Objectives: The purpose of this study was to evaluate whether the effects of hyperbaric oxygen (HBO) therapy could alter the critical size defect (CSD) diameter and to evaluate the effect of HBO on the repair of CSD in the presence and absence of a non-vascularized autogenous bone graft (ABG). Study Design: Twenty rabbits were divided in two groups of ten animals each. CSD were created in the parietal bones bilaterally. Defects were critical-sized, 15 mm on one side and supracritical-sized, 18 mm on the contralateral side. Group 1 received a 90-min HBO treatment sessions at 2.4 absolute atmospheric pressure (ATA) for 90 minutes per day for 20 days. Group 2 served as a normobaric roomair control (NBO). Additional ten animals were divided into 2 groups of 5 animals each. Bilateral CSD were created. ABG were allocated to one side of each calvarium. Group 1 received HBO treatments. Group 2 served as NBO. After sacrifice, data were collected including qualitative assessment, radiographic analysis, Micro CT bone analysis and histomorphometric analysis. ANOVA and paired sample t test were used for statistical analysis. Results: Both radiographic analysis and histomorphometric analysis demonstrated more new bone in the HBO CSD (p<.001). Micro CT analysis indicated a higher bone mineral content (BMC) in ABG CSD (p<.05). Histologically, complete bridging of the defect was observed in ABG defects. Histomorphometric analysis showed that HBO treatment increased new bone and marrow and reduced fibrous tissue in the defects (p<.01 for all). Conclusion: Bone regeneration was significantly greater in the HBO animals regardless of the defect size. HBO may have changed the accepted diameter of CSD to more than 18 mm. HBO enhances bony healing in non-grafted CSD.

Objectives: The purpose of this study was to evaluate whether the effects of hyperbaric oxygen (HBO) therapy could alter the critical size defect (CSD) diameter and to evaluate the effect of HBO on the repair of CSD in the presence and absence of a non-vascularized autogenous bone graft (ABG). Study Design: Twenty rabbits were divided in two groups of ten animals each. CSD were created in the parietal bones bilaterally. Defects were critical-sized, 15 mm on one side and supracritical-sized, 18 mm on the contralateral side. Group 1 received a 90-min HBO treatment sessions at 2.4 absolute atmospheric pressure (ATA) for 90 minutes per day for 20 days. Group 2 served as a normobaric roomair control (NBO). Additional ten animals were divided into 2 groups of 5 animals each. Bilateral CSD were created. ABG were allocated to one side of each calvarium. Group 1 received HBO treatments. Group 2 served as NBO. After sacrifice, data were collected including qualitative assessment, radiographic analysis, Micro CT bone analysis and histomorphometric analysis. ANOVA and paired sample t test were used for statistical analysis. Results: Both radiographic analysis and histomorphometric analysis demonstrated more new bone in the HBO CSD (p<.001). Micro CT analysis indicated a higher bone mineral content (BMC) in ABG CSD (p<.05). Histologically, complete bridging of the defect was observed in ABG defects. Histomorphometric analysis showed that HBO treatment increased new bone and marrow and reduced fibrous tissue in the defects (p<.01 for all). Conclusion: Bone regeneration was significantly greater in the HBO animals regardless of the defect size. HBO may have changed the accepted diameter of CSD to more than 18 mm. HBO enhances bony healing in non-grafted CSD.

The present research sought to understand patient experiences during Hyperbaric Oxygen Therapy (HBOT) by using 24 HBOT patients (17 men, 7 women) to examine the relationship between individual variables and anxiety, and providing One Session Exposure Therapy (OSET; Öst, 1989) if necessary. Pre-HBOT participants completed the following measures: State-Trait Anxiety Inventory (STAI; Spielberger, 1983), Claustrophobia Questionnaire (CLQ; Radomsky, Rachman, Thordarson, McIsaac, & Teachman, 2001), Anxiety Sensitivity Index (ASI; Reiss, Peterson, Gursky, & McNally, 1986), and Treatment Credibility/Expectancy Questionnaire (CEQ; Devilly & Borkovec, 2000). State Anxiety was assessed pre-HBOT and at the tenth and last sessions. Findings suggest Dispositional Anxiety (STAI-Trait + ASI), Expectancy of symptom improvement (CEQ), and gender were significantly predictive of State Anxiety before and during HBOT. Limitations and directions for future research are discussed.

Hyperbaric oxygen therapy is a treatment method that involves inhalation of 100% oxygen, and under higher pressure than the atmospheric. Blood has the ability to deliver more oxygen authorities. Treatment takes place in facilities called the hyperbaric chamber. During treatment, patients must adhere to certain principles that are given by the doctors and nurses working in these workplaces.The main objectives of this thesis were to map the influence of preparation on the course of treatment in a hyperbaric chamber and to determine how the patients are instructed by nurses during treatment in the hyperbaric chamber. Three research questions were set up to clarify how the preparation affects the patient during treatment in a hyperbaric chamber, as the patient is educated before treatment and what output the patient receives after leaving the hyperbaric chamber. In order to fulfill the objectives qualitative research methods were done, using a semi structured interview. The survey was conducted in two selected hospitals, in the České Budejovice Hospital, and at the University Hospital Plzeň - Lochotin. An individual, semi-structured interview was used as data collection technique, which was initially recorded on a cell phone and then transcribed into written form. At the beginning of the research interviews were conducted with nurses, who gave a comprehensive view of their job description. These conversations only complement the research. We included the brochure with basic information in our research and that was supplemented by information obtained from these interviews. The sample included patients treated in a hyperbaric chamber. They were subsequently divided into three subgroups. The first subgroup consisted of chronic patients, the second subgroup of patients undergoing hyperbaric oxygen therapy for the first time and who got the booklet with background information before their first exposure and in the third were also new patients but who were given a booklet after their first exposure. The survey was conducted in January and February 2015. Analyzed data formed four categories. Careful preparation of the patient is an essential part of treatment in a hyperbaric chamber, largely because of the specificity of this treatment, which consists mainly of placing the patient in an enclosed space, the need for a breathing mask and the inability to quickly leave the hyperbaric chamber without health risks. Therapy in a hyperbaric chamber alone is, in compliance with the indication, contraindication and dosing schedules and well-executed preparation, relatively safe and simple. From the above mentioned reasons it is necessary that the nurses in the area are sufficiently educated and that the patient who enters the hyperbaric chamber is properly prepared. Nurses educate each patient just before their first exposure, but also before every following one. There is very much information given to patients during preparation for entry into a hyperbaric chamber. The research showed that almost all the respondents had difficulty to remember all the details. Nurses tell them about hyperbaroxy essence, indications, contraindications, the exposure pattern, they select a breathing mask with them, and teach the patients to equalize the pressure in their ears, check their clothes and what they have with them. The nurses also check whether they are greased with creams, makeup and other cosmetics, and in the case of wounds if they are treated using a moist treatment instead of greasy. Before each exposure, the patient is asked about their health (a cold is a contraindication), their blood pressure is measured and the necessary nasal drops are applied. Precisely because of the considerable amount of information that patients get at their first exposure, we decided to include the booklet, which contains basic information for patients who are treated in a hyperbaric chamber for the first time, in our research.

京都大学 / 0048 / 新制・課程博士 / 博士(人間・環境学) / 甲第21845号 / 人博第874号 / 新制||人||210(附属図書館) / 2018||人博||874(吉田南総合図書館) / 京都大学大学院人間・環境学研究科共生人間学専攻 / (主査)教授 石原 昭彦, 教授 久代 恵介, 教授 神﨑 素樹 / 学位規則第4条第1項該当 / Doctor of Human and Environmental Studies / Kyoto University / DGAM

京都大学 / 新制・論文博士 / 博士(医学) / 乙第13437号 / 論医博第2236号 / 新制||医||1054(附属図書館) / (主査)教授 松田 秀一, 教授 中山 健夫, 教授 森本 尚樹 / 学位規則第4条第2項該当 / Doctor of Medical Science / Kyoto University / DFAM

京都大学 / 0048 / 新制・論文博士 / 博士(人間・環境学) / 乙第12883号 / 論人博第40号 / 新制||人||169(附属図書館) / 26||論人博||40(吉田南総合図書館) / 31601 / (主査)教授 石原 昭彦, 教授 船橋 新太郎, 教授 林 達也, 准教授 神﨑 素樹, 准教授 久代 恵介 / 学位規則第4条第2項該当 / Doctor of Human and Environmental Studies / Kyoto University / DFAM

Vitamin E and selenium play key roles in preventing in vitro lipid peroxidation and free radical damage to retinal tissues. In this research, we studied the effects of hyperbaric oxygen on retinal structure in rats fed diets deficient in vitamin E and/or selenium. We also correlated any alterations in retinal structure with previously measured alterations in electroretinograms (ERGs). Age-matched rats were fed a basal diet deficient in both vitamin E and selenium (B diet), a basal diet supplemented with vitamin E alone (B+E diet), or selenium alone (B+Se diet), or with both micronutrients (B+E+Se). Half the rats in each group were treated (+ HBO) with hyperbaric oxygen (100% O2 at 3 ATA for 1·5 per hr day, 5 days per week) and half were not (-HBO). We previously found that the rats fed the B diet for 6 weeks and treated with HBO for 4 weeks (B+HBO group) had diminished a-wave ERG amplitudes. At this time point all rats in the B group and half of the rats in the B+E+Se group were killed for the structural studies reported here. Surprisingly, we found no evidence of photoreceptor cell necrosis [i.e. a decreased thickness of the outer nuclear layer (ONL)] in retinas from rats in the B+HBO group despite the diminished amplitude of the a-wave which arises from this retinal layer. Quantitative structural analyses of retinas from rats in the B+HBO, B-HBO, B+E+Se-HBO and B+E+Se+HBO groups also failed to reveal any significant differences in the cell height of the retinal pigmented epithelium (nasal, central or temporal regions) or the number of mitochondria, phagosomas or inclusion bodies in the central retinal pigment epithelium (RPE). The inner nuclear layer (INL) thickness was, however, consistently decreased in all retinal regions for the rats in the B+HBO group. Our previous work also showed that only rats fed the B+Se diet for 17 weeks and treated with HBO for 15 weeks (B+Se+HBO group) showed diminished a-wave and b-wave ERG amplitudes. At this time point rats in the B+E+Se, B+E, and B+Se groups were killed for structural studies reported here. Only rats in the B+Se+HBO group showed a significantly decreased (about 20%) thickness of the central ONL. This evidence of photoreceptor cell necrosis correlated very well with our previous observation of diminished a- and b-wave amplitudes only in the B+Se+HBO group (at week 17). Ultrastructural studies after 17 weeks of feeding the experimental diets revealed two different types of inclusion bodies in the central RPE. On the basis of morphological appearance we have termed these inclusion bodies 'electron-dense' and 'granulated'. The central RPE of rats in the B+Se+HBO and B+Se-HBO groups showed a larger number (P < 0·001) of 'granulated' inclusion bodies and a smaller number (P < 0·001) of 'electron-dense' inclusion bodies than rats in any other diet/treatment group at this time point. In marked contrast, there were no observable 'granulated' inclusion bodies and no significant differences in the number of electron-dense inclusion bodies found in the central RPE from rats in any diet/treatment group after 6 weeks of feeding the experimental diets. Our results are discussed with respect to the potential effects of lipid peroxidation on retinal morphology and on electroretinograms.

Background: Ageing is associated with age-related disease and it has been divided into 12 hallmarks and cellular senescence is one of them. Cellular senescence increases with age and has different pathological inducing aspects in the tissue. Hyperbaric oxygen therapy is used in the clinic to treat different pathological conditions and has emerged as a possible intervention for the reduction of senescent cells. Reducing senescent cells could be a way to reduce the effects of ageing and therefore possibly in the future treat age-related diseases. Aim: This systematic literature review aims to investigate articles researching the effect of HBO exposure on senescent cells and the properties of senescent cells. Methods: PubMed database was used in this systematic literature review. Exclusion and inclusion criteria were specified using the PECO format. A search plan was created using both MeSH words and free text words. An article search was performed twice on 11 th September 2023 and 20 th November 2023. The exclusion and inclusion criteria were used to filter the search results and for the full-text review. Result: 6 articles in total were included in this study. 2 of those included articles were conducted on humans while the rest were conducted on animals or animal cells. Two studies showed that HBOT decreases the number of senescent cells, one study showed that the senescent cell markers and SASP were decreased post-HBOT, two studies showed that the properties of senescent cells were decreased post-HBOT while one study showed the contrary with an increased senescent cell properties. Conclusion: The result of this systematic literature review suggests that there is an association between HBOT and a decrease in senescent cells or its properties. More research is needed though to better understand the relationship between HBOT and the effect it imposes on senescent cells.

No description available.