Chapter 3.3.3 --- The expression pattern of sonic hedgehog on embryos at 8.5 to9.5 dpc / Conclusion / Future prospective / Appendix / Reference / Acknowledgement

by Poon Hong Kit. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references (leaves 129-161). / Abstract also in Chinese. / ACKNOWLEDGEMENTS --- p.i / ABSTRACT (ENGLISH/CHINESE) --- p.ii / ABBREVIATIONS --- p.vi / LIST OF TABLES --- p.vii / LIST OF FIGURES --- p.viii / TABLE OF CONTENTS --- p.x / INTRODUCTION --- p.1 / LITERATURE REVIEW --- p.4 / Chapter I. --- Models for studying photoreceptor cell death --- p.4 / Chapter II. --- Photic retinopathy --- p.5 / Chapter II.1. --- Proposed Mechanisms of Photic Retinopathy --- p.5 / Chapter II.2. --- Factors Affecting Photic Retinopathy --- p.6 / Chapter II.3. --- Pathologic Processes of Photic Retinopathy --- p.7 / Chapter III. --- P53 --- p.9 / Chapter III.1. --- Historical Perspective --- p.9 / Chapter III.2. --- Structure of p53 --- p.10 / Chapter III.2.1. --- The p53 Gene & mRNA --- p.10 / Chapter III.2.2. --- The p53 Protein --- p.10 / Chapter III.3. --- Modifications of p53 Protein --- p.13 / Chapter III.4. --- Functions of p53 --- p.14 / Chapter III.4.1. --- p53 & Tumors --- p.14 / Chapter III.4.2. --- p53 & DNA Damage --- p.15 / Chapter III.4.3. --- p53 & Apoptosis --- p.16 / Chapter III.4.3.1. --- p53-dependent Apoptosis --- p.16 / Chapter III.4.3.2. --- p53-independent Apoptosis --- p.20 / Chapter IV. --- clusterin --- p.22 / Chapter IV. 1. --- Historical Perspective --- p.22 / Chapter IV.2. --- Structure of Clusterin --- p.22 / Chapter IV.2.1. --- Clusterin Gene & mRNA --- p.22 / Chapter IV.2.2. --- Clusterin Protein --- p.22 / Chapter IV.3. --- Functions of Clusterin --- p.22 / Chapter IV.3.1. --- Clusterin & Apoptosis --- p.23 / OBJECTIVES --- p.24 / MATERIALS AND METHODS --- p.26 / Chapter V. --- Sample collections --- p.26 / Chapter V.l. --- Induction of Photic Injury in Rat --- p.26 / Chapter V.2. --- Tissue Processing --- p.26 / Chapter V.2.1. --- Paraffin-embedded Tissues --- p.26 / Chapter V.2.2. --- Epoxy-embedded Tissues --- p.27 / Chapter V.3. --- Cell Culture --- p.28 / Chapter VI. --- Light-microscopic study --- p.29 / Chapter VI. 1. --- Histopathology --- p.29 / Chapter VI.1.1. --- Toluidine Blue --- p.29 / Chapter VI. 1.2. --- Morphometry of the ONL Thickness --- p.29 / Chapter VI.2. --- In situ TUNEL --- p.29 / Chapter VI.2.1. --- Morphometry of TUNEL --- p.29 / Chapter VI.3. --- Immunohistochemistry --- p.30 / Chapter VI.3.1. --- Single-labeling --- p.30 / Chapter VI.3.1.1. --- Immunolabeling of p53 Protein --- p.31 / Chapter VI.3.1.2. --- Immunolabeling of p21 Protein --- p.31 / Chapter VI.3.1.3. --- Immunolabeling of bax Protein --- p.31 / Chapter VI.3.1.4. --- Immunolabeling of c-fos Protein --- p.32 / Chapter VI.3.1.5 --- Immunolabeling of Clusterin Protein --- p.32 / Chapter VI.3.2. --- Double-labeling --- p.32 / Chapter VI.3.2.1. --- TUNEL & Fluorescent-labeling of p53 --- p.32 / Chapter VI.3.2.2. --- TUNEL & Fluorescent-labeling of p21 --- p.33 / Chapter VI.3.3. --- Grading of Immunoreactivities --- p.33 / Chapter VI.3.4. --- Morphometry of c-fos Immuno-positive Nuclei --- p.33 / Chapter VI.4. --- In situ RT-PCR --- p.34 / Chapter VI.4.1. --- Isolation of Retinal DNA --- p.34 / Chapter VI.4.2. --- Polymerase Chain Reaction (PCR) --- p.34 / Chapter VI.4.3. --- In situ Reverse Transcriptase 226}0ؤ PCR (RT-PCR) --- p.35 / RESULTS --- p.37 / Chapter VII. --- LIGHT-MICROSCOPY --- p.37 / Chapter VII.1. --- Histopathology & Morphometry --- p.37 / Chapter VII. 1.1. --- Histopathology --- p.37 / Chapter VII.1.2. --- Morphometry of the ONL Thickness --- p.49 / Chapter VII.2. --- DNA Nicks Detection --- p.42 / Chapter VII.2.1. --- In situ TUNEL --- p.42 / Chapter VII.2.2. --- Morphometry of TUNEL --- p.42 / Chapter VII.3. --- p53 --- p.51 / Chapter VII.3.1. --- Immunohistochemistry of p53 --- p.51 / Chapter VII.3.2. --- Grading of p53 Immunoreactivities --- p.51 / Chapter VII.3.3. --- p53 in situ RT-PCR --- p.51 / Chapter VII.3.4. --- Double-labeling ofp53 with in situ TUNEL --- p.59 / Chapter VII.4. --- p21 --- p.74 / Chapter VII.4.1. --- Immunohistochemistry of p21 --- p.74 / Chapter VII.4.2. --- Grading of p21 Immunoreactivities --- p.74 / Chapter VII.4.3. --- Double-labeling of p21 with in situ TUNEL --- p.74 / Chapter VII.5. --- bax --- p.84 / Chapter VII.5.1. --- Immunohistochemistry of bax --- p.84 / Chapter VII.5.2. --- Grading of bax Immunoreactivities --- p.84 / Chapter VII.5.3. --- bax in situ RT-PCR --- p.84 / Chapter VII.6. --- c-fos --- p.96 / Chapter VII.6.1. --- Immunohistochemistry of c-fos --- p.96 / Chapter VII.6.2. --- Morphometry of c-fos immuno-positive nuclei & TUNEL --- p.96 / Chapter VII.7. --- Clusterin / Chapter VII.7.1. --- Immunohistochemistry of Clusterin --- p.108 / Chapter VII.7.2. --- Grading of Clusterin Immunoreactivities --- p.108 / discussion --- p.116 / Chapter VIII. 1. --- Summary --- p.116 / Chapter VIII.1.1. --- Histopathology & Morphometry --- p.118 / Chapter VIII.1.2. --- In situ TUNEL --- p.119 / Chapter VIII.1.3. --- p53 & c-fos --- p.119 / Chapter VIII.1.4. --- p27 --- p.124 / Chapter VIII.1.5. --- bax --- p.124 / Chapter VIII. 1.6. --- Clusterin --- p.124 / Chapter VIII.2. --- Hypothesis --- p.125 / Chapter VIII.3. --- Conclusion --- p.127 / bibliography --- p.129

Thesis (M.Sc.)--University of Alberta, 2009. / A thesis submitted to the Faculty of Graduate Studies and Research in partial fulfillment of the requirements for the degree of Master of Science in Experimental Oncology, Department of Oncology. Title from pdf file main screen (viewed on July 30, 2009). Includes bibliographical references.

Chin Wai. / Original paper published on European Jouranl of cancer (vol. 34, no. 7, p. 1015-1022) inserted. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (leaves 70-77). / Abstracts in English and Chinese. / Dedication --- p.i / Vita --- p.ii / Acknowledgment --- p.iv / Abstract --- p.vii / Introduction --- p.1 / Hepatocellular carcinoma --- p.1 / Lung cancer --- p.3 / The role of suppressor gene PML in cancer --- p.5 / Principle of immunohistaining methods --- p.8 / Patients and methods --- p.21 / Patients and smaples --- p.21 / Slide preparing --- p.22 / Immunohistochemical staining --- p.23 / Cell culture --- p.30 / Determination of the population doubling times --- p.30 / Mtt assay --- p.35 / Results --- p.37 / "Altered expression of PML in normal liver, HCC and Secondary liver tumor" --- p.37 / Increased expression of PML in chronic hepatitis tissues --- p.38 / Differential expression of PML at the periphery and at the center of single-encapsulated lesion of HCC --- p.42 / Expression of PML in normal lung tissues --- p.43 / Suppression of PML expression in small cell lung cancer --- p.44 / Enhanced expression of PML in adenocarcinoma of the lung --- p.44 / Enhanced expression of PML in squamous cell carcinoma of the lung --- p.45 / Express of PML in metastatic lung cancer --- p.46 / Inverse correlation of the expression of PML and the proliferation marker Ki-67 in SCLC and SCC --- p.46 / Correlation of the expression of PML in macrophages with the macrophage-specific marker KP-1 --- p.47 / Expression of PML in Hela cells and Hela cells transfected with the gene --- p.48 / Altered morphology of the Hela-PML cell-clones --- p.49 / Altered growth rate in Hela-PML cells --- p.49 / Altered rate of cell-death in Hela-PML cells --- p.50 / Discussion --- p.51 / Further studies --- p.63 / References --- p.70 / Table --- p.78 / Figure legend --- p.81 / Appendix: Original paper published on European Journal of cancer --- p.106

by Chan Ka Lee. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (leaves 94-112). / Abstracts in English and Chinese. / Acknowledgements --- p.i / abstract(english/chinese) --- p.iii / Contents --- p.vii / List of Tables --- p.xi / List of Figures --- p.xii / Chapter I --- Introduction --- p.1 / Chapter I.1 --- Gastric Lymphoma --- p.1 / Chapter I.1.1 --- Background --- p.1 / Chapter I.1.2 --- Mucosa-Associated Lymphoid Tissue --- p.2 / Chapter I.1.3 --- Classification of Primary Gastric Lymphomas --- p.3 / Chapter I.1.3.1 --- Mucosa-Associated Lymphooid Tissue Type Lymphomas --- p.3 / Chapter I.1.3.2 --- High Grade Primary Gastric Lymphomas --- p.5 / Chapter I.1.3.3 --- Other Gastric Lymphomas --- p.7 / Chapter I.2 --- Helicobater Pylori --- p.8 / Chapter I.3 --- Epstein-Barr Virus --- p.9 / Chapter I.3.1 --- Epidemiology --- p.9 / Chapter I.3.2 --- Virus and Genome Structure --- p.9 / Chapter I.3.3 --- Latent Infection --- p.11 / Chapter I.3.4 --- Latent Membrane Protein-1 --- p.12 / Chapter I.3.5 --- "EBV-Encoded, Small Non-polydenylated RNAs (EBERs)" --- p.13 / Chapter I.3.6 --- Disease Associated with EBV --- p.13 / Chapter I.3.7 --- EBV and PGL --- p.14 / Chapter I.4 --- Genetic Alterations --- p.15 / Chapter I.4.1 --- Background --- p.15 / Chapter I.4.2 --- Tumor Suppressor Genes (TSGs) --- p.16 / Chapter I.4.2.1 --- Origin and Structure of p53 Gene and Protein --- p.16 / Chapter I.4.2.2 --- Functions of p53 Gene --- p.18 / Chapter I.4.2.3 --- Inactivation Mechanisms of p53 --- p.21 / Chapter I.4.2.4 --- p53 Mutations and Protein Expression in NHLs --- p.24 / Chapter I.4.3 --- Oncogene --- p.25 / Chapter I.4.3.1 --- Bcl-2 --- p.25 / Chapter I.4.3.2 --- Other Oncogenes --- p.27 / Chapter II --- OBJECTIVES OF STUD Y --- p.30 / Chapter III --- ma terials and methods --- p.31 / Chapter III.1 --- Materials --- p.31 / Chapter III.2 --- Detection of EB V Latent Gene Product by In-situ Hybridization --- p.33 / Chapter III.2.1 --- Pretreatment of Paraffin-embedded Tissues and Apparatus --- p.33 / Chapter III.2.2 --- In-situ Hybridization of EBERs --- p.34 / Chapter III.3 --- Detection of p53 and bcl-2 and LMP-1 Protein Expression by Immunohisiochemistry --- p.35 / Chapter III.4 --- Microdissection of Formalin-fixed Paraffin-embedded Tissues --- p.37 / Chapter III.5 --- Extraction of Genomic DNA from Formalin-fixed Paraffin-embedded Tissues --- p.38 / Chapter III.5.1 --- Phenol / Chloroform Extraction --- p.38 / Chapter III.5.2 --- Commercially Available DNA Extraction Kit --- p.40 / Chapter III.6 --- Mutational Analysis p53 --- p.41 / Chapter III.6.1 --- Polymerase Chain Reaction - Single Strand Conformation Polymorphism (PCR-SSCP) Analysis --- p.41 / Chapter III.6.1.1 --- PCR primers --- p.41 / Chapter III.6.1.2 --- PCR Amplification ofp53 gene --- p.42 / Chapter III.6.1.3 --- Non-denaturing Polyacrylamide Gel Electrophoresis --- p.42 / Chapter III.6.2 --- DNA Sequencing Analysis --- p.44 / Chapter III.6.2.1 --- Purification of DNA from Shifts on Non-denaturing Gels --- p.44 / Chapter III.6.2.2 --- 5' end Labeling of Primer --- p.45 / Chapter III.6.2.3 --- Cycle Sequencing --- p.45 / Chapter III.6.2.4 --- Denaturing Gel Electrophoresis --- p.46 / Chapter III.7 --- Loss of Heterozygosity (LOH) Analysis on Chromosome 17p --- p.47 / Chapter III.7.1 --- Microsatellite Markers --- p.49 / Chapter III.7.2 --- PCR Amplification of DNA Fragments Containing Polymorphic Microsatellites --- p.49 / Chapter III.7.3 --- Denaturing Polyacrylamide Gel Electrophoresis --- p.50 / Chapter III.7.4 --- Determination of Allelic Abnormalities --- p.51 / Chapter III. 8 --- Statistical Analysis --- p.52 / Chapter IV --- results --- p.53 / Chapter IV.1 --- Association with Helicobactor Pylori (HP) --- p.53 / Chapter IV.2 --- Detection of EBERs by ISH --- p.53 / Chapter IV.3 --- Immunohistochemical Analysis --- p.54 / Chapter IV.3.1 --- Protein Expression of EBV LMP-1 --- p.54 / Chapter IV.3.2 --- Protein Expression of p53 --- p.54 / Chapter IV.3.3 --- Protein Expression of bcl-2 --- p.55 / Chapter IV.3.4 --- Correlation between p53 and bcl-2 Protein Expression --- p.55 / Chapter IV.4 --- Mutational Analysis of p53 --- p.56 / Chapter IV.5 --- LOH Analysis on Chromosome 17p --- p.57 / Chapter V --- DISCUSSION --- p.58 / Chapter V.1 --- Helicobactor Pylori Association --- p.58 / Chapter V.2 --- Association with EE V --- p.60 / Chapter V.3 --- Protein Expression of p53 and bcl-2 --- p.61 / Chapter V.3.1 --- p53 --- p.61 / Chapter V.3.2 --- Bcl-2 --- p.62 / Chapter V.3.3 --- Correlation between p53 and bcl-2 Expression --- p.63 / Chapter V.4 --- p53 Gene Molecular Analysis --- p.65 / Chapter V.5 --- Distribution of Mutations and Molecular Fingerprinting --- p.67 / Chapter V.6 --- Possible Role of p53 Mutation in EBV+ Gastric Lymphomas --- p.69 / ILLUSTRATIONS --- p.71 / references --- p.94