Estrogens produced by livestock can be released into soils when their manure is spread onto agricultural land. This is the first study to determine the sorption of a range of estrogens in a wide range of soils at the regional scale, including the sorption of the phytoestrogen equol which had never been previously studied. Sorption increased in the order of 17β-estradiol=estriol <estrone<equol in surface soils collected from 41 agricultural fields in Alberta and was significantly positively correlated with soil organic carbon content (SOC) for all estrogens. 17β-estradiol was further investigated and its mineralization in non-amended and manure-amended soils never exceeded 30% at 90 days, which suggest that even under optimum environmental conditions for mineralization, 17β-estradiol or its metabolites estrone and/or estriol appear to have a relatively long persistence in Alberta soils. Maximum 17β-estradiol mineralization was significantly positively correlated with sorption and hence increased in soils with greater SOC such as those used in this study with a long-term history of solid beef manure applications. Two ELISAs were developed using rabbit polyclonal antibodies for future field experiments and environmental monitoring. Of these, a developed 17β-estradiol+estrone+estriol ELISA could detect estriol in water from an edge of field experiment at concentrations as low as 1 ng mL-1.

No description available.

Contemporary proximity extension assays (PEAs) are used for qualitative proteinquantifications in serological samples, with possibilities for scaling assays in multiplex. Medical research can however benefit from robust immunoassays functional for assessingprotein levels in other types of biospecimens. Formalin-fixed paraffin embedded (FFPE)tissues have long been used for morphological studies. The proteome encapsulated byextensive cross-linking from formalin fixation has however impeded the development ofproteomic analysis from the vast biorepositories FFPE-tissues constitute. In this study, Ipresent a proof of concept for assessing FFPE-samples in multiplex format through PEA.Furthermore, a homogenization and protein extraction protocol for assessing fresh-frozentissue with PEA is presented, together with a novel sample buffer for which remarkable risesin protein detection can be seen in several protein assays. Together, these findings extend theapplication area of PEA to tissues together with improved quantification characteristics.

Estrogens produced by livestock can be released into soils when their manure is spread onto agricultural land. This is the first study to determine the sorption of a range of estrogens in a wide range of soils at the regional scale, including the sorption of the phytoestrogen equol which had never been previously studied. Sorption increased in the order of 17β-estradiol=estriol <estrone<equol in surface soils collected from 41 agricultural fields in Alberta and was significantly positively correlated with soil organic carbon content (SOC) for all estrogens. 17β-estradiol was further investigated and its mineralization in non-amended and manure-amended soils never exceeded 30% at 90 days, which suggest that even under optimum environmental conditions for mineralization, 17β-estradiol or its metabolites estrone and/or estriol appear to have a relatively long persistence in Alberta soils. Maximum 17β-estradiol mineralization was significantly positively correlated with sorption and hence increased in soils with greater SOC such as those used in this study with a long-term history of solid beef manure applications. Two ELISAs were developed using rabbit polyclonal antibodies for future field experiments and environmental monitoring. Of these, a developed 17β-estradiol+estrone+estriol ELISA could detect estriol in water from an edge of field experiment at concentrations as low as 1 ng mL-1.

No description available.

Diss. (sammanfattning) Uppsala : Univ., 2002. / Härtill 4 uppsatser.

Zugl.: Bonn, Universiẗat, Diss., 2007.

Zugl.: München, Techn. Univ., Diss., 2007

Thesis (M. Phil.)--University of Hong Kong, 1999. / Includes bibliographical references (leaves 122-135).

Thesis (M.S.)--Oregon State University, 2010. / Printout. Includes bibliographical references (leaves 155-164). Also available on the World Wide Web.