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Thèse de doctorat--Sciences naturelles--Paris--Faculté des sciences, 1872. N°: 340.

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Barley row-type describes the number of grains present at a node on the barley spike. Two forms exist amongst cultivated barley: two-rowed with only the central of three spikelets fertile producing a single grain at a node and six-rowed with all three spikelets fertile, producing three grain at a node. Twelve regions of the barley genome have been associated with the row-type character with specific genes identified at three loci, <i>VRS1</i>, <i>VRS4</i> and <i>INT-C</i>. Advancements in the understanding of the genetic control underpinning barley row-type enables the identification of potential mechanisms for improving yield and yield architecture within the cereals.  This study used genetic linkage mapping in segregating F2 populations to refine the genetic location of the row-type locus, <i>VRS3</i>, to 16 candidate genes on barley chromosome 1H. Sequencing candidate loci in 32 <i>vrs3</i> induced mutant alleles identified <i>VRS3</i> to be a highly conserved JmjC histone demethylase, with two natural alleles within European cultivated barley. <i>VRS3</i> was further characterised as a potential means of improving grain uniformity within cultivated six-rowed barley, through phenotypic assessment of grain size in varying allele combinations of <i>VRS3</i>, <i>VRS1</i> and <i>INT-C</i>. The addition of six-rowed alleles at these loci was found to improve balance between central and lateral grain parameters, resulting in a more uniform grain sample. Analysis of gene expression found <i>Vrs3</i> to be constitutively expressed across a diverse panel of barley tissues. Moreover, detailed study within the developing inflorescence suggests a role for <i>Vrs3</i> in the regulation of the row-type genes <i>VRS1</i> and <i>INT-C. </i>

Heterosis, the phenotypic superiority of first generation progeny over that of its inbred parents, has been extensively investigated. However, differences in the phenology and dynamic growth patterns between parents and offspring provide challenges in understanding causal factors behind superior trait values. For maize, manipulation of heterosis to increase grain yield has been of primary importance, and the number of spikelets that develop on the female inflorescence is the primary determinant of grain yield. The initial experiment examined heterosis in genetic backgrounds that led to minimal differences in phenology and plant architecture. Growth curves were used to characterize the dynamic expression of heterosis between the hybrid and the inbred parents for a series of vegetative and reproductive traits across stages of development. The second experiment was conducted to determine the effects that stress due to planting density might have on the number of properly developed spikelets, as the first experiment, along with results from the literature, provided evidence to suggest that a proportion of the later forming spikelets found on the distal portion of the female inflorescence were not capable of producing kernels. Results from the initial experiment suggested that expression of heterosis for individual characteristics – such as fresh weight, whose percent mid-parent heterosis was 82% at V4 and declined steadily to 17% at V11 – begins at a high level and decreased during development. On a whole plant level – as determined by a combined analysis of the values and growth rates of the individual characteristics – heterosis increases throughout development until it reaches a steady-state level. Results from the second experiment indicated that increasing plant density did not affect the total number of spikelets per ear but decreased the number of kernels per ear, and it was found that the number of properly developed spikelets per ear was equal to the number of kernels per ear in eight of the nine genotypes tested. Optimal growth stages for more in-depth investigation of transcriptomic changes that may identify causal genetic factors of heterosis for yield were not found, and stress increases the proportion of improperly developed spikelets causing a loss in kernel number. / Natural Sciences and Engineering Research Council of Canada, Ontario Ministry of Agriculture, Food and Rural Affairs, Canadian Foundation for Innovation, and Ontario Innovative Trust

[ES] Las leguminosas son un grupo de plantas consideradas de gran importancia por su valor nutricional para la alimentación humana y ganadera. Además, las familias de leguminosas se caracterizan por rasgos distintivos de desarrollo como su inflorescencia compuesta y su compleja ontogenia floral. Para comprender mejor estas características distintivas, es importante estudiar los genes reguladores clave involucrados en el desarrollo de la inflorescencia y la flor. El gen SUPERMAN (SUP) es un factor transcripcional de dedos de zinc (Cys2-Hys2) considerado como un represor activo que controla el número de estambres y carpelos en A. thaliana. Además, SUP está involucrado en la terminación del meristemo floral y el desarrollo de los tejidos derivados del carpelo. El objetivo principal de este trabajo fue la caracterización funcional del ortólogo de SUP en la leguminosa modelo Medicago truncatula (MtSUP). Logramos este objetivo en base a un enfoque de genética reversa, análisis de expresión génica y ensayos de complementación y sobreexpresión. Nuestros resultados muestran que MtSUP es el gen ortólogo de SUP en M. truncatula. MtSUP comparte algunos de los roles ya descritos para SUP con algunas variaciones. Curiosamente, MtSUP controla la determinación del meristemo inflorescente secundario (I2) y de los primordios comunes (CP) a pétalos y estambres. Por tanto, MtSUP controla el número de flores y de pétalos-estambres que producen el meristemo I2 y los primordios comunes, respectivamente. MtSUP muestra funciones novedosas para un gen de tipo SUP, desempeñando papeles clave en los meristemos que confieren complejidad de desarrollo a esta familia de angiospermas. Este trabajo permitió identificar a MtSUP, un gen clave que forma parte de la red reguladora genética que subyace al desarrollo de la inflorescencia compuesta y de las flores en la leguminosa modelo M. truncatula. / [CA] Les lleguminoses són un gran grup de plantes considerades de gran importància pel seu valor nutricional per a l'alimentació humana i ramadera. A més, les famílies de lleguminoses es caracteritzen per trets distintius de desenrotllament com la seua inflorescència composta i la seua complexa ontogènia floral. Per a comprendre millor estes característiques distintives, és important estudiar els gens reguladors clau involucrats en la inflorescència i el desenrotllament floral. El gen SUPERMAN (SUP) és un factor transcripcional de dits de zinc (Cys2-Hys2) considerat com un repressor actiu que controla el nombre d'estams i carpels en A. thaliana. A més, SUP està involucrat en la terminació del meristemo floral i el desenrotllament dels teixits derivats del carpel. "L'objectiu principal d'este treball va ser la caracterització funcional de l'ortòleg de SUP en la lleguminosa model Medicago truncatula (MtSUP) . Aconseguim l'objectiu amb base en un enfocament genètic invers, anàlisi d'expressió gènica i assajos de complementació i sobreexpressió. Els nostres resultats mostren que MtSUP és el gen ortòleg de SUP en M. truncatula. MtSUP compartix alguns dels rols ja descrits per a SUP amb variacions. Curiosament, MtSUP està involucrat en la determinació del meristemo de la inflorescència secundària (I2) i els primordios comuns (CP). Per tant, MtSUP controla el nombre de flors i pètals-estams que produïxen el meristemo I2 i els primordios comuns, respectivament. MtSUP mostra funcions noves per a un gen tipus SUP, exercint papers clau en els meristemos que conferixen complexitat de desenrotllament a esta família d'angiospermes. "Este treball va permetre identificar a MtSUP, un gen clau que forma part de la xarxa reguladora genètica darrere de la inflorescència composta i el desenrotllament de flors en la lleguminosa model M. truncatula. / [EN] Legumes are a large group of plants considered of great importance for their nutritional value in human and livestock nutrition. Besides, legume families are characterized by distinctive developmental traits as their compound inflorescence and complex floral ontogeny. For a better understanding of these distinctive features is important to study key regulatory genes involved in the inflorescence and floral development. The SUPERMAN (SUP) gene is a zinc-finger (Cys2-Hys2) transcriptional factor considered to be an active repressor that controls the number of stamens and carpels in A. thaliana. Moreover, SUP is involved in the floral meristem termination and the development of the carpel marginal derived tissues. The main objective of this work was the functional characterization of the SUP orthologue in the model legume Medicago truncatula (MtSUP). We achieved this objective based on a reverse genetic approach, gene expression analysis, and complementation and overexpression assays. Our results show that MtSUP is the orthologous gene of SUP in M. truncatula. MtSUP shares some of the roles already described for SUP with variations. Interestingly, MtSUP controls the determinacy of the secondary inflorescence (I2) meristem and the common primordia (CP). Thus, MtSUP controls the number of flowers and petal-stamens produced by the I2 meristem and the common primordia respectively. MtSUP displays novel functions for a SUP-like gene, playing key roles in the meristems that confer developmental complexity to this angiosperm family. This work allowed to identify MtSUP, a key gene that participates in the genetic regulatory network underlying compound inflorescence and flower development in the model legume M. truncatula. / I would like to thanks the Spanish Ministry of Economy and Competitiveness for the grant (MINECO; BIO2016-75485-R) that supported this work. Special thanks to the Generalitat Valenciana for funding my doctorate with the Santiago Grisolía predoctoral scholarships / Rodas Méndez, AL. (2021). MtSUPERMAN controls the number of flowers per inflorescence and floral organs in the inner three whorls of Medicago truncatula [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/171474 / TESIS

Th. 3e cycle--Biologie et physiologie végétale--Strasbourg--Université Louis-Pasteur, 1983. / Bibliogr. p. 143-146 . Index.

‘Crimson Seedless’ (Vitis vinifera L.) is an attractive, late season, red, seedless cultivar, which is currently a very popular table grape cultivar. It is one of the most planted cultivars in South Africa and third in terms of total area of table grape vineyards in production. Mature ‘Crimson Seedless’ grapes are characterized by outstanding eating quality, good flavour, firm and crisp berries. One of the key factors affecting the yield of table grape cultivars is bud fruitfulness. Low fruitfulness can have a significant effect on the yield of table grape cultivars and ‘Crimson Seedless’ is characterized by a fruitfulness problem. Summer pruning, such as the removal of shoots after harvesting grapes, is a cultivation practice widely used by some table grape producers in the Orange River region of South Africa. The first hypothesis of this study stated that the removal of shoots after harvest will increase the transmitted PAR through the canopy, increase carbohydrate reserve levels in canes and improve bud fruitfulness of ‘Crimson Seedless’. A second hypothesis of this study stated that the cut back of all main shoots and shoots developing from spurs to the nearest lateral shoot and the removal of all unproductive shoots after berry set will result in fruitful shoots the following season. The third and final hypothesis of this study stated that the removal of shoots after harvest and berry set will improve the yield and quality of Vitis vinifera cv. ‘Crimson Seedless’. The study was conducted over three seasons (2010/11 to 2012/13) and aimed at investigating factors, including shoot removal, impacting bud fruitfulness of an 11-year-old commercial V. vinifera L. cv. ‘Crimson Seedless’ vineyard, grafted on ‘Ramsey’ (Vitis champinii) rootstocks in the Hex River Valley. The treatment design was a complete randomized design and involved five treatments, which included 33% shoot removal (S33) and 66% shoot removal (S66) after harvest, cutting of all main and lateral shoots developing from spurs to the nearest lateral shoot (LS), removal of all unproductive shoots (RSB) which was compared with the control, in which standard pruning practices were performed. The results obtained in this experiment showed that shoot removal after harvest and after berry set improves PAR transmission into the canopy, but there was no significant impact on bud fruitfulness. In addition, it was found that shoot removal reduced vegetative growth resulting in thinner canes that also led to the improvement in PAR transmission. Furthermore, bunch number per shoot in the LS treated vines was reduced when compared with S33 treated vines, illustrating that shoot removal at berry set can reduce bunch number per shoot due to defoliation after berry set. The significant decrease in bud burst in the S33 treatments compared with the control was expected due to less shoots, resulting in a reduction in cane mass during the 2010/11 season. The significant effect of LS treatments after berry set on TSS and total red pigments compared with the S66 treatments and the control, respectively, clearly indicates that shoot removal after berry set improves grape colour. The positive effect of LS treatments on colour was supported by the significant improvement in class 4 bunches, representing an improvement in quality. Although shoot removal did not have a significant effect on the bunch mass per vine of ‘Crimson Seedless’, there was a significant reduction in total bunches for export and mass of the total export bunches in the LS treatments in the 2011/12 season. A link between carbohydrate concentration in canes and bud fruitfulness was not found in this study, as S33 and S66 treatments did not have a significant effect on carbohydrate content in canes during the 2011/12 season. The question therefore arises whether the treatments applied during the growing seasons are worthwhile, because there was no significant impact on bud fruitfulness of Vitis vinifera L. cv. ‘Crimson Seedless’. This study illustrates that growers need to decide whether it is worthwhile to utilize labour for this practice and they must manage grapevines not only for the current seasons crop, but also for the next season and this can be accomplished by maintaining sufficient carbohydrates for fruitfulness and yield from season to season. / Dissertation (MInst Agrar)--University of Pretoria, 2014. / tm2015 / Plant Production and Soil Science / MInst Agrar / Unrestricted