This thesis constitutes the first full-length study of Polish Communists in Canada, a group that provided a substantial segment of the countries socialist left in the early 20th century. It traces the roots of socialist support in Poland, its transplantation to Canada, the challenges it faced within an ethnic community heavily influenced by Catholicism, the complications caused by its links to the Comintern, and its changing strength and decline. It offers a deeper understanding of the ways in which the Communist party was able to appeal to certain ethnic groups, such as through cultural outreach, as well as its complicated and often arguably counter-productive relationship with the Comintern. It also furnishes important information on the efforts of the RCMP and Polish consulates to maintain control over the communists, as well as how generally improved material conditions among Poles, especially following the Second World War, along with the influence of the Cold War, accounted for a rapid decline in support. The thesis is primarily based on sources generated by the Polish Ministry of Foreign Affairs or, more precisely, by the Polish consulates in Winnipeg, Montreal and Ottawa. One the Canadian side, the thesis took advantage of RCMP records, Canadian security bulletins, immigration records and Polish-language newspapers printed in Canada. By utilizing these sources, this study not only analyses the interaction of the Polish Canadian communist movement with other segments of the Polish community in Canada, but it also moves beyond the introverted approach that has characterized most studies of ethnic organizations in Canada by placing the movement within a “Canadian” context to analyze its relations with the government, broader segments of Canadian society, and the Communist Party of Canada (CPC).

This thesis constitutes the first full-length study of Polish Communists in Canada, a group that provided a substantial segment of the countries socialist left in the early 20th century. It traces the roots of socialist support in Poland, its transplantation to Canada, the challenges it faced within an ethnic community heavily influenced by Catholicism, the complications caused by its links to the Comintern, and its changing strength and decline. It offers a deeper understanding of the ways in which the Communist party was able to appeal to certain ethnic groups, such as through cultural outreach, as well as its complicated and often arguably counter-productive relationship with the Comintern. It also furnishes important information on the efforts of the RCMP and Polish consulates to maintain control over the communists, as well as how generally improved material conditions among Poles, especially following the Second World War, along with the influence of the Cold War, accounted for a rapid decline in support. The thesis is primarily based on sources generated by the Polish Ministry of Foreign Affairs or, more precisely, by the Polish consulates in Winnipeg, Montreal and Ottawa. One the Canadian side, the thesis took advantage of RCMP records, Canadian security bulletins, immigration records and Polish-language newspapers printed in Canada. By utilizing these sources, this study not only analyses the interaction of the Polish Canadian communist movement with other segments of the Polish community in Canada, but it also moves beyond the introverted approach that has characterized most studies of ethnic organizations in Canada by placing the movement within a “Canadian” context to analyze its relations with the government, broader segments of Canadian society, and the Communist Party of Canada (CPC).

Das Biopolymer-produzierende Knallgasbakterium Ralstonia eutropha H16 gilt mit seinem außergewöhnlichen Stoffwechsel als vielversprechender Produktionsstamm für die weiße Biotechnologie. Es wächst auf einer Vielzahl organischer Substrate sowie chemolithoautotroph mit H2 und CO2 als einzige Energie- bzw. Kohlenstoffquelle. Unter anaeroben Bedingungen ist es zudem zur Denitrifikation befähigt. In dieser Arbeit wurde das Proteinprofil von R. eutropha unter chemolithoautotrophen sowie anaeroben Bedingungen mittels GeLC-MS/MS untersucht. Beide Proteomstudien offenbarten, dass die Nutzung unterschiedlicher Elektronendonoren bzw. -akzeptoren mit zahlreichen Veränderungen im Proteinbestand der Zellen einherging. Hierbei waren neben Proteinen metabolischer und Transportprozesse auch jene der Zellbewegung betroffen. Die Ergebnisse stellen im Vergleich zu vorangegangenen Studien den bisher umfassendsten Überblick zum Proteinbestand beim H2-basierten sowie anaeroben Wachstum in R. eutropha dar. Von besonderer Bedeutung war dabei das Einbinden der Analyse der Membran als Ort wichtiger Energie- und Transportprozesse. Besonderes Interesse galt einem unter H2/CO2-Bedingungen abundanten Zweikomponentensystem. Sequenzvergleiche zeigten Ähnlichkeit zum Regulationssystem der Katabolitrepression des Biphenylabbaus in Acidovorax sp. KKS102. Die Deletion des Response-Regulator-Gens führte zu vielfältigen Wachstumseffekten auf Substraten wie Fructose, Glycerin sowie auf H2/CO2. Der pleiotrope Phänotyp sowie die Ergebnisse von Genexpressionsstudien und der Suche nach Regulator-Bindestellen lassen eine globale Rolle des Systems im Energie- und/oder Kohlenstoffmetabolismus von R. eutropha H16 annehmen. Histidin-Kinase und Response Regulator wurden in GloS bzw. GloR umbenannt. Die vorliegende Arbeit zeigt eindrucksvoll das Potential der Proteomik als Teil der funktionellen Genomik für den Anstoß neuer Forschungsansätze zur Evaluierung des biotechnologischen Potentials von Mikroorganismen. / Due to its remarkable metabolism the bioplastic-producing “Knallgas” bacterium Ralstonia eutropha H16 is ranked as a promising production strain for white biotechnology. It grows on a wide range of organic substrates as well as lithoautotrophically on H2 and CO2 as sole energy and carbon source, respectively. Under anaerobic conditions it thrives by denitrification. This thesis focused on characterizing the protein profiles of lithoautotrophically and anaerobically grown R. eutropha cells. Proteome analyses revealed an extensive protein repertoire adapting the organism to alternative electron donors and acceptors, respectively. Changes concerned proteins involved in metabolic and transport processes as well as in cell movement. Compared to previous studies the results reported here offer the most comprehensive proteomic survey regarding the H2-based as well as anaerobic lifestyle of R. eutropha so far. In this context analyzing the cell membrane as a place for a number of energy, transport and signal transduction processes was of particular importance. Special interest aroused the identification of a two-component system upregulated on H2/CO2. Sequence analysis offered high similarity to the regulatory system for catabolite control of biphenyl degradation in Acidovorax sp. KKS102. Deletion of the response regulator gene led to versatile growth effects on substrates such as fructose and glycerol as well as H2/CO2. This pleiotrophic phenotype as well as the results of gene expression studies and the search for regulator binding sites suggests that the two-component system is a global player in energy and/or carbon metabolism in R. eutropha and possibly other bacteria. Thus, histidine kinase and response regulator have been renamed GloS/R. Since their characterization was initiated by proteomic data this study impressively elucidates the power of functional genomics in terms of revealing new research approaches to evaluate the biotechnological use of microbes.