SUBMITTED IN PARTIAL FULFILMENT OF THE REQUIREMENT FOR THE AWARD OF THE DEGREE OF MASTER OF FAMILY MEDICINE FACULTY OF HEALTH SCIENCES, UNIVERSITY OF THE WITWATERSRAND. JOHANNESBURG 2014 / INTRODUCTION: The Under-5 Clinic in Johan Heynes Community Health Centre is always packed and has high immunization rates (104%). Despite this, during clinical consultation, the family physician researcher suspected that mothers were poorly informed about childhood immunization, which immunization the child had received and symptoms of common side effects. OBJECTIVES: This study assessed mothers understanding of indications, benefits, adverse effects of childhood immunization, how to catch-up on missed vaccinations, and how they obtained information about childhood immunization. METHODS: A cross sectional descriptive study was undertaken of all mothers attending immunization services at the clinic. Systematic sampling of 302 mothers using face to face interviews was done. The instrument questions were obtained from 2 similar validated studies, adapted to suit the setting, and piloted. Data was collected from 15th November to 15th December 2012. Data was analyzed using Epi-info. RESULTS: Ninety seven percent of mothers brought their children for immunization because they feared their children could develop illnesses. Seven percent of mothers knew what vaccines their children would receive on the day of immunization and what diseases these vaccines prevent. Nearly all mothers were given information on when to immunize their children. Fourteen percent where given information on why they need to immunize their children. CONCLUSION: Most mothers knew that immunization prevented certain illnesses, but did not know which illnesses were being immunized against. Further, most mothers didn’t know about the common side effects of childhood immunization. However, most mothers were well informed about the timing of immunization.

Thesis (M.Sc. in Microbiology) -- Mahidol University, 1976.

BACKGROUND: The widespread use of vaccines led to significant decline in multiple potentially fatal infectious diseases. Recently, there has been an increase in vaccine hesitancy. Measles and pertussis outbreaks throughout the United States have put a spotlight on this urgent healthcare issue. Motivational interviewing is a counseling tactic that is gaining popularity and is being studied for its efficacy in preventative medicine and psychological disorders. It aims to inspire people to make behavioral changes through collaborative relationships with their provider by understanding how current actions do not translate into their health goals. LITERATURE REVIEW FINDINGS: Vaccine hesitancy is growing. Communities with decreased immunization rates are associated with a higher risk of disease outbreak. Increasing rates of undervaccinated children are likely due to increases in non-medical exemptions. Many parents, regardless of their vaccine hesitancy status, are concerned about vaccine safety. Vaccine hesitant parents refuse vaccines due to philosophical and religious beliefs, conspiracy theories, and safety concerns. Parents feel that providers do not adequately address their concern. Providers report not having the training to discredit parental concerns. The majority of parents describe their child’s pediatrician as their most trusted source of vaccine information. Parents who receive vaccine information from a provider are more likely to comply with the recommended childhood vaccine schedule. The most efficient way to discuss vaccines with parents has yet to be determined. PROPOSED PROJECT: This is a proposed QI research project for the Pediatric Clinic at Boston Medical Center. Providers would be trained in motivational interviewing during several sessions that included lectures and small group practice sessions with systematic feedback. During the intervention, parents who refuse vaccines for their child, aged 0-6 years old, will receive motivational interviewing from the provider. The proportion of the vaccine hesitant parents who accept the offered vaccine after will be analyzed. The pre and post intervention vaccination rates for the entire clinic will also be assessed. Data collection will be preformed through retrospective chart review. The project aims to increase provider confidence on vaccine counseling, educate providers on reasons for hesitancy, and improve compliance with the CDC recommended vaccine schedule. CONCLUSION: While most Americans continue to vaccinate their children according to the CDC’s recommended schedule, constant vigilance is required to maintain high immunization rates to protect our communities. Motivational interviewing is goal-oriented to alter a specific behavior and would allow providers to engage in an open, persuasive dialogue about parental vaccine concerns.

Thesis (MCur)--Stellenbosch University, 2011. / ENGLISH ABSTRACT: Parents and/or caregivers all over the world are expected to comply with existing childhood immunisation programmes in order to prevent outbreaks of preventable childhood diseases. The most important justification for this study was the 2010 measles outbreak in the Western Cape province of South Africa. This outbreak correlated with the study conclusions of Corrigall, Coetzee and Cameron (2008:41) as they found the immunisation coverage in the Western Cape to be insufficient to prevent outbreaks of preventable childhood diseases. From the literature, it seemed that attitudes and factors that influence parent and/or caregiver compliance with the routine childhood immunisation schedule in the Witzenberg sub-district of the Western Cape persist. In doing this study, the researcher’s purpose was to discover what those factors were. The aim was to determine, understand and describe the attitudes and factors influencing parent and/or caregiver compliance with the routine childhood immunisation schedule, and the nature thereof. The set objectives were to gain knowledge of and insight into the factors influencing participants’ adherence with routine childhood immunisation; and participants’ feelings, attitudes, and experiences surrounding immunisation within the context of their health care environment. A quantitative research approach, with a smaller qualitative component, was selected for this study which had an exploratory descriptive design. The population targeted for data collection included parents and/or caregivers of children aged 0 to 60 months in the Witzenberg sub-district (N=8374), as well as in community health centres (CHCs) that provided immunisation and/or other primary health care services in the mentioned subdistrict (N=16). The non-probability convenience sampling of parents and/or caregivers consisted of 376 participants (n=376), while 8 CHCs (n=8) were selected through systematic sampling. The measuring instrument used as the data collection technique for this study was a selfadministered questionnaire. A pilot study was conducted to pre-test the questionnaire, and its reliability and validity were further ensured by submitting it for review to research experts in methodology and nursing. A combination of quantitative and qualitative methods was used to analyse the study data. MS Excel was used to capture the quantitative data after which it was analysed using descriptive statistics by means of STATISTICA version 9-software. Tesch’s approach to qualitative data analysis was used as a guideline with the purpose of identifying and categorising the essential qualitative data and grouping it together in one descriptive framework. The main findings were that parents and/or caregivers involved in this study were positive about immunisation and their experiences within the health service environment. They also felt that health workers were playing an important role in their decision-making process. However, their knowledge regarding the purpose of and contra-indications for immunisation were insufficient, and most parents reported that their children experienced side effects after immunisation. Key recommendations on conclusion of the study include clear, accurate and specific information to parents about the purpose of immunisation and its contra-indications and side effects. Vaccinators and managers should be informed about the persistent problem with mild vaccination side effects and refresher courses should be provided with regard to infection control, administration techniques and the reporting of adverse effects. / AFRIKAANSE OPSOMMING: Daar word van ouers en/of versorgers oor die wêreld verwag om aan bestaande kinderimmunisasieprogramme te voldoen ter voorkoming van vaksien-voorkombare siektes. Die belangrikste regverdiging vir die studie was die masel-uitbreking in die Wes-Kaapprovinsie van Suid-Afrika in 2010. Die uitbraak het die studiebevindinge ondersteun van Corrigall, Coetzee en Cameron (2008:41), wat bevind het dat immunisasiedekking in die Wes-Kaap onvoldoende was om ʼn uitbreking van voorkombare kindersiektes te verhoed. Volgens die literatuur het dit voorgekom asof die gesindhede en faktore wat ouers en/of versorgers se houding jeens die roetine- kinder-immunisasieskedule in die Witzenberg sub-distrik van die Wes-Kaap beïnvloed, voortduur. Die navorser het met hierdie studie ten doel gehad om die faktore te ontdek, ten einde die gesindhede en faktore wat ‘n invloed uitoefen te bepaal, te verstaan en te omskryf. Die doelwitte was om kennis in te win oor en insig te verkry in die faktore wat ouers en/of versorgers se aanhanklikheid met routine kinder immunisering beinbloed; en in ouers en/of versorgers se gevoelens, houdings en ondervindings ten opsigte van immunisasie in hulle gesondheidsorg-omgewing. ‘n Kwantitatiewe navorsingsbenadering met ‘n kleiner kwalitatiewe komponent is geselekteer vir die studie wat ‘n ondersoekend-beskrywende navorsingsontwerp gehad het. Die populasie wat geteiken is vir data-insameling was ouers en/of versorgers van kinders van 0 tot 60 maande in die Witzenberg sub-distrik (N=8374), asook gemeenskapsgesondheidsentrums wat immunisasie en/of ander primêre gesondheidsorgdienste in die genoemde sub-distrik aanbied (N=16). Die nie-waarskynlike gerieflikheidsteekproef van ouers en/of versorgers het uit 376 deelnemers (n=376) bestaan, terwyl 8 (n=8) gemeenskapsgesondheidsentrums deur middel van sistematiese steekproefbepaling geselekteer is. Die meetinstrument vir data-insameling in die studie was self-geadministreerde vraelyste. ‘n Loodsstudie is uitgevoer as vooraf-toetsing van die vraelys en die geldigheid en betroubaarheid daarvan is verder verseker deur die vraelys aan navorsingskenners in navorsingsmetodologie en verpleging te onderwerp. ‘n Kombinasie van kwantitatiewe en kwalitatiewe metodes is gebruik vir die analisering van die studie-data. Die kwantitatiewe data is op MS Excel ingevoer waarna beskrywende statistieke deur middel van Statistica weergawe 9-sagteware gebruik is om dit te analiseer. Tesch se benadering tot kwalitatiewe data-analise is as riglyn gebruik met die doel om belangrike data te identifiseer, te kategoriseer en in ‘n beskrywende raamwerk te groepeer. In die studie is daar hoofsaaklik bevind dat ouers en/of versorgers positief is oor immunisasie en hul ondervinding binne die gesondheidsorgomgewing, en dat gesondheidswerkers ʼn belangrike rol in hul besluitnemingsproses speel. Hul kennis aangaande die doel van en kontra-indikasies vir immunisasie was egter onvoldoende en die meeste ouers en/of versorgers het gerapporteer dat hul kinders ná immunisasie probleme met newe-effekte ondervind het. Die hoofaanbevelings wat uit die studie voortgespruit het, was dat duidelike, akkurate en spesifieke inligting aan ouers en/of versorgers verskaf moet word aangaande die doel van immunisasie en die kontra-indikasies daarvoor. Immuniseerders en bestuurders moet ook ingelig word oor die voortdurende voorkoms van newe-effekte sodat opknappingskursusse oor infeksiebeheer, korrekte toedieningstegnieke en die rapportering van newe-effekte aangebied kan word.

Thesis (D.P.H.)--University of Michigan.

Thesis (D.P.H.)--University of Michigan.

Os modelos experimentais reproduzem doenças que acometem seres humanos, sendo de extrema importância porque possibilitam o estudo da patogênese e abordagem terapêutica dessas enfermidades. Nesse grupo inclui-se o modelo experimental de esclerodermia induzida pela imunização de coelhos com colágeno V humano provocando alterações histológicas (pele, pulmão e rim) similares àquelas observadas em humanos. As doenças auto-imunes têm sua etiologia desconhecida e são particularmente caracterizadas pela presença de auto-anticorpos no soro. Nesse aspecto, 90 a 95% dos pacientes com esclerodermia apresentam algum auto-anticorpo contra antígenos intracelulares (proteínas nucleolares RNA polimerase I, II e III, Scl-70, centriolares ou golginas) ou da matriz extracelular (colágeno). O presente estudo tem como objetivo avaliar a resposta imunológica nos animais do modelo experimental de esclerodermia. Para tanto, o soro dos animais foram testados quanto à presença de auto-anticorpos e de outros fatores imunológicos séricos que indicassem um processo imunológico ativo paralelo às lesões teciduais em desenvolvimento e incluiu: pesquisa de anticorpos anticolágenos V, III e I, imunocomplexos circulantes, fator reumatóide, níveis de complemento, fatores antinucleares (FAN) por imunofluorescência indireta em células HEp-2 e caracterização dos antígenos alvos por immunoblot. A análise da resposta imune revelou que as alterações histológicas do pulmão, rim e pele se desenvolviam com o aumento dos níveis séricos de anticorpos anti-colágeno V. Além disso, todos os animais imunizados com Col V apresentavam anticorpos para outros tipos de colágeno. Esses animais desenvolveram uma marcante resposta imunológica a antígenos intracelulares com 100% de positividade para o FAN e presença de imunocomplexos nos soros obtidos 30, 75 e 120 dias pós-imunização quando comparados a dois grupos controles (albumina e adjuvante completo de Freud). Todos os animais do grupo Col V apresentaram na IFI padrão citoplasmático Golgi símile e, em 10% deles, reatividade adicional aos centríolos. Ambos auto-anticorpos são raros, mas já descritos em pacientes com esclerodermia. A pré-absorção dos soros desses animais com Col V não interferiu no resultado do FAN. A caracterização dos antígenos alvos mostrou uma reatividade uniforme a proteínas de alto peso molecular de células epiteliais humanas (maior ou igual 175 kDa) que progredia com o tempo de imunização. Eluatos ácidos contendo os anticorpos anti-fração 175 kDa reproduziram o padrão de IFI igual ao soro original. As análises demonstram que a imunização com Col V humano em coelhos resulta numa resposta imunológica exuberante e que se caracteriza pela presença de auto-anticorpos a componentes intracelulares. / Experimental models for human diseases are of utmost importance, since they allow the study of their pathogenesis and therapeutic approach. In this group we can include the experimental model of collagen V-induced scleroderma, in rabbits, with histological alterations (skin, lung and kidney) similar to those observed in humans. Auto-immune diseases have an unknown etiology, and are characterized by the presence of auto-antibodies in serum. In this aspect, 90%-95% of the patients with scleroderma present some kind of auto-antibody against intracellular antigens (RNA polymerase I, II and III nucleoproteins, Scl-70, centriolar or golgins) or to components of the extracellular matrix (collagen). This study aims to assess the immune response of the animal subjects in a scleroderma experimental model. The animals sera were tested for auto-antibodies and other serum immunological factors that would point to an active immunological process, parallel to the developing tissue lesions, including anti-collagen V, III and I antibodies, circulating immune complexes, rheumatoid factor, complement levels, antinuclear antibodies (ANA) by indirect immunofluorescence in HEp-2 (IFI) cells, and characterization of target antigens with an immunoblot. The analysis of the immune response in the studied animals revealed that histological alterations in lungs, kidneys and skin developed with an increase of the serum levels of anti-collagen V antibodies. Furthermore, all the Col Vimmunized animals presented antibodies against other types of collagen as well. These animals also developed a strong immune response against intracellular antigens, being 100% positive for ANA, showing also immune complexes in sera obtained 30, 75 and 120 days after immunization with Col V, when compared to the control groups (albumin and Freunds complete adjuvant). All the animals from the Col V group showed a cytoplasmic pattern at the IFI, Golgi simile and, in 10% of the cases, additional reactivity to the centrioles. Both auto-antibodies are rare, yet were already described in patients with scleroderma. The pre-absorption of these animals sera with Col V did not interfere with the ANA reactivity. The characterization of the target antigens showed a uniform reactivity to high molecular weight proteins of human epithelial cells (> or = 175 kDa), which progressed with the immunization time. Acid eluats containing antibodies against the 175 kDa fraction reproduced the same IFI reactivity pattern of the original serum. The results demonstrate that immunization of rabbits with human Col V results in an exuberant immune response, characterized by the presence of autoantibodies against intracellular components.

Os modelos experimentais reproduzem doenças que acometem seres humanos, sendo de extrema importância porque possibilitam o estudo da patogênese e abordagem terapêutica dessas enfermidades. Nesse grupo inclui-se o modelo experimental de esclerodermia induzida pela imunização de coelhos com colágeno V humano provocando alterações histológicas (pele, pulmão e rim) similares àquelas observadas em humanos. As doenças auto-imunes têm sua etiologia desconhecida e são particularmente caracterizadas pela presença de auto-anticorpos no soro. Nesse aspecto, 90 a 95% dos pacientes com esclerodermia apresentam algum auto-anticorpo contra antígenos intracelulares (proteínas nucleolares RNA polimerase I, II e III, Scl-70, centriolares ou golginas) ou da matriz extracelular (colágeno). O presente estudo tem como objetivo avaliar a resposta imunológica nos animais do modelo experimental de esclerodermia. Para tanto, o soro dos animais foram testados quanto à presença de auto-anticorpos e de outros fatores imunológicos séricos que indicassem um processo imunológico ativo paralelo às lesões teciduais em desenvolvimento e incluiu: pesquisa de anticorpos anticolágenos V, III e I, imunocomplexos circulantes, fator reumatóide, níveis de complemento, fatores antinucleares (FAN) por imunofluorescência indireta em células HEp-2 e caracterização dos antígenos alvos por immunoblot. A análise da resposta imune revelou que as alterações histológicas do pulmão, rim e pele se desenvolviam com o aumento dos níveis séricos de anticorpos anti-colágeno V. Além disso, todos os animais imunizados com Col V apresentavam anticorpos para outros tipos de colágeno. Esses animais desenvolveram uma marcante resposta imunológica a antígenos intracelulares com 100% de positividade para o FAN e presença de imunocomplexos nos soros obtidos 30, 75 e 120 dias pós-imunização quando comparados a dois grupos controles (albumina e adjuvante completo de Freud). Todos os animais do grupo Col V apresentaram na IFI padrão citoplasmático Golgi símile e, em 10% deles, reatividade adicional aos centríolos. Ambos auto-anticorpos são raros, mas já descritos em pacientes com esclerodermia. A pré-absorção dos soros desses animais com Col V não interferiu no resultado do FAN. A caracterização dos antígenos alvos mostrou uma reatividade uniforme a proteínas de alto peso molecular de células epiteliais humanas (maior ou igual 175 kDa) que progredia com o tempo de imunização. Eluatos ácidos contendo os anticorpos anti-fração 175 kDa reproduziram o padrão de IFI igual ao soro original. As análises demonstram que a imunização com Col V humano em coelhos resulta numa resposta imunológica exuberante e que se caracteriza pela presença de auto-anticorpos a componentes intracelulares. / Experimental models for human diseases are of utmost importance, since they allow the study of their pathogenesis and therapeutic approach. In this group we can include the experimental model of collagen V-induced scleroderma, in rabbits, with histological alterations (skin, lung and kidney) similar to those observed in humans. Auto-immune diseases have an unknown etiology, and are characterized by the presence of auto-antibodies in serum. In this aspect, 90%-95% of the patients with scleroderma present some kind of auto-antibody against intracellular antigens (RNA polymerase I, II and III nucleoproteins, Scl-70, centriolar or golgins) or to components of the extracellular matrix (collagen). This study aims to assess the immune response of the animal subjects in a scleroderma experimental model. The animals sera were tested for auto-antibodies and other serum immunological factors that would point to an active immunological process, parallel to the developing tissue lesions, including anti-collagen V, III and I antibodies, circulating immune complexes, rheumatoid factor, complement levels, antinuclear antibodies (ANA) by indirect immunofluorescence in HEp-2 (IFI) cells, and characterization of target antigens with an immunoblot. The analysis of the immune response in the studied animals revealed that histological alterations in lungs, kidneys and skin developed with an increase of the serum levels of anti-collagen V antibodies. Furthermore, all the Col Vimmunized animals presented antibodies against other types of collagen as well. These animals also developed a strong immune response against intracellular antigens, being 100% positive for ANA, showing also immune complexes in sera obtained 30, 75 and 120 days after immunization with Col V, when compared to the control groups (albumin and Freunds complete adjuvant). All the animals from the Col V group showed a cytoplasmic pattern at the IFI, Golgi simile and, in 10% of the cases, additional reactivity to the centrioles. Both auto-antibodies are rare, yet were already described in patients with scleroderma. The pre-absorption of these animals sera with Col V did not interfere with the ANA reactivity. The characterization of the target antigens showed a uniform reactivity to high molecular weight proteins of human epithelial cells (> or = 175 kDa), which progressed with the immunization time. Acid eluats containing antibodies against the 175 kDa fraction reproduced the same IFI reactivity pattern of the original serum. The results demonstrate that immunization of rabbits with human Col V results in an exuberant immune response, characterized by the presence of autoantibodies against intracellular components.