Até pouco tempo acreditava-se que a maioria das moléculas de RNA estava relacionada à tradução de proteínas. Porém, descobriu-se que outros tipos de moléculas de RNA que não são traduzidas estão presentes em muitos organismos diferentes e afetam uma variedade de processos moleculares, são os chamados RNAs não-codificantes (ncRNAs). Apesar de sua importância funcional, os métodos biológicos e computacionais para a detecção e caracterização de RNAs não-codificantes ainda são imprecisos e incompletos. A identificação de novas espécies de ncRNAs é difícil através de procedimentos experimentais e as técnicas computacionais existentes são lentas. O objetivo deste trabalho foi obter uma ferramenta mais eficiente para a comparação de uma seqüência de RNA não-codificante contra um banco de seqüências. Para isso foi proposto e implementado um modelo para identificação computacional de ncRNAs com apoio dos pacote Viena e Infernal e foram realizados experimentos para avaliá-lo / Until recently it was generally accepted that most RNA molecules were involved in the translation process. However, it was discovered that many types of untranslated RNA molecules are present in many different organisms and they are related to a wide variety of molecular processes. These molecules are called non-coding RNAs (ncRNAs). Despite their functional importance, the biological and computational methods to detect and identify non-coding RNAs are still imprecise and incomplete. The discovery of new ncRNAs species is difficult through experimental procedures and the existing computational techniques are slow. This project aimed at obtaining a more efficient tool that compares a non-coding RNA sequence against a sequence database. In order to achieve this, a computational model for ncRNAs identification using the Vienna and Infernal packages has been proposed and implemented. Experiments were conduced to evaluate the model

Até pouco tempo acreditava-se que a maioria das moléculas de RNA estava relacionada à tradução de proteínas. Porém, descobriu-se que outros tipos de moléculas de RNA que não são traduzidas estão presentes em muitos organismos diferentes e afetam uma variedade de processos moleculares, são os chamados RNAs não-codificantes (ncRNAs). Apesar de sua importância funcional, os métodos biológicos e computacionais para a detecção e caracterização de RNAs não-codificantes ainda são imprecisos e incompletos. A identificação de novas espécies de ncRNAs é difícil através de procedimentos experimentais e as técnicas computacionais existentes são lentas. O objetivo deste trabalho foi obter uma ferramenta mais eficiente para a comparação de uma seqüência de RNA não-codificante contra um banco de seqüências. Para isso foi proposto e implementado um modelo para identificação computacional de ncRNAs com apoio dos pacote Viena e Infernal e foram realizados experimentos para avaliá-lo / Until recently it was generally accepted that most RNA molecules were involved in the translation process. However, it was discovered that many types of untranslated RNA molecules are present in many different organisms and they are related to a wide variety of molecular processes. These molecules are called non-coding RNAs (ncRNAs). Despite their functional importance, the biological and computational methods to detect and identify non-coding RNAs are still imprecise and incomplete. The discovery of new ncRNAs species is difficult through experimental procedures and the existing computational techniques are slow. This project aimed at obtaining a more efficient tool that compares a non-coding RNA sequence against a sequence database. In order to achieve this, a computational model for ncRNAs identification using the Vienna and Infernal packages has been proposed and implemented. Experiments were conduced to evaluate the model

Depuis son écriture en 1480, le Baratre infernal de Regnaud le Queux reste, à ce jour, une œuvre méconnue, dont l'aspect littéraire est principalement étudié. Cet imposant volume rassemble les informations relatives à l'enfer de l'Antiquité au Moyen Age, créant une véritable encyclopédie de l'enfer où se mêlent prose et vers, latin et moyen français, antique et médiéval. Nous proposons une recherche multidisciplinaire concentrée sur un extrait défini, à savoir les folios 127 à 143, dans lesquels le poète discute de la structure de l'enfer et fait une description du diable et des démons. Nous avons effectué une analyse codicologique et paléographique du manuscrit Paris, BnF, fr. 450, en plus d'une analyse littéraire en deux temps consacrée aux folios 127 à 143 : nous nous intéressons dans un premier temps à la structure du passage à l'étude et la stylistique de l'auteur ; nous examinons dans un second temps les auctoritates déployées par Regnaud et leur usage au sein de son discours. L'objectif, après avoir réalisé une édition des folios à l'étude, est d'approfondir la connaissance acquise sur ce manuscrit et d'exposer l'harmonie délicate entre la séparation et le mariage d'éléments opposés qu'instille l'auteur. Le fruit de notre recherche est donc une édition partielle du manuscrit, ainsi qu'une analyse détaillée du prosimètre et des sources employées par Regnaud pour soutenir son discours. Le choix de restreindre l'étude à un extrait qui n'a pas été étudié jusqu'à aujourd'hui permet un nouvel angle précis d'interprétation qui contribue à la compréhension globale de l'œuvre. On comprend soit dit au passage que les opportunités de recherches relatives à cette œuvre médiévale ne manquent pas. / Since it has been written in 1480, Regnaud le Queux's Baratre infernal remains, to this day, a relatively unknown work. The studies are mainly on its literary aspect. This imposing volume gathers information about hell from Antiquity to the Middle Ages, creating an authentic encyclopedia on hell, in which prose and poetry, Latin and French, the antique and the medieval coexist. We suggest a multidisciplinary research dedicated to a studied excerpt of the manuscript Paris, BnF, fr. 450 : the folios 127 to 143, in which the poet elaborates on hell's structure and describes the devil and demons. We use three different approaches: codicology and paleography help us understand the manuscript's context of production and its purpose. We also do a literary analysis around the folios 127 to 143 in two steps: firstly, we observe the poetic structure and the author's style ; secondly, we examine the auctoritates used by Regnaud, and their application in his discourse. The objective, after rendering an edition of the studied passage, is to gain knowledge about this manuscript. Furthermore, we expose the intricate harmony between the union and separation of disparate elements infused by the author : prose cohabits with poetry, the Ancients coexist with the medieval authors, just as Latin and French. Thus, this study's results is a partial edition of the manuscript, and a detailed analysis of the prosimetra and the sources deployed by the poet. Focusing on a delimited and until now unstudied passage gives place to a new and precise interpretation, which contributes to the global comprehension of the volume. Ultimately, so much more can still be done regarding this medieval opus.

Depuis son écriture en 1480, le Baratre infernal de Regnaud le Queux reste, à ce jour, une œuvre méconnue, dont l'aspect littéraire est principalement étudié. Cet imposant volume rassemble les informations relatives à l'enfer de l'Antiquité au Moyen Age, créant une véritable encyclopédie de l'enfer où se mêlent prose et vers, latin et moyen français, antique et médiéval. Nous proposons une recherche multidisciplinaire concentrée sur un extrait défini, à savoir les folios 127 à 143, dans lesquels le poète discute de la structure de l'enfer et fait une description du diable et des démons. Nous avons effectué une analyse codicologique et paléographique du manuscrit Paris, BnF, fr. 450, en plus d'une analyse littéraire en deux temps consacrée aux folios 127 à 143 : nous nous intéressons dans un premier temps à la structure du passage à l'étude et la stylistique de l'auteur ; nous examinons dans un second temps les auctoritates déployées par Regnaud et leur usage au sein de son discours. L'objectif, après avoir réalisé une édition des folios à l'étude, est d'approfondir la connaissance acquise sur ce manuscrit et d'exposer l'harmonie délicate entre la séparation et le mariage d'éléments opposés qu'instille l'auteur. Le fruit de notre recherche est donc une édition partielle du manuscrit, ainsi qu'une analyse détaillée du prosimètre et des sources employées par Regnaud pour soutenir son discours. Le choix de restreindre l'étude à un extrait qui n'a pas été étudié jusqu'à aujourd'hui permet un nouvel angle précis d'interprétation qui contribue à la compréhension globale de l'œuvre. On comprend soit dit au passage que les opportunités de recherches relatives à cette œuvre médiévale ne manquent pas. / Since it has been written in 1480, Regnaud le Queux's Baratre infernal remains, to this day, a relatively unknown work. The studies are mainly on its literary aspect. This imposing volume gathers information about hell from Antiquity to the Middle Ages, creating an authentic encyclopedia on hell, in which prose and poetry, Latin and French, the antique and the medieval coexist. We suggest a multidisciplinary research dedicated to a studied excerpt of the manuscript Paris, BnF, fr. 450 : the folios 127 to 143, in which the poet elaborates on hell's structure and describes the devil and demons. We use three different approaches: codicology and paleography help us understand the manuscript's context of production and its purpose. We also do a literary analysis around the folios 127 to 143 in two steps: firstly, we observe the poetic structure and the author's style ; secondly, we examine the auctoritates used by Regnaud, and their application in his discourse. The objective, after rendering an edition of the studied passage, is to gain knowledge about this manuscript. Furthermore, we expose the intricate harmony between the union and separation of disparate elements infused by the author : prose cohabits with poetry, the Ancients coexist with the medieval authors, just as Latin and French. Thus, this study's results is a partial edition of the manuscript, and a detailed analysis of the prosimetra and the sources deployed by the poet. Focusing on a delimited and until now unstudied passage gives place to a new and precise interpretation, which contributes to the global comprehension of the volume. Ultimately, so much more can still be done regarding this medieval opus.