The size of the apiculture industry in Western Australia (W.A.) is one of the smallest in the nation but the production of pollen and honey per colony is the highest in Australia. The overwhelming value of the bee industry to the community is through pollination. The pollination service benefit provided by honey bees (Apis mellifera) in Australia has an estimated value of $AUS1.7 billion (1999 - 2000). The economic yields from crops, such as almonds and cucurbits, depend entirely on the activity of honey bees. Access to flora is essential to maintain productive colonies for pollination services. Pollen and nectar from flowers provide the nutritional components for colonies of honey bees to breed, but pollen is more important as it provides the colony with its source of protein. Protein content is changed by pollen lipid content which can vary from 0.8 to 18.9 %. Lipids are composed of fatty acids and a number are highly antimicrobial and play an important role in colony hygiene, whilst others are nutritionally crucial for honey bee development. Australian honey bee colonies utilise areas of native flora where a diversity of pollen species exist or hives are placed with agricultural crops that are based on European plants grown in monocultures, e.g. canola. Anecdotal evidence suggests that, in terms of breeding bees, some pollen species are much better than others and that bee health and longevity can be compromised if pollen is derived from single plant species. Protein analysis of pollen has been conducted on a wide range of species over the last two decades. However, lipid content and its analysis for fatty acids, which was reviewed for this thesis, have only been conducted on a few species. An initial investigation into the fatty acid composition of the pollen of W.A. eucalypts revealed the genus was characteristically high in linoleic acid concentration and ranged from 35.7 – 48 % (2.77 – 5.81 mg/g). Of the six species that are important to W.A. beekeepers, Eucalyptus wandoo (whitegum) and E. accedens (Powderbark Wandoo), a taxonomically similar species, showed similar levels of arachidic acid, whilst all three E. wandoo flowering varieties (summer, winter and spring) were the lowest in linolenic acid. Corymbia calophylla (redgum) was significantly higher in myristic and linolenic acids and E. patens (blackbutt), E. marginata (jarrah) and E. diversicolor (karri) had similar fatty acid profiles. European honey bees have evolved with plant species that have pollen that contain much higher levels of lipids, which are dominated by linolenic acid, than eucalypts. By contrast, the pollen of eucalypts, the most targeted plants by W.A. beekeepers, and other Australian plants are typically higher in linoleic than linolenic acid. Given the influence of lipids on protein content and that fatty acid concentration varied amongst some of the important eucalypt species, a much wider study of pollen from plant species that are important to beekeepers was conducted. The first aim of the project, a national pollen survey, was undertaken in which 577 samples of pollen were collected. A total of 73 different fatty acids were identified. Of these, only five: palmitic, stearic, oleic, linoleic and linolenic were common to all 577 samples of pollen. The second aim of the thesis was to investigate the effect of two commonly found fatty acids in pollen and their concentration at which longevity and life-span of honey bees, and development of the hypopharyngeal gland were deleteriously affected. An associated objective was to determine whether a range of soya bean flours, the main ingredient of an artificial bee diet that can replace pollen but differs in lipid concentration, had a similar effect. The aim encompassed two projects. In the first, two fatty acids (oleic and linoleic acid) were added at concentrations from 0 to 16 % to the low-fat, bee-collected pollen from C. calophylla. Eight different lipid-enhanced diets were created and each fed to bees confined in cages (mini-colonies containing 1400 bees). Oleic and linoleic acids were chosen because they are two of the five commonly found fatty acids in pollen. Oleic acid is the dominant fatty acid in honey bees and is a monounsaturated fatty acid. Linoleic acid is a dominant fatty acid in eucalypt pollen and is a polyunsaturated fatty acid that is one of two essential fatty acids that has antimicrobial activity. The second project revolved around the problem of maintaining bee populations when apiaries are in environments that lack floral abundance due to drought or other environmental catastrophes. In these situations, beekeepers maintain their colonies by supplying artificial feedstuffs to colonies of bees. The high-protein diet ingredient of choice is imported soya bean flour and three flours containing 0.6 % (protein concentrate), 1.8 % (defatted) and 18.9 % (full-fat) lipid, were used. Locally milled lupin flour, containing 6.9 % lipid, was tested as a possible replacement for imported soya bean flour. As for soya bean flour, lupin flour was used in pure form or mixed with pollen in diets fed to bees. Flour and pollen combinations created another ten different diets fed to bees the same way as the fatty acid-enhanced pollen diets. Along with the 8 lipid-enhanced and 10 flour and flour-pollen diets, there were two sugar-only diets, one mixed from dry cane sugar and the other, a liquid invert sugar. Two redgum pollen-only diets concluded the suite of 22 diets tested. One of these pollen diets was crushed and irradiated and was several years old whilst the other was collected fresh at the beginning of experimentation and kept frozen. Crushed and irradiated pollen is in common use by Australian commercial beekeepers as feedback when conditions for floral abundance are adverse. An early experimental result was an observation of distinctive bee behaviour after bees were confined in cages for six weeks where small but persistent numbers of bees were found hairless in samples. The behaviour was apparently the same as when single cohorts of emerged bees rearrange their caste repertoire, which has been reported elsewhere, but where no connection to head weight and caste type had been documented. Low head weight and hairlessness were strongly associated with each other. Low head weights are usually associated with foraging honey bees because the hypopharyngeal gland is no longer developed functionally. Experimentally, bees were assessed for longevity to 22 different diets in 7 experiments. Laboratory analysis was conducted on the weekly samples of bees removed from cages where bees were measured for head weight (hypopharyngeal gland development) and nutritional status by analysing de-gutted bees for protein, lipid, mineral and fatty acid content. Of the 22 diets tested, pure redgum pollen diets gave the greatest life-span and those bees fed diets of pure sugar had the shortest life. Honey bees fed a low-fat protein concentrate from soya bean flour had the longest life of the flours tested. Adding pollen to soya bean flour diets improved longevity whereas the addition of pollen to lupin flour caused increased mortality. Defatted and full-fat soya bean flours gave similar longevities and, despite large differences in fat content, the response to diet of head weight was negligible to the diets and no response was elicited by the queen bee to lay eggs which also indicated failed gland development of the worker bees. The addition of fatty acid (oleic and linoleic) to pollen at different concentrations caused significant differences in longevity. Overall, the addition of both fatty acids to pollen did not improve longevity. The addition of oleic acid to pollen greater than 2 % caused the longevity of bees to decrease, a poor head weight response and a failure of the queen to lay eggs. The addition of linoleic acid greater than 6 % to pollen diets had a similar response. As the percentage of oil was increased for both fatty acid additions, total consumption of the diet decreased. Honey bees fed soya bean, lupin flour and sugar-only diets failed to accumulate linoleic acid in their body which was in contrast to honey bees fed pollen diets. For the sugar diet, the failure of linoleic acid accumulation in bees occurred despite bees being able to accumulate total lipid. Manganese was poorly accumulated by honey bees fed both soya bean and lupin flour diets and a sugar-only diet. The implication is that linoleic acid and manganese need to be added separately to dietary formulations in a form as yet to be determined that will enable honey bees to accumulate these elements in the same way as bees do from consuming pollen. Soya bean flour-based diets, which have been used by beekeepers for decades, or lupin flour require additional amounts of linoleic acid and manganese. Similarly, this might apply to sugar. Sugar can be fed to bees in great quantities to enable bees to successfully over-winter in cold climates or it can allow breeding to commence which subsequently stimulates the collection of pollen. Sugar-feeding is widely promoted for orchard pollination, especially for kiwifruit. These changes could make these dietary ingredients more effective in enabling bees to breed between nectar flows and be more productive or nutritionally healthier, but any changes would require further cage experimentation. Bee-collected pollen naturally high in concentrations of oleic acid should also be tested in longevity trials, in conjunction with pollen that is low in oleic acid. The three untested common fatty acids (stearic, palmitic and linolenic acid) should also be evaluated for honey bee longevity and nutritional status.

Thesis (Ph. D.)--Columbia University, 1925. / Vita. Bibliography: p. [46-47].

Thesis (B.S.)--University of Wisconsin, 1917. / "A thesis submitted for the degree of Bachelor of Science in Agriculture." eContent provider-neutral record in process. Description based on print version record.

Thesis (M. Phil.)--University of Hong Kong, 1998. / Includes bibliographical references (leaf 161-176).

Thesis (M.S.)--University of Wisconsin--Madison, 1942. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 27-34).

Thesis--Berlin. / Biography, p. [71].

Resumo: O objetivo deste trabalho foi caracterizar a qualidade higiênico-sanitária do mel produzido na região do Pólo Cuesta do Estado de São Paulo, por meio da enumeração do Número Mais Provável de Coliformes Totais e Termotolerantes, contagem de bolores e leveduras e atividade de água. Foram coletadas 37 amostras de méis de diferentes floradas produzidas por abelhas Apis mellifera L. em apiários distribuídos na região. As amostras de mel foram coletadas no momento do beneficiamento, acondicionadas em embalagens plásticas do apiário e armazenadas à temperatura ambiente. Todas as análises foram realizadas segundo as recomendações do Ministério da Agricultura, Pecuária e Abastecimento no Laboratório de Microbiologia de Alimentos do Instituto de Biociências da UNESP de Botucatu. Todas as amostras revelaram-se negativas quanto à presença de coliformes totais e termotolerantes e 34 (91,9%) apresentaram contaminação por bolores e leveduras, variando entre 102 até 105 UFC/g. Quanto a atividade de água, os menores valores foram observados na florada de laranja com média de 0,575±0,01 Aa, diferindo significativamente das floradas de eucalipto (0,629±0,02 Aa) e silvestre (0,614±0,02 Aa). Conclui-se que a qualidade microbiológica do mel produzido na região do Pólo Cuesta do Estado de São Paulo apresenta-se satisfatória / Abstract: The objective of this study was to characterize the hygienic quality of the honey produced in the Pólo Cuesta region of São Paulo state by enumerating the Most Probable Number of Total Coliforms and Thermotolerants, by counting yeasts and molds, and by water activity. Thirty-seven samples of honey of flowerings produced by Apis mellifera L. bees in apiaries spread throughout the region were collected. The 28 honey samples were collected at processing, kept in plastic packages of the apiary, and stored in room temperature. All the analyses were carried out according to the recommendations of the Ministry of Agriculture, Livestock and Supply in the Laboratory of Food Microbiology of the Bioscience Institute at UNESP - Botucatu. All the samples proved negative as to the presence of total coliforms and thermotolerants and 34 (91.9%) of them presented some contamination by yeasts and molds ranging from 102 to 105 UFC/g. As to water activity, the lowest values were observed in the orange flowering with an average of 0.575±0.01 Aa, which is significantly different from the eucalyptus flowering (0.629±0.02 Aa) and from the sylvestral one (0.614±0.02 Aa). It is concluded that the microbiological quality of the honey produced in the Pólo Cuesta region of São Paulo state proves satisfactory / Orientador: Ricardo de Oliveira Orsi / Coorientador: Vera Lúcia Mores Rall / Banca: Ary Fernandes Júnior / Banca: Juliana Célia Denadai / Banca: Margarida Maria de Barros / Banca: Lidia Maria Ruv Carelli Barreto / Doutor

Populations of honey bees have declined worldwide in recent years. One suspected cause is the widespread use of pesticides in agriculture. Experiments were conducted to examine potential exposure routes of pesticides to honey bees in the Midsouth. Neonicotinoid seed treatment compounds were studied to determine the rate at which they drift during planting and the rate at which they diminish in crop tissue during crop development. Honey bee foraging activity in Midsouth crops was observed to determine when and at what densities foraging honey bees could be active during pesticide applications. This project was designed to aid in understanding the risks that pesticides could potentially pose to honey bees in the Midsouth.

No description available.

No description available.