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by Kin-mang Lau. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 148-167). / Chapter I --- ABSTRACT --- p.1 / Chapter II --- INTRODUCTION --- p.3 / Chapter II. 1 --- Epidemiology of prostate cancer --- p.3 / Chapter II.2 --- Anatomy of the human prostate --- p.13 / Chapter II.3 --- Pathology of prostate diseases --- p.14 / Chapter II.3.1 --- Prostatic Hyperplasia / Chapter II.3.2 --- Atypical Hyperplasia / Chapter II.3.3 --- Prostatic Carcinoma / Chapter II.4 --- Tumour Suppressor Gene - Human p53 --- p.19 / Chapter II.4.1 --- General aspects / Chapter II.4.2 --- Human p53 gene - Historical perspectives / Chapter II.4.3 --- Human p53 gene - Structure / Chapter II.4.4 --- Human p53 protein - Structure / Chapter II.4.5 --- Wild-type p53 protein - Biochemical functions / Chapter II.4.6 --- Wild-type p53 protein - Biological function / Chapter II.4.7 --- Regulation of p53 function / Chapter II.4.8 --- p53 mutations in Human cancers / Chapter II.4.9 --- Properties of mutant p53 protein / Chapter II.5 --- Human Papillomavirus (HPV) --- p.38 / Chapter II.5.1. --- Virion Structure / Chapter II.5.2 --- Classification / Chapter II.5.3. --- Papillomaviruses in Human Cancers / Chapter II.5.4. --- Relationship between p53 alteration and HPV infection / Chapter II.6 --- p53 alteration and Prostate cancers --- p.42 / Chapter II.6.1. --- Cytogenetic studies / Chapter II.6.2. --- Hybridization analysis / Chapter II.6.3. --- p53 alterations and Prostatic cell lines / Chapter III. --- OBJECTIVES OF STUDY --- p.46 / Chapter IV --- MATERIALS & METHODS --- p.47 / Chapter IV.1 --- Patients and Materials --- p.47 / Chapter IV.2 --- Histological Grading --- p.47 / Chapter IV.2.1 --- Gleason grading / Chapter IV.2.2 --- W.H.O. grading (Mostofi) / Chapter IV.3 --- Staging of Prostatic carcinoma --- p.48 / Chapter IV.4 --- Collection of Blood and Tissue samples --- p.49 / Chapter IV.5 --- Immunohistochemical studies of Prostatic lesions --- p.50 / Chapter IV.5.1 --- Antibodies used / Chapter IV.5.2 --- Methods in frozen sections / Chapter IV.5.3 --- Methods in paraffin sections / Chapter IV.5.4 --- Controls / Chapter IV.5.5 --- Immunohistochemical evaluation / Chapter IV.6 --- Extraction of DNA from tissues and blood samples --- p.52 / Chapter IV.6.1 --- Extraction of genomic DNA from blood / Chapter IV.6.2 --- Extraction of genomic DNA from tissue / Chapter IV.7 --- Hybridization analysis --- p.54 / Chapter IV.7.1 --- Preparation of Cloned Probe DNA / Chapter IV.7.2 --- Transformation of CaCl2-treated competent cell / Chapter IV.7.3 --- Cultures of Transformants / Chapter IV.7.4 --- Isolationof plasmid DNA from transformant cultures / Chapter IV.7.5 --- Purification of DNA Probe by electroelution / Chapter IV.7.6 --- Radioactive labelling of DNA Probes / Chapter IV.7.7 --- Purification of radioactive labelled DNA Probes / Chapter IV.7.8 --- Southern Blotting Technique / Chapter IV.7.9 --- Hybridization of DNA Blots with labelled DNA Probe / Chapter IV.8 --- Polymerase Chain Reaction - Single Stranded Conformational Polymorphism (PCR-SSCP) --- p.63 / Chapter IV.8.1 --- 5'-radioactive labelling of primer / Chapter IV.8.2 --- Amplification of target sequence by PCR / Chapter IV.8.3 --- Nondenaturing Polyacrylamide Gel Electrophoresis / Chapter IV.8.4 --- Direct DNA sequencing of PCR products with p53 mutation / Chapter IV.8.5 --- Controls / Chapter IV.9 --- PCR method for detection of Human Papillomavirus (HPV) --- p.71 / Chapter IV.9.1 --- PCR-Amplification / Chapter IV.9.2 --- DNA alkali Blotting Technique / Chapter IV.9.3 --- Preparation of Radioactive labelled Oligoprobes / Chapter IV.9.4 --- Hybridization of DNA Blots with radioactive labelled Oligoprobes / Chapter IV.9.5 --- Controls / Chapter IV.9.6 --- Sensitivity of HPV 18 detection by PCR / Chapter V --- RESULTS --- p.76 / Chapter V.1 --- Grading and Staging of patients with prostatic carcinoma --- p.76 / Chapter V.2 --- Immunohistochemistry in prostatic lesions --- p.80 / Chapter V.3 --- Results of hybridization analysis --- p.81 / Chapter V.4 --- PCR-SSCP findings in prostatic hyperplasia and carcinoma --- p.97 / Chapter V.5 --- PCR detection of HPV in human prostate --- p.110 / Chapter VI --- DISCUSSION --- p.125 / Chapter VII --- CONCLUSION --- p.146 / Chapter VIII --- REFERENCES --- p.148 / Chapter IX --- APPENDIX --- p.168 / Chapter X --- ACKNOWLEDGEMENT --- p.172

by Yu Hon Ming. / Thesis (M.Sc.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 70-74). / Abstract --- p.ii / Acknowledgements --- p.v / Abbreviations --- p.vi / List of tables and figures --- p.viii / Chapter Chapter I. --- Introduction / Chapter 1. --- Background --- p.1 / Chapter 2. --- Physiology of prostate --- p.3 / Chapter 2.1. --- Prostate --- p.3 / Chapter 2.2. --- Embryonic development of prostate --- p.3 / Chapter 2.3. --- Anatomy of prostate --- p.5 / Chapter 2.4. --- The role of steroids in the growth of prostate --- p.7 / Chapter 2.4.1. --- T --- p.7 / Chapter 2.4.2. --- DHT --- p.8 / Chapter 2.5. --- 5α-reductase --- p.10 / Chapter 3. --- Pathophysiology of BPH --- p.11 / Chapter 3.1. --- Anatomic progression of BPH --- p.11 / Chapter 3.2. --- Epidemiology of BPH --- p.11 / Chapter 3.3. --- Pathogenesis of BPH --- p.12 / Chapter 3.4. --- Clinical manifestations of BPH --- p.13 / Chapter 3.5. --- Diagnosis of BPH --- p.14 / Chapter 4. --- Treatment of BPH --- p.15 / Chapter 4.1. --- a-adrenergic antagonist --- p.16 / Chapter 4.2. --- DHT hypothesis --- p.16 / Chapter 4.3. --- 5α-reductase inhibitor --- p.17 / Chapter 5. --- Radioimmunoassay of DHT --- p.21 / Chapter 6. --- Objectives --- p.22 / Chapter Chapter II. --- Materials and methods --- p.23 / Chapter 1. --- Materials and methods for development of specific RIA for serum DHT --- p.23 / Chapter 1.1. --- Materials --- p.23 / Chapter 1.2. --- Methods --- p.23 / Chapter 1.2.1. --- Antiserum for DHT-RIA --- p.23 / Chapter 1.2.1.1. --- Optimal antibody titre and dilution curve for DHT-RIA --- p.24 / Chapter 1.2.1.2. --- Cross-reactivity with related steroids --- p.25 / Chapter 1.2.2. --- KMnO4 treatment of T antiserum for DHT-RIA --- p.26 / Chapter 1.2.2.1. --- Optimization of KMnO4 treatment --- p.26 / Chapter 1.2.2.2. --- Upper limit of oxidizing power of 0.5% KMnO4 --- p.27 / Chapter 1.2.2.3. --- Efficiency and background effect of 0.5% KMnO4 oxidation on DHT-RIA --- p.28 / Chapter 1.2.3. --- Characteristic of DHT-RIA --- p.29 / Chapter 1.2.3.1. --- Standard preparation --- p.29 / Chapter 1.2.3.2. --- Blank preparation --- p.30 / Chapter 1.2.3.3. --- Control preparation --- p.31 / Chapter 1.2.3.4. --- Sample preparation --- p.31 / Chapter 1.2.3.5. --- Dextran-coated charcoal --- p.31 / Chapter 1.2.3.6. --- DHT-RIA procedure --- p.32 / Chapter 1.2.3.7. --- Sensitivity test for DHT-RIA --- p.33 / Chapter 1.2.3.8. --- Linearity test for DHT-RIA --- p.33 / Chapter 1.2.3.9. --- Recovery test for DHT-RIA --- p.34 / Chapter 1.2.3.10. --- Precision test for DHT-RIA --- p.34 / Chapter 2. --- Materials and methods for establishing reference range of DHT in Chinese males and females --- p.35 / Chapter 2.1. --- Samples --- p.35 / Chapter 2.2. --- Methods --- p.35 / Chapter 2.2.1. --- DHT determination --- p.35 / Chapter 2.2.2. --- T determination --- p.36 / Chapter 2.2.3. --- Cholesterol determination --- p.37 / Chapter 3. --- Materials and methods for a small clinical trial on the usefulness of 5α-reductase inhibitor (finasteride) in the treatment of BPH --- p.38 / Chapter 3.1. --- Samples --- p.38 / Chapter 3.2. --- Methods --- p.38 / Chapter 4. --- Statistical analysis --- p.39 / Chapter Chapter III. --- Result --- p.40 / Chapter 1. --- DHT-RIA --- p.40 / Chapter 1.1. --- Antiserum for DHT-RIA / Chapter 1.1.1. --- Optimal antibody titre for DHT-RIA --- p.40 / Chapter 1.1.2. --- Cross reactivity with related steroids --- p.42 / Chapter 1.2. --- KMnO4 treatment of T antiserum for DHT-RIA --- p.43 / Chapter 1.2.1. --- Optimization of KMnO4 treatment --- p.43 / Chapter 1.2.2. --- Upper limit of oxidizing power of 0.5% KMnO4 --- p.44 / Chapter 1.2.3. --- Efficiency and background effect of 0.5% KMnO4 treatment on DHT-RIA --- p.45 / Chapter 1.3. --- Characterization of the DHT-RIA --- p.47 / Chapter 2. --- Reference range for DHT in Chinese males and females --- p.51 / Chapter 2.1. --- Reference range of DHT in three different study groups --- p.51 / Chapter 2.2. --- Ratio of DHT/T in Chinese males and Caucasian males --- p.52 / Chapter 2.3. --- Correlation studies --- p.53 / Chapter 3. --- Results of small clinical trial --- p.54 / Chapter 3.1. --- Mean serum analyte changes during the course --- p.54 / Chapter 3.2. --- Changes in analyte concentrations during the course --- p.56 / Chapter 3.3. --- Difference in mean serum analytes between BPH males predose level and normal males with and without age matching --- p.57 / Chapter 3.4. --- "The response, percentage change of DHT and DHT/T ratio of individual patient during the course of study" --- p.58 / Chapter Chapter IV. --- Discussion --- p.59 / Chapter 1. --- Radioimmunoassay for DHT --- p.59 / Chapter 2 . --- Establishment of DHT reference range in Chinese males and females --- p.62 / Chapter 3. --- The usefulness of 5a reductase inhibitor in the treatment of BPH --- p.65 / Chapter Chapter V. --- Conclusion --- p.69 / References --- p.70

Includes bibliographical references (leaves 281-328) / iii, 328 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / The experiments reported are investigations of the pituitary of rats stunted by undernutrition imposed between birth and 3 weeks of age. / Thesis (Ph.D.)--University of Adelaide, Dept. of Animal Physiology, 1971

Includes bibliographical references (leaves 281-328) The experiments reported are investigations of the pituitary of rats stunted by undernutrition imposed between birth and 3 weeks of age.

Thesis (Ph. D.)--University of North Carolina at Greensboro, 2006. / Advisor: Ron Morrison; submitted to the School of Human Environmental Sciences. Includes bibliographical references.

This research investigated the changes in body adipocyte size and density as well as parameters relating to adipogenesis in growing cobia, Rachycentron canadum. The cobia from a same batch were sampled 5 , 7 , 9 , 13 , 17 , 21 , 26 and 31 weeks after hatching, Liver activities of fatty acid synthase, (FAS), mailc enzyme (ME), and glucose-6-phosphate dehydrogenase (G6PDH) as well as serum total triacylglycerol (TG), total cholesterol (TC), phospholipid (PL), non-esterified fatty acid (NEFA) were assayed. Adipocyte abundance and size spectrum in liver, ventral muscle and dorsal muscle were determined. The cobia attained an average weight of 13.6 ¡Ó 0.5 g at week 5, and were cultured in nursery outdoor ponds until an average weight 87.5 ¡Ó 3.8 g when moved to open ocean cages. They grew to an average weight 1690.8 ¡Ó 106.6 g at week 31. The crude lipid content in liver, ventral and dorsal muscle increased significantly with fish age, and were 36.1 ¡Ó 3.2 g, 14.8 ¡Ó 0.2g, 4.6 ¡Ó 0.0 g at week 31. No significant change was found in hepatic FAS activity. Hepatic ME activity increased significantly after the fish were stocked in the cages. Whilr hepatic ME activity reduced with age, TG and PL were both increased significantly with age. While adipocyte diameter in liver showed a decreasing trend as the fish grew its abundance increased significantly at week 13 and maintained between 3070¡ã3356 x 104 cell/g tissue during week 17-31. Similarly, adipocyte abundance in ventral muscle increased significantly at week 13, and maintained between 255¡ã269 x 104 cell/g tissue afterwards. There was no significant change in size of ventral muscle adipocyte as fish grew. These results indicate that the adipocyte hyperplasia occurred during week 7- 13, when body weight was 100- 400 g. Adipocyte hypertrophy was observed when the fish reached week 31, there was a sign of adipocyte hypertrophy in liver when the fish were at the last phase of nursery outdoor ponds before being moved to the offshore cages.

Thesis--M. Med. Sc., University of Hong Kong, 1982.

published_or_final_version / Nursing Studies / Master / Master of Nursing in Advanced Practice