The aim of this thesis is to investigate the environmental changes across the Mesolithic-Neolithic transition (c.7000-5000 cal BP) at two sites in north-west Europe. Specific research questions focus on the role of fire, the interaction of climate and environmental change and human impacts, and the degree of continuity across the transition. Previous work has led to hypotheses of human impacts in the late Mesolithic, usually through the use of fire, increasing the abundance of food. Detection of these practices and the change to farming in the Neolithic has long been the study of pollen analysts, but in this project additional techniques of NPPs, size-class differentiated charcoal, and silicon and titanium were added at high resolution in order to determine the relationships between the different forcing factors on mid-Holocene environments. Sites were selected close to locations where known later Mesolithic artefacts have been found, with dated archaeological excavations. An upland UK bog site (Dan Clough Moss, near March Hill, West Yorkshire) and a lowland Swedish lake (Bökeberg, Skåne) provided contrasting environments, and enabled a range of proxies to be used from terrestrial peat and limnic sediments. 14C dates from selected macrofossils enabled an age-depth curve to be produced from each profile, with a Bayesian model applied to estimate the age of each sample. Results show a detailed record of woodland change from both areas. At Dan Clough Moss, disturbance phases with evidence of local fires occur frequently (typically every 20-30 years) in the late Mesolithic, and have low magnitude but consistent records of coprophilous fungi. Some phases of disturbance are different however, without the fungal spore evidence, and with heath plants increasing in representation. Drier phases appear to correlate with more local fire, and increased hazel. The transition is marked by a change to longer duration but distant fires, and longer periods of woodland disturbance, increased ruderal species and more heathland. The dates of occupation phases show a late survival of Mesolithic practices, overlapping with the Neolithic by around 300 years. At Bökeberg, a contrasting pattern is shown, with longer-duration phases of inferred human impact being replaced by shorter episodes of fire-associated disturbance after the date of the transition. Pollen and spore zones of disturbance concur with the dated occupation of late Mesolithic sites at the former lake edge. There is some evidence for markedly wetter, and then significantly drier, climate through the transition, and it could be inferred that this influenced the change in food production economies. However, the overall landscape changed only subtly, with more evidence of potential weeds of cultivation. At Bökeberg, there was no overlap- both radiocarbon and palynology suggest an abrupt transition from Mesolithic to Neolithic. The landscape impact of the transition from Mesolithic to Neolithic at both sites was not a clear and consistent one. While Ulmus decline levels and thereafter had increases in weed species and other herbs the overall balance of trees and shrubs changed less than 20%. At both sites, climate may have been influential, although the evidence is inconclusive. Fires were important at both sites and in both periods, but at different scales and duration. Disturbance phases varied within the Mesolithic as well as between the Mesolithic and the Neolithic.

Tropical freshwater ecosystems are understudied and not well understood relative to temperate systems; however, they are becoming increasingly imperiled by escalating anthropogenic impacts. The aim of this thesis was to investigate how tropical freshwater macroinvertebrate communities changed both structurally and functionally over a pristine-rural-urban land-use gradient, in relation to different spatial and temporal scales, as well as to the availability of potential food sources. Fifteen streams in the Panama Canal Watershed were sampled during the dry and wet seasons of 2007 and 2008, for macroinvertebrate communities (benthic and leaf litter), environmental variables, and potential food sources. Along the land-use gradient, in both habitat types, taxon richness, diversity, and evenness all decreased significantly; whereas, abundance increased significantly. For the benthic macroinvertebrate community, unique variation was explained equally well by local (water chemistry and sediment type) and landscape (riparian vegetation and watershed land use) characteristics in the dry season, and landscape characteristics explained slightly more variation in the wet season. Leaf-litter macroinvertebrate community unique variation was better explained by local variables than by landscape variables in both seasons. In terms of potential food resources, fine detritus and inorganic material were the most common across all streams (increased quantities in urban streams) and seasons; whereas, the availability of diatoms and leaf material increased in the dry season. Using gut content analyses, we found that collectors (gatherers and filterers) were by far the most common functional feeding group, increasing in abundance along the land-use gradient. Predators, shredders, and scrapers were all most abundant in pristine streams and decreased along the land-use gradient. Finally, using seven community metrices, a potential biomonitoring tool was developed, the Neotropical Low-land Stream Multimetric Index (NLSMI), which distinguished well among the different levels of stream impairment. This study demonstrated that tropical communities were negatively affected by human land alteration, but that community responses depended on the habitat sampled, the influence of different spatial scales varied between the seasons, and the effect of food resources appeared to be complex. These aspects must be taken into consideration for management decisions and restoration strategies to be effective.

Tropical freshwater ecosystems are understudied and not well understood relative to temperate systems; however, they are becoming increasingly imperiled by escalating anthropogenic impacts. The aim of this thesis was to investigate how tropical freshwater macroinvertebrate communities changed both structurally and functionally over a pristine-rural-urban land-use gradient, in relation to different spatial and temporal scales, as well as to the availability of potential food sources. Fifteen streams in the Panama Canal Watershed were sampled during the dry and wet seasons of 2007 and 2008, for macroinvertebrate communities (benthic and leaf litter), environmental variables, and potential food sources. Along the land-use gradient, in both habitat types, taxon richness, diversity, and evenness all decreased significantly; whereas, abundance increased significantly. For the benthic macroinvertebrate community, unique variation was explained equally well by local (water chemistry and sediment type) and landscape (riparian vegetation and watershed land use) characteristics in the dry season, and landscape characteristics explained slightly more variation in the wet season. Leaf-litter macroinvertebrate community unique variation was better explained by local variables than by landscape variables in both seasons. In terms of potential food resources, fine detritus and inorganic material were the most common across all streams (increased quantities in urban streams) and seasons; whereas, the availability of diatoms and leaf material increased in the dry season. Using gut content analyses, we found that collectors (gatherers and filterers) were by far the most common functional feeding group, increasing in abundance along the land-use gradient. Predators, shredders, and scrapers were all most abundant in pristine streams and decreased along the land-use gradient. Finally, using seven community metrices, a potential biomonitoring tool was developed, the Neotropical Low-land Stream Multimetric Index (NLSMI), which distinguished well among the different levels of stream impairment. This study demonstrated that tropical communities were negatively affected by human land alteration, but that community responses depended on the habitat sampled, the influence of different spatial scales varied between the seasons, and the effect of food resources appeared to be complex. These aspects must be taken into consideration for management decisions and restoration strategies to be effective.

Globally, human activities are altering nutrient biogeochemical cycles. The impact of humans on silicon (Si) cycles remains largely unexplored. Understanding the cycle of Si is important because weathering of siliceous rocks is a substantial sink of atmospheric carbon. Additionally, Si is required by diatoms. Diatoms form the base of important socioeconomic food webs, responsible for ~50% of oceanic net primary production, and deliver atmospheric carbon to ocean sediments as part of the ocean’s biological pump. My dissertation aims to assess the role of anthropogenic activities in altering Si cycling across the land-ocean continuum. Chapter 2 focuses on how assimilation of biogenic silica (BSi) by trees may be impacted by projected changes in climate. Using samples collected during a multi-year, snow removal experiment, I show that increased frequency and duration of soil freezing in winter significantly decreased (-28%) BSi in sugar maple (Acer saccharum) fine roots compared to control plots. Importantly, I observed that fine roots are a previously undescribed pool of BSi within sugar maples, accounting for 29% of total sugar maple BSi while only 4% of sugar maple biomass. Chapter 3 examines the origin and fate of Si within wastewater for the City of Boston. I determined the total dissolved silica (DSi) load in wastewater influent (69,500 kmol DSi year-1), then parsed the total DSi flux between Si contributions of sewage (49%), groundwater infiltration (39%), and surface runoff inflow (12%). In Chapter 4, I study the DSi load carried by treated effluent. I determined that effluent load (67,800 kmol DSi year-1) is not statistically different from influent load, indicating that wastewater treatment does not remove DSi. In Chapter 5 I demonstrate how humans impact concentrations of DSi in urban groundwater. Groundwater DSi increases with human presence and urban areas have significantly higher concentrations of DSi compared to groundwater conditions along the Massachusetts coast. I demonstrate that historic variables defining fill techniques, fill material, and pre-fill land-use out preform geologic variables in predicting urban groundwater DSi concentrations. This dissertation highlights human alterations to biological assimilation, fate, and effects of Si in sewage, and centuries-long subsurface Si impacts that perturb the distribution and availability of a nutrient intimately tied to water quality and climate.

Lina Mire, Gotland, is an area of archaeological significance and has a complex history of shoreline displacement. Archaeologists suspect that Lina Mire was once part of an important inland water system which connected the Littorina Sea with central Gotland. This study investigates vegetational and palaeoenvironmental changes of the Lina Mire area between 6900 – 400 BC (8850 – 2350 cal years BP) in order to better understand how the area has developed and how humans have impacted the vegetation. Pollen analysis, C/N ratios, organic matter and carbon content measurements were conducted. The chronology was based on 14C AMS dating of terrestrial macrofossils and bulk sediments. A transgression of the Littorina Sea at about 6550 BC (8500 cal years BP) inundated the Lina Mire basin, which was a lake at the time. The onset of cultivation was indicated by the presence of Hordeum (Barley or Wild Barley) during the Late Neolithic, 2630 BC (4580 cal years BP). Hordeum continued to grow during the Bronze Age when Cereals appeared at about 970 BC (2920 cal years BP). During the onset of cultivation during the Late Neolithic, the Lina Mire basin was a bay of the Littorina Sea. The Lina Mire basin remained connected with the Littorina Sea until isostatic uplift caused it to become isolated at about 1870 BC (3820 cal years BP). The lake overgrew and became a mire about 820 BC (2770 cal years BP).

The red-cockaded woodpecker (Picoides borealis) is a federally endangered species. As such, populations need to be increased in order to achieve recovery goals outlined by the U.S. Fish and Wildlife Service. My thesis is composed of two chapters that represent opposite sides of this issue. The first chapter investigates whether military training activity negatively affects red-cockaded woodpeckers. Military installations in the southeastern United States contain several of the largest remaining red-cockaded woodpecker populations. Six of the 15 installations harboring these birds are designated primary core populations; thus, population increases on these sites are critical to recovery of the species. However, restrictions on military training activity associated with red-cockaded woodpecker protection are a cause of concern on military installations that sometimes constrains management for population growth. Current restrictions are based on assumptions of potential impacts rather than scientific evidence, so we evaluated two different restriction regimes to test for training activity effects. The second chapter concerns how to induce populations to grow more rapidly through natural processes. As a cooperative breeder, red-cockaded woodpeckers preferentially compete for existing breeding positions and queue in the form of helping or floating to obtain a breeding vacancy, rather than create new territories. I used 20 years of demographic data collected as part of a long-term monitoring study of red-cockaded woodpeckers to investigate mechanisms that stimulate territory creation in this cooperatively breeding species. / Master of Science

Streamflow characteristics change over time as a result of water resources development and management projects, water use, watershed land use changes, and climate changes. The main objective of this thesis is to assess the significance of the impacts of human activities such as construction of reservoirs, water supply diversions, increased water use and return flows on streamflows by the recently completed Texas WAM (Water Availability Modeling) system. The major river basins in the state of Texas were selected as suitable study basins. The particular objective is accomplished by the assessment of WAM monthly and annual naturalized and regulated flows, based on using the WRAP (Water Rights Analysis Package) model, which represents the river/reservoir management model. WAM flow frequency analysis was performed for the simulated flows. The flow ratio indices developed showed the divergence of the actual flows from their natural behavior for the entire monthly flow frequency flow spectrum ranging from minimum flows to high flows. This study describes the combined effects of reservoir construction, increased water use, water resources development projects and land use changes on the river flow regime.

Subsurface solutional pathways make limestone terrains sensitive to changes in soil properties that regulate flows to the epikarst. This study examines biogeomorphic factors responsible for changed water movements and erosion in fluviokarst slopes deforested 200 years ago along the Kentucky River, Kentucky. In this project, infiltration and water content data from forest and fescue grass soil profiles were analyzed within a detailed overview of system factors regulating hillslope hydrology. Results show that grass has growth and rooting characteristics that tend to create a larger volume of lateral water movement in upper soil layers than occurs under forests. This sets up the current emergent pattern of erosion in which water perches at grass slope bases and overwhelms pre-existing epikarst drainage. Tree roots are able to cause solution at multiple discrete points of entry into fractures and bedding planes, increasing storage capacity and releasing sediment over time. Grass roots do not enter bedrock, and their rooting depth limits diffuse vertical preferential flow in root channels to above one meter. In the areas dense clay soils, flow under grass is conducted sideways either through the regolith or at the bedrock surface. Rapid flow along rock faces in hillslope benches likely moves fines via subsurface routes from the hillslope shoulders, causing the exposure of flat outcrops under grass. Lower growing season evapotranspiration also promotes higher grass summer flow volumes. Gullying occurs at sensitive points where cutters pass from the uphill grassed area into the forest, or where flow across the bedrock surface crosses grass/forest boundaries oriented vertical to the slope. At these locations, loss of the protective grass root mat, coupled with instigation of tree root preferential flow in saturated soils, causes soil pipes to develop. Fluviokarst land management decisions should be based on site-specific slope, soil depth, and epkarst drainage conditions, since zones sensitive to erosion are formed by spatial and temporal conjunctions of a large number of lithologic, karst, soil, climate, and vegetation factors. This study shows that it is the composite of differing influences created by forest and grass that make forests critical for soil retention in high-energy limestone terrains.

Dissolved organic carbon (DOM) is one of the largest cycled organic carbon pools on Earth and an important biogeochemical factor in aquatic systems. DOM can act as an energy source for microorganisms, alter the depth of the photic zone for photosynthesis, absorb harmful ultraviolet radiation, as well as alter the transport and toxicity of contaminants. The purpose of this research project was to characterize DOM in the Grand River watershed in Ontario, Canada using a wide range of qualitative and quantitative techniques and determine the impact of anthropogenic activities as well as seasonal and longitudinal changes on DOM processes. To reach the study objectives, historical data was analyzed to determine the seasonal cycle in the Grand River watershed. Intensive longitudinal sampling surveys were undertaken to evaluate the DOM characteristics and processes in the Grand River. Surveys of the less impacted Burnt River watershed were used as a comparison watershed to the Grand River to evaluate allochthonous and autochthonous indicators of DOM source and human impacts on DOM processes. Drinking water surveillance data was used to evaluate the effect of DOM in the Grand River on formation of disinfection by-products (DBPs). Different trends were seen in the Grand River in terms of longitudinal area and season. The headwaters of the river showed more autochthonous DOM in the spring and winter compared to the fall and summer. The lower-central river peaked in autochthonous DOM in the summer and was more allochthonous in the winter. DOM generally became more autochthonous downstream in the Grand River and was most autochthonous below the large sewage treatment plants (STPs) in the central portion. Protein content, measured as protein-like fluorescence normalized to DOC concentration, was strongly related to ??15N of DON; both are associated with autochthonous DOM in the Grand River and show the effects of the major STPs. The increase in autochthonous DOM below the STPs is likely associated with nutrient enrichment stimulating primary production and macrophyte growth. Based on the comparison of the Burnt River with the more impacted Grand River, the effect of lakes and photodegradation can make discrimination of autochthonous and allochthonous DOM more difficult. The ratio of DOC/DON and protein-like fluorescence proved to be robust indicators despite photodegradation. Human impacts on the Grand River watershed result in a greater seasonal cycle, high primary production in the summer and a downstream trend of increasing autochthonous DOM compared to the Burnt River. Based on drinking water surveillance data and literature review, autochthonous DOM caused greater DBPs in the drinking waters fed by the Grand River. This is currently a threat to human health and DBPs in sewage treatment plant effluent may be a threat to ecosystem health.

Submitted by Jean Medeiros (jeanletras@uepb.edu.br) on 2017-02-09T13:57:16Z No. of bitstreams: 1 PDF - Climélia da Nóbrega Silva.pdf: 2378682 bytes, checksum: bc27dc4732d9c3146e265bcaaa7cece6 (MD5) / Made available in DSpace on 2017-02-09T13:57:16Z (GMT). No. of bitstreams: 1 PDF - Climélia da Nóbrega Silva.pdf: 2378682 bytes, checksum: bc27dc4732d9c3146e265bcaaa7cece6 (MD5) Previous issue date: 2014-12-02 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Estuaries are environments that run important ecosystem services, such as nurseries and feeding places for numerous species. However, are subject to various anthropogenic impacts that directly affect their operation. This study aimed to test which taxonomic level is sufficient to evaluate the environmental condition as well as assess the relative efficacy of ecological indicators in estuaries assumed as subject to anthropogenic pressures of different intensities. The research was conducted in the estuaries of the River Paraíba do Norte and Mamanguape River (Northeastern Brazil), in November 2013, totaling 27 sampling sites, parted into four zones according to the values of salinity, sediment composition and depth. The organisms collected were sorted and identified to the lowest possible taxonomic level. This study is divided into two chapters, the first taxonomic depicts sufficiency by addressing the hypothesis that the family level would be more efficient to detect the environmental condition and the second test whether the combined use of the thermodynamically oriented indicators, functional trophic groups wealth rate and diversity index Shannon-Wiener would provide consistent answers in the evaluation of the ecological condition of estuaries. Fill Taxonomic showed that for the purpose of this study, both the family level as gender were enough to detect changes in environmental conditions, for this sample period. Regarding the use of associated ecological indicators to detect changes in ecological conditions of the estuaries, the indicators based on taxa richness, diversity, specific eco- exergy and functional feeding groups did not respond concurrently in the detection of changes in the ecological condition of estuaries with different levels of human impacts. Only eco-exergy index indicated differences in the ecological condition of the assessed ecosystems. Thus, the results obtained from the survey showed the importance of the study on the taxonomic sufficiency and ecological indicators based on benthic communities as a biomonitoring tool, which aims to understand the variations and fluctuations of ecosystems as well as the basis for measures proposed to mitigation of human impacts, conservation and environmental management. / Os estuários são ambientes que prestam serviços ecossistêmicos importantes, como berçários e locais de alimentação para inúmeras espécies. Porém, estão sujeitos a diversos impactos antrópicos que afetam diretamente seu funcionamento. O objetivo do trabalho foi testar qual nível taxonômico é suficiente para avaliar a condição ambiental, assim como verificar a eficácia relativa de indicadores ecológicos em estuários assumidos como sujeitos às pressões antrópicas de diferentes intensidades. A pesquisa foi realizada nos Estuários do Rio Paraíba do Norte e do Rio Mamanguape (Nordeste brasileiro), em novembro de 2013, totalizando 27 pontos de amostragem, divididos em quatro zonas de acordo com os valores de salinidade, composição do sedimento e profundidade. Os organismos coletados foram triados e identificados ao menor nível taxonômico possível. Este estudo está dividido em dois capítulos, o primeiro retrata a suficiência taxonômica, abordando a hipótese de que o nível de família seria mais eficiente em detectar a condição ambiental e o segundo testou se o uso associado dos indicadores termodinamicamente orientados, grupos tróficos funcionais, riqueza de taxa e índice de diversidade de Shannon-Wiener forneceriam respostas concordantes na avaliação da condição ecológica dos estuários. A suficiência taxonômica demonstrou que, para o objetivo desta pesquisa, tanto o nível de família quanto o de gênero foram suficientes em detectar alterações nas condições ambientais, para este período amostral. Em relação à utilização associada de indicadores ecológicos para detectar as mudanças nas condições ecológicas dos estuários, os indicadores baseados na riqueza de taxa, diversidade, eco-exergia específica e grupos tróficos funcionais não responderam de forma concordante na detecção das variações na condição ecológica dos estuários com níveis de impactos antrópicos distintos. Apenas o índice da eco-exergia indicou diferenças na condição ecológica dos ecossistemas avaliados. Assim, os resultados obtidos pela pesquisa evidenciaram a importância do estudo sobre a suficiência taxonômica e indicadores ecológicos baseados nas comunidades bentônicas como um instrumento de biomonitoramento, cujo intuito é entender as variações e flutuações dos ecossistemas, assim como servir de base para propostas de medidas para mitigação dos impactos antrópicos, conservação e gestão ambiental.