In the fall of 2011, a study was initiated at the Western Kentucky University Farm in Bowling Green, Kentucky on a Crider silt loam (Typic Paleudalf). The objective of this study was to determine cultivar, mowing height, and herbicide effects on bermudagrass, Cynodon dactylon [L.] Pers., suppression in tall fescue, Schedonorus arundinaceus [Schreb.] Dumort., nom. cons. The experimental design was a split plot design with whole plots consisting of varying mowing heights and split plots consisting of different herbicide treatments with three replications. Two separate experiments were conducted. The tall fescue variety ‘KY 31’ was utilized for one study and the turf type cultivar ‘Bullseye’ was utilized in the other. In the fall of 2011, glyphosate (Roundup Pro) was applied to selected plots at the rate of 0.36 kg ae/ha. ‘KY 31’ and ‘Bullseye’ tall fescues were sown into an existing mixed stand of common and hybrid bermudagrass at 342 kg pls/ha. Mowing heights were maintained at 7.62 cm, 10.16 cm, and 12.70 cm. In the spring of 2012, treatments containing fenoxaprop (Acclaim Extra), mesotrione (Tenacity), and fluazifop (Fusilade II) were applied to selected plots at the rates of 0.07 kg ai/ha, 0.48 kg ai/ha, and 0.24 kg ai/ha respectively. A non-ionic surfactant at 0.25% (v/v) was utilized in treatments containing mesotrione and fluazifop. Three weeks later these treatments were applied a second time. Data were collected visually on turf quality, tall fescue cover, broadleaf weed cover, and bermudagrass cover. The study was repeated in 2012 – 2013. Fluazifop and glyphosate + fluazifop resulted in significantly lower turf quality than all other treatments up until ten weeks after initial treatment. Treatments containing fluazifop significantly reduced tall fescue cover but also significantly suppressed bermudagrass cover the greatest. However, these treatments also resulted in the greatest amount of broadleaf weed cover. High mowing heights may play a role in achieving high turf quality ratings and better broadleaf weed and bermudagrass suppression in forage type tall fescue than in turf type tall fescue.

The stability of railway embankment is essential for safe transport. In order to ensure safe transport, water should be allowed to freely drain. Furthermore, as the engine driver has to be able to see signs, and people working on the embankment are supposed to easily see flaws on the rails to ensure safety for the passing trains, plants should not be allowed to grow on the embankment. In Sweden there are 12 000 kilometers of railway tracks and 25–30 % of them are treated for weed control every year. The Swedish Transport Administration is currently using the pesticide “Roundup Bio” to remove weeds on the embankment. To prevent the dispersal of chemicals to sensitive ecosystems, chemical transport has to be investigated carefully. In this master thesis a transport model for the transport of pesticides has been simulated using the software “GoldSim”. The model have been developed, optimized and controlled by a sensitivity analysis. In addition to this an analysis of worst case scenarios has been tested. The pesticides “Arsenal 250” with the active substance imazapyr and “Roundup Bio” with the active substance glyphosate have been used in the calibrating of the model. The transport model for imazapyr shows a good estimation of the mass of herbicides in the embankment compared to measurements. There is also a good estimation in the groundwater even if the concentration at the beginning of the simulation period is underestimated. The simulation with glyphosate, on the on the other hand, showed very small quantities of both glyphosate and its degradation product AMPA (aminometylphosphateacid) in both the embankment and in the groundwater. The sensitivity test showed that the parameter half-life and Kd-value (adsorption capacity) were the most sensitive parameters in the model. When it comes to the concentration in the groundwater the distance to the groundwater level was the most sensitive parameter, as well as the Kd-value in the embankment, the half-life and the precipitation. As expected, the worst-case analysis showed that a small distance to the groundwater level, a low Kd-value and a short half-life produced even larger concentrations of herbicides in the groundwater. In conclusion it should be mentioned that the stimulation model generally works well in regards to with imazapyr. For stimulation of glyphosate and its metabolite AMPA more work with the model is required for the Swedish Transport Administration to apply it in the future.

Studies were conducted in 2004, 2005, and 2006 in south Texas to evaluate fall, winter, and spring weed control for commercial rice production, study tillage intensity and herbicide rate interactions for rice production, and determine the tolerance of red rice ecotypes from Texas rice fields using selected herbicides at varying rates. A single application of any herbicide or combination of herbicides was not adequate for weed control throughout the fall, winter, and spring. Fall applications of clomazone plus flumioxazin provided consistent weed control. Addition of flumioxazin to glyphosate provided excellent winter annual grass control with winter application. A residual herbicide, coupled with the proper contact herbicide is the key to extending control. In 2006, all tillage by herbicide treatments in all studies provided ≥ 90% control of all weed species. The conventional tillage treatment with low herbicide input provided the highest rice grain yield in 2005 and 2006, though they were not significantly different from the spring stale seedbed program with medium or high herbicide input in 2006. In 2006, fall stale seedbed treatments were among the lowest in yield. A stale seedbed program may be useful, but with substantial weed pressure, increasing the intensity of herbicide applications is necessary to overcome the absence of tillage. All rice ecotypes were adequately controlled by glyphosate and only one ecotype was found to be tolerant to 2x rates of both imazethapyr and imazamox. All ecotypes were adequately controlled by 2x rates of more than two of the four herbicides which included imazethapy, imazamox, glufosinate, and glyphosate. Ecotypes from the 3.2 group, genetically similar to the ecotype TX4, appear to be the most likely to exhibit tolerance to a given herbicide. Tolerance to glufosinate was found in 70% of the group 3.2 ecotypes. Sixty percent of ecotypes from group 3.1, genetically similar to Oryza rufipogon were not adequately controlled by glufosinate.

Sharppod morningglory is a perennial vine commonly found infesting croplands in Texas and the southeastern United States. Previous research regarding morningglory competition and control primarily focused on annual Ipomoea. Interference, control, and herbicide translocation of sharppod morningglory could differ from that of other morningglories because of differences in growth and resource allocation. Therefore, field and laboratory experiments were conducted from 2001 to 2004 to: 1) determine the effects of seed-propagated and root-sprouted sharppod morningglory on cotton economic value, yield, harvest efficiency, and fiber quality; 2) evaluate sharppod morningglory control with cotton herbicides, and determine the effect of diuron rates on glyphosate absorption and translocation; and 3) assess the impact of cotton herbicide program and cotton-corn rotation on weed species composition over three years. A relatively large proportion of sharppod morningglory biomass was accumulated belowground during the first 8 wk of growth in the greenhouse. Consequently, up to 6 plants 10-m row-1 did not significantly reduce cotton lint yield. Sharppod morningglory density impacted color grade more than any other classification parameter. Through combined effects on yield and quality, cotton lint value was reduced by approximately 85% in the presence of 8 sharppod morningglory 10 m-1. Glyphosate alone did not completely control sharppod morningglory. The use of glufosinate, bromoxynil, or a combination of glyphosate plus diuron provided acceptable control. Sharppod morningglory absorbed up to 75% of glyphosate when applied alone, but most glyphosate was retained in treated leaves and did not translocate well. Diuron decreased absorption, increased leaf retention, and inhibited glyphosate translocation to roots. Rotation to corn and the use of preemergence herbicides in cotton improved control of grass and broadleaf weeds during the year of treatment. In the season following the 3-yr rotation, there were no lasting effects of crop rotation on density or control of grasses and broadleaves. However, hand-hoed and herbicide treated plots resulted in weed densities 2- to 3-fold lower than the untreated. Preemergence herbicides and/or crop rotation can reduce weed density and improve weed control, but these strategies must be employed long-term to reduce density of problematic weeds through depletion of the soil seedbank.

Thesis (Ph. D.)--Ohio State University, 2008. / Title from first page of PDF file. Includes bibliographical references (p. 96-107).

The indeterminate nature of lentil (Lens culinaris Medik), in conjunction with adverse field conditions, can lead to varying degrees of maturity among plants at harvest. This variable maturity may have a negative influence on lentil production and can delay harvest. Desiccants are currently used to improve lentil crop dry-down. However, applying desiccants too early may result in reduced crop yield and quality, and also leave unacceptable herbicide residues in lentil seeds. In addition, only four herbicides (glyphosate, diquat, saflufenacil, and glufosinate) are registered as desiccants for lentil desiccation in Canada, which limits options for growers. Therefore, the objectives of this thesis were i) to determine the importance of desiccant application timing in affecting crop yield and quality, as well as herbicide residues and ii) to determine whether additional desiccants applied alone or tank-mixed with glyphosate provide better crop desiccation. Field trials were conducted at Saskatoon and Scott, Saskatchewan, from 2012 to 2014. In the application timing trial, glyphosate or saflufenacil alone, or glyphosate+saflufenacil generally decreased seed yield, thousand seed weight, and crop dry-down, and increased herbicide residue levels at earlier application timings. For example, when applied at 60% seed moisture, saflufenacil reduced yield and thousand seed weight by 22% and 10%, respectively, and resulted in glyphosate and saflufenacil residues greater than 2.0 and 0.03 ppm, respectively. Although there were no reductions in yield and thousand seed weight when desiccants were applied at 50% or 40% seed moisture, glyphosate residue exceeded 2.0 ppm. Application of desiccants at 20 or 30% seed moisture content had no effect on yield, thousand seed weight, or herbicide residues. These results indicate that desiccant application timing is critical, and should not be made before 30% seed moisture. In a second study, glufosinate and diquat tank mixed with glyphosate were the most consistent desiccants and provided optimal crop dry-down without reducing yield and thousand seed weight, and effectively reduced glyphosate residue. The other herbicides tested (pyraflufen-ethyl and flumioxazin) were found to be poor options for growers as they had sub-optimal crop desiccation and did not affect glyphosate residue.

Giant ragweed is an extremely competitive weed and poor control in soybean could lead to significant yield losses for Ontario producers. In 2008, a giant ragweed biotype near Windsor, ON was not controlled with glyphosate and further testing confirmed it as the first glyphosate-resistant (GR) weed in Canada. Giant ragweed seed was collected from 102 locations in Essex (70), Kent (21), Lambton (10) and Waterloo (1) counties to document the occurrence and distribution of GR giant ragweed in Ontario. Giant ragweed seedlings were sprayed with glyphosate at 1800 g a.e. ha-1, and evaluated 1, 7, 14 and 28 days after application (DAA). Results from the survey concluded that there are 47 additional locations in southwestern Ontario with GR giant ragweed. The majority of the sites were found in Essex county, but there was one location in both Chatham-Kent and Lambton counties. Field trials were established at six sites with GR giant ragweed during the 2010 and 2011 growing seasons. The objectives were to determine the level of giant ragweed control with increasing doses of glyphosate, and glyphosate tank mixes applied either preplant or postemergence. Control of giant ragweed increased with higher doses of glyphosate, but only at doses that are not economical for producers. The most effective glyphosate tank mixes were 2, 4-D ester, saflufenacil, linuron, and cloransulam-methyl providing up to 98, 94, 99 and 97% control 4 weeks after application (WAA), respectively. Glyphosate plus dicamba in dicamba-tolerant soybean provided up to 100% giant ragweed control, 4 WAA at the three confined field trial locations. / Monsanto Canada Inc.; Canadian Agricultural Adaptation Program; Grain Farmers of Ontario

Cyperus rotundus L. CYPRO (purple nutsedge) and Cyperus esculentus L. CYPES (yellow nutsedge) are problematic weeds on every continent. At present there is no comprehensive means of controling these weeds.. The primary means of control is herbicides, although the weeds are becoming more resistant. Bioherbicide control of purple and yellow nutsedge is an important avenue of research, with much of the focus being to increase the virulence of current fungal pathogens of C. rotundus and C. esculentus. The primary aim of this study was to increase the virulence of a fungal pathogen of C. rotundus and C. esculentus, with the objective of creating a viable bioherbicide. A possible means of increasing the virulence of a pathogen would be to increase the amount of amino acid produced by the fungus. This was proposed as a means of increasing the virulence of Dactylaria higginsii (Luttrell) M. B. Ellis. Overproduction of amino acids such as valine and leucine result in the feedback-inhibition of acetolactate synthase (ALS), an enzyme which is a target for many herbicides currently on the market. By applying various amino acids to tubers of purple nutsedge and comparing the results with a reputable herbicide, glyphosate, it was possible to determine the success of the amino acid applications. Only glutamine treatment at 600 mg.r1 resulted in significantly less (P<O.OOI) germination compared with the water control, while the glyphosate application resulted in no germination. Four treatments were significantly different (P<O.OOI) from the water control in terms of shoot length, but no pattern or conclusion could be drawn from the results. Injecting amino acids and glyphosate into the leaves of the plants gave similar results to those obtained with the tubers, with no visible damage on those plants injected with the amino acids and complete plant death of those injected with glyphosate. Amino acids had little effect on the growth of the C. rotundus plant or tuber. It was later determined by a colleague (Mchunu1 , unpublished) working on the same project, that D. higginsii does not infect the local ecotypes of C. rotundus in Pietermaritzburg, South Africa. A second fungus, Cercospora caricis Oud., was isolated from C. rotundus growing in the region, and confirmed as a Cercospora species by conidial identification. Like many Cercospora species, C. caricis produces a phytotoxin, cercosporin. An increase in production of cercosporin would theoretically lead to an increase in virulence of C. caricis. Mutation of hyphae by i J Makhosi Mchunu: Address: National department ofAgriculture; Private Bag 3917; Port Elizabeth; 6056 Email: Makhosimc@NDA.agric.za ultraviolet-C light was perfected on C. penzigii Sacc., where 5 min exposure to DV-C light resulted in approximately 99% cell death. Surviving colonies were analysed by spectrophoresis, and the surviving mutant gave an absorbance value of approximately 5% more than the median. Samples were analysed by high-performance liquid chromatography (HPLC) to determine the presence of cercosporin. No definitive result was obtained. Exposure of C. caricis to DV-C for 5 min. resulted in approximately 65% hyphal cell death, with 20 min. resulting in approximately 95% death. A spontaneous mutant was observed in a colony that had been exposed to DV-C. This mutant showed sectored growth with red and grey growth patterns. The red section of the mutant was subcultured and analysed by spectrophoresis and HPLC. The red C. caricis gave an absorbance reading of approximately 140 on HPLC compared with about 22 from the grey colony. HPLC analysis of the wild-type C. caricis did not produce a peak corresponding to that of the cercosporin standard, although no conclusion could be obtained on the presence or absence ofthe toxin. The virulence of the mutant C. caricis could not be determined as inoculation experiments were unsuccessful, and had to be discontinued due to time constraints. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.

Reduced plant population in glyphosate-resistant soybean [Glycine max (L) Merr.] may influence the critical time of herbicide application. Field studies were conducted in 2007 and 2008 at two locations in Kentucky to determine the effect of four weed control programs on soybean seed yield, seed quality, crop canopy, and weed pressure when planted at three densities - 185,000, 309,000, and 432,000 plants ha-1. Plots were treated with glyphosate at either 3 weeks after planting (WAP), 5 WAP, 7 WAP, 3 & 7 WAP, representing common weed control protocols within the state. No differences in seed yield were discovered between plant densities in two of four siteyears. 254,500 plants ha-1 was sufficient for maximum yields in all site-years and was also sufficient to achieve maximum canopy amongst those populations tested in the study. Sequential applications at 3 and 7 WAP provided the highest seed yield, while the 5 WAP and 7 WAP application timings were generally the single applications that allowed for the highest yield and canopy closure, along with the highest visual estimate of weeds controlled. Plant density did not influence the critical period for weed control.

Tests were conducted for 71 pesticides in weekly bulk (wet + dry) deposition samples collected from May 25 to September 21 over two years at two sites in the City of Winnipeg, Canada. Twenty-one pesticides and their metabolites were detected in this study and 99% of samples collected contained mixtures of two or more pesticides. Malathion and glyphosate were the largest contributors to bulk deposition in 2010 and 2011, respectively. A second study examined the mineralization of 2,4-D and 17β-estradiol using a novel in-field soil microcosm study and a series of laboratory experiments under different temperature incubations. Results indicated that temperature fluctuations do not greatly affect the amount or rate of mineralization relative to the traditionally constant laboratory incubations of 20°C; however long-term freezing of soil reduced potential mineralization over time. This research advances scientific knowledge of agri-chemical fate and provides data for strengthening current environmental policy analysis in Canada.