Thesis (LLM. (Philosophy of Environmental Law and Management)) -- University of Limpopo, 2014 / Human well-being and progress toward sustainable development are vitally dependent upon improving the management of the Earth’s ecosystems to ensure their conservation and sustainable use. But while demands for ecosystem services such as food and clean water are growing, human actions are at the same time diminishing the capability of many ecosystems to meet these demands. This study explores the legal framework for the protection of grassland in South Africa. In particular, the study looks at the legal protection introduced to protect grassland in Haenertsburg area in South Africa. This study argues that Grassland is vital to the ecosystem, and plays a pivotal role in maintaining and providing life for many plant and animal species. The grassland is also important in our daily lives and is one of the most threatened biomes in South Africa. The study concludes by arguing that more regulatory measures are needed in order to protect the grasslands as threaten species.

The increasing human population, industrialization, urbanisation and climate change challenges have resulted in an increased demand for already scarce water resources. This has left the agricultural sector with less water for production. Sustainable water management strategies would therefore require accurate determination of water-use. In agriculture, water-use can best be determined from total evaporation which is the loss of water from soil and vegetation to the atmosphere. Accurate quantification of total evaporation from vegetation would require a thorough understanding of water transport processes between vegetation and the atmosphere, especially in a water-scarce country like South Africa. Several methods for estimating total evaporation have been developed and are in use today. Some of the common methods used today are: the Bowen ratio energy balance, eddy covariance, scintillometry, flux variance and surface renewal. However, various methods have advantages and disadvantages. Considerations include the cost of equipment and level of skill required for use of some of the methods. A number of methods involve indirect or direct estimation of sensible heat flux then calculating latent energy flux and hence total evaporation as a residual of the shortened energy balance equation. The main objective of this study is to determine the effects of grassland management practices on the energy balance components as well as on the surface radiation balance. Eddy covariance and surface renewal methods were employed to investigate the effects of grassland management practices (mowing and burning) on the micrometeorology of naturally occurring grassland. A 4.5-ha grassland site (Ukulinga, Pietermaritzburg, South Africa) was divided into two halves: one area was initially mowed (cut-grass site) to a height of 0.1 m while the other was not mowed (tall-grass site). The tall-grass site was later treated by burning and hence referred to as the burnt-grass site. Two eddy covariance systems were deployed, one at each of the cut-grass and the tall-grass sites. The systems each comprised a three-dimensional sonic anemometer to measure high frequency sonic temperature, orthogonal wind speeds and directions and the eddy covariance sensible heat flux (W m-2). Latent energy flux, from which total evaporation was then determined, was calculated as a residual from the shortened energy balance equation from measurements of sensible heat flux, net irradiance and soil heat flux assuming closure is met. Other microclimatic measurements of soil water content, soil temperature, surface reflection coefficient and reflected solar irradiance were performed, the latter with a four-component net radiometer. An automatic weather station was also set up at the research site for continuous measurements of solar irradiance, air temperature, relative humidity, wind speed and direction and rainfall. Water vapour pressure and grass reference evaporation were also determined online. Energy fluxes from the tall-grass site were measured from March to June 2008. Greater total evaporation rates (2.27 mm day-1) were observed at the beginning of the experiment (March). As winter approached most of the energy balance components showed a constant decreasing trend and the average total evaporation rates for May and June were 1.03 and 0.62 mm day-1, respectively. The tall-grass site had consistently lower soil temperatures that changed diurnally when compared to the cut-grass site. The soil water content at both sites showed no significant differences. Most of the energy balance components were similar between the two sites and changed diurnally. Although there were small differences observed between other energy balance components, for example, latent energy flux was slightly greater for the tall-grass site than for the cut-grass site. The tall-grass site had more basal cover and this may have contributed to the differences in temperature regimes observed between the two sites. However, the plants growing at the cut-grass site showed more vigour than the ones at the tall-grass site as spring approached. Burning of a mixed grassland surface caused significant changes to most of the optical properties and energy fluxes of the surface. Following burning, the soil temperature was elevated to noticeable levels due to removal of basal cover by burning. The surface reflection coefficient measured before and after the burn also presented a remarkable change. The surface reflection coefficient was significantly reduced after the burn but a progressive increase was observed as the burnt grass recovered after the spell of spring rains. The energy fluxes: net irradiance, latent energy flux and soil heat flux also increased following the burn but the latent energy flux was reduced as transpiration was effectively eliminated by the burning of all actively transpiring leaves. As a result, the main process that contributed towards latent energy flux was soil evaporation. An ideal surface renewal analysis model based on two air temperature structure functions was used to estimate sensible heat flux over natural grassland treated by mowing. Two air temperature lag times r (0.4 and 0.8 s) were used when computing the air temperature structure functions online. The surface renewal sensible heat fluxes were computed using an iteration process in Excel. The fluxes, obtained using an iterative procedure, were calibrated to determine the surface renewal weighting factor (a) and then validated against the eddy covariance method using different data sets for unstable conditions during 2008. The latent energy flux was computed as a residual from the shortened energy balance equation. The surface renewal weighting factor was determined for each of the two heights and two lag times for each measurement height (z) above the soil surface. The a values obtained during the surface renewal calibration period (day of year 223 to 242, 2008) ranged from 1.90 to 2.26 for measurement height 0.7 m and r = 0.4 and 0.8 s. For a measurement height of 1.2 m and r = 0.4 and 0.8 s, a values of 0.71 and 1.01 were obtained, respectively. Good agreement between surface renewal sensible heat flux and eddy covariance sensible heat flux was obtained at a height of 1.2 m using a = 0.71 and a lag time of 0.4 s. Total evaporation for the surface renewal method was compared against the eddy covariance method. The surface renewal method, for a height of 1.2 m and a lag time of 0.4 s, yielded 1.67 mm while the eddy covariance method yielded 1.57 mm for a typical cloudless day. For the same day for a measurement height of 1.2 m and a lag time of 0.8 s, eddy covariance and surface renewal methods yielded 1.57 and 1.10 mm, respectively. For a lag time of 0.4 s, the surface renewal method overestimated total evaporation by 0.10 mm while for a lag time of 0.8 s, the total evaporation was underestimated by 0.47 mm. As a result, the surface renewal method performed better for z = 1.2 m and a lag time of 0.4 s. The eddy covariance method gave reliable sensible heat fluxes throughout the experiment and this allowed a comparison of fluxes across all treatment areas to be achieved. The short-term analysis of the surface renewal method also gave reliable energy fluxes after calibration. Compared to the eddy covariance method, the surface renewal method is more attractive in the sense that it is easy to operate and use and it is relatively cheap. However, the surface renewal method requires calibration and validation against a standard method such as the eddy covariance method. This study showed that grassland management practices had a considerable effect on surface radiation and energy balance of the mowed and burnt treatment sites. Total evaporation was mainly controlled by the available energy flux, rainfall and grassland surface structure. High total evaporation values were observed during summer when net irradiance was at its highest and grass growth at its peak. Low total evaporation values were observed in winter (dry atmospheric conditions) when net irradiance was at its lowest and most vegetation was dormant. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2011.

Fire, natural or of anthropologic origin is a recurrent phenomenon in South African mesic grasslands. The species composition of these grasslands is sensitive to fire frequency and there exists a permanent fire-species relationship syndrome. The shift follows a general trend where, in the absence of fire for longer periods, the native grass species, possibly the most economically important grass species, give space to relatively less desirable and invasive species accompanied by significant decline in basal cover. Though much is known as to how the floristic composition of the grassland changes in response to fire, the underlying mechanisms responsible for changes in plant vigour and species composition are not fully understood. There exists limited information on germination requirements of the local flora and how recruitment of the species is influenced by fire, fire-related factors and the interaction of these factors among themselves is virtually unknown. Since the discovery by DE LANGE and BOUCHER (1990), plant-derived smoke is being widely studied as an important germination cue and the theme has been the subject of intense experimental research and theoretical work. Germination studies conducted on South African Fynbos, Californian Chaparral, and Australian species have illustrated the widespread ability of plant-derived smoke in promoting germination of species from both fire and non-fire prone habitats. Studies have also showed that plant-derived smoke plays a significant role in vegetative growth of many plants including horticultural and agricultural crops, though mechanisms of smoke action in enhancing germination and promoting plant growth are still under active research. This study sought to gain insight into the role of fire and fire-related cues and other related factors on germination and seedling growth of key grass species from fire-prone grassland in South Africa. In this study, various investigations were conducted on different aspects related to smoke-induced seed germination. Furthermore, in depth examinations were conducted on the effects of fire-related factors such as smoke, heat, soil and nutrients on seed germination and seedling growth of representative key mesic grassland species from South Africa. Laboratory-based germination and vigour experiments were conducted using smoke solutions. The aim was to examine if the strong fire-species relationship syndrome we encounter in post-fire mesic grassland in South Africa is related to the effect of plant-derived smoke on germination and seedling growth. In addition, the interaction of plant-derived smoke solutions with temperature was examined by incubating seeds at a range of temperatures. Treating seeds with smoke-water and butenolide, the germination rate (GR) and final germination percentage (FGP) were greater in 3 of the 6 species. Themeda triandra Forssk. and Tristachya leucothrix Trin. ex Nees showed the greatest response, with final germination increased from 43% to 67% and 35% to 63% respectively. These smoke solutions have also significantly enhanced germination in Eragrostis tef (Zucc.) Trotter from 62% to 80%. The degree of responsiveness varied from species to species and across different temperatures. Findings from this study suggested that plant-derived smoke and its interaction with temperature significantly influence the germination and seedling growth of the South African mesic grassland species, which can further impact on the grassland composition. To characterise the role of plant-derived smoke on certain economically important seed traits, the effect of smoke-water and a smoke-isolated butenolide on seed germination and seedling growth of Eragrostis tef (grass species which has moderately good tolerance to east African drought) was investigated at a range of temperatures, light conditions and osmotic potentials. Smoke-water (1:500 v/v) and butenolide (10ˉ⁸ M) treatment of the seeds increased percentage germination, seedling vigour and imbibition from high to low osmotic potential. These results suggested that smoke solutions have characteristics with overriding effects toppling stresses exerted from high temperature and low osmotic potential, thereby widening the temperature and moisture zone over which germination can occur. Furthermore, these findings suggested that the mode of smoke action in promoting seed germination is likely to be linked to the role of smoke in facilitating water uptake by seeds (imbibition). A simple and rapid bioassay was implemented to detect the germination activity of extracts from soils in pre/post-burn status. Soil samples taken from burnt, unburnt and adjacent plots at depths of 0-2, 2-4, 4-6 and 6-8 cm before and after burning mesic grassland in South Africa were analysed for germination activity over an eight-week period. Soil samples were extracted using dichloromethane and bioassayed using Grand Rapids lettuce (Lactuca sativa L.) achenes. The Grand Rapids lettuce seeds exhibited several-fold greater germination percentages when treated with extracts from burnt soil compared to the other plots. The magnitude of such an activity declined through time since burn. The Grand Rapids lettuce seeds also showed significantly greater germination percentage when treated with unburnt soil extracts compared to the control (distilled water) which indicates the existence of other factors controlling germination in unburnt soil. Germination percentage was negatively influenced in the adjacent plots which reflected the inhibitory role of smoke on adjacent plots neighbouring the burn. Findings from this study suggested that the germination activity of a burning plant-material (smoke) indeed diffuses into the soil and its persistence declines with time. Furthermore, the findings implied that fire and smoke may significantly influence the germination of the soil seed bank of mesic grassland in South Africa. The effect of smoke solutions of various concentrations and their interaction with soil macronutrients NPK in influencing seedling growth of three selected grass species namely, Eragrostis curvula, Panicum maximum and Themeda triandra was investigated. These grass species were selected on the basis of the contrasting response they previously showed to smoke treatments. Results showed that the interaction between species, treatment and nutrients for seedling vigour index was highly significant (P < 0.001). In the absence of NPK elements, the smoke solutions generally enhanced seedling growth in Themeda triandra, while suppressing seedling vigour indices of Eragrostis curvula and Panicum maximum. It is ecologically significant that not only smoke but also its interaction with soil macronutrients appears to be important in structuring the post-fire regeneration and colonisation processes. To examine the effect of fire-associated cues of smoke and high temperature on germination and seedling emergence from the mesic grassland soil seed bank in South Africa, and disentangle responsible factors, the effect of heat and smoke-water on seedling emergence and the resultant effect on biomass production was examined in a greenhouse tray experiment over 90 days. Soil seed bank samples obtained from 0-5 cm top soil were treated with smoke-water solution (SW 1:500 v/v), heat (H = 100 °C for 15 min) and heat + smoke-water (H = 100 °C for 15 min + SW = 1:500 v/v) combined. Significantly, the highest number of seedlings emerged from soil seed bank samples treated with heat + smoke-water combined followed by smoke-water alone. Heat + smoke-water treatment of soil seed bank samples also significantly enhanced the rate of seedling emergence and total dry biomass production. Heat treatment by itself was not statistically different from the control in many respects. Generally, these results suggest that both heat and smoke are important germination cues and play a curial role on germination and seedling emergence from the mesic grassland soil seed bank. A greater number of fire-loving Themeda triandra seedlings emerged from soils treated with smoke, though this was not statistically significant. Seedling emergence indicators showed that seedling emergence was favoured more by smoke and smoke + heat treatments compared to the control. Seedling growth indicators also showed that soil samples treated with smoke or smoke + heat combined produced significantly higher biomass than untreated samples. In general, findings from this comprehensive study suggest that plant-derived smoke and its interaction with temperature (heat) and soil nutrients significantly influences seed germination and seedling growth of South African mesic grassland species differently, this can further influence the grassland composition. The association of certain fire-climax species with the re-occurrence of fire can partly be explained due to the association of such species with fire-induced changes in the habitat. Post-fire conditions of low nutrient (NPK) availability, presence of high heat and smoke cues in the habitat may favour the competitive ability of native short and smoke-responsive species such as Themeda triandra and Tristachya leucothrix over those characteristic species of fertile habitats such as Eragrostis curvula and other non-smoke-responsive species. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

The effects of fire and mechanical clearing were investigated for their potential in assisting with the eradication of Chromolaena odorata (previously Eupatorium odoratum). The study was divided into two focus areas, the first focused on mechanical clearing of dense stands of C. odorata on three sites and the second focused on the long term influences of a single burn on C. odorata plants in the different size categories. For mechanical clearing, two key issues were investigated; namely whether this type of clearing procedure was effective in dense C. odorata stands and whether rehabilitation was necessary in these cleared areas. The study was conducted from July 2002 to June 2004. The area was subject to a severe drought throughout the duration of the study. The severe drought had a large influence on the result in both focus areas. A bulldozer was found to be a very effective at clearing dense C. odorata stands. Results from the mechanical clearing study showed that there was still a large viable grass seed population in the areas that had been covered by a dense stand of C. odorata plants for over three years. Thus, indigenous plants were able to re-colonize the area after removal of C. odorata without human intervention, even thought the area was experiencing a severe drought. The density of C. odorata seedlings emerging in the cleared areas was far lower than expected. The C. odorata density in the permanent plots, for seedlings that germinated in the first season after clearing (SeptemberOctober 2002), was only 0.25,0.03 and 0.72 per 5 m2 in the three sites respectively by the end of the study in June 2004. For the C. odorata seedlings that germinated in the second season (September-October 2003) the density was, 0.5, 0.56 and 1.06 per 5 m2 in the three sites respectively by the end of the study in June 2004. It was suspected that the drought influenced seed germination. Unfortunately the number of C. odorata seedlings was so low, that no significant relationship could be found between grass and C. odorata seedling density. By the end of the study the grass fuel mass in all the rehabilitated sites was already over 3000 kg ha-1, even though the area was experiencing a severe drought. This grass fuel load, when burnt, will assist land managers in controlling C. odorata plants, especially seedlings. Very few other alien invasive plant species emerged in the cleared areas. At the Mhlosinga site, Senna pendula made up less than one percent of the herbaceous species composition and only a single Ricinus communis plant was recorded. No alien plant species were recorded on the other two sites. Results from the burning trials revealed that plants in all the size categories were affected by fire. Greater fuel masses and fire intensities were required to kill larger C. odorata plants relative to smaller ones. Fire was found to be very effective at eliminating small and medium size C. odorata plants. Fire applied as a once off treatment had a significant long-term effect on the C. odorata population. The following fuel loads were required to achieve 80% mortality in this 11 study: for small plants a fuel load of over 4000 kg ha-I, for medium plants a fuel load over 4200 kg ha-I and for large plants a fuel load over 4600 kg ha-I. Little difference could be detected between a head or a back burn, as both fire types had their own advantages and disadvantages. Although some of the C. odorata plants in the burnt plots had not perished by the time of the first investigation, following the burn (February 2003), by the time of the second investigation (June 2004), many of these plants had eventually succumbed. These results highlighted the fact that plants which are damaged by fire were more likely to persish during an extended droughts period, than plants which were not subjected to fire. Results from the control plots, in the burning trials, for medium and large plants showed dramatic increases in density over time. Tagged individuals from the control plots did reveal that some of the medium and large plants did die during the drought, although the amount was negligible when compared to the number of new plants growing into the new size categories. A large proportion of the small plants in the control plots also survived the drought with many of them even growing into the medium category. The difference between the control plots and the burnt plots was obvious and significant, especially once the fuel mass exceeded 3783 kg ha-I. Results from this study show that fire can be used as a very effective tool in assisting land managers to control C. odorata in open savanna bushveld. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.

The KwaZulu-Natal Drakensberg, South Africa, is predominantly a grassland ecosystem maintained by fire. The effect of the current burning regime on invertebrate and flowering forb diversity in this ecosystem is poorly understood. The overall aim ofthis study was to contribute towards the development of an effective burning regime for the KwaZulu-Natal Drakensberg that will conserve invertebrates and indigenous forbs, two major components of biodiversity. The objectives were to examine the effect of fire and fire frequency on flowering forb and invertebrate species diversity, to determine whether fire frequency, time since last burn or locality were influencing species composition, and to identify potential biodiversity indicators that reflect overall species richness for use in monitoring of invertebrates and forbs. Sampling took place in March, September and November of 2002 at Giants Castle Game Reserve. Invertebrates were sampled using sweep netting and targeted netting along transects, yellow pan traps and soil quadrats. Invertebrate taxa sampled were ants (Formicidae), butterflies (Lepidoptera), grasshoppers (Orthoptera), leafboppers (Cicadellidae), bees (Apoidea), bee flies (Bombyliidae), hover flies (Syrphidae), robber flies (Asilidae), spiders (Araneae), earthworms (Oligochaeta) and millipedes (Diploda). These were identified to species level with the assistance of taxon experts. Flowering forbs were sampled using five replicates of five by five metre quadrats randomly placed in each site. Overall flowering forb and invertebrate species diversity was higher in grasslands that were burnt for two consecutive years in 2001 and 2002 than in grasslands that were not burnt during those two years. Frequently (annual) and intermediately (biennial) burnt grasslands had significantly higher invertebrate and flowering forb diversity than infrequently (five years without burning) burnt grasslands. This, together with the fact that grasslands burnt during the year of sampling had higher species richness than grasslands burnt two and five years previously suggests that invertebrates and forbs are generally resilient to fire and many forb species appear to be stimulated by fire. However, each burn frequency had its own suite of unique flowering forb and invertebrate species. Invertebrate communities were influenced mostly by locality and the length of time past since the last fire and flowering forb communities were influenced mostly by the length oftime past since the last fire. Fire frequency had the least influence on both invertebrate and forb communities. Ecological succession occurred after each fire in the invertebrate communities but forb communities appear to need more than five years without fire for ecological succession to occur. The findings of this study therefore suggest that using a combination of three fire frequencies would result in patches of grassland in various stages of ecological succession, and would conserve species unique to each burning frequency, and would therefore conserve maximum diversity. Flowering forb species richness and certain invertebrate taxa (ants, leafboppers, spiders and bees) have the potential to act as indicators of overall invertebrate species richness for use in monitoring programmes. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.