The purpose of this thesis is to understand and analyse the issues of grey water generation and disposal in peri-urban areas of Dar es Salaam in Tanzania and to propose a suitable solution to which includes designing a treatment system for grey water (water from shower, washing and kitchen). The purification will eliminate the sanitation issues that are brought by exposing untreated grey water to the environment as well save freshwater. This will be implemented with regard to local conditions in Dar es Salaam, Tanzania. Water-borne and vector-borne diseases are mainly brought by untreated grey water, which sadly enough causes thousands of death cases every year in Tanzania. The year between 2004- 2005, 154551 cases of dysentery were reported with 170 deaths. It was also reported, 863488 cases of typhoid with 1167 deaths and last but not least 12923 cases of cholera from with 350 deaths. Deaths that were caused by diarrhoea were also reported, whereby, 90 % of them are children. According to the interviews that were conducted, people would not want to recycle the grey water for example for washing due to socio-cultural context where water is considered dirty even after treatment. They had difficulties in believing that the same dirty grey water can be purified and reused again for various domestic activities. The conducted characterisation of grey water showed the tested grey water has relatively high levels of BOD, basic pH, higher than average values of TSS, relatively low levels of DO, increased salinity and average level of FC. The analyses were performed on the most polluted grey water from every source. The volume of household produced grey water varies strongly, depending on socio-economic situation of the household and type of access to water supply. From estimation, the volume varies approximately between 60 -160 l/day and household for a low income household. Living wall was proposed as grey water treatment system, because of its suitability to the peri-urban settlement and tropical climate. The system consists of concrete boxes placed over each other, filled with inner porous material and planted with plants. The system purifies grey water as it percolates through it and at the same time provides a vertical space for growing plants. The plants for the treatment system were chosen to Typha latifolia and Scirpus species. The plants are growing in Tanzania and are being used for wastewater treatment purposes. The plants are tolerant to increased salinity and can grow in alkaline soil. The cleaning effectiveness of the system was not tested, but only roughly estimated with help of values from a similar treatment system build in Gotland, Sweden. Hence, the reuse of grey water in this geographical location suits best for irrigation in rural and peri-urban areas with potential for agriculture as well as groundwater recharge in the peri-urban and urban areas. Recharging groundwater with treated grey water has a potential to be observed as an indirect and long term reuse of the water as it reconnects in the hydrological cycle.

The purpose of this thesis is to develop a working methodology to color a grey scale image. This thesis is based on approach of using a colored reference image. Coloring grey scale images has no exact solution till date and all available methods are based on approximation. This technique of using a color reference image for approximating color information in grey scale image is among most modern techniques.Method developed here in this paper is better than existing methods of approximation of color information addition in grey scale images in brightness, sharpness, color shade gradients and distribution of colors over objects.Color and grey scale images are analyzed for statistical and textural features. This analysis is done only on basis of luminance value in images. These features are then segmented and segments of color and grey scale images are mapped on basis of distances of segments from origin. Then chromatic values are transferred between these matched segments from color image to grey scale image.Technique proposed in this paper uses better mechanism of mapping clusters and mapping colors between segments, resulting in notable improvement in existing techniques in this category.

<p>The purpose of this thesis is to understand and analyse the issues of grey water generation and disposal in peri-urban areas of Dar es Salaam in Tanzania and to propose a suitable solution to which includes designing a treatment system for grey water (water from shower, washing and kitchen). The purification will eliminate the sanitation issues that are brought by exposing untreated grey water to the environment as well save freshwater. This will be implemented with regard to local conditions in Dar es Salaam, Tanzania.</p><p>Water-borne and vector-borne diseases are mainly brought by untreated grey water, which sadly enough causes thousands of death cases every year in Tanzania. The year between 2004- 2005, 154551 cases of dysentery were reported with 170 deaths. It was also reported, 863488 cases of typhoid with 1167 deaths and last but not least 12923 cases of cholera from with 350 deaths. Deaths that were caused by diarrhoea were also reported, whereby, 90 % of them are children.</p><p>According to the interviews that were conducted, people would not want to recycle the grey water for example for washing due to socio-cultural context where water is considered dirty even after treatment. They had difficulties in believing that the same dirty grey water can be purified and reused again for various domestic activities.</p><p>The conducted characterisation of grey water showed the tested grey water has relatively high levels of BOD, basic pH, higher than average values of TSS, relatively low levels of DO, increased salinity and average level of FC. The analyses were performed on the most polluted grey water from every source. The volume of household produced grey water varies strongly, depending on socio-economic situation of the household and type of access to water supply. From estimation, the volume varies approximately between 60 -160 l/day and household for a low income household.</p><p>Living wall was proposed as grey water treatment system, because of its suitability to the peri-urban settlement and tropical climate. The system consists of concrete boxes placed over each other, filled with inner porous material and planted with plants. The system purifies grey water as it percolates through it and at the same time provides a vertical space for growing plants.</p><p>The plants for the treatment system were chosen to Typha latifolia and Scirpus species. The plants are growing in Tanzania and are being used for wastewater treatment purposes. The plants are tolerant to increased salinity and can grow in alkaline soil.</p><p>The cleaning effectiveness of the system was not tested, but only roughly estimated with help of values from a similar treatment system build in Gotland, Sweden.</p><p>Hence, the reuse of grey water in this geographical location suits best for irrigation in rural and peri-urban areas with potential for agriculture as well as groundwater recharge in the peri-urban and urban areas. Recharging groundwater with treated grey water has a potential to be observed as an indirect and long term reuse of the water as it reconnects in the hydrological cycle.</p>

The movement towards the provision of evidence-based services has become increasingly important to psychological practice (CPA Task Force on Evidence-Based Practice of Psychological Treatments, 2012; Levant, 2005). One of the key components of this movement is the summarizing and dissemination of important findings to key stakeholders for interventions using techniques such as meta-analysis. Meta-analyses are typically based on reviews of the published literature on a particular topic, but increasingly researchers are acknowledging the importance of examining and including unpublished, grey literature in their reviews in presenting an accurate picture of a body of literature (Hopewell, McDonald, Clarke, & Egger, 2008; McAuley, Pham, Tugwell, & Moher, 2000). However, the inclusion of grey literature in meta-analyses and reviews remains controversial (Coad, Hardicre, & Devitt, 2006; Martin, Perez, Sacristan, & Alvarez, 2005; Seymour, 2010). A number of reviews of behavioural parenting interventions have been conducted. The body of literature in this area continues to grow quickly, and recent meta-analyses in this area have examined only subsets of the literature, thus necessitating an overall examination of all available literature in this area: published and unpublished. The present meta-analysis synthesized the results of 42 published and 6 unpublished behavioural parenting intervention studies conducted between 1966 and 2011. In the first manuscript, treatment efficacy was examined with respect to three different outcomes (parent behaviour, child behaviour, and parent adjustment). I also examined the differential effects of six moderator variables in two categories: child characteristics (child formal diagnostic status and child age) and methodological characteristics (overall quality, comparison group, experimenter allegiance, and publication status). In the second manuscript, I examined both the advantages and challenges of including grey literature in meta-analyses, and explored the use of an innovative search program. I also explored the influence of the inclusion of grey literature on outcomes, including whether effect sizes, sample homogeneity, and publication bias indices are affected by the inclusion of grey literature. Results of the present study confirm the efficacy of behavioural parenting interventions on parent behaviour, child behaviour, and parent adjustment outcomes. Further, I extend the knowledge base through the inclusion of recent reports, grey literature, searches of basic search engines such as Google and Yahoo, and the examination of previously unexplored moderator variables. My results underline the importance of consistent reporting practices in the field and their impact on the availability of evidence for policy-makers, administrators, researchers, clinicians and consumers. I also discuss the implications of these findings for the provision of evidence-based services for parents, knowledge translation, and grey literature.

In den letzten Jahren ist zunehmend deutlich geworden, dass die Multiple Sklerose (MS) nicht nur eine Erkrankung der weißen Substanz des zentralen Nervensystems ist, sondern auch häufig und beträchtlich die graue Substanz in allen klinischen Verlaufsformen betrifft. Besonders die kortikale Pathologie mit entmarkten Läsionen wurde durch verbesserte immunhistochemische Färbetechniken und neuen magnetresonanztomographischen Verfahren ausführlicher untersucht. MS-Patienten leiden klinisch oft an körperlichen Beeinträchtigungen und neuropsychologischen Defiziten, welche die Lebensqualität beeinflussen. Diese Symptome wurden mit Läsionen in der grauen Substanz assoziiert. Mechanismen, die zu dieser Pathologie führen, müssen daher aufgeklärt werden um vorbeugende oder akute Behandlungen entwickeln zu können. Zur pathologischen Untersuchung der grauen Substanz werden angemessene Tiermodelle benötigt, welche die humane kortikale Pathologie wiederspiegeln. Das am häufigsten verwendete Tiermodell in MS-Studien ist die Experimentelle Autoimmune Enzephalomyelitis (EAE), die in ihrem ‘konventionellen’ Immunisierungsprotokoll nur selten den zerebralen Kortex betrifft. Ein EAE-Modell mit Einbezug des Kortex, das MS-Läsionen nachahmt, wurde in Ratten beschrieben. Hierzu wurden proinflammatorische Zytokine in eine vorbestimmte kortikale Region injiziert. Da spezifisch genveränderte Rattenstämme fehlen um die Mechanismen der Pathologie in der grauen Substanz zu untersuchen ist es notwendig das Tiermodell in Mäusen zu entwickelen. Das Ziel dieses Projekts war die Entwicklung eines kortikalen EAE-Mausmodells sowie dessen histopathologische Charakterisierung. Desweiteren wurde kortikales Gehirnmaterial von MS-Patienten im späten Krankheitsstadium auf dendritische Patholgie untersucht. Die kortikale EAE wurde in Myelin Oligodendrozyten Glykoprotein (MOG)-immunisierten BiozziABH (hohe Antikörper) und F1 Nachkommen, die aus BiozziABH und Mäusen mit einem C57BL6/J-Hintergrund generiert worden sind, durch die intrakortikale Injektion von TNF-α und IFN-γ induziert. Histologische Untersuchungen zeigten eine ausgedehnte subpiale Entmarkung und Entzündung im Kortex drei Tage nach der Zytokininjektion in der betroffenen Hirnhälfte. Die Entzündung ging innerhalb von drei Wochen fast vollständig zurück und entmarkte Regionen wiesen teilweise eine Remyelinisierung auf. Axone blieben in läsionalen Regionen erhalten und neuronaler Verlust wurde im Kortex nicht beobachtet. Desweiteren wurde eine Methode etabliert, die es erlaubt detailliert dendritische Pathologien in der Maus zu untersuchen. Kortex-enthaltenes Autopsiematerial von progressiven MS-Patienten mit langandauerndem Krankheitsverlauf zeigte einen Verlust von dendritischen Dornfortsätzen (Spines) in Neurone, die in den unteren korikalen Layern sowohl in chronisch entmarkten Läsionen als auch im umliegenden normal erscheinendem Gewebe der grauen Substanz lokalisiert waren. Im vorliegenden Projekt wurde ein kortikales EAE-Mausmodell entwickelt, das die humane MS-Pathologie der grauen Substanz in frühen Krankheitsstadien wiederspiegelt. Dieses Modell ist für Untersuchungen früher Mechanismen im entmarkten Kortex und für die Erprobung therapeutischer Behandlungen wie die Erhöhung der Remyelinisierung nützlich. Darüberhinaus wurde ein ausgedehnter Verlust dendritischer Dornfortsätze im zerebralen Kortex in chronischen MS-Patienten gezeigt, der auf oft beobachtete neuropsychologische Defizite zurückgeführt werden könnte.

Thesis (Ph.D.) - University of Queensland, 2003. / Includes bibliography.

No description available.

The red squirrel (Sciurus vulgaris) is native to Eurasia, but in the UK its survival is being threatened by the non-native grey squirrel (Sciurus carolinensis). Since its introduction to the UK from the USA the grey squirrel has increased its range at the expense of the red squirrel. Although competition for resources clearly plays a role in this replacement, an infectious viral disease, caused by squirrelpox virus (SQPV) and hosted apparently asymptomatically by the grey squirrels, has now been recognised as a major contributing factor. Little is known about the pathogenesis of infection in grey squirrels in comparison to red squirrels, but understanding this is essential to determining how the virus spreads within and between the red and grey squirrels. The aims of this thesis were to investigate the course of SQPV infection in red and grey squirrels and possible routes of virus transmission. Specifically, for the first time, a novel Real Time PCR (qPCR) assay and immunohistochemistry were used to investigate the presence of SQPV in various tissues from naturally infected red squirrels and experimentally infected grey squirrels. In diseased red squirrels SQPV DNA was found in several tissues with the highest amounts being found in skin samples. This reflects the multiple lesions that were easily visible on the red squirrel carcasses. There was no indication of systemic disease although the viral DNA was detected, at lower levels, in other internal organs. Grey squirrels were experimentally infected with SQPV isolated from naturally-infected red squirrels with fatal clinical disease. In contrast to SQPV-infected red squirrels no clinical lesions, other than mild scab formation at the site of inoculation, were found in the grey squirrels post-infection. No gross pathological changes indicative of systemic infection were observed and these findings were reflected in the qPCR and histopathology results. Viral DNA was only detected by qPCR in samples from the site of inoculation (scarified skin) and at lower concentrations in other skin tissues such as digital and eyelid skin. In addition, histopathology and immunohistochemistry examination revealed evidence of infection characterized by ballooning degeneration of keratinocytes, and acanthosis and spongiosis of the epidermis. These skin lesions were self limiting and minor compared to the infected red squirrel skin samples. The molecular variation in the virus isolated over time from different parts of the UK was also investigated. Seven SQPV isolates (4 from Scotland and 3 from England) were tested and results indicated that there are no significant changes in the amino acid sequence of any of the three genes examined apart from one amino acid change (one base change) in one gene. All Scottish isolates examined showed this change in comparison to English isolates. The results in this thesis show that there is a mild pathology associated with SQPV infection in grey squirrels. Scabs form at the site of infection but are less proliferative than in infected red squirrels, though they may still serve to contaminate the environment with virus leading to further outbreaks of disease. In contrast it seems likely that the proliferative lesions suffered by red squirrels and the greater amounts of virus that this leads to are likely to be more significant to the epidemiology of disease in localised outbreaks.

Recently, Magnetic Resonance Imaging (MRI) has found great traction in monitoring the effects of Caloric Restriction (CR) on the brain, specifically gray matter. However, there are no streamlined, simple pipelines in existence to analyze data generated from these kinds of MRI studies. Therefore, my hypothesis is two-fold: the first part being the development of a dynamic and straightforward image processing pipeline, which I have tailored to fit the unique needs of the CR data involved in this study. This data brings me to the second part of my hypothesis, which is to use that pipeline to highlight the decreased attenuation in grey matter induced by long-term CR. In order to test the second portion of my hypothesis, T1/MPRAGE scans were collected from 17 male Rhesus Macaques, half of which were maintained on a 30% reduced calorie diet for an average of 22 years, starting around age 3. Using this basis, the inherent properties of the MR images were exploited by the novel pipeline, and used to analyze whether or not CR reduces the attenuation of grey matter atrophy, with regards to aging

Animal communication systems are complex, but in many species relatively little is known about how they develop. Insight into the development of adult repertoires, considering how factors such as genetics, environment and learning contribute to signal changes, provides a more comprehensive understanding of communication. This thesis documented vocal learning and development in the grey seal. In chapter 3, vocal repertoires of grey seal pups were recorded from birth through their first year, and were compared to calls of other seals across life stages, from pups to adults. By examining call parameters that were similar in animals of the same age, size, and sex, changes that may be attributed to physical development and morphology were identified. Past studies have indicated that seals may also have advanced vocal learning abilities, however the extent of these capabilities was unknown. In this thesis, vocal learning in juvenile grey seals was tested considering both how the seals learn to produce calls as well as gain information from perceived sounds. In chapter 4, grey seals were shown to be capable of vocal usage learning by producing specific calls in specific contexts. In chapter 5, grey seals displayed control over the structure of their calls as they could imitate novel sound models, demonstrating vocal production learning. Lastly, in chapter 6 grey seals gained information from perceived sound signals and used them to their benefit to locate food, demonstrating acoustic comprehension learning. Adult grey seals have complex call repertoires, and in this thesis I have shown how their calls develop with physiological growth and experience, as well as how they can gain information from perceived sound signals.