Hydraulic conditions (velocity, depth, turbulence) strongly influence the distribution and abundance of organisms in rivers. A diverse hydraulic environment should foster biodiversity, because organisms have different hydraulic preferences. In fact, the relationship between spatial hydraulic diversity and biodiversity is largely presumed, and not well-supported by empirical studies, but it underpins efforts in river restoration and conservation. This is particularly so at the reach scale, indicating a stream- or river-section with large-scale homogeneous geomorphic and hydrological conditions and smaller-scale habitat patches, as perceived by organisms in the community under study. This thesis considers the factors that create spatial hydraulic diversity, and the ways that fish respond. It presents a method to characterise hydraulic diversity, and uses this to describe temporal and spatial changes between reaches. It also demonstrates the use of hydraulic modelling for comparing reaches. Finally, it assesses the Acoustic Doppler Current Profiler (ADCP) as a method to describe hydraulic conditions in a large, open river channel. Swimming ability tests were applied to three small freshwater fish, the pelagic Australian smelt (Retropinna semoni) and common galaxias (Galaxias maculatus ) and the demersal flathead gudgeon (Philypnodon grandiceps). The latter species was the weaker swimmer, but the tests indicated that behaviour also should be considered. A laboratory experiment was designed to investigate how two species with contrasting ecological habits (common galaxias, flathead gudgeon) behave in a diverse hydraulic environment. Habitat choices and activity were monitored in a constructed sinuous channel at three discharges over a 3-hour period. The galaxias favoured the pelagic habitat, and spent 20-60% of the time cruising, whereas the flathead gudgeon preferred the demersal habitat and spent <6% of the time cruising. The flathead gudgeons could access their preferred habitat at all discharges, but the common galaxias were limited by their swimming ability at the highest discharge. Several methods to characterise reaches were compared for eight 3-D model reaches representing the effects of channel form, wood and aquatic plants. The variogram (a measure of the variance between samples as a function of distance) emerged as a superior method because it indicates hydraulic diversity, incorporates the spatial arrangement of hydraulic patches, and facilitates comparisons between reaches. The ADCP proved a quick, reliable means to measure depth and 3-D velocity in rivers. It was effective only in depths >1.5 m, but modified instrumentation may overcome this limitation. Six reaches, including weir-pool and free-flowing sections, were compared at two discharges in the River Murray, Australia. Variograms derived from the ADCP data clearly demonstrated spatial differences between the sections, but temporal differences were less well-defined, suggesting that reaches may retain characteristic hydraulic patterns despite changes in discharge. Opportunities for further research include: the issue of optimal levels of hydraulic diversity for fish and other biota; use of variograms as a tool for field studies of aquatic biota; and measuring reach-scale hydraulic diversity and biodiversity before and after reach manipulation (e.g. the placement of wood), to elucidate the effects of changes in spatial hydraulic diversity on reach biodiversity. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1344602 / Thesis (Ph.D.) - University of Adelaide, School of Earth and Environmental Sciences, 2008

Heart disease is the developed world's biggest killer, and the shortage of donor hearts has accelerated the development of mechanical alternatives. Scientists, engineers and clinicians have attempted to replicate the human heart with a mechanical device for over 50 years. Although a number of pulsating devices have been developed, and in some cases worked briefly, they have invariably failed to match the success of heart transplantation. In an attempt to produce a suitable alternative, current research is focused on devices that do not replace the heart; but rather work along side it to assist its function. Many of these devices help the failing left ventricle; however some patients require the additional implantation of a second device to assist a failing right ventricle. This increases implantation time and associated risk, and because of the size of the current devices, reduces the access of smaller patients to this vital technology. The overall thesis objective focuses on the progressive design, development and preliminary evaluation of two novel centrifugal type ventricular assist devices, a bi-left ventricular device (Bi-LVAD) and a single bi-ventricular assist device (Bi-VAD). The devices have the respective capability to assist either the left ventricle, or both ventricles of a failing heart. The current concept for each VAD employs both magnetic and hydrodynamic suspension techniques to float a rotating double impeller, a technique that aims to reduce blood damage and component wear, two of the major problems encountered with current generation devices. Each VAD design was developed by conducting experimentation and drawing conclusions from a variety of engineering research fields, such as flow visualization, rotary pump design and testing, fluid dynamics, hemodynamics and heart failure, and magnetic motor bearing design. In order to evaluate pump prototype designs, it was necessary to design and develop a novel pulsatile systemic and pulmonary mock circulation loop capable of reproducing the hemodynamics of heart failure in the systemic and pulmonary circuits. The investigation then specifically examined the static hydraulic forces on the impeller of a centrifugal blood pump during operation in this mock circulation loop. The recorded magnitude and direction of radial and axial thrust then influenced the selection of magnetic and hydrodynamic bearing configurations to minimise impeller touchdown in the intended hemodynamic environment. This research required the development of correctly designed impeller (semi-open/closed) and volute (single, double, circular) components for each ventricular assist application and a unique test facility to isolate impeller hydraulic forces in addition to the mock circulation loop. The proposed Bi-LVAD incorporates symmetrical blade designs on each side of the double sided impeller. The device assists the function of the left ventricle only with symmetrical axial pressure distribution and elimination of stagnant regions beneath the impeller. These features improve axial touchdown capacity and reduce thrombus formation respectively. The proposed Bi-VAD incorporates different blade designs on each side of the double impeller to augment the function of both the left and right cardiac chambers. The design has the additional potential to act as a total artificial heart (TAH). To date there is no Bi-VAD/TAH system available that incorporates an LVAD and RVAD in one rotary pump. Successful development of each innovative VAD will provide an alternative to heart transplantation, potentially saving lives of many terminal heart patients each year. No longer would heart transplant candidates need to wait for the untimely death of a donor to provide a suitable heart. Instead, this new generation device would be available immediately, and be almost universally compatible with all patients. It has the potential to dramatically increase a patient’s expected lifetime, and to deliver them a higher quality of life.

Acacia holosericea A. Cunn. Ex G. Don (Velvet Leaf Wattle, Family Mimosaceae) is indigenous to Australia and holds promise as a novel cut foliage crop due to its silvery green silky phyllodes. Insufficient water uptake, possibly due to low stem hydraulic conductivity (Kh), is potentially responsible for early wilting and desiccation of phyllodes and limiting vase life. This study aimed to characterize the anatomy of stem xylem conduits and determine cation (K+ and Ca2+) mediated stem Kh. Differential localization of Ca2+ in xylem vessels and the effects of KCl and CaCl2 salts as cation contributors in vase solutions were also evaluated for their effects on cut foliage longevity. Anatomical characteristics of stem xylem conduits were studied using light, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Tracheids and vessels with simple perforation plates were the principal water conducting cells. SEM and TEM revealed bordered vestured intervessel pits in xylem conduits. Vestures were branched and coralloid-like structures. Xylem conduit lengths were assessed by ink perfusion. The majority of conduits (89%) were short (1 to 5 cm), and only ~ 2% were long (15 to 16 cm). Mean conduit diameter was 77 ± 0.9 µm, with 29% of conduits in the range 70 to 80 µm. Effects of S-carvone (0, 0.318, and 0.636 mM), a monoterpene inhibitor of wound-healing enzyme inhibitor found in caraway (Carum carvi) and dill (Anethum graveoleus) seeds, on several native Australian, non-proteaceous cut flower and foliage species including A. holosericea (Mimosaceae), Baeckea frutescens (Myrtaceae), Chamelaucium uncinatum cv. ‘Mullering Brook’ (Myrtaceae), and the non-native Chrysanthemum sp. cv. ‘Dark Splendid Reagan’ (Asteraceae) were examined. As comparator treatments regular recutting of stem ends and use of standard tap water (STW: 0.7 mM CaCl2, 1.5 mM NaHCO3, and 0.05 mM CuSO4 in vases) were tested. S-Carvone treatments significantly (P≤0.05) extended the vase life of B. frutescens and C. uncinatum, constituting the first report of positive S-carvone effects on the vase life of Myrtaceous species. S-Carvone at 0.318 and 0.636 mM did not have antibacterial effects against Bacillus cereus (the main vase solution microbe) either in vitro or in the vase solution. Regular recutting of stem ends consistently improved all vase life parameters [viz. relative fresh weight (RFW), solution uptake, and vase life] in the three native species examined. STW had a positive effect on RFW and solution uptake only for A. holosericea cut foliage. Effects of di- and monovalent cations (Ca2+ as CaCl2 and K+ as KCl) on stem Kh of cut stem segments were studied. Abundance of Ca2+ on pit membranes versus xylem lumen wall surfaces was investigated using a novel low vacuum (LV) SEM plus energy dispersive X-ray (EDX) microanalysis technique. Both salts (0.1, 1, 10, and 100 mM KCl or CaCl2) did not significantly increase stem Kh compared to the corresponding deionised (DI) water controls (experiment 1). Highest increase in Kh was with KCl and CaCl2 at 10 and 1 mM, respectively. Increases in Kh with 100 mM KCl and CaCl2 were significant over DI water (experiment 2) for long (10 and 20 cm) and short (2 and 5 cm) stem segments, respectively. Increases in Kh of 1.2- and 2.4-fold for 100 mM KCl over DI water were found with increasing stem length from 2 to 20 cm. Kh decreased as stem segment length increased from 5 to 20 cm. However, contrasting results were found with 100 mM CaCl2, where ΔKh was larger in shorter (2 cm) than longer (20 cm) segments. To prevent dislocation of ions and distortion damage to the specimens, stem pieces were first LV freeze-dried, and then carbon-coated, viewed under SEM, and analysed for elemental composition and distribution by EDX. However, the method could not identify specific calcium peaks in xylem vessels perhaps because background signals were too high, and tissue topography interfered with signal detection. Effects of KCl and CaCl2 on vase life were also tested. RFW, solution uptake, and vase life were higher with 10 mM KCl and CaCl2 in the vase solution than with 0, 1, and 100 mM. STW had a significant (P≤0.05) positive effect on RFW and solution uptake rate when tested against deionised water, 10 mM KCl and 10 mM CaCl2. Different combinations of 0.05 mM CuSO4, 10 mM CaCl2, and 10 mM KCl were also tested as vase solutions. A significant positive effect on RFW and vase life was obtained with CuSO4 alone, CaCl2 alone, and CuSO4 plus KCl. Only CuSO4 and CaCl2 gave a significant positive effect on solution uptake rate. None of the eight treatments tested showed a consistent effect on stomatal conductance or stem Kh. Overall, the research revealed that tracheids and vessels with simple perforation plates and bordered vestured intervessel pits are the principal water conducting cells in A. holosericea. LV-SEM-EDX technique was unable to assess the spatial distribution of Ca2+ on xylem vessels, but this was the first attempt to apply this technique. Also, this is the first report on the extension of vase life of B. frutescens and C. uncinatum (Myrtaceous species) using S-carvone. In vase solutions, 0.05 mM CuSO4, 10 mM KCl plus 0.05 mM CuSO4 and 10 mM CaCl2 should have positive influences on the water balance of A. holosericea cut foliage stems.

Hydrological observations are characterised by wet and dry cycles, a characteristic that is termed hydrological persistence. Interactions between global climate phenomena and the hydrological cycle result in rainfall and streamflow data clustering into wetter and drier states. These states have implications for the management and planning of water resources. Statistical tests constructed from the theory of wet and dry spells indicate that evidence for persistence in monthly observations is more compelling than at an annual scale. This thesis demonstrates that examination of monthly data yields spatially - consistent patterns of persistence across a range of hydrological variables. It is imperative that time series models for rainfall and streamflow replicate the observed fluctuations between the climate regimes. Monthly time series are generally represented with linear models such as ARMA variants ; however simulations from such models may underestimate the magnitude and frequency of persistence. A different approach to modelling these data is to incorporate shifting levels in the broader climate with a tendency to persist within these regimes. Hidden Markov models ( HMMs ) provide a strong conceptual basis for describing hydrological persistence, and are shown to provide accurate descriptions of fluctuating climate states. These models are calibrated here with a full Bayesian approach to quantify parameter uncertainty. A range of novel variations to standard HMMs are introduced, in particular Autoregressive HMMs and hidden semi - Markov models which have rarely been used to model monthly rainfall totals. The former model combines temporal persistence within observations with fluctuations between persistent climate states, and is particularly appropriate for modelling streamflow time series. The latter model extends the modelling capability of HMMs by fitting explicit probability distributions for state durations. These models have received little attention for modelling persistence at monthly scale. A non - parametric ( NP ) HMM, which overcomes the major shortcomings of standard parametric HMMs, is also described. Through removing the requirement to assume parametric forms of conditional distributions prior to model calibration, the innovative NP HMM framework provides an improved estimation of persistence in discrete and continuous data that remains unaffected by incorrect parametric assumptions about the state distributions. Spatially - consistent persistence is identified across Australia with the NP HMM, showing a tendency toward stronger persistence in low-rainfall regions. Coherent signatures of persistence are also identified across time series of total monthly rainfall, numbers of rain - days each month, and the intensities of the most extreme rain events recorded each month over various short durations, illustrating that persistent climate states modulate both the numbers of rain events and the amount of moisture contained within these events. These results provide a new interpretation of the climatic interactions that underlie hydrological persistence. The value of HMMs to water resource management is illustrated with the accurate simulation of a range of hydrologic data, which in each case preserves statistics and spell properties over a range of aggregations. Catchment - scale rainfall for the Warragamba Reservoir is simulated accurately with HMMs, and rainfall - runoff transformations from these simulations provide reservoir inflows of lower drought risk than provided from ARMA models. / Thesis (Ph.D.)--School of Civil and Environmental Engineering, 2006.

Hydraulic conductance of roots of the grapevine cultivar, Chardonnay, varies diurnally, peaking at 1400 h. The diurnal amplitude of hydraulic conductance between 600 and 1400 h was not altered when potted grapevines were water-stressed by withholding water for 8 days. However, the diurnal change was greatly reduced for water-stressed Grenache. If the diurnal change in root hydraulic conductance is a result of changes in aquaporin gene expression or activity, it suggests that aquaporins respond differently in water-stressed Chardonnay and Grenache roots. Both Chardonnay and Grenache demonstrated a reduction in hydraulic conductance in response to water stress, with Grenache exhibiting a larger reduction. Suberisation of the roots increased in response to water stress, with complete suberisation of the endodermis occurring closer to the root tip of Grenache compared to the more drought sensitive Chardonnay. The drought sensitive rootstock, 101-14 (V. riparia × V. rupestris) demonstrated a similar reduction in hydraulic conductance to Chardonnay, while drought tolerant 1103 Paulsen (V. berlandieri × V. rupestris) had a non-significant reduction when water-stressed compared to the large reduction observed for drought tolerant Grenache. Therefore, in this study the degree of reduction in hydraulic conductance did not relate to the drought tolerance of the four varieties examined. The impact of partial drying (watering only half the root system) on hydraulic conductance also differed between Chardonnay and Grenache. There was no change in the conductance of the whole root system of Chardonnay due to an increase in conductance of the roots in the wet half which compensated for the reduction on the dry side. In contrast, Grenache did suffer a reduction measured over the whole root system due to a much larger reduction on the dry side compared to Chardonnay. There was an increase in hydraulic conductance on the wet side but this could not compensate for the large reduction on the dry side. Two aquaporins (VvPIP1;1 and VvPIP2;2) were cloned from the roots of grapevine cultivar Chardonnay. The genes were expressed in Xenopus oocytes to determine their osmotic permeability. As has been shown in a number of plant species, VvPIP1;1 was only slightly permeable to water, whereas VvPIP2;2 did transport water. However, when VvPIP1;1 was injected into the oocytes with VvPIP2;2, there was a substantial increase in the osmotic permeability. There was no significant variation in the diurnal expression of VvPIP2;2, whereas VvPIP1;1 showed a peak in expression at 1000 h prior to the peak in hydraulic conductance and peaked again at 1800 h. VvPIP2;2 did not vary in transcript level in response to water stress or rewatering in Chardonnay or Grenache roots. The level of VvPIP1;1 doubled in water stressed Chardonnay roots and declined again when the vines were rewatered 24 h previously. This response to water stress did not occur in Grenache roots. The roots used were from the apical 5 cm. Similar roots were used to measure the water permeability of the cortical cell membranes using the cell pressure probe. Changes in cell membrane permeability in response to water stress corresponded to changes in VvPIP1;1 expression. An experiment to determine if shoot topping had an effect on root hydraulic conductance revealed a significant 50% decline. This response was also observed in soybean (Glycine max L.) and maize (Zea mays L.). A range of experiments have been performed to determine the reason for the decline. Possibilities included a response to final leaf area and reduced transpirational demand; loss of a carbohydrate sink; or hormonal signals such as abscisic acid, auxin and ethylene. At this stage the nature of the positive or negative signal that causes the change in root hydraulic conductance remains elusive. However, the signal did cause a reduction in the transcript level of VvPIP1;1, indicating the involvement of aquaporins in the response. The root hydraulic conductance of grapevines is variable and dependent on factors such as time of day, water-stress, transpiration rate and unknown signals from the shoot. A proportion of this variability is due to changes in aquaporin number or activity. There are also genotypic differences which may be beneficial for future breeding efforts to improve water use efficiency of grapevines. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1311202

Hydraulic conductance of roots of the grapevine cultivar, Chardonnay, varies diurnally, peaking at 1400 h. The diurnal amplitude of hydraulic conductance between 600 and 1400 h was not altered when potted grapevines were water-stressed by withholding water for 8 days. However, the diurnal change was greatly reduced for water-stressed Grenache. If the diurnal change in root hydraulic conductance is a result of changes in aquaporin gene expression or activity, it suggests that aquaporins respond differently in water-stressed Chardonnay and Grenache roots. Both Chardonnay and Grenache demonstrated a reduction in hydraulic conductance in response to water stress, with Grenache exhibiting a larger reduction. Suberisation of the roots increased in response to water stress, with complete suberisation of the endodermis occurring closer to the root tip of Grenache compared to the more drought sensitive Chardonnay. The drought sensitive rootstock, 101-14 (V. riparia × V. rupestris) demonstrated a similar reduction in hydraulic conductance to Chardonnay, while drought tolerant 1103 Paulsen (V. berlandieri × V. rupestris) had a non-significant reduction when water-stressed compared to the large reduction observed for drought tolerant Grenache. Therefore, in this study the degree of reduction in hydraulic conductance did not relate to the drought tolerance of the four varieties examined. The impact of partial drying (watering only half the root system) on hydraulic conductance also differed between Chardonnay and Grenache. There was no change in the conductance of the whole root system of Chardonnay due to an increase in conductance of the roots in the wet half which compensated for the reduction on the dry side. In contrast, Grenache did suffer a reduction measured over the whole root system due to a much larger reduction on the dry side compared to Chardonnay. There was an increase in hydraulic conductance on the wet side but this could not compensate for the large reduction on the dry side. Two aquaporins (VvPIP1;1 and VvPIP2;2) were cloned from the roots of grapevine cultivar Chardonnay. The genes were expressed in Xenopus oocytes to determine their osmotic permeability. As has been shown in a number of plant species, VvPIP1;1 was only slightly permeable to water, whereas VvPIP2;2 did transport water. However, when VvPIP1;1 was injected into the oocytes with VvPIP2;2, there was a substantial increase in the osmotic permeability. There was no significant variation in the diurnal expression of VvPIP2;2, whereas VvPIP1;1 showed a peak in expression at 1000 h prior to the peak in hydraulic conductance and peaked again at 1800 h. VvPIP2;2 did not vary in transcript level in response to water stress or rewatering in Chardonnay or Grenache roots. The level of VvPIP1;1 doubled in water stressed Chardonnay roots and declined again when the vines were rewatered 24 h previously. This response to water stress did not occur in Grenache roots. The roots used were from the apical 5 cm. Similar roots were used to measure the water permeability of the cortical cell membranes using the cell pressure probe. Changes in cell membrane permeability in response to water stress corresponded to changes in VvPIP1;1 expression. An experiment to determine if shoot topping had an effect on root hydraulic conductance revealed a significant 50% decline. This response was also observed in soybean (Glycine max L.) and maize (Zea mays L.). A range of experiments have been performed to determine the reason for the decline. Possibilities included a response to final leaf area and reduced transpirational demand; loss of a carbohydrate sink; or hormonal signals such as abscisic acid, auxin and ethylene. At this stage the nature of the positive or negative signal that causes the change in root hydraulic conductance remains elusive. However, the signal did cause a reduction in the transcript level of VvPIP1;1, indicating the involvement of aquaporins in the response. The root hydraulic conductance of grapevines is variable and dependent on factors such as time of day, water-stress, transpiration rate and unknown signals from the shoot. A proportion of this variability is due to changes in aquaporin number or activity. There are also genotypic differences which may be beneficial for future breeding efforts to improve water use efficiency of grapevines. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1311202

Local scour around bridge piers can cause serious damages and structural failure to the bridge. Correct prediction of the scour is an important criterion for the engineering design. Though the subject has been investigated for many decades, the theoretical developments have been very limited due to the complicated interaction of three-dimensional flow and the sediment transport. This thesis concerns the flow around a vertical bottom mounted cylinder exposed to currents and is considered as the initial phase of a study towards modeling local scour around vertical bottom-mounted structures. The aim of the present study is to obtain a better understanding of the complex three-dimensional flow and the mechanisms related to the scouring. The study started with the development of a three-dimensional numerical model to simulate flow around cylindrical structures. After validation of the model, the model was applied to investigate flow around an isolated rectangular cylinder. Unsteady flows around cylinders of a square cross section (A/D = 1) and a rectangular cross section (A/D = 2) were simulated to understand the flow properties around a cylinder other than a circular cross section. Three-dimensional flow patterns, pressure distribution, forces on the cylinder and vortex-shedding frequencies were discussed. It was found that the present numerical results generally agree well with the experimental data. Flow around a vertical cylinder mounted on a rigid bed was then investigated by the numerical model. A circular and a square cross sections were considered respectively. Flow structures of horseshoe vortex and the wake vortex which are the major mechanisms leading to the scouring around the base of the vertical cylinder were explored. The bed shear stress distributions that directly affect the scour processes were discussed. Finally the numerical model was applied to study the flow around a submerged square cylinder mounted on a bed, which has significant engineering relevance to the local scour around structures, such as bridge pier foundation itself, or a caisson placed underwater around the pier bottom for scour protection. The numerical calculations were carried out at different cylinder heights to investigate the effect of the cylinder height on the flow properties. It was found that for submerged cylinders with the height of less than one and a half of the cylinder side width the maximum bed shear stress amplification is about 60% of the value of an infinite long cylinder. The quantification of the shear stress reduction is important for scour protection design.

Thesis (M.S. - Hydrology and Water Resources)--University of Arizona. / Includes bibliographical references.

Thesis (Ph. D.)--Ohio State University, 2007. / Title from first page of PDF file. Includes bibliographical references (p. 202-207).

Thesis (M. S.)--Mechanical Engineering, Georgia Institute of Technology, 2009. / Committee Chair: Book, Wayne; Committee Member: Lipkin, Harvey; Committee Member: Paredis, Christiaan