This study used DHI records of 1,023,827 Holstein cows to determine the impact of adjusting measures of profit for opportunity cost and for evaluating the impact of herd-year variables and registry status on measures of profit. Relative Net Income (RNIJ) and RNI including opportunity cost (RNIOC), the revenue forfeited that could have been generated by a replacement heifer, were calculated for 48 month, 60 month, 72 month, and all lactations herdlife period. Application of opportunity cost caused a decreased value for length of herdlife. Number of lactations initiated prior to the various herdlife periods was the criteria for deciding how much information was included in each of the herdlife periods. Opportunity cost per day (OCPD), the revenue forfeited that could be earned by replacement, was calculated by regressing herd-year means for RNI and days of productive life (DPL) on overall herd means based on the number of animals in herd-years, and dividing the regressed mean for RNI by the regressed mean for DPL. RNIOC for each herdlife period (i) was calculated as RNI(i) - (OCPD(i) * DPL(i)) for each cow in a herd-year. Application of OCPD removed all of the variation due to herd and year for RNIOC. Standard deviations and means were smaller for RNIOC than for RNI. This was because there were fewer animals with extremely large values for RNIOC than for RNI. These differences increased as herdlife period advanced. The correlation of RNI and RNIOC to 72 mo herdlife was .95. This was caused by a lower correlation with DPL for RNIOC than RNI (.73 vs. .84). First lactation yield had similar correlations with both measures. Regression analysis showed all of the year within herd variance for profit measures and no error variance could be explained by herd management variables, with average first lactation milk dominating. Grade cows were lower than registered cows for profit measures within herd, and differences increased as herdlife period advanced. / Master of Science

The ability of mammary tissue from mature Holstein bulls to respond to hormonal stimuli in organ culture to synthesize and secrete milk proteins was studied. Sixteen bulls from the genetic selection and control lines developed at Virginia Polyteclmic Institute were randomly assigned to either a non-treated or steroid pretreated group. The steroid pretreated group received estradiol 17-B and progesterone at 0.1 and 0.25 mg/kg/day, respectively, for 7 d starting 15 d before slaughter. Mammary tissue was explanted and cultured for up to 96 h in basal medium (B) which consisted of Media 199 supplemented with fetal calf serum and bovine insulin, or stimulatory medium (St), which was further supplemented with triiodothyronine, estradiol 17-B, hydrocortisone, and bovine prolactin (PRL). Explants in duplicate multi-well culture dishes were incubated in the presence of 3H-Iabeled amino acids or 3H-Iabeled thymidine. Measurements made included: casein in media and homogenized tissue, alpha-lactalbumin in homogenized tissue, tri-chloro-acetic acid precipitable 3H-protein in media and homogenized tissue, and DNA content of cultured tissue. A subset of cultured explants from each bull was fixed and embedded for histological evaluation and auto radiographic localization of incorporated 3H-labeled thymidine. Synthesis and secretion data, as well as growth responses of the epithelium were compared between selection and control bulls. / Master of Science

Two experiments with dairy cows during early lactation were conducted to determine the effects of feeding feather meal and fat. The objective of Experiment 1 was to determine milk production, milk composition, feed intake, and concentrations of AA and long chain fatty acids in blood plasma. Thirty two Holstein cows began a six week trial at the start of the fourth week of lactation. Diets included a control diet (51% forage, 49% concentrate, 16.8% CP, and 19.1% ADF) and diets containing 2.2% feather meal, 3.6% supplemental fat (hydrogenated tallow), or feather meal plus fat. Feather meal and supplemental fat were substituted for soybean meal and corn grain, respectively, in the control diet concentrate. For Experiment 2, four lactating Holstein cows (15, 20, 21, and 65 d postpartum) with ruminal and duodenal cannulae were used in a 4X4 Latin square design (21 d feeding periods) to determine digestibilities of the four diets. The markers used to estimate digesta flow were Co- EDTA and chromic oxide. When cows were fed feather meal, milk fat % was higher, milk protein % was lower, and plasma total essential AA were increased. Dry matter intake, milk production, and plasma long chain fatty acids increased when cows were fed diets containing fat. Differences between concentrations of AA in the tail artery and mammary vein indicated significant uptake by the mammary gland. The mammary gland extracted approximately 36% of the total essential AA in plasma. Intake, duodenal flow, and ruminal and total tract digestibilities of DM, ADF, N, and OM were similar for all diets. No changes were seen in rumen pH, rumen ammonia-N, VFA's, or rumen bacteria AA. Intake and fecal output of total, essential, and non-essential AA were greater when cows were fed diets containing feather meal. However, flow of AA to the duodenum, AA absorbed, and AA digestibilities did not differ. Results suggest that bypass proteins with a complimentary EAA profile must be fed with feather meal to compensate for the low amounts of Lys, Met, and His provided by feather meal. / Master of Science

Complete data is given of a digestion trial made with two Holstein cows, one being feed maintenance ration plus protein, the other maintenance ration plus carbohydrate. The digestive co-efficient of both cows were about normal. The cow which received the maintenance ration plus B. Albumen digested 67.87% of whole ration and the one which received maintenance ration plus carbohydrate digested 66.10% of whole ration. Both of which is a little lower than the theoretical. The following authorities have been consulted: Echol’s Missouri Station Bulletin Research 2 and 5. Henry’s, Shermans, Armsby’s, and Farmers Bulletin 346. Bulletin 22 Revised Department of Agriculture. / Master of Science

De Kol 2d, Pietertje Hengerveld and Belle Koendyke each have two daughters. For each daughter the following number of grand daughters are found. For Belle Korndyke 93.5, De Kol 2d 81.5 , and Pietertje Hengerveld 60.5. The following number of great-grand-daughters may be attributed to each grand-daughter: De Kol 2d 5.29, Belle Korndyke 3.11 and Pietertje Hengerveld 2.96. This shows De Kol 2d was more prepotent over her great-grand-daughters or her daughters were bred to better sires. The percent of the progeny found in each class clearly shows the prepotency of the progenitor. The most noticeable influence over the grand daughters of the first class was that of Belle Korndyke, having 16.52% of progeny in first class. De Kol ranked second and Pietertje Hengerveld third. In class two Belle Korndyke ranks first, De Kol 2d second, and Pietertje Hengerveld third. In classes three and four some change is order is noticeable; Pietertje Hengerveld ranks first, De Kol 2d second, and Belle Korndyke third. / Master of Science

The two objectives for this thesis were to 1) validate a bovine luteinizing hormone (LH) ELISA for use in the laboratory and 2) validate interstitial glucose sensors designed for humans for use in lactating dairy cattle. The first experiment required validation of a bovine LH ELISA in order to measure the circulating concentration of LH in the blood of cows in altered thermal and/or metabolic states. Assays from two separate companies were tested. Half of one plate was run at a time resulting in a total of 6 separate analyses (3 plates total). Despite early, promising results, neither LH ELISA could be successfully validated. For many analyses, the standard curves did not even meet the minimal criteria to allow calculation of a formula for determining the concentrations of the unknowns. In analyses where the standard curves were acceptable, the coefficients of variation (CV%) for the unknowns were unacceptable. The second experiment attempted to validate the use of commercially available human interstitial glucose sensors (FreeStyle Libre and Dexcom G6) in lactating dairy cows. Blood glucose concentrations correlated well with sensors secured behind the cow's ear for both FreeStyle Libre (r=0.82) and Dexcom (r=0.88). Unfortunately, however, the absolute relative error's highest value was 47% for the FreeStyle Libre ear. In summary, neither the LH ELISAs nor the interstitial glucose sensors could be validated. / Master of Science / Two separate studies were performed with the purpose of one, measuring a hormone significant to reproduction, Luteinizing Hormone (LH), and two, measuring glucose in interstitial fluid in lactating cows. The first experiment required the use of a commercially available ELISA test to measure LH concentrations. While using radioimmunoassay (RIA) is the preferred method for measuring LH in dairy cattle, an ELISA is an alternative method that is generally more accessible. There was also little published research available illustrating the accuracy of the LH ELISAs. Therefore, we attempted to validate the assays for bovine samples. Despite several attempts, the LH ELISA was unsuccessfully validated. The second study attempted to validate commercially available continuous interstitial glucose sensors designed for humans for use with lactating Holstein cows. Validation of interstitial sensors for use in dairy cattle would decrease the amount of stress and handling when measuring circulating glucose for research, clinical diagnostics and application in industry. While early data suggested that sensors behind a cow's ear may work, further analysis illustrated the sensors were not accurate enough when used on cows. In conclusion, the lab was unsuccessful in proving that continuous interstitial glucose monitors and LH ELISAs could be utilized in the lab.

Dairy cattle characteristically exhibit decreased milk yield and reproductive performance, as well as increased uterine infection rates during periods of high stress. Chromium supplementation has demonstrated efficacy as a feed additive capable of reducing the detrimental effects of stress. As such, its application in dairy production may help to alleviate economic losses associated with seasonal heat stress and the stress experienced during the early postpartum period. Therefore, the objective of the work described in this thesis was to evaluate the potential benefits of short-duration, high-dose chromium (Cr) supplementation in early postpartum dairy cows during the summer months. Multiparous, early postpartum cows (20.95 ± 0.21 DIM, 658.29 ± 13.61 kg) were assigned to one of two treatment groups: 1) normal TMR (Con; n=10) and 2) normal TMR + Cr propionate supplementation (CrPro; 12 mg/h/d Cr; n=12). Body weight (BW), milk yield, and feed intake were measured each day of the experiment. Ambient temperature and humidity were monitored, and the temperature-humidity index (THI) was calculated for the duration of the study as an indicator of the severity of the heat stress experienced by the cows. Transrectal ultrasonography was performed every three days to assess ovarian follicular and luteal dynamics. Respiration rates (RR), rectal temperatures (RT), and blood glucose were recorded concurrently with ovarian ultrasonography. Plasma was collected and used for analysis of progesterone concentrations. Every six days in conjunction with ultrasonography, endometrial cytology samples were collected via cytobrush from each cow to determine the incidences of subclinical endometritis (SCE), as determined by polymorphonuclear leukocyte (PMNL) %. There were no treatment-based differences in RR, RT, blood glucose, feed intake, milk yield, or BW. However, the supplementation strategy did improve reproductive parameters. Within the Con group, there was an increase in PMNL % between samples five and six. Furthermore, at cytology sample six, the Con group had a greater percentage of PMNL than the CrPro group (P=0.01). Chromium consumption did not affect the counts or sizes of most follicles, with the exception being the 6-9 mm category where the CrPro group had a greater average diameter and tended to have more follicles in this category. While CL numbers or size did not differ between treatments, the ratio of progesterone (P4) to corpus luteum (CL) volume was greater in the CrPro group compared to the Con group (P=0.03). The results from this study indicate that the proposed supplementation strategy does not influence DMI or milk yield in cows experiencing stress. Nonetheless, short-duration, high-dose Cr supplementation strategy could benefit reproductive performance and thereby limit economic losses experienced by dairy producers during periods of stress. / Master of Science / Dairy producers continue to experience monetary losses due to the decrease in production performance by dairy cows resulting from physiological changes in response to stress. Elevated ambient temperature and humidity conditions can lead to heat stress, which has been found to decrease both milk and reproductive performance on dairy operations. The period after calving, known as the postpartum period, can also lead to metabolic changes in a cow due to the stress of giving birth and beginning to produce milk. Chromium is an essential trace mineral that can be supplemented with cattle feed to improve the impacts of such stressful periods on production parameters. This study evaluated the effect of a short-duration, high-dose chromium supplementation strategy on reproduction and lactation of early postpartum dairy cattle during summer months. Twenty-two cows were assigned to two treatment groups: control (standard feed only) and chromium supplement (standard feed plus chromium propionate supplementation). Cows receiving the chromium supplement did not experience any changes in respiration rate, rectal temperature, blood glucose levels, feed intake, milk yield, or body weight. Reproductive analyses determined that cows receiving the chromium supplementation had a lower number of immune cells present in the uterus by the end of the experiment, which may be indicative of a healthier uterine environment. Chromium supplemented cows also had a greater level of progesterone concentration to corpus luteum volume compared to cows in the control group. These results indicate that the short-duration, high-dose chromium supplementation strategy could benefit reproductive performance and limit losses experienced by dairy cows under stress conditions.

Data sets from six locations containing 631 dairy heifers and 5409 observations of individual dry matter intake (DMI) were used to develop an equation to predict DMI. Data were contributed by Pennsylvania State University, University of New Hampshire, Purina Mills, Kansas State University, University of Minnesota, and Virginia Tech. Objectives varied by location and trials within locations but all contained DMI (kg/d), BW (kg), age (wk), DM, and CP, and ADF percentages of ration DM. Daily gains (g) were calculated from consecutive BW. Season was expressed as sine wave of Julian day plus 60 d times Ï divided into 180. Average daily temperature, humidity, and wind data were collected from Minnesota and Virginia and analyzed with BW to determine their effect on DMI. Sire PTA milk, protein, and fat of heifers were recorded from all sources to determine the relationship of genetics with DMI. Diet measurements (DM, CP, and ADF percent of ration DM) were analyzed with BW to determine their impact on DMI. Two equations were developed using backwards elimination techniques. The first equation was: DMI = -12.63 + .0587(BW) - .0000264(BW2) - .000 12(BWxDM) - .000477(BWxADF) + .292(DM) - .00103(DM2) - .413(CP) + .01349(CP2) + .181(ADF) - .0025(DMxCP) - .00269(DMxADF) + .00509(CPxADF) with an R2 of .90. Because body weight and diet variables were highly correlated, a smaller model could be created with one diet variable. A smaller model would also be more practical to use if accuracy was not lost. Body weight and ADF were used for second model: DMI = -1.71 + .0429(BW) - .0000246(BW2) - .00023(BWxADF) + .032(ADF) - .00068(ADF2) with an R 2 of .87. Previous DMI prediction equations from Virginia Tech were validated using all data from other locations and had R2 of .90 and .84 with this data set. Body weight raised to the .53 power most accurately described the relationship of DMI and BW. Temperature and DMI had a quadratic relationship. Higher DMI were observed at extreme temperatures between -10 to 27° C. Humidity and temperature x humidity accounted for more variation of OMI than season and other environmental measures, but were not included in the small model due to availability of these measurements and they did not change OMI by .1 kg/SO. Sire PTA milk by groups showed differences among heifer groups although no trends were found. Dry matter percent and CP percent had linear and quadratic relationships with OMI. Maximum OMI plateaued between 75 and 950/0 OM and occurred at extreme percentages for CP between 9 and 27%. Acid detergent fiber percent had a negative linear relationship with OMI between 7 and 45%. / Master of Science

En début de lactation, la vache subit un stress important occasionné par l’impossibilité de combler l’ensemble de ses besoins énergétiques par sa consommation exogène. Cette période spécifique se caractérise par une balance énergétique négative, entraîne une utilisation excessive des réserves corporelles de l’animal et représente un défi métabolique important. Ironiquement, depuis maintenant plus de 40 ans, le système incite les producteurs laitiers à effectuer l’insémination au jour 60 post-partum, c’est-à-dire au moment où la vache rencontre un déficit métabolique. Ce déficit au moment de la conception aurait un impact chez la progéniture, notamment au niveau épigénétique. Ce projet consiste à documenter l’effet de la balance énergétique négative sur la qualité de l’embryon et, en l’occurrence, à proposer des pistes afin d’améliorer la fertilité des bovins laitiers. La mesure du bêta-hydroxybutyrate (BHB) a été effectuée à partir d’échantillons sanguins entre 45 et 60 jours post-partum sur dix-huit vaches de race Holstein. Selon la mesure obtenue, la vache fut classée comme étant faible ou élevée en BHB, afin d’avoir au moins six vaches par groupe. Après un processus de stimulation ovarien, chaque vache fut inséminée et les embryons, récoltés. Pour chaque vache, deux embryons ont été transférés dans deux primipares, afin de déterminer subséquemment la persistance des marqueurs dans le matériel biologique. Grâce à la plate-forme EmbryoGENE, il fut possible de déterminer l’expression génique ainsi que l’état de méthylation de l’ADN des embryons récoltés. Les résultats obtenus soutiennent l’existence d’une altération du métabolisme énergétique au niveau embryonnaire, notamment par la modification de la voie de signalisation de mTOR ainsi que celle des sirtuines. Cette altération semble se traduire par une dysfonction mitochondriale et une inhibition de la transcription, entraînant un freinage au niveau cellulaire, probablement dû à la programmation de l’embryon à utiliser ses réserves lipidiques lors de conditions importantes de stress. / In early lactation, the cow undergoes an important stress generated by the impossibility of filling its entire energetic needs by exogenous consumption. This is characterized by a negative energy balance, excessive use of animal body reserves and represents an important metabolic challenge. Ironically, for more than 40 years now, the system has been encouraging dairy farmers to inseminate on day 60 postpartum, when the cow has a metabolic deficit. This deficit at the time of conception could impact the offspring, especially at the epigenetic level. This project is meant to document the effect of the negative energy balance on the quality of the embryo and to identify ways to improve the fertility of dairy cows. The beta hydroxybutyrate (BHB) measure was done from blood samples between day 45 and 60 postpartum on eighteen Holstein cows. According to the measure obtained, each cow was classified as low or high in BHB, so as to have at least six cows per group. After an ovarian stimulation process, each cow was inseminated and the embryos were harvested. For each cow, two embryos were transferred in two primiparous cows in order to subsequently determine the persistence of the markers in the biological material. With the EmbryoGENE platform, it was possible to determine the gene expression as well as the methylation status of DNA embryos. The results obtained support the existence of an alteration of the energetic metabolism at the embryonic level, especially by the modification of the signaling pathway of mTOR as well as those of the sirtuins. This alteration appears to result in mitochondrial dysfunction and inhibition of transcription, leading to a reduced activity at a cellular level, probably due to programming of the embryo to use its lipid reserves during severe stress conditions.

A total of 114,351 Holstein first lactation records collected by the Quebec Dairy Herd Analysis Service (DHAS-PATLQ) between September, 1979 and January, 1994 were used to study the effects of adjusting records for linear and quadratic effects of 90- and 305-d feed intake measures (total energy, total protein and total dry matter) on estimation of heritabilities of and genetic correlations among yield and composition traits. Genetic parameters of feed intake measures, relationships between feed intake and age and weight at calving, and feed conversion efficiency traits were also examined in a series of studies. A sire model fitted by REML estimated heritabilities of 305-d milk, fat and protein yields, fat and protein percents as .45 +/- .04, .48 +/- .04, .44 +/- .03, .92 +/- .06, and .88 +/- .05, respectively. Correction of 305-d records for differences among cows in feed intake levels reduced heritabilities of milk, fat and protein yields to .35 +/- .03, .52 +/- .04, and .38 +/- .03, respectively. Heritabilities of composition traits (fat and protein percents) remained unchanged. Genetic and phenotypic correlations for yield traits were also reduced (48--170%, and 16--51%, respectively) which may indicate that genetic associations between yield traits are less than what we believed them to be. Heritabilities of 305-d total energy, total protein, total DM intake, grain energy, grain protein, grain DM, base energy, base protein, and base DM were .30 +/- .03, .24 +/- .02, .35 +/- .03, .23 +/- .02, .23 +/- .02, .23 +/- .02, .31 +/- .03, .26 +/- .02, and .40 +/- .03, respectively. Genetic correlations between feed intake measures were very high; they were approaching unity in some cases. Therefore, any one of these feed intake measures can represent the others. A multi-trait REML analysis estimated heritabilities of age and weight at first calving as .11 +/- .01 and .37 +/- .03, respectively. Age at calving was negatively associated with yield traits and with total e