Thesis (Ph. D.)--University of Wisconsin--Madison, 1970. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 122-135).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1955. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographies: leaves 30-31, 83-85.

Thesis (M.S.)--University of Wisconsin--Madison, 1949. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves i-iii).

Thesis (M.Sc.)(Biochemistry)--University of Pretoria, 2004. / Summaries in English and Afrikaans. Includes bibliographic references.

Thesis (Ph. D.)--University of Texas at Austin, 2004. / Supervisors: Christian P. Whitman and Brent L. Iverson. Vita. Includes bibliographical references.

Thesis (Ph. D.)--University of Chicago, Dept. of Molecular Genetics and Cell Biology, March 2001. / Includes bibliographical references. Also available on the Internet.

Master of Science / Department of Animal Sciences and Industry / Jim L. Nelssen / Two experiments evaluated the effect of colostrum and energy supplementation on the performance and immune response of baby piglets. In Exp. 1, 301 newborn pigs (Line 600 × 241; DNA, 1.48 kg) were used in a 21-d study. Pigs were weighed and allotted to one of three treatments at 6-h of age in a randomized complete block design with 23 replications (litters) per treatment. Piglets were blocked by weight and randomly assigned a treatment. Runt piglets (birthweight < 0.8 kg) were tested in experiment 1 and 2. Dietary treatments were a control with no dietary supplementation, an energy supplement (1.5 ml containing glucose, dried milk, medium chain triglycerides, and tea extract), and bovine colostrum (30 ml). The supplements were given as an oral gavage. A single treatment was administered at 6-h after birth. At 30-h of age approximately 1ml of blood was obtained for an immunocrit assay of serum. The glucose based energy supplement (milk protein, medium chain triglycerides) had no (P > 0.05) effect on weight or ADG at any of the weigh periods (30-h, d 5, d 7, d 14, and weaning), immunocrit ratio, or survival rate. The bovine colostrum treatment had a negative (P < 0.05) effect on weight at 24-h, d 5, and d 7, immunocrit ratio, and survival rate. There was no (P > 0.05) effect of treatment on weight at weaning. . In Exp. 2, 364 newborn pigs (Line 600 × 241; DNA, 1.48 kg) were used in a 21-d study. Pigs were weighed and allotted to one of three treatments in a randomized complete block design with 25 replications (litters) per treatment. Dietary treatments were a control with no dietary supplementation, an energy supplement (1.5 ml, glucose based, containing milk protein, medium chain triglycerides, and tea extract), and bovine colostrum (10 ml). The supplements were given as an oral gavage. A single treatment was administered at 6-h after birth. At 30-h of age blood was collected for analysis of serum immunocrit. Body weights, ADG during the duration of the trial, immunocrit ratio, and survival rates were similar (P > 0.05) for the treatment groups. In both experiment 1 and 2 there were no treatment by weight group interaction. In summary, under the conditions of these experiments supplementation of 30 ml of bovine colostrum had a negative effect (P < 0.05) on immunocrit ratio and survival rate (P > 0.05), of the treatments affected on weaning weights when compared to the control.

nÃo hà / Lectins are a group of proteins present in various living organisms, particularly in plants and especially in Leguminosae seeds. Among this family, a species that stands out for having large amount of lectin in the seed is the Canavalia brasiliensis. One approach for the investigation of lectins on biological systems is the detection there of by techniques involving antibody. Such techniques may include from classical immunochemical assays (immunodiffusion, immunoelectrophoresis and rocketimunoeletroforese) to ELISA experiments and advanced microscopy techniques. In this sense, antibodies become biotechnological tools invaluable since they are capable of specifically recognizing epitopes of the target molecules. Immunoglobulins (Igs) are proteins present in high concentration in the blood plasma of mammals. They are vectors of humoral immunity, with the primary function to join the foreign antigens to the individual so as to neutralize them. In the case of poultry the main immunoglobulin class (IgYs) can be found not only in blood, but also in egg yolk. This fact enables fast and efficient purification of these molecules facilitating its use as biotechnological inputs. Thus, this study aimed to produce antibodies against the lectin extracted from Canavalia brasiliensis seed (ConBr) (Fabaceae) in laying hens, implementing production methodology of new biotechnological inputs for the study of lectins. For this laying hens were immunized with 200 or 400&#956;g of lectin Canavalia brasiliensis (ConBr) for 15 weeks at 10-day intervals. Collected eggs from vaccinated birds had their isolated and precipitated yolk in 0-30% fraction with ammonium sulfate for partial purificaÃÃoo of IgYs. Confirmation of the presence of antibodies was made by immunodiffusion assays agarose gel. The fraction showed 0-30%, from the first week after the immunization, capable of recognizing ConBr IgYs. This profile appeared in all groups over the 15-week experiment. The production of specific IgYs against the lectin from Canavalia brasiliensis (ConBr) proved feasible and remained throughout the experiment, possibly in reasonable quantities. In this sense, IgYs produced in this work provide a powerful biotechnological tool to be made available for future studies. / As lectinas constituem um grupo de proteÃnas presentes em diversos organismos vivos, sobretudo em vegetais e especialmente em sementes de Leguminosae. Dentre essa famÃlia, uma espÃcie que se destaca por possuir grande quantidade de lectina na semente à a Canavalia brasiliensis. Uma das possÃveis abordagens para a investigaÃÃo de lectinas em sistemas biolÃgicos à a detecÃÃo das mesmas atravÃs de tÃcnicas que envolvam anticorpos. Tais tÃcnicas podem incluir desde ensaios clÃssicos de imunoquÃmica (imunodifusÃo, imunoeletroforese e rocketimunoeletroforese), atà experimentos de ELISA e tÃcnicas avanÃadas de microscopia. Neste sentido, os anticopos tornam-se ferramentas biotecnolÃgicas de grande valia uma vez que sÃo capazes de reconhecer especificamente epÃtopos das molÃculas alvo. As imunoglobulinas (Igs) sa&#771;o protei&#769;nas presentes em grande concentrac&#807;a&#771;o no plasma sanguÃneo de mamÃferos. Sa&#771;o os vetores da imunidade humoral, tendo como func&#807;a&#771;o principal unir-se aos anti&#769;genos estranhos ao indivÃduo, de modo a neutraliza&#769;-los. No caso das aves a principal classe de imunoglobulinas (IgYs) pode ser encontrada nÃo somente no sangue mas tambÃm nas gemas dos ovos. Esse fato permite purificaÃÃo rÃpida e eficiente dessas molÃculas facilitando seu uso como insumos biotecnolÃgicos. Dessa forma, este trabalho teve por objetivo produzir anticorpos contra a lectina extraÃda da semente de Canavalia brasiliensis (ConBr) (Leguminosae) em galinhas poedeiras, implementando metodologia de produÃÃo de novos insumos biotecnolÃgicos para o estudo de lectinas. Para tanto galinhas poedeiras foram imunizadas com 200 ou 400&#956;g da lectina de Canavalia brasiliensis (ConBr) por 15 semanas em intervalos de 10 dias. Ovos coletados das aves imunizadas tiveram suas gemas isoladas e precipitadas na fraÃÃo 0-30% com sulfato de amÃnio para a purificaÃÃo parcial das IgYs. A confirmaÃÃo da presenÃa dos anticorpos foi feita por ensaios de imunodifusÃo em gel de agarose. A fraÃÃo 0-30% apresentou, a partir da primeira semana apÃs a imunizaÃÃo, as IgYs capazes de reconhecer ConBr. Esse perfil se apresentou em todos os grupos ao longo das 15 semanas de experimento. A produÃÃo das IgYs especÃficas contra a lectina de Canavalia brasiliensis (ConBr) se mostrou viÃvel e permaneceu durante todo experimento, possivelmente em quantidades razoÃveis. Neste sentido, as IgYs produzidas nesse trabalho constituem uma potente ferramenta biotecnolÃgica a ser disponibilizada para estudos futuros.

Abstract In the search for more reliable blood markers for excessive alcohol consumption, considerable effort has been devoted to measurements of carbohydrate-deficient transferrin (CDT), which increases in body fluids as a result of prolonged alcohol intake. In the present work, three CDT methods, CDTect (Pharmacia &amp; Upjohn), %CDT radioimmunoassay (%CDT RIA) by Axis (Oslo, Norway), and Axis %CDT turbidimetric immunoassay (%CDT TIA) were examined for their diagnostic performance in cases of alcohol abuse with or without liver disease. The diagnostic performance of CDT as a marker of alcohol abuse correlates positively with alcohol consumption. As compared with g-glutamyltransferase (GGT) and mean corpuscular volume of erythrocytes (MCV), which are conventionally used as laboratory markers of excessive ethanol consumption, CDT (CDTect) has the highest sensitivity (64%) at the specificity level of 100% in heavy drinkers consuming &gt;100 g ethanol/day, but its sensitivity decreases to 34% in cases with an alcohol intake of &lt;100 g/day, which hampers the use of CDT as a community screening method. Patients with alcoholic liver disease (ALD) have significantly higher CDT values than alcoholics with non-liver pathology. However, CDT is primarily increased in cases with an early stage of ALD, so that there is a weak negative correlation between CDT and disease severity, which may prove to be of diagnostic value. Especially in men, CDTect seems to achieve greater sensitivity than %CDT RIA or %CDT TIA for detecting recent alcohol abuse among heavy drinkers, but it does have a significant correlation with serum transferrin, especially in individuals reporting social drinking or no alcohol intake. This should be considered when interpreting the assay results in patients with increased serum transferrin. %CDT methods achieve greater specificity than CDTect when analyzing samples from patients with high serum transferrin concentrations. Acetaldehyde-protein adducts are formed in the body after excessive ethanol intake, and their formation triggers antibody production, which may contribute to some forms of tissue damage seen in alcohol abusers. To obtain more information on the association between serum antibodies against acetaldehyde adducts, ALD and alcohol consumption, assays for antibodies against albumin and haemoglobin adducts were performed. Antibodies of the immunoglobulin (Ig) isotypes A, G, and M against acetaldehyde-adducts are formed in patients with prolonged heavy alcohol consumption. IgA titres in ALD patients are significantly higher than those found in patients with non-alcoholic liver disease, non-drinking controls, or heavy drinkers with no signs of liver disease. Anti-adduct IgG titres, in turn, are increased both in ALD and in heavy drinkers with no signs of liver disease as compared with non-alcoholic liver disease patients or non-drinking controls. It appears that anti-adduct IgA, IgG and IgM titres in ALD patients correlate with the severity of the liver disease. Although this association is a limitation for the usefulness of these antibodies as markers of alcohol abuse, it may serve as a basis for the differential diagnosis of alcohol-induced liver disease.

In order to further elucidate the interaction between CD23 and β2 integrins (CD11b/CD18) the following objectives were established: Expression and purification of CD11b I-domain as a GST-fusion protein using Escherichia coli; Cloning, synthesis and expression of CD18 I-Like domain.CD11b I-domain has previously been expressed as a GST-fusion protein (Daniels, 2010) and consequently led to comparable expression of CD18 I-like domain as a GST-fusion protein; Preparation of two site-directed mutants of CD18 I-Like domain in order to study the function of the serine residue involved in the S116P mutation. The serine was mutated to proline, as in LAD patients, as well as alanine, a non-polar alternative, in order to contrast and compare binding characteristics.  Expression, refolding and purification of sCD23, and a double mutatedsCD23 (RKΔAA) from E. coli; This was performed according to the method described by Daniels et al. (2005); Investigation of the CD23-CD11b I-like domain interaction through surface plasmon resonance spectroscopy.