Please read the abstract in the section 00front of this document / Thesis (PhD (Agronomy))--University of Pretoria, 2007. / Plant Production and Soil Science / unrestricted

With the high rate of human population growth in Africa, it is imperative that food production be increased through improving yields and bringing more land under cultivation. Nitrogen is a key element required for plant growth and with the low input of fertilizers into African farming-systems, it is necessary to harness biological nitrogen fixation (BNF) to its fullest extent. To maximize nitrogen fixation in crop and pasture legumes under various conditions and cropping systems requires intensified research. In this study the diversity of root nodule bacteria associated with Lotononis species in South Africa was investigated. To our knowledge, no past attempts have been made to investigate the diversity of rootnodule-bacteria associated with the genus Lotonollis. Eighty nodulated plants representing thirty-two Lotononis spp. were collected from all the main geographical and climatological regions in South Africa. Isolates obtained from rootnodules were purified and characterized with sodium dodecyl-sulphate gel polyacrylamide gel electrophoresis (SDS-PAGE), supplemented at the genomic level with 16S rDNA sequence data of selected strains. Initial screening (SDS-PAGE) showed that most isolates obtained from the same plant species, grouped into various clusters within the dendrogram. A few of the isolates from similar host plants seemed to cluster with high similarity. It is thus reasonable to conclude that host specificity in the symbiotic interaction is less applicable for most species of Lotononis. When considering the geographical origins of the isolates, their diverse nature was clearly illustrated. It was showed that isolates from similar geographical regions were evenly distributed throughout the dendrogram. Some of the isolates obtained from arid environments formed closely related electrophoretic groups. It was subsequently shown that root-nodule bacteria associated with Lotononis species are not restricted to a particular rhizobial genus, but that heterogeneity is evident. Some of the isolates were also related to genera outside the Rhizobiaceae, namely Methylobacterium and Burkholderia. / Dissertation (MSc (Microbiology))--University of Pretoria, 2006. / Microbiology and Plant Pathology / unrestricted

The challenge of food security requires that agricultural production is no longer based on a narrow genetic material present in conventional crops. Whereas conventional crops have been genetically improved to suit management practices of the modern farmer, the future farmer requires that there be access to a wide variety of genetic material for economic exploitation and to respond to the challenges of climate change in a sustainable fashion. This study was designed to learn about production of wild water melon [Citrullus lanatus (Thunb.) Matsumura and Nakai] from seed germination, seedling establishment and field crop production. The specific objectives of the study were (a) to determine the effect of water stress on three landraces of watermelon differing in seed colour and provenance during seedling establishment, (b) to determine the effect of planting date on crop growth and yield under field conditions, and (c) to relate proline accumulation to water stress in wild watermelon. Three seedlots, ‘B’, ‘DB’ and ‘VDB” were derived from seeds collected from subsistence farming communities of the Eastern Cape, and KwaZulu-Natal. Following one season of seed production in Pietermaritzburg, KwaZulu-Natal, seeds were tested for germination capacity, before seedlot response to water stress was determined in three substrates made of pine bark, a 1:1 mixture of fine sand and pine bark and fine sand only. The substrates were kept at 75% FC, 50% F.C and 25% F.C., to create varying levels of water regimes during 12 weeks of seedling growth in a glasshouse (16/21oC (day/night) and 60% RH). Leaf proline content was determined at seedling harvest. Crop production under field conditions occurred at one site with three planting dates late September 2008, November 2008 and January 2009, respectively. There were significant differences among seedlots with respect to seed quality and seedling yield, which consistently showed that B > VDB > DB. The differences in seedlots continued in the same order even in response to field conditions. Wild watermelon was responsive to water stress during seedling growth, but high water regimes compromised water use efficiency. Proline accumulation correlated with water stress. The best plant growth and yield under field conditions was obtained when planting occurred in September, followed by November and January plantings, respectively. Early planting was also associated with high crop growth rate and larger fruit size. It is concluded that despite being a desert crop, wild watermelon responds to water deficits during seedling growth. Results of field studies cannot be conclusively used to determine crop response to water stress, although they gave a good indication of crop response to different conditions of rainfall and temperature at the study site from September to March. / Thesis (M.Sc.Agric.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

MA (African Studies) / Centre for African Studies / This study explored indigenous medicinal knowledge of Phonda in the Vhembe District. It has been noticed that phonda is becoming a scarce crop. The objectives entail seeking to solicit information on the uses of phonda, to understand the attitudes of local households towards phonda and to establish what can be done to restore the cultivation of phonda. The study used qualitative method involving unstructured interviews to collect and thematically analyse data. Purposive sampling and snowballing methods were used to make up the study sample. From literature, it is known that traditional health practitioners used phonda for treatment of certain diseases and illnesses. Phonda is no longer common, but the study sought to encourage the cultivation of phonda. The study employed an Afrocentric paradigm of social change as the conceptual framework. The study found that many households no longer plough phonda as they used to in the past. The study recommends that people should be educated on the importance of phonda to encourage them to plough it. Additionally, the researcher recommends further studies into different purposes of phonda and areas to boost its farming.