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Background/Purpose: ADHD is frequently associated with functional deficits across social, familial, and academic contexts (Pelham, 2005). Children with ADHD and internalizing symptoms typically experience higher levels of functional impairment compared to their counterparts with exclusively ADHD (Bishop et al., 2019). Positive parenting practices and child strengths have been found to play a protective role for children with ADHD (Healy et al., 2011; McCrimmon et al., 2018). This study aims to investigate the influence of internalizing symptoms on functional impairment for children with ADHD. Child strengths and parenting strengths have been examined to identify moderating effects of the aforementioned constructs. Methods: Data were collected in a community mental health centre using the Child and Adolescent Needs and Strengths questionnaire (CANS) and the Child Strengths and Difficulties questionnaire (SDQ). Participants included 209 children and their caregivers seeking mental health services between the ages of 5 and 11 with an ADHD diagnosis. To examine the moderating effects of parenting and child strengths, ordinary least squares regression models were tested using the PROCESS macro for SPSS (v3.5; Hayes, 2018). Results: Results suggest that levels of internalizing symptoms influence functional impairment in children with ADHD. Child strengths moderate the relationship between internalizing symptoms and functional impairment when internalizing symptoms are medium to high. Conclusion: Findings from this study demonstrate that facilitating child strengths can help moderate functional impairment for children who experience ADHD and internalizing symptoms.

Using data from the Health, Aging, and Body Composition study, we examined whether low 25-hydroxyvitamin D (25[OH]D) concentrations were associated with prevalent or incident cognitive impairment. Serum 25(OH)D concentrations were measured in 2,786 older adults and categorized as <20 ng/mL, 20 to <30 ng/mL, or ≥30 ng/mL. Cognitive impairment was defined as a score >1.5 standard deviations below race and education specific means on either digit symbol substitution test or modified mini-mental state test. Logistic regression determined the odds of cognitive impairment at baseline and year 5 by 25(OH)D category. 25(OH)D concentrations were <30 ng/mL in 57.3% of whites and 84.6% of blacks. After excluding participants with baseline cognitive impairment (n = 340), 13% of whites and 13% of blacks developed cognitive impairment by year 5. In whites, 25(OH)D concentrations <30 ng/mL were not associated with prevalent or incident cognitive impairment. Black participants with 25(OH)D concentrations <20 ng/mL had a higher odds of prevalent, but not incident cognitive impairment (OR (95% CI): 2.05 (1.08–3.91), p = 0.03) compared to participants with 25(OH)D concentrations ≥30 ng/mL. Low 25(OH)D concentrations were associated with twofold higher odds of prevalent cognitive impairment in blacks.

Introduction: Hearing impairment occurs when a child has hearing loss greater than 30dB in their better hearing ear and an adult cannot detect sound lower than 40dB in the better hearing ear. It is a common sensory disorder that affecting approximately 360 million worldwide, with an incidence of 6 in 1000 live births in developing countries such as those in Sub-Saharan Africa. 50 % of hearing impairment, in developed countries, is due to genetic factors, with 70% of genetic hearing impairment being classified as non-syndromic hearing impairment, which occurs when the hearing impairment presents with no other clinical manifestations. Hearing impairment is associated with over 150 genes, of which two connexin genes, GJB2 and GJB6, are the most prevalent genes associated with hearing impairment in European, Asian and North American of European ancestries populations. These genes have however been shown to be insignificant causes of Hearing Impairment in African populations. Aim: The aim of this study is to determine the rates for putative pathogenic variants in 172 hearing impairment associated genes, among Cameroonian patients affected by hearing impairment, and non-hearing-impaired controls. Methods: Patients and controls Patients were recruited from various schools of the Deaf and Ear, Nose and Throat (ENT) clinics in Cameroon. The patients were examined by qualified medical geneticists and ophthalmologist and detailed family history and medical history was obtained from the patients and their parents. 19 patients, who were negative for GJB2 and GJB6 mutations and presented with putative non-syndromic hearing impairment, were selected from a cohort of 582 patients for the present study. The control population consisted of 130 ethnically matched groups without any personal or familial history of hearing impairment. The controls were recruited from Yaoundé Central Hospital and Laquintinie Hospital in Cameroon. Whole exome sequencing DNA was extracted from whole blood using the salting out procedure and the Puregene Blood kit®. The DNA was subjected to spectrometry and gel electrophoresis to determine the quantity and quality of the DNA samples. The samples were then subjected to whole exome sequencing on the Illumina platform using the Nextera Rapid Capture Exome Kit at an average read depth of 30X, whereby only 18 patients were successfully sequenced. The exomes were then subjected to FastQC and SolexaQC++ for quality control measures and aligned to the hg19 reference genome using GATK and VariantMetaCaller. Bioinformatics analysis Variant annotation was performed using Annovar and the annotated variants were filtered based in rarity and pathogenicity. Tests for genetic differentiation and principle component analysis was performed on the combined patient exomes and combine control exomes. The first principle component analysis included data from African populations from the 1000 Genomes Phase 3 as well as six control samples from the Democratic Republic of Congo; and the second principle component analysis analysed on the Cameroonian patients and control population. Population structure analysis was followed by protein-protein interaction analysis using custom python and R script and pathway enrichment analysis using Enrichr combined with a second custom R script. The proportion of derived and ancestral alleles was computed by downloading the SNP ancestral alleles from Ensembl and verifying the presence of the SNPs in dbSNP database. The combined patient and control exomes were annotated using the VCFtools “fillOaa” script. The ancestral alleles were computed by dividing the number of times the alternative allele matched the ancestral allele with the number of copies of all the alternative alleles across all samples at the particular position. The ancestral alleles were categorised into six bins, based on their minor allele frequency, in the patient and control populations and this was used to contrast their proportions of derived and ancestral alleles. Furthermore, the proportion of ancestral and derived alleles in hearing impairment associated genes was computed at SNP based level for the Cameroonian population and contrasted with population from the Democratic Republic of Congo. Variants validation by Sanger sequencing Primers were designed to amplify the fragment surrounding the purported SNPs in MYO15A, MYO3A, and COL9A3 as well as for the fragments surrounding the population specific SNPs in VTN, RPL3L and DHRS4L2. Polymerase chain reaction was performed for the MYO15A, and MYO3A fragments. This was followed by purification of the PCR products and direct cycle Sanger sequencing of the PCR products. The sequencing products were then purified through ethanol precipitation and the fragments were suspended in HiDi Formamide and run on the capillary electrophoresis. The variants in MYO3A, MYO15A and COL9A3 were viewed in Integrated Genomics Viewer using the Bam files as well. Results Putative deleterious variants Single nucleotide polymorphism (SNPs) in MYO3A, MYO15A and COL9A3, were filtered out as putative causative mutations for three, four and two patients respectively. Direct Sanger Sequencing and viewing the patients BAM files did not confirm the presence of any of these putative pathogenic in the patients. Variations in USH2A, HSD17B4 and MYO1A were also filtered out but these variants were not considered disease causing, after a careful genotype to phenotypes correlations. Population genetics variants differentiations At a population level, specific variations were identified in FOXD4L2, DHRS2L6, RPL3L and VTN. Significant genetic differentiation was shown to exist between the control population and the patients’ population with regard to specific variants in VTN and RPL3L; furthermore, it was shown that these variants in VTN and RPL3L interact with other hearing impairment associated proteins with evidences that that VTN is hub protein for a hearing impairment associated pathway along with nine other genes. Conversely, this was not the case for variants described in FOXD4L2 and DHRS2L6. In known hearing Impairment genes, the proportion of ancestral alleles was lowest for the patients’ population for variations with minor allele frequencies between 0.0 and 0.1. The proportion of derived and ancestral alleles was also shown to differ between the Cameroonian and the population from the Democratic Republic of Congo, indication possible regional differences in aetiology of Hearing impairment amongst multiple African populations. Discussion Low putative pathogenic variants in known hearing impairment genes among Africans The low pick up rate for putative pathogenic variants in our patients follows a similar trend observed in the African American populations, with hearing impairment, as well as data from targeted exome sequencing from South African and Nigerian populations. This result is also in agreeance with other studies that interrogated hearing impairment in African populations utilising other means besides next generation sequencing. This result also highlights the importance of validating any results obtained from next generation sequencing through traditional approaches such as Sanger Sequencing or viewing the BAM files on IGV, specifically in African population, poorly represented in Exome databases. Bioinformatics Analysis Exhibited some Specific Variants among Cameroonian Protein-protein interactions and enrichment analysis indicated that VTN and RPL3L, and their interacting proteins, are significantly associated with osteoclast differentiation, which is associated with hearing impairment in osteogenesis imperfecta. VTN was further shown as a hub protein of a protein subnetwork, along ATPB2. The presence of a second protein acting as a hub protein may account for why aberrations in VTN have not been associated with a disease; whereby ATPB2 may ameliorate the pathogenic phenotype that ought to be observed in the presence of null mutations in VTN. Evolutionary adaptation of human hearing Data indicates the patient population carried a higher proportion of derived alleles in known hearing impairment genes, at low minor allele frequencies; possibly indicating, the interactive modifiers capacities of multiple hearing impairment genes, or alternatively, the polygenetic nature of hearing impairment in some patients. The proportion of ancestral and derived alleles was contrasted in the Cameroonian and the population from the Democratic Republic of Congo and it indicated that the variations that may result in hearing impairment in the one population may not be the same variations that result in hearing impairment in the other population Due to this, it is necessary to determine the causative variants resulting in disease in each of these populations independently. Conclusion and perspectives The results support a low pick up rate of putative variants in 172 known genes in groups of Cameroonian patients with HI, underscoring the current Targeted panel sequencing for HI may not be relevant for some African populations. The result also support the need of confirmation of variants found in WES, as well careful genotype to phenotypes correlations, particularly among African, whose sequences exome is relatively low in Exomes databases, and as a result could lead to more false positive results. Population genetic analysis has provided novel insight in the genetic architecture of HI among this group of Africans; particularly, the differential frequencies of ancestral alleles vs derived alleles in HI genes among patients vs controls underline the possibility of multigenic influence on the phenotype of Hearing Impairment that have not been well investigated, and may also signal evolutionary enrichment of some variants of HI genes in the populations as the result of natural selections, that deserve further investigation. The result supports the need of intensive familial studies in multiple African populations in order to unravel the novel genes and those variants that are relevant in clinical practice for people of African ancestry.

In South Africa, approximately 600 000 individuals are visually impaired. Approximately onethird of genetic disorders and syndromes involves the eye, including conditions such as congenital cataracts, glaucoma, albinism, and retinal degenerative disorders. The transition into adulthood of visually disabled individuals is a crucial time, as it lays the foundation for their future. The aim of this research was to explore the level of understanding, perceptions and lived experiences of young adults aged eighteen to twenty-three who are visually impaired due to a genetic cause. A qualitative design, utilizing a phenomenological approach was used for this study. Fifteen participants were recruited through Athlone School for the Blind, the League of Friends of the Blind and Retina SA. In-depth interviews were conducted and data obtained was analysed using thematic analysis. Five themes were identified through this approach indicating the implications of having a genetic visual condition as perceived and experienced by these young adults. Most of the young adults experienced difficulty in understanding their condition and the genetic bases thereof and they had a strong desire to obtain clarity and knowledge via genetic counselling. The community was thought not to understand their situation either. They were unsure of the inheritance risks to future offspring and some indicated that they felt that this was a gamble they were unwilling to take, whilst others would have children. In some instances, their own visual impairment might create obstacles to raising children. Social interactions were greatly impacted and they felt isolated and tried to avoid unpleasant treatment, stigmatization and pity from the community. Intimate relationships were also noted as a challenge. Mobility is a major obstacle due to the incapacity to drive, as well as the lack of disability user-friendly public transportation and a daunting environment. They want to and feel that they can be independent and achieve the same things as sighted individuals, but society and life circumstances often create barriers to this. This research could assist in providing information to create more efficient, patient-centred genetic services and might be informative to various organizations about targeted support to provide these individuals and methods to assist their transition to adulthood.

Introduction: Balance impairment is a commonly reported symptom of concussion. Very little research has been undertaken regarding the complexities of balance impairment after concussive injury in the pediatric population. Purpose: The objectives of this study were: 1) to identify the factors that are associated with balance impairments in children with concussion; 2) to review and evaluate four commonly used balance measures; and 3) to provide recommendations for the best methods of clinical evaluation of balance in children and youth after concussive injury. Methods: A cross-sectional balance evaluation was completed on 104 children, ages 5-18 who had a confirmed diagnosis of concussion. Four balance measures were examined to determine which was the most appropriate for evaluating balance post-concussion. Results: 37% of children were found to have balance impairment according to the BOT-2. The full logistic regression model was found to be not statistically significant however, mechanism of injury with an odds ratio of 2.1 and 95% CI [0.810, 5.027] indicates that children with sport-related injuries are twice as likely to have balance impairment than those with a non-sports-related injury. Chi-Square analyses showed a statistically significant association for mechanism of injury (χ2=11.05, p = 0.03) and age (χ2 = 0.04, p =-0.02) for children who presented with balance impairments and those who did not. Children with balance impairment may present with different symptom profiles than children without. Conclusion: Using a single method of assessment may not provide an accurate representation of balance impairment in children and youth after concussion. Based on the comparison of measurement properties and application of each measure to 104 children with concussion, the BOT-2 and the CB&M when used together provide the most comprehensive assessment of balance and postural instability in children with concussive injury. Age, mechanism of injury and site of impact may be leading factors in the development of balance impairment. / Thesis / Master of Science Rehabilitation Science (MSc)

<p><em><strong>Bakgrund:</strong></em> Efter införandet av IFRS har det förekommit en del förändringar gentemot tidigare redovisnings regler i Sverige. Från och med 1 januari år 2005 skall alla börsbolag gå över till IFRS 3 <em>Business Combinations</em>. Detta innebär att goodwill inte får skrivas av så som det gjorts tidigare. Nu mer måste det årligen ske ett Impairment test av goodwill för att undersöka om det föreligger ett nedskrivningsbehov.Diskonteringsräntan används vid impairment test av goodwill och är en viktig faktor vid nuvärdesberäkningar av kassaflöden.</p><p><strong><em>Problem:</em></strong> Noterade börsbolag utför ett <em>impairment test</em> av goodwill, vilket görs för att undersöka om nedskrivning av goodwill är aktuellt eller inte. Diskonteringsräntan är en viktig faktor vid Impairment test. Trots detta test utför inte de flesta företag nedskrivningar.</p><p>Varför gör inte företag några nedskrivningar kan det bero på att en praxis utvecklats?</p><p>Hur bestämmer företag diskonteringsräntan och varför, vilka är dess komponenter? </p><p><strong><em>Syfte:</em></strong> Med denna undersökning är att beskriva systematiskt hur företag tar fram sina valda diskonteringsräntor för omprövning av goodwill, kan detta bero på en praxisutveckling eller av andra omständigheter. Vi vill även undersöka vilka metoder företag använder sig för att ta fram dess diskonteringsränta samt dess komponenter.</p><p><strong><em>Metod:</em></strong> Undersökningen i denna uppsats har främst utgått från en kvantitativ enkätundersökning, även vetenskapliga tidskrifter samt skriftliga källor har använts.Totalt kontaktades 108 stycken noterade företag på Stockholms börs.</p><p><strong><em>Slutsatser:</em></strong> Det har visat sig att företag använder sig av förväntade kassaflöden vid framtagning av dess diskonteringsränta och WACC modellen. Impairment test har visat sig vara av betydelse då det ger en mer rättvisande bild och överblick på goodwill.</p> / <p><strong><em>Background</em>:</strong> Following the introduction of IFRS, has given rise to some changes towards the previous accounting rules in Sweden. As of January 1 year 2005, all listed companies move to IFRS 3 Business Combinations. This means that goodwill is not to be amortized as done previously. Now annually there has to be an Impairment test of goodwill in order to investigate whether there is need for impairment or not. The discount rate is used for impairment testing of goodwill and is an important factor in calculating the discounted value of cash flows.</p><p><em><strong>Problem</strong></em><em>:</em> Public listed company doing an impairment test of goodwill is done to examine whether the impairment of goodwill is relevant or not. The discount is an important factor in Impairment test. Although it's showed that most companies do not need to write down goodwill. Why do not some companies write-down goodwill, is it maybe that a practice developed? How do companies discount and why? What are its components?</p><p><em><strong>Purpose</strong></em>: This study is to describe systematically how firms develop their selected discount rates for review of goodwill, is it maybe due to a practice development or other circumstances. We also want to explore the methods companies use to develop its discount rate and its components.</p><p><em><strong>Method</strong></em>: The study in this thesis has mainly been based on a quantitative survey, including scientific journals and written sources. 108 companies listed on the Stockholm stock exchange were contacted.</p><p><em><strong>Conclusions</strong></em>: It has been shown that companies use the expected cash flows at the development of its discount rate and WACC model. Impairment tests have also proved to be of importance since it gives a more accurate picture and overview of goodwill.</p>