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Additional articles by author bound in back. / Bibliography: leaves 136-149 / ix, 150 leaves : ill., (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This investigated focussed on selected aspects of berry development and ripening that were subject to variation. Shiraz and Chardonnay were chosen as experimental varieties because these cultivars presented a large range of variability in the field. The extent of the variation within each of the recorded berry parameters was assessed using the coefficient of variation (CV), a unitless measure of sample variability relative to the sample mean, ideally suited to comparative studies. / Thesis (Ph.D.)--University of Adelaide, Dept. of Horticulture, Viticulture and Oenology, 2002

"Department of Horticulture, Viticulture and Oenology, Faculty of Agriculture and Natural Resource Sciences, The University of Adelaide, Waite Campus, Glen Osmond S.A." Bibliography: leaves 136-149 This investigated focussed on selected aspects of berry development and ripening that were subject to variation. Shiraz and Chardonnay were chosen as experimental varieties because these cultivars presented a large range of variability in the field. The extent of the variation within each of the recorded berry parameters was assessed using the coefficient of variation (CV), a unitless measure of sample variability relative to the sample mean, ideally suited to comparative studies.

Thesis (MSc)--Stellenbosch University, 2002. / ENGLISH ABSTRACT: The cultivation of table grapes in the warmer areas of South Africa, indeed worldwide, is complicated by rest breaking problems in spring due to delayed budbreak. In order to overcome these problems rest breaking agents, mainly hydrogen cyanamide, are applied. However, instead of alleviating the problem, additional problems such as uneven budbreak and reduced production are often induced. This study was initiated to further understand the physiological processes occurring during budbreak and how the application of hydrogen cyanamide influences these processes. The following aspects were investigated in this study: a. The effect of hydrogen cyanamide on tissue cytokinin (specifically zeatin riboside) levels of Sultanina table grape vines after application at different times before natural budbreak was studied over two seasons. In 1997, hydrogen cyanamide was applied at three weeks before induced budbreak and in 1998 at six weeks before induced budbreak. One year-old canes were sampled weekly after hydrogen cyanamide application, divided into distal and proximal sections, then further divided into buds, bark and wood tissues and the zeatin riboside (ZR) levels determined. A relatively high amount of chilling coupled to late hydrogen cyanamide application in 1997 led to a large effect on ZR release, but did not lead to significant shifting of the budbreak pattern. Zeatin riboside peaks were observed in buds, internode wood and bark of treated vines compared to control vines. The peaks were higher in distal portions compared to proximal portions in all tissues. The relatively lower chilling and earlier application of hydrogen cyanamide in 1998 had a larger effect on the budbreak pattern while the bud ZR peak was shifted earlier. The distal portion bud ZR . peak was again higher than the proximal portion bud ZR peak. In 1997, as sampling was not initiated early enough, bud ZR peaks were only observed after budbreak, while in 1998 bud ZR peaks were observed before and after budbreak. The effect of these ZR increases on the development of inflorescence primordia, subsequent bunch development and ultimately production, are discussed. b. Free xylem sap was sampled at cane and spur pruned lengths from unpruned canes of Sultanina from budswell until after budbreak in 1999 and from three table grape cultivars, i.e Sultanina, Alphonse Lavalleé and Sunred Seedless, in 2001 and ZR levels determined. The ZR levels in the buds of these three table grape cultivars, pruned to different cane lengths were also determined. One year old canes of these cultivars, were each pruned to long canes (14 buds) and short spurs (2 buds). The ZR content in buds of these canes at distal and proximal positions were determined weekly from budswell until after budbreak in 1999. Xylary ZR peaks occurred before 50% budbreak. Spur xylary ZR levels of all three cultivars followed a similar pattern, although at lower ZR levels than that of the canes. This is similar to previous studies on xylary ZR levels of apple shoots. The high levels of free ZR found in xylem sap at the distal portions of canes support the hypothesis of a cumulative ZR build-up effect as cane length increases. Spur pruning resulted in earlier budbreak and a higher final budbreak than cane pruning. The proximal portions of shoots, whether spur pruned or the proximal portions of canes, showed elevated ZR levels in all cultivars. This difference in ZR levels in bud tissue of different portions of the cane would suggest a difference in ZR consumption or turnover. The results of this study have important management implications for the cultivation of vines in warmer areas in which hydrogen cyanamide is used to alleviate budbreak problems. / AFRIKAANSE OPSOMMING: Die verbouing van tafeldruiwe in die warmer gebiede van Suid-Afrika, soos straks wêreldwyd, word bemoeilik deur rusbreekprobleme in die lente weens vertraagde bot. Rusbreekmiddels, meestal waterstofsiaanamied, word gevolglik toegedien om hierdie probleme te probeer oorkom. In plaas van opheffing van die probleem, veroorsaak hierdie toedienings somtyds addisionele probleme soos ongelyke bot en verlaagde produksie. Hierdie studie is aangepak om die fisiologiese prosesse wat tydens bot plaasvind, beter te verstaan, asook hoe die toediening van waterstofsiaanamied hierdie prosesse beïnvloed. Die volgende is in hierdie studie ondersoek: a. Die invloed van waterstofsiaanamied op lootweefsel sitokinien (naamlik zeatin ribosied, ZR) vlakke van 'n Sultanina tafeldruif wingerd is oor twee seisoene ondersoek na toediening op verskillende tye voor bot. Waterstofsiaanamied is in 1997 drie weke en in 1998 ses weke voor geïnduseerde bot toegedien. Een jaar oue lote is weekliks gemonster na waterstofsiaanamied toediening, verdeel in apikale en basale gedeeltes en verder verdeel in ogies, bas en houtweefsel en die ZR vlakke bepaal. Relatief hoër vlakke koue, gekoppel met later watersiaanamied toediening het in 1997 tot 'n groot effek op ZR vlakke gelei, maar het nie die botpatroon wesentlik verskuif nie. Zeatin ribosied pieke is waargeneem in ogies, internode hout en bas van behandelde wingerd in vergelyking met kontrole wingerd. Die pieke was hoër in apikale gedeeltes in vergelyking met basale gedeeltes in alle weefsels. Die relatief laer koue en vroeër toediening van waterstofsiaanamied in 1998 het 'n groter effek op die botpatroon gehad, terwyl die ogie ZR piek vroeër geskuif is. Die apikale gedeelte se ogie ZR piek was weereens hoër as die basale gedeelte se ogie ZR piek. Monstering was nie vroeg genoeg begin in 1997 nie, aangesien ogie ZR pieke slegs na bot waargeneem is, terwylogie ZR pieke in 1998 voor en na bot waargeneem is. Die effek van hierdie verhoging in ZR vlakke op die ontwikkeling van blom primordia, daaropvolgende tros ontwikkeling en uiteindelik produksie, is bespreek. b. Vry xileemsap is gemonster van langdraer en kortdraer gedeeltes van ongesnoeide lote van Sultanina vanaf ogieswel tot na bot in 1999 en vanaf drie tafeldruif kultivars, naamlik Sultanina, Alphonse lavalleé en Sunred Seedless, in 2001 en die ZR vlakke bepaal. Die ZR vlakke van ogies van hierdie drie kultivars, gesnoei tot verskillende lootlengtes is ook bepaal. Eenjarige lote van hierdie kultivars is gesnoei tot langdraers (14 ogies) en kortdraers (2 ogies). Die ZR vlakke in ogies geleë op apikale en basale gedeeltes van hierdie lote is weekliks in 1999 bepaal vanaf ogieswel tot na bot Xileemsap ZR pieke is waargeneem voor 50% bot. Kortdraer xileemsap ZR vlakke het 'n soortgelyke patroon as die langdraers gevolg vir al drie kultivars, alhoewel teen laer ZR vlakke as die langdraers. Hierdie waarneming is soortgelyk aan vorige studies op xileemsap ZR vlakke van appel lote. Die hoë ZR vlakke gevind in die xileemsap van die apikale gedeeltes van lote ondersteun die hipotese van 'n kumulatiewe opbou van ZR soos die lengte van die loot toeneem. Die snoei van lote as kortdraers het gelei tot vroeër bot en 'n hoër finale bot persentasie as die snoei van langdraers. Die basale gedeeltes van lote het verhoogde ZR vlakke in al drie kultivars getoon, onafhanklik van die feit dat dit vanaf die kortdraer of die basale gedeelte van 'n langdraer was. Hierdie verskil in ZR vlakke in ogie weefsel van verskillende gedeeltes van die loot impliseer 'n verskil in ZR verbruik of omset. The resultate van hierdie studie het belangrike bestuursimplikasies vir die verbouing van wingerd in warmer gebiede, waar waterstofsiaanamied gebruik word om botprobleme te oorkom.

Thesis (PhD) -- Stellenbosch University, 1991. / ENGLISH ABSTRACT: The effect of partial defoliation as canopy management practice on metabolism and grape composition of the grapevine, Vitis vinifera L cv. Cabernet Sauvignon, was investigated. The establishment of physiologically sound principles on how to overcome deleterious effects associated with vigorous and dense-canopy vines is emphasized. Experimental vines were defoliated 33 % and 66 % evenly over the whole canopy from different developmental stages in a field study. Effects on canopy microclimate, photosynthesis, photosynthate translocation, vegetative growth, reproductive growth, root development and distribution, as well as grape and wine quality, were determined. A method for the simultaneous extraction of sugars and organic acids from freeze-dried berries at different developmental stages is described. Partial defoliation of vines improved canopy microclimate and photosynthetic efficiency of remaining leaves. Normal translocation and distribution patterns of photosynthates were apparently unaffected by partial defoliation. Translocation to and accumulation of photosynthetic products in the leaves and bunches of partially defoliated vines were, however, improved. Remaining leaves of partially defoliated vines were in comparison photosynthetically more active. Apart from a less favourable canopy microclimate, it seemed that the sink capacity of non-defoliated vines did not comply to the source capacity, inducing a reduced rate of photosynthesis. Normal sigmoidal growth patterns of vines were not affected by partial defoliation as applied in this study. This is important for the longevity, healthiness and productivity of vines. Vegetative growth was differentially affected by partial defoliation. No compensatory leaf growth occurred in reaction to partial defoliation from different developmental stages. Main shoot length, however, decreased slightly. Lateral shoot length and number of laterals increased, whereas cane mass decreased when vines were partially defoliated, particularly the earlier and more severe the defoliation. Reproductive growth in terms of yield was deleteriously affected by 33 % defoliation prior to pea size and 66 % defoliation prior to veraison. Budding percentage was, however, improved by 33 % and 66 % defoliation, whereas bud fertility was only improved by 33 % defoliation. Partial defoliation changed the canopy microclimate to conditions favourable for pest and disease control and higher grape quality. Subterranean growth was favourably affected by partial defoliation, particularly when applied from pea size stage. These changes included higher root densities, development of higher numbers of fine and medium diameter roots and occurrence of higher total root numbers in all soil layers. Generally, defoliations from pea size and veraison were more efficient regarding root development than defoliations from just after bud break and from berry set. Partially defoliated vines reacted by forming new roots, creating a more efficient nutrient absorption capacity and utilization of soil and available water and that, together with higher photosynthetic activities of leaves, provided an efficient mechanism for continued high performance. Grape quality was not affected markedly by partial defoliation. Total soluble solids in berries of defoliated vines were comparable to and even significantly higher than those of non-defoliated vines in some cases, in spite of much lower leaf areas. Generally, total titratable acidity of musts was also slightly higher for partially defoliated vines. Glucose and fructose concentrations in berries were unaffected by partial defoliation, while tartaric acid concentrations were slightly increased and malic acid concentrations slightly decreased. Partial defoliation generally increased the anthocyanin concentration of berry skins. These changes in grape composition suggest higher grape quality and seemed to result from improved light conditions in the canopy interior. Berry volume decreased with partial defoliation, which lowered the pulp:skin ratio. These berries are more desirable for quality wines. Regardless of severity or the developmental stage defoliation was commenced, wine cultivar character and overall wine quality were significantly improved. Partial defoliation changed the general metabolism of vines, mainly in terms of more favourable source:sink ratios, resulting in more efficient photosynthesis, subterranean performance and canopy microclimate. In general, the results suggest that an even removal of 33 % of leaves opposite and below bunches during the period from flowering or berry set to pea size stage may be applied. lt is further suggested that existing vigorous and dense-canopy vines be 33 % defoliated evenly on the lower half of the shoot (canopy) from pea size or veraison. This hypothesis proved effective in improving canopy microclimate, photosynthetic activity and yield, while vegetative growth was inhibited. Grape and wine quality were higher. On the whole, partial defoliation as applied in this study, is recommended as canopy management practice in order to facilitate the abolishment of deleterious effects of excessive vegetative growth and canopy density on balanced metabolic activity, fruit and wine quantity and quality, as well as longevity and healthiness of grapevines. / AFRIKAANSE OPSOMMING: Die invloed van blaarverwydering as loofbestuurspraktyk op die metabolisme en druifsamestelling van die wingerdstok, Vitis vinifera L cv. Cabernet Sauvignon, is ondersoek. Die vestiging van fisiologies-betroubare beginsels oor hoe nadelige effekte geassosieerd met geil en lower-verdigte wingerde uitgeskakel kan word, word beklemtoon. Proefstokke is in 'n veldondersoek 33 % en 66 % eweredig oor die hele lower vanaf verskillende ontwikkelingstadiums ontblaar. Effekte op lowermikroklimaat, fotosintese, translokasie van fotosintetiese produkte, vegetatiewe groei, reproduktiewe groei, wortelontwikkeling en verspreiding asook druif- en wynkwaliteit is bepaal. 'n Metode vir die gelyktydige ekstraksie van suikers en organiese sure uit gevriesdroogde korrels op verskillende groeistadiums is ontwikkel. Blaarverwydering het lowermikroklimaat verbeter en fotosintetiese doeltreffendheid van oorblywende blare op die stok verhoog. Normale translokasie en verspreidingspatrone van produkte van fotosintese is skynbaar nie deur blaarverwydering befiwloed nie. Translokasie na, en akkumulering van fotosintetiese produkte in die blare en druiwe van gedeeltelik ontblaarde stokke, is egter verbeter. Oorblywende blare van gedeeltelik ontblaarde stokke was fotosinteties meer aktief. Afgesien van 'n ongunstiger lowermikroklimaat, het die sinkkapasiteit van nie-ontblaarde stokke skynbaar ook nie teen die bronkapasiteit opgeweeg nie en is 'n verlaagde tempo van fotosintese verkry. Normale sigmofdale groeipatrone van die stokke is nie deur blaarverwydering befrivloed nie. Dit is belangrik vir langlewendheid, gesondheid en produktiwiteit van stokke. Vegetatiewe groei is differensieel deur blaarverwydering befrivloed. Geen kompenserende blaargroei het in reaksie op blaarverwydering vanaf verskillende ontwikkelingstadiums voorgekom nie. Hooflootlengte was egter effens korter. Sylootlengte en aantal sylote het toegeneem, terwyl lootmassa afgeneem het met blaarverwydering, veral hoe vroeer en strawwer dit toegepas is. Reproduktiewe groei in terme van opbrengs is nadelig befrivloed deur 33 % ontblaring voor ertjiekorrelstadium en 66 % ontblaring voor die deurslaanstadium. Botpersentasie is egter deur blaarverwydering verbeter, terwyl oogvrugbaarheid slegs deur 33 % ontblaring verbeter is. Blaarverwydering het 'n gunstige lowermikroklimaat vir die beheer van plae en siektes en hoer druifkwaliteit geskep. Ondergrondse groei is gunstig deur blaarverwydering befrivloed, veral wanneer toegepas vanaf ertjiekorrelstadium. Hierdie veranderinge het ingesluit hoer worteldigtheid, ontwikkeling van groter hoeveelhede wortels met 'n fyn en medium deursnit en die voorkoms van 'n groter aantal totale wortels in alle grondlae. Blaarverwydering vanaf ertjiekorrel- en deurslaanstadia was in die algemeen meer doeltreffend ten opsigte van wortelontwikkeling as blaarverwydering vanaf net na bot en vanaf korrelset. Gedeeltelik ontblaarde stokke het gereageer deur nuwe wortels te vorm, waardeur 'n meer doeltreffende vermoe tot voedingstofopname en benutting van grand an beskikbare water verkry is. Tesame met hoer fotosintetiese aktiwiteite van die blare is 'n doeltreffende meganisme vir aanhoudende hoe prestasie verkry. Druifkwaliteit is nie aanmerklik deur blaarverwydering beirwloed nie. Totale oplosbare stowwe in druiwe van gedeeltelik ontblaarde stokke was vergelykbaar en selfs betekenisvol hoer as die van nie-ontblaarde stokke in sekere gevalle, ten spyte van die baie laer blaaroppervlakte. In die algemeen was die totale titreerbare suur in die mos van gedeeltelik ontblaarde stokke oak effens hoer. Glukose- en fruktosekonsentrasies in die druiwe is nie deur blaarverwydering beinvloed nie. Die konsentrasie wynsteensuur is egter effens verhoog en die appelsuurkonsentrasie effens verlaag. Antosianienkonsentrasie van korreldoppe is in die algemeen deur blaarverwydering verhoog. Hierdie veranderinge in druifsamestelling dui op hoer druifkwaliteit en is skynbaar die resultaat van verbeterde ligtoestande in die binnekant van die lower. Korrelvolume het afgeneem met blaarverwydering. Dit het die pulp:dop verhouding verlaag. Sulke korrels is meer gewens vir bereiding van kwaliteitswyne. Wyn cultivarkarakter en totale wynkwaliteit is betekenisvol deur blaarverwydering verhoog, onafhanklik van die strafheid of ontwikkelingstadium waarvandaan blare verwyder is. Blaarverwydering het die algemene metabolisme van die wingerdstok verander, hoofsaaklik ten opsigte van meer gunstige bron:sink-verhoudings en derhalwe meer doeltreffende fotosintese, ondergrondse groei en lowermikroklimaat. Die resultate dui in die algemeen daarop dat 'n eweredige blaarverwydering van 33 % regoor en onderkant die trosse tydens blomvorming of korrelset tot ertjiekorrelstadium toegepas kan word. Die resultate dui verder daarop dat 33 %van bestaande geil en lower-verdigte stokke se blare eweredig verwyder kan word op die onderste helfte van die loot (lower) vanaf ertjiekorrel- of deurslaanstadium. Hierdie hipotese was suksesvol in die verbetering van lowermikroklimaat, fotosintetiese aktiwiteit en oesmassa. Vegetatiewe groei is gestrem. Druif- en wynkwaliteit was hoer. In die geheel kan blaarverwydering soos toegepas in hierdie ondersoek aanbeveel word as loofbestuurspraktyk ten einde die uitskakeling van nadelige effekte van oormatige vegetatiewe groei en lowerdigtheid op gebalanseerde metaboliese aktiwiteit, druif- en wynkwantiteit en kwaliteit, asook langlewendheid en gesondheid van wingerdstokke te bevorder.

The effect of hydrogen cyanamide (H₂CN₂) on budbreak and fruit maturity of 2-year old 'Flame Seedless' table grapes was investigated in a low desert climate in central Arizona. Dormant sprays of H₂CN₂ applied at 2.5 and 5.0% (v/v) concentrations hastened budbreak by at least 16 days with subsequent advancement of fruit maturity by 5 to 10 days. A 5.0% (v/v) dormant application of H₂CN₂ to whole vine, buds only or pruning cuts only advanced budbreak by at least 16 days and advanced fruit maturity by 1 to 5 days, regardless of application site. A more uniform budbreak was observed on H₂CN₂ treated vines, regardless of concentration or site of application when compared to control vines.

Thesis (MScAgric)--Stellenbosch University, 2003. / ENGLISH ABSTRACT: In the past it was very difficult to visualise the extent and distribution of variability in growth vigour within vineyards. The advancement of remote sensing technology has changed this however, establishing new methods to assess and manage variability. Even though the causes and effects of within-vineyard variability in growth vigour are complex, new technologies offer better ways of monitoring, researching and managing these factors. In addition to the possible benefits of aerial or satellite remote sensing, new methods of mapping soil spatial variability as well as advances in georeferencing technologies supply precision tools to both researchers and producers. The scientific advancement of the technology, however, is currently the most important aspect needed. This is crucial to answer and explore fundamental questions regarding the use of the technology and the interpretation of results within the framework of the plant's observed reaction. Only then can the possible applications in vineyard management be optimised to address the management problems of extensive within-vineyard variation in growth vigour. The choice will always be to use the technology to manage the observed variability in order to limit the negative impacts of a heterogeneous harvest, or to identify the variability and its causes for the implementation of management practices aimed at a more homogenous vineyard and harvest. Whatever the case, extensive research is needed to provide tight correlations between information gathered with new technologies to assess variability and plant status, such as multi- and hyperspectral analysis, and ground-truthed results in the vineyard. Only then will it become evident which methods and analyses would be useful in the drive towards in-depth analysis and management of vineyards within the concept of precision viticulture and its derived advantages. With this in mind, the aim of this study was to establish an experimental model to use remote sensing technologies to identify and classify within vineyard variability with a subsequent analysis of the causes of variability and the effects on the plants. The targeted experimental model was a vineyard with highly heterogeneous above-ground growth. An aerial photograph of the vineyard was studied and manipulated to yield image pixel values used to quantify the degree of variability for different plots, which were chosen according to different plot layouts. Soil conditions were assessed on both a global and plot level, with extremely high pH and low resistance values in the soil in combination with erratic soil preparation practices found to be the main cause of variability. Soil physicochemical condition was also assessed during a soil profile pit study. Significant differences were found between several soil-related parameters measured for the higher and lower vigour levels and a strong correlation was also found between the resistance of a saturated soil paste and the image pixel values. Vegetative measurements also yielded highly significant differences between the vigour levels and confirmed the suitability of the vineyard to study within-vineyard variability. Some of these measurements were also strongly correlated with soil conditions as well as image pixel values. Trunk circumference proved to be an excellent measure for the level of variability, being linked strongly to canopy characteristics, soil conditions as well as the image pixel values. Leaf water potential measurements also yielded significant differences between the vigour levels. Harvest data and wine analyses showed the effect that vigour differences can have on grape composition and wine quality, even though the differences found here were much less than expected. Even though no clear preference was shown between the wines made from the different vigour levels, the lower vigour wine was considered fruitier. The overall quality of both experimental wines was however very high, considering that experimental winemaking techniques has been used. Hyperspectral measurements also confirmed differences between the vigour levels through a narrow-band NOVI (normalised difference vegetation index). It was also possible to show differences in certain biochemical compounds between the vigour levels on both a leaf and canopy level. Wavelength regions corresponding to carotenoid, chlorophyll a and chlorophyll b showed different spectral reactions in the leaves of more stressed (lower vigour) canopies, indicating possibilities for further studies. This study and its results is the first of its kind in the South African wine industry and paves the way for more focussed and in-depth analyses of the use of specifically multiand hyperspectral data to accurately assess within-vineyard vigour variability and the management thereof to yield optimum quality grapes for a specific wine target. Moreover, the approach adopted in this study is also echoed in other international research programs in prominent wine countries. The availability of scientific research regarding the optimal use and limitations of these technologies has the potential to revolutionise production management practices in the next few years in the viticultural industry. / AFRIKAANSE OPSOMMING: In die verlede was dit baie moeilik om die omvang en verspreiding van groeikragvariasie binne 'n wingerd te visualiseer. Die vordering gemaak op die gebied van afstandswaarneming-tegnologie het egter nuwe metodes beskikbaar gestel waardeur hierdie variasie in wingerde gemonitor en bestuur kan word. Selfs al is die oorsake en invloede van binne-wingerd-groeikragvariasie kompleks, verskaf nuwe tegnologieë verbeterde metodes om hierdie variasie te monitor, te bestuur en na te vors. Saam met die moontlike voordele wat lugfoto's en satelliet-afstandswaarneming teweegbring, verskaf nuwe metodes om ruimtelike variasie in grondfaktore te karteer, asook vordering in geoverwysingstegnologie, presisiehulpmiddels aan produsente én navorsers. Die wetenskaplike vordering van dié tegnologie is tans van groot belang. Die belang daarvan is om fundamentele vrae te ondersoek en te beantwoord rakende die gebruik van die tegnologie en die interpretasie van resultate binne die raamwerk van die waargeneemde reaksie in die plant. Dit sal die weg baan vir optimale toepassing van die tegnologie in wingerdbestuur om sodoende die bestuursprobleme wat deur binne-wingerdgroeikragvariasie teweeggebring word, aan te spreek. Die voorkeurkeuse is om dié tegnologie aan te wend om hierdie variasie te bestuur sodat die negatiewe impak van In heterogene oes teengewerk kan word, of om die variasie te identifiseer vir die implementering van bestuurspraktyke gemik op die skep van 'n meer homogene wingerd en oes. Dit is noodsaaklik dat uitgebreide navorsing gedoen word om noue verwantskappe vas te stel tussen inligting wat ingewin is met behulp van nuwe tegnologieë wat die variasie in plantstatus monitor, soos multi- en hiperspektrale analise, en inligting wat op grondvlak ingewin is. Hieruit sal dit duidelik wees watter metodes en analises die doeltreffendste is vir in-diepte analises en die bestuur van wingerde binne die konsep van presisie-wingerdkunde. Met inagneming van hierdie aspekte, was die doel van hierdie studie om 'n eksperimentele model daar te stel waardeur afstandswaarneming-tegnologie gebruik kan word om variasie binne wingerde te identifiseer en te klassifiseer deur analises van die oorsake van hierdie variasie en invloede op die plant. Die geteikende eksperimentele model was 'n wingerd met hoogs heterogene bogrondse groei. 'n Lugfoto van die wingerd is bestudeer en gemanipuleer om pixelwaardes te verskaf wat die graad van variasie vir verskillende eksperimentele plotte, wat aan die hand van verskillende plotuitlegte gekies is, te kwantifiseer. Grondtoestande is bestudeer op 'n globale én plotvlak, met uiters hoë pH en lae weerstande in kombinasie met verkeerde grondvoorbereidingspraktyke, wat geïdentifiseer is as die hoofoorsake vir die hoë vlakke van variasie. Grondfisiese en -chemiese toestand is ook tydens profielgatstudies bestudeer. Betekenisvolle verskille is gevind tussen verskeie grondverwante parameters gemeet vir plotte met onderskeidelik laer en hoër groeikrag, en 'n sterk verwantskap is gevind tussen grondweerstand en pixelwaardes, soos vanaf die lugfoto bepaal. Vegetatiewe metings het ook betekenisvolle verskille tussen die hoër en laer groeikragvlakke opgelewer, wat die geskiktheid van die wingerd vir die studie van binnewingerdvariasie in groeikrag bevestig het. Van hierdie metings was ook nou verwant aan grondtoestande, asook beeldpixelwaardes. Stamomtrek was 'n uitstekende maatstaf vir die vlakke van variasie, aangesien dit nou verwant was aan lowertoestande, grondtoestande, asook beeldpixelwaardes. Blaarwaterpotensiaal-metings het ook betekenisvolle verskille tussen die hoër en laer groeikragvlakke opgelewer. Oesdata en wynanalise het die uitwerking van groekragverskille op druifsamestelling en wynkwaliteit uitgewys, selfs al was die verskille wat gevind is minder as wat verwag is. Hoewel geen duidelike voorkeur tussen die wyne afkomstig van verskillende groeikragvlakke uitgewys kon word nie, was die wyn wat van die laer-groeikrag stokke gemaak was, meer vrugtig. Die algemene kwaliteit van beide wyne was egter baie hoog as in ag geneem word dat eksperimentele wynmaakprosedures gevolg is. Hiperspektrale metings het ook die verskille tussen groeikragvlakke bevestig deur 'n nou-bandwydte NDVI ("normalised difference vegetation index"). Dit was ook moontlik om verskille in sekere biochemiese komponente tussen die groeikragvlakke op 'n blaar- én lowervlak uit te wys. Golflengte-areas ooreenstemmend met karotenoïed, chlorofil a en chlorofil b het verskillende spektrale reaksies in die blare met hoër stresvlak (laer groeikrag) lowers ten toon gestel. Dit het moontlikhede vir verdere navorsing uitgewys. Hierdie studie en die resultate wat verkry is, is die eerste van sy soort in die Suid- Afrikaanse wynbedryf. Dit baan die weg vir meer gefokusde en in-diepte analise van die gebruik van spesifiek multispektrale en hiperspektrale data om binne-wingerdgroekragvariasie akkuraat te monitor en te bestuur met die oog op optimum wynkwaliteit vir 'n spesifieke produkdoelwit. Die aanslag van hierdie navorsing is ook sigbaar in ander prominente wynproduserende lande. Beskikbaarheid van wetenskaplike navorsing rakende die optimale gebruik en tekortkominge van hierdie tegnologieë het die potensiaal om produksiebestuurspraktyke in die wingerdbedryf in die komende jare te revolusionaliseer.

Thesis (MScAgric)--Stellenbosch University, 2004. / ENGLISH ABSTRACT: The effect of shoot heterogeneity on vegetative and reproductive growth parameters, vine physiology and grape composition was investigated in a ShirazlRichter 99 vineyard. Comparisons between underdeveloped (typically shorter and less ripened at véraison) and normally developed shoots in both shaded (non-manipulated) and well-exposed (manipulated) canopies were made. Compared to underdeveloped shoots, normal shoots had a larger total leaf area, due to the higher occurrence of secondary shoots as well as larger leaves on primary and secondary shoots. Since the photosynthetic activity of the leaves from normal shoots was higher than those from underdeveloped shoots, higher levels of carbohydrates were produced and stored in the former. Starch was more evenly distributed over the length of the whole shoot in normally developed shoots compared to underdeveloped shoots. Normally developed shoots were longer and thicker in diameter than underdeveloped shoots. The larger clusters of the normally developed shoots are evidence of their more favourable total leaf area per gram berry mass. Berries from the normally developed shoots were smaller at five weeks after véraison than those from underdeveloped shoots, displaying a higher skin to pulp ratio and therefore higher anthocyanin and total phenolic extraction potential for winemaking. The smaller clusters and fewer berries per cluster found for the underdeveloped shoots indicate an imbalance between vegetative and reproductive growth initiated during the vegetative growth phase and continued during the ripening period. The peculiar absence of statistically significant differences in grape composition between normally and underdeveloped shoots indicates that assimilates needed for berry ripening of the latter originated in organs other than the leaves [e.g. from adjacent normal shoots and the rest of the permanent structure of the vine (cordon, trunk, roots)]. The larger differences in berry size that occurred between shoot types in the shaded compared to the well-exposed canopies may be evidence for this. The photosynthetic activity of shoots was lower in shaded than in exposed canopies. The total carbohydrate production of the normal shoots in shaded canopies seemed insufficient to supply in the ripening needs of the shoot itself, their own clusters as well as the ripening of stem tissue and clusters of the underdeveloped shoots in the canopy. This is illustrated by the lower levels of starch that accumulated in the normal shoots from shaded compared to that of exposed canopies. Vine shoot heterogeneity clearly leads to visible and physiological imbalances that would impact negatively on grape and wine quality as well as production costs and should therefore be avoided on any terroir. / AFRIKAANSE OPSOMMING: In hierdie studie is die effek van heterogene lootontwikkeling in die lower van 'n ShirazlRichter 99 wingerd ondersoek. Vergelykings is getref tussen normale en onderontwikkelde lote in beskadude en blootgestelde lowers ten opsigte van hul vegetatiewe en reproduktiewe groeieienskappe, fisiologiese aktiwiteit en druifsamestelling en -gehalte. 'n Groter totale blaaroppervlak het by die normaalontwikkelde lote voorgekom as gevolg van die groter aantal sekondêre lote en groter primêre en sekondêre blare. Aangesien die blare van die normaal ontwikkelde lote fotosinteties meer aktief was as dié van onderontwikkelde lote, het die eersgenoemde lote groter hoeveelhede koolhidrate geproduseer en gestoor. Styselopberging het meer eweredig oor die lengte van die normale lote plaasgevind. Laasgenoemde lote was ook heelwat langer en dikker in deursnee as die onderontwikkelde lote. Die gunstiger totale blaaroppervlak per korrelmassa verhouding van die normale lote is duidelik weerspieêl in die groter trosse, terwyl die kleiner korrels (en dus die groter dop:pulp verhouding) op 'n groter potensiaal vir kleur- en fenolekstraksie tydens die wynbereidingsproses dui. Die kleiner trosse en kleiner aantal korrels per tros wat by die onderontwikkelde lote gevind is, dui op 'n wanbalans tussen die vegetatiewe en reproduktiewe groei van die loot wat tydens die vegetatiewe groeifase van die wingerdstok geïnisieer is en tydens die rypwordingsperiode voortgesit is. Die vreemde afwesigheid van enige statisties betekenisvolle verskille in druifsamestelling tussen die normale en onderontwikkelde lote dui daarop dat die verbindings wat vir die rypmaking van trosse op onderontwikkelde lote aangewend is, waarskynlik van ander wingerdorgane (bv. naasliggende lote, kordonarms, stam, wortels) as die spesifieke loot se blare afkomstig was. Die waarneming dat die korrelgroottes van normale en onderontwikkelde lote meer van mekaar verskil het in die beskadude as blootgestelde lowers, kan moontlik as bewys hiervoor dien. Die fotosintetiese aktiwiteit van beide loottipes was laer in die skaduryke lowers. Die koolhidrate wat deur normaalontwikkelde lote in skadu-Iowers geproduseer is, was oênskynlik onvoldoende vir die rypmaking van die loot self, die spesifieke loot se trosse, asook die trosse en lootweefsel van naasliggende onderontwikkelde lote. Hierdie bewerings word gerugsteun deur die laer vlakke van styselakkumulasie wat by die normale lote in beskadude lowers gevind is. Aangesien heterogene lootontwikkeling en -groei duidelike sigbare en fisiologiese wanbalanse in die wingerdstok tot gevolg het wat negatief op druifsamestelling, wyngehalte en produksiekoste inwerk, behoort dit in kommersiêle wingerde vermy te word.

Thesis (MSc)--University of Stellenbosch, 2004. / ENGLISH ABSTRACT: Arbuscular mycorrhizal (AM) C-costs in grapevines were investigated. Since both dormant vines and AM colonisation rely on stored C for initial growth, AM colonisation costs would therefore compete with plant growth for available C reserves. The aims of this study were to assess the host C economy during AM development and the subsequent C-costs of N and P uptake, as well as the effects of C costs on host growth. This was evaluated in two separate experiments; one assessing the symbiotic influence on the C costs of fungal establishment and nutritional benefits, whilst the other one evaluated the effects of the symbiosis on host growth and nutrient productivities. This study has shown that AM acts as a C sink, competing with the host for available C. Past work on the AM sink effect has focused mainly on the movement of photosynthetic C below ground to support the AM fungus. This however, does not take into account the effect that stored C will have on the C economy of the plant and symbiosis. The role of stored C becomes even more crucial when working with deciduous plants that rely on stored C for new growth at start of a growing season. It has been reported that stored C in AM plants is remobilized at the start of a growing season and then the C reserves are refilled towards the end of the season, when the plants enter dormancy. The initial costs of AM fungal colonisation were borne by the above-ground C reserves, at the expense of new growth in host plants. These costs were offset once the plateau phase was reached, and the depleted reserves started to refill. Once established, the active symbiosis imposed a considerable below ground C sink on host reserves. In spite of these costs, the improved P nutrition of AM roots was achieved with a more efficient C-use. This concurs with other findings, that of the belowground C allocated to AM roots, a greater part is used by AM respiration and a smaller part for P uptake. The C costs of the AM fungal phase of rapid development can be seen as negative to root growth and shoot development. These negative effects may continue for a period of time, even during the plateau phase of fungal development. Once the AM symbiosis is fully established, the host growth and development is then improved to a greater extent than in non-AM plants. From this study it can be concluded that AM growth directly competes with host development, but the symbionts revert to a beneficial partnership once it is fully established. / AFRIKAANSE OPSOMMING: Die C koste van arbuskulêre mikorisa (AM) in wingerdstokke is ondersoek. Beide rustende wingerdstokke en AM koloniseering is afhanklik van gestoorde C vir aanvanklike groei. AM kolonisering sou dus met plantgroei kompeteer vir beskikbare C reserwes. Die doelstellings van hierdie ondersoek was eerstens om die C-ekonomie van die gasheer tydens AM ontwikkeling en die gevolglike Ckostes van N en P opname te bepaal en tweedens sowel as die invloed van C veranderings op gasheergroei vas te stel. Hierdie is in twee afsonderlike eksperimente ondersoek: een om die simbiotiese invloed op die C-kostes van swam-vestiging en voedingsvoordele te bepaal, terwyl die ander die uitwerking van simbiose op gasheergroei en voedings doeltreffenheid evalueer het. Die ondersoek het bewys dat AM, as ‘n C-sink, kompeteer met die gasheer vir beskikbare C. Vorige werk oor die AM sink-effek het hoofsaaklik gefokus op die afwaartse beweging van fotosintetiese C om die AM-swam ondergronds te ondersteun. Die werk neem egter nie in ag wat die effek van gestoorde C op die C-ekonomie van die plant en simbiose sou wees nie. Die rol van gestoorde C is selfs nog meer belangrik wanneer met bladwisselende plante gewerk word, omdat sulke plante op gestoorde-C vir nuwe groei aan die begin van die groeiseisoen staatmaak. Dit is op rekord dat gestoorde C in bladwisselende plante by aanvang van die groeiseisoen gemobiliseer word en dat die C-reserwes teen die einde van die seisoen wanneer die plante rustyd nader, weer hervul word. Die aanvanklike kostes van AM kolonisering is deur die bogronds C-reserwes, ten koste van nuwe groei van die gasheerplante, gedra. Hierdie kostes herstel sodra die plato-fase bereik is, waar die uitgeputte reserwes begin hervul het. As die aktiewe simbiose eers gevestig is, sal dit as ‘n onderg P-voeding van AM wortels verkry wordrondse C-sink vir gasheer optree.Hierdie C verbruik word egter as doeltreffend beskou aangesien verbeterde. Dit is bekend dat ‘n groter deel van die ondergrondse C geallokeer word aan AM-wortels, deur middel van AM respirasie en P-opname. Die C-kostes van die AM-fungus tydens die fase van vinnige ontwikkeling, kan ‘n negatiewe effek op wortel- en lootontwikkeling hê. Hierdie negatiewe uitwerking kan vir ‘n tydperk voortdeur, selfs gedurende die plato-fase van fungi-ontwikkeling. Sodra die AM-simbiose volledig gevestig is, word gasheergroei en ontwikkeling tot ‘n groter mate verbeter as in plante sonder AM-fungi. Hierdie ondersoek het bewys dat AM groei direk met gasheerontwikkeling kompeteer, maar dat die simbiose ‘n voordelige vennootskap vorm sodra dit volledig gevestig is.