Thesis (MScAgric)--University of Stellenbosch, 2003. / ENGLISH ABSTRACT: An open air hydroponic production system (OHS) is based on classic hydroponic principles, with the difference that it lacks climatological control because the plants are not produced in greenhouses and are cultivated in the outside environment. In these systems the plant is provided with all the essential nutrients through the irrigation system, which is scheduled according to accurate measurements of the available soil water, in three to seven pulses a day. The rationale is that, by delivering nutrients each day, the mixes can be representative of what the plant actually requires for that specific phenological stage. The aim of this study was to monitor the usefulness and impact of OHS on table grape production within the framework of a case study. For this purpose, it was necessary that all factors involved in the development and growth of the plant should be studied and integrated in a multidisciplinary approach. Currently very limited information exists on basic guidelines for the effective implementation of these systems for table grape production, particularly with regard to local conditions. As a start, and to establish some guidelines and measurable parameters for the implementation of these systems, vegetative growth parameters were analysed within the framework of yield and fruit quality. The experiments were performed in a commercial vineyard in the Paarl region and the cultivars used were Dan ben Hannah (DBH) and Waltham Cross (WC). Relevant soil and climatic conditions, irrigation scheduling, fertiliser application, as well as cultivation practices, were taken into account. The soil maps provided information on the soil types identified in the blocks prior to the establishment of the two cultivars. The conventionally treated vines were irrigated and fertilised according to historical block data, and the OHS-treated vines according to programmes established by two different consultants. The experimental layout included a comparison of conventional cultivation methods and vines that had been switched over from conventional methods to OHS in the middle of 2000. All measurements within the different treatments were done at specific measuring points laid out statistically. Ten phenological stages were chosen to monitor the various aspects throughout the season for both cultivars treated conventionally and hydroponically. All relevant climatic parameters were collected for this specific production unit. The growth, fertility and quality indicators of these cultivars under the mentioned cultivation practices are discussed and established through quantitative analysis One of the aims, namely to show that established table grape vines could adapt from micro-irrigation to drip irrigation within two seasons in terms of root adaptation, was proven in this study. Initially the soil types were identified as Cartref, Clovellyand Glenrosa for both cultivars. The WC block contained an Avalon and the DBH a Westleigh soil type as well. The soil pits in all four treatments revealed the soil type to be a Tukulu form with differences in the clay content. Active roots developed underneath the drip lines for the OHS-treated cultivars, while the roots were still evenly distributed over the entire soil profile for the conventionally treated vines. Also, both cultivars adapted to OHS in terms of yield and production within two seasons, especially WC, which produced a higher yield in the 2001/2002 season than in the previous four seasons. DBH showed a strong vegetative reaction to OHS in terms of excessive vegetative growth, which had an indirect effect on fruit quality and bud fertility. A higher rate of bud mite infection in the OHS-treated vines also had a negative influence on bud fertility. The excessive vegetative growth was due to a rainy 2001/2002 growth season, in combination with the irrigation and fertiliser programmes. The irrigation and fertiliser programmes were changed from the 2000/2001 to the 2001/2002 season because of the change in consultants. As a result of this change, the OHS-treated vines were given very high nitrogen, phosphorus, potassium and micronutrient applications in the 2001/2002 season. The penetration of light in the canopy of the OHS-treated DBH was lower than in the conventionally treated DBH as a result of the above-mentioned factors, but the situation in Waltham Cross was the opposite. In the winter season of 2001, both OHS-treated cultivars were not fully adapted to the new system, as their pruning mass was lower than in the conventionally treated cultivars. No significant differences were determined for the winter cane starch content of both cultivars under conventional and OHS treatments. Effective fertiliser uptake proved to be suboptimal, especially in the case of calcium. Fruit analyses showed a lower calcium content in the OHS-treated fruit, which led to a poor skin cell structure and higher Botrytis infection during cold storage. The OHStreated cultivars showed more compact bunches, with an overall smaller rachis structure, which was another reason for the higher Botrytis infection during cold storage. However, the more compact rachis structure could not be explained on the basis of the elemental analyses. The OHS-treated DBH showed a more intense red berry colour, while the OHS-treated WC had a higher Fosscan Brix value. Both OHS-treated cultivars therefore ripened earlier than the conventional treatments. One of the advantages of the use of OHS in table grape production shown in this study was the ability to manipulate the phenology of the grapevine to provide fully ripened grapes a few days earlier than the conventionally treated grapes. This kind of advantage could be used to manipulate the production of table grapes for a better market window. Despite its limitations, this study concluded that the use of OHS for table grape production might be a useful tool for future production management, but that accurate management regarding irrigation and nutrient applications is a prerequisite. This will have to be developed systematically through experimentation to fully unlock the potential of the OHS management system for table grape production. This study provides a starting point for future research to elucidate these aspects and has clearly shown that even established vineyards can be switched to OHS in a relatively short period of time. It is envisaged that the advantages of this system, as long as the correct management protocols are in place, could have a positive effect on the production of high quality fruit for the international market. / AFRIKAANSE OPSOMMING: 'n Oop hidroponiese produksiestelsel (OHS) is gebaseer op klassieke hidroponiese beginsels, met die verskil dat OHS nie klimatologies beheer kan word nie. Die plante word nie in glashuise of tonnelsisteme verbou nie, maar wei onder buitelug toestande. Binne hierdie sisteme word die plante van alle noodsaaklike voedingstowwe deur die besproeiingstelsel voorsien. Hierdie voedingstowwe word in drie tot sewe pulse per dag volgens akkurate beskikbare grondwaterbepalings geskeduleer. Die rasionaal is dat, deur die daaglikse lewering van voedingstowwe, die mengsels verteenwoordigend is van die plant se behoefte vir 'n spesifieke fenologiese stadium. Die doel van die studie was om die bruikbaarheid en impak van OHS op tafeldruifproduksie binne die raamwerk van 'n gevallestudie te monitor. Alle faktore wat by die groei en ontwikkeling van die plant betrokke is, moet in 'n multidissiplinêre benadering bestudeer en geïntegreer word. Daar is tans slegs beperkte inligting oor die basiese riglyne en effektiewe implementering van dié sisteme vir tafeldruifproduksie wat spesifiek is vir plaaslike toestande. As 'n beginpunt, en om sekere riglyne en meetbare parameters vir die implementering van die sisteme te vestig, is vegetatiewe parameters binne die raamwerk van opbrengs en vrugkwaliteit geanaliseer. Alle eksperimente is in kommersiële wingerdblokke in die Paarl-omgewing uitgevoer. Die kultivars wat vir die studie gebruik is, is Dan ben Hannah (DBH) en Waltham Cross (WC). Alle relevante grond- en klimaatstoestande, sowel as besproeiingskedulering, bemestingprogramme en verbouingspraktyke is vir die projek in ag geneem. Ou grondkaarte het inligting ten opsigte van die verskillende grondtipes wat voor die vestiging van die verskillende kultivars geïdentifiseer is, verskaf. Die wingerde, onder konvensionele behandeling, is volgens historiese blokdata besproei en bemes, terwyl die wingerde onder OHS volgens geskeduleerde programme wat vanaf konsultante verkry is, besproei en bemes is. Die eksperimentele uitleg het 'n vergelyking van konvensionele verbouingsmetodes en wingerde wat in die middel van 2000 van konvensionele na OHS-verbouing oorgeskakel is, ingesluit. Alle metings en analises binne die verskillende behandelings het by spesifieke statistiesbepaalde punte plaasgevind. Tien fenologiese stadia is gekies om die verskillende aspekte vir beide kultivars onder konvensionele en OHSbehandeling gedurende die seisoen te monitor. Alle relevante klimaatsdata is vir die spesifieke produksie-eenheid aangevra. Alle groei-, vrugbaarheids- en kwaliteitsparameters van die kultivars onder die bogenoemde behandelingsmetodes is bespreek en gevolglik deur kwantitatiewe analises bepaal. Een van die doelwitte, naamlik om uit te vind of reeds gevestigde ouer wingerde in terme van wortelaanpassing binne twee seisoene kan aanpas van mikrobesproeiing na drup OHS, is deur die studie bevestig. Aanvanklik was die grondtipes geidentifiseer as die vorme, Cartref, Clovelly en Glenrosa vir beide kultivars, met 'n Avalon vorm adisioneel by WC, en 'n Westleigh vorm by die DBH. Grondprofiele het egter getoon dat die grondtipes vir al vier behandelings 'n Tukulu vorm is met verskillende klei inhoude. Aktiewe wortels het vir die OHS-behandelde wingerde onder die druppers ontwikkel, terwyl die wortels in die konvensionele behandeling steeds eweredig oor die hele grondprofiel versprei was. Beide kultivars het ook in terme van opbrengs en produksie binne twee seisoene ná die oorskakeling van die konvensionele behandeling na OHS aangepas. WC het in die 2001/2002 seisoen 'n hoër opbrengs gelewer as in die vorige vier seisoene. Afgesien van die goeie aanpasbaarheid, het DBH 'n sterk vegetatiewe groeireaksie ten opsigte van die OHS-behandeling getoon, wat 'n indirekte effek op vrugkwaliteit en oogvrugbaarheid geopenbaar het. 'n Hoër mate van knopmiet infeksie in die OHSbehandelde stokke kon ook 'n negatiewe bydrae tot oogvrugbaarheid gemaak het. Die sterk vegetatiewe groei kon aan die hoër reënval gedurende die 200112002 groeiseisoen, tesame met die besproeiings- en bemestingsprogramme, toegeskryf word. Die besproeiings- en bemestingsprogramme is verander van die 2000/2001 seisoen na die 2001/2002 seisoen weens die aanstelling van 'n ander konsultant. As gevolg van hierdie verandering het die OHS-behandelde stokke baie hoë toedienings van stikstof, fosfaat, kalium en mikroelemente in die 2001/2002 seisoen ontvang. Sonligpenetrasie in die wingerdlower van die OHS-behandelde DBH was laer as in die konvensionele behandeling as gevolg van die bogenoemde faktore. Die situasie vir WC was egter die teenoorgestelde. Tydens die winterseisoen van 2001 was beide oorgeskakelde kultivars nie ten volle by die nuwe OHS-behandeling aangepas nie, soos gesien kan word in die laer lootmassas in vergelyking met die konvensionele kultivars. Geen beduidende verskil is vir die hoeveelheid stysel in die winterlote van beide kultivars onder konvensionele en OHS-behandeling verkry nie. Effektiewe voedingstofopname was suboptimaal, veral betreffende kalsium. Vruganalises het 'n laer kalsiuminhoud in die OHS-behandelde druiwe getoon, wat aanleiding gegee het tot 'n swakker seistruktuur in die druiwedoppe en 'n hoor mate van Bottytis-infeksie gedurende koelopberging. Die OHS-behandelde kultivars het meer kompakte trosse getoon met kleiner trosraamwerke, wat ook aanleiding kon gegee het tot 'n hoër Botrytis-infeksie. Die kleiner trosraamwerke kon nie deur voedingstofanalises verklaar word nie. Die OHS-behandelde DBH het 'n hoër kleurintensiteit getoon, en die OHS-behandelde WC het 'n hoër suikerinhoud getoon ten opsigte van die konvensionele behandeling. Beide OHS-behandelde kultivars was dus vroeër ryp as die konvensionele behandelings. Een van die voordele van OHS wat uit die studie voortspruit, is die vermoë om wingerdfenologie te manipuleer om ryper druiwe vroeër in die seisoen te verkry. Hierdie tipe voordeel kan aangewend word om druiwe vir "n beter markvenster te produseer. Hierdie studie, tesame met al sy beperkinge, kom tot die gevolgtrekking dat die gebruik van OHS vir tafeldruifproduksie 'n nuttige instrument vir toekomstige produksiebestuur is, maar dat die optimale bestuur van besproeiing en bemesting as kritiese faktore beskou moet word. Hierdie faktore sal sistematies deur intensiewe navorsing ontwikkel moet word om die volle potensiaal van OHS te ontsluit, spesifiek vir tafeldruifproduksie. Hierdie studie kan as 'n beginpunt gebruik word vir toekomstige navorsing vir meer toegeligte verklarings van die bogenoemde aspekte, veral met die voordeel dat reeds gevestigde wingerde maklik en vinnig by OHS kan aanpas. Die voordele van OHS kan 'n groot positiewe invloed op die produksie van hoër kwaliteit tafeldruiwe vir die internasionale mark hê, mits die regte bestuursriglyne in plek is en toegepas word.

Thesis (MSc)--University of Stellenbosch, 2004. / ENGLISH ABSTRACT: Arbuscular mycorrhizal (AM) C-costs in grapevines were investigated. Since both dormant vines and AM colonisation rely on stored C for initial growth, AM colonisation costs would therefore compete with plant growth for available C reserves. The aims of this study were to assess the host C economy during AM development and the subsequent C-costs of N and P uptake, as well as the effects of C costs on host growth. This was evaluated in two separate experiments; one assessing the symbiotic influence on the C costs of fungal establishment and nutritional benefits, whilst the other one evaluated the effects of the symbiosis on host growth and nutrient productivities. This study has shown that AM acts as a C sink, competing with the host for available C. Past work on the AM sink effect has focused mainly on the movement of photosynthetic C below ground to support the AM fungus. This however, does not take into account the effect that stored C will have on the C economy of the plant and symbiosis. The role of stored C becomes even more crucial when working with deciduous plants that rely on stored C for new growth at start of a growing season. It has been reported that stored C in AM plants is remobilized at the start of a growing season and then the C reserves are refilled towards the end of the season, when the plants enter dormancy. The initial costs of AM fungal colonisation were borne by the above-ground C reserves, at the expense of new growth in host plants. These costs were offset once the plateau phase was reached, and the depleted reserves started to refill. Once established, the active symbiosis imposed a considerable below ground C sink on host reserves. In spite of these costs, the improved P nutrition of AM roots was achieved with a more efficient C-use. This concurs with other findings, that of the belowground C allocated to AM roots, a greater part is used by AM respiration and a smaller part for P uptake. The C costs of the AM fungal phase of rapid development can be seen as negative to root growth and shoot development. These negative effects may continue for a period of time, even during the plateau phase of fungal development. Once the AM symbiosis is fully established, the host growth and development is then improved to a greater extent than in non-AM plants. From this study it can be concluded that AM growth directly competes with host development, but the symbionts revert to a beneficial partnership once it is fully established. / AFRIKAANSE OPSOMMING: Die C koste van arbuskulêre mikorisa (AM) in wingerdstokke is ondersoek. Beide rustende wingerdstokke en AM koloniseering is afhanklik van gestoorde C vir aanvanklike groei. AM kolonisering sou dus met plantgroei kompeteer vir beskikbare C reserwes. Die doelstellings van hierdie ondersoek was eerstens om die C-ekonomie van die gasheer tydens AM ontwikkeling en die gevolglike Ckostes van N en P opname te bepaal en tweedens sowel as die invloed van C veranderings op gasheergroei vas te stel. Hierdie is in twee afsonderlike eksperimente ondersoek: een om die simbiotiese invloed op die C-kostes van swam-vestiging en voedingsvoordele te bepaal, terwyl die ander die uitwerking van simbiose op gasheergroei en voedings doeltreffenheid evalueer het. Die ondersoek het bewys dat AM, as ‘n C-sink, kompeteer met die gasheer vir beskikbare C. Vorige werk oor die AM sink-effek het hoofsaaklik gefokus op die afwaartse beweging van fotosintetiese C om die AM-swam ondergronds te ondersteun. Die werk neem egter nie in ag wat die effek van gestoorde C op die C-ekonomie van die plant en simbiose sou wees nie. Die rol van gestoorde C is selfs nog meer belangrik wanneer met bladwisselende plante gewerk word, omdat sulke plante op gestoorde-C vir nuwe groei aan die begin van die groeiseisoen staatmaak. Dit is op rekord dat gestoorde C in bladwisselende plante by aanvang van die groeiseisoen gemobiliseer word en dat die C-reserwes teen die einde van die seisoen wanneer die plante rustyd nader, weer hervul word. Die aanvanklike kostes van AM kolonisering is deur die bogronds C-reserwes, ten koste van nuwe groei van die gasheerplante, gedra. Hierdie kostes herstel sodra die plato-fase bereik is, waar die uitgeputte reserwes begin hervul het. As die aktiewe simbiose eers gevestig is, sal dit as ‘n onderg P-voeding van AM wortels verkry wordrondse C-sink vir gasheer optree.Hierdie C verbruik word egter as doeltreffend beskou aangesien verbeterde. Dit is bekend dat ‘n groter deel van die ondergrondse C geallokeer word aan AM-wortels, deur middel van AM respirasie en P-opname. Die C-kostes van die AM-fungus tydens die fase van vinnige ontwikkeling, kan ‘n negatiewe effek op wortel- en lootontwikkeling hê. Hierdie negatiewe uitwerking kan vir ‘n tydperk voortdeur, selfs gedurende die plato-fase van fungi-ontwikkeling. Sodra die AM-simbiose volledig gevestig is, word gasheergroei en ontwikkeling tot ‘n groter mate verbeter as in plante sonder AM-fungi. Hierdie ondersoek het bewys dat AM groei direk met gasheerontwikkeling kompeteer, maar dat die simbiose ‘n voordelige vennootskap vorm sodra dit volledig gevestig is.

Thesis (MScAgric (Viticulture and Oenology))--University of Stellenbosch, 2010. / ENGLISH ABSTRACT: The table grape industry employ a wide range of viticultural management practices in order to produce the high quality grapes demanded by the export market. A common contributor to degrading the quality of table grapes is the occurrence of berry split, which not only has an unattractive visual effect, but also increases the berries’ susceptibility to infection by spoilage organisms. A number of environmental conditions such as rainfall and humidity, and/or agricultural practices, such as irrigation, and high density canopies, can lead to higher plant cell water content. This in turn, can increase the potential of berry split to occur. To date, the main method of berry split prevention has been the management of plant water status by; (i) regulating irrigation withdrawal times, and (ii) covering of canopies if rainfall is predicted prior to harvest. The aim of this study was to determine the effect that irrigation frequency, as induced by irrigation withdrawals; as well as boron (B) and calcium (Ca) treatments, applied as bunch directed sprays, have on pre- and post-harvest berry split. To this end, a newly released late ripening, white seedless cultivar, SoutherngrapeOne was chosen as a model cultivar as it has a high susceptibility to berry split. SoutherngrapeOne vines were subsequently subjected to a range of irrigation frequencies based on typical irrigation scheduling used in the table grape industry, which comprised of a low, medium and high frequency. The low frequency was duplicated in order to demonstrate the effect that a heavy irrigation, just before harvest may have on berry split. These treatments were further subdivided to investigate the effect that B and Ca may have on berry split. For the B treatment, four Solubor1 bunch directed sprays were applied from 8mm berry size to véraison. The Ca treatment consisted of Stopit R 2 and Caltrac R 3 bunch directed sprays applied over the same period. In addition, a combination of the B and Ca treatment were applied to investigate any possible interaction. To account for the effect of water as solvent in the B and Ca treatments, and the spraying effect, pure water as treatment was also evaluated. Control vines received no sprays. The applied irrigation treatments resulted in different plant water status conditions. Separate applications of B and Ca treatments resulted in a decrease of B and Ca content in the flesh respectively. The control and combination treatment, of B and Ca resulted in the same of B and Ca content in the flesh. Furthermore, none of the applied treatments resulted in an increase of either B or Ca content in the berry skin. It was found that the medium frequency irrigation resulted in the best irrigation strategy to prevent pre-harvest berry split. Surprisingly, all the subtreatments: B, Ca, and combination of B and Ca, resulted in an increased incidence of pre-harvest berry split when compared to the control group for the 2006/07 season. However, in the 2007/08 season only the B treatment resulted in an increase of pre-harvest berry split. Concerning post-cold-storage physiological disorders, Ca treatments appear to have reduced berry drop, but increased decay. In the 2006/07 season, the B treatment resulted in reduced post-cold-storage berry split, whereas Btreatment in the 2007/08 season had no effect. Both B and Ca should be considered as having the potential to increase the appearance of hairline cracking. Calcium treatment also led to an increase in decay which may have been as a result of the splitting it contributed to. Low frequency irrigation recieving irrigation before harvest was found to result in browner stems. Low irrigation frequencies decreased the cell size of the berry skin. The Ca treatment gave rise to thicker (weaker) cell walls, this morphological change may be responsible for the physiological disorders it caused. From these findings, it can be deduced that poorly managed irrigation, together with unnecessary application of B and/or Ca may result in an increase of berry split and other physiological disorders, with subsequent financial losses for the producer. / AFRIKAANSE OPSOMMING; Die tafeldruifindustrie maak gebruik van ’n wye reeks wingerdkundige praktyke ten einde die hoë gehalte druiwe te produseer wat die uitvoermark vereis. Korrelbars is ’n algemene verskynsel wat afbreek maak tot die gehalte van tafeldruiwe. Behalwe dat voorkoms van die druiwe benaadeel word, verhoog dit ook in vatbaarheid vir infeksie deur verrottingsveroorsakende swamme. Hoë reënval en humiditeit, sowel as wingerdkundige praktyke soos besproeiing en hoë lowerdigtheid, wat kan lei tot verhoogde waterstatus in plante, kan lei tot ’n toename in korrelbars. Daar word hoofsaaklik van twee metodes gebruik gemaak om korrelbars te beheer, naamlik die bestuur van plantwaterstatus deur; (i) beheer van besproeiingsontrekkingstye en (ii) bedekking van lowers indien reën voorspel word voor oestyd. Die doel van hierdie studie was om vas te stel wat die invloed van besproeiings frekwensies sowel as trosgerigte boor (B) en kalsium (Ca), spuitbehandelings, op voor- en na-oes korrelbars het. Die onlangs vrygestelde laat rypwordende, wit, pitlose kultivar, SoutherngrapeOne is gebruik, aangesien dit hoogs gevoelig is vir korrelbars. Stokke is aan verskillende besproeiings intervalle, soos tipies gebruiklik in die tafeldruifindustrie, blootgestel. Hierdie intervalle bestaan uit n’ lae, medium en hoë besproeiings frekwensie. Die lae besproeiings frekwensie is herhaal ten einde die invloed van besproeiing net voor oestyd op korrelbars te ondersoek. Die invloed van B- en Ca-behandeling op korrelbars is ook ondersoek. Vir die B-behandeling is vier Solubor1 trosgerigte spuite aangewend vanaf 8mm korrelgrootte tot deurslaan. Vir die Ca-behandeling is Stopit R 2 en Caltrac R 3 as trosgerigte spuite oor dieselfde tyd toegedien. Kombinasiebehandelings is ook aangewend om enige interaksie tussen B en Ca te ondersoek. Waterbehandelings is ook toegedien om die invloed van water as oplosmiddel van B- en Ca-behandelings sowel as die spuit-effek te ondersoek. Kontrole stokke is ook ingesluit en het geen spuitebehandeling ontvang nie. Die besproeiingsbehandelings het verskillende plantwater toestande tot gevolg gehad, B- en Ca-behandelings het gelei tot ’n afname in B- en Cainhoud in die vleis onderskeidelik. Die kombinasie en kontrole behandelings het eenderse hoeveelhede B en Ca in die vleis tot gevolg gehad. Geen van die aangewende behandelings gelei tot ’n toename in B- en Ca-inhoud in die dop nie. Die resultate toon dat medium besproeiings frekwensie die beste besproeiingsstrategie is om voor-oes korrelbars te voorkom. In vergelyking met die kontrole-behandeling in 2006/07, het B, Ca en die kombinasie van B en Ca, ’n toename in voor-oes korrelbars tot gevolg gehad. In die 2007/08 seisoen het slegs die B-toediening egter tot ’n toename in voor-oes korrelbars gelei. Kalsium behandelings het ’n afname in los-korrels, maar ’n verhoging in korrelbars tot gevolg gehad. In 2006/07, het B-toediening tot ’n afname in korrelbars na koelopberging gelei, maar in die 2007/08 seisoen het dit geen effek gehad nie. Beide B- en Ca-toediening het die potensiaal om haarlyn barste te veroorsaak. Kalsium toediening het bederf verhoog wat moontlik aan die hoër bars wat dit induseer toegeskryf kan word. Lae besproeiings frekwensie, het bruiner stingels veroorsaak, en ook gelei tot ’n afname van selgrootte in die dop. Die Ca-toediening het aanleiding gegee tot dikker selwande in die dop. Hierdie anatomiese veranderinge kan moontlik die rede wees vir die verhoging in fisiologise afwykings. Van hierdie bevindinge kan ons aflei dat swak bestuur van besproeiing, sowel as die onnodige aanwending van B en/of Ca, kan aanleiding gee tot ’n toename in korrelbars en ander fisiologiese afwykings, en dus finansiële verliese vir die produsent inhou.