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Despite the many interventions and trials aimed at improving coordination of health care, there is currently no accepted measurement of coordination. My professional interests and an opportunity provided by the Care Plus (Coordinated Care) Trial in the ACT led me to consider client perceptions as a potentially appropriate measure. My research question is “can coordination of health care be usefully measured through client perceptions?” ¶ I addressed this question by developing and testing an instrument to measure perceptions of coordination called the Client Perceptions of Coordination Questionnaire (CPCQ). In the thesis I describe the processes of developing the instrument, testing it through use in several studies and considering how useful such an instrument may be for health services research. In addition to the Coordinated Care Trial, I conducted two validation studies - in a chronic pain population and a general practice sample. ¶ In Part 1 of the results I demonstrate good face, content and discriminant validity, and reliability of the instrument. Psychometric analysis of the CPCQ did not support scaling, and identified areas were the instrument could be improved. Nevertheless the underlying construct of client perception of coordination as a measure is entirely new, and it is therefore worthwhile to explore its associations with other health outcome data. In Part 2 I use a single item from the CPCQ, “how often did you feel the care you received was well-coordinated?” to explore this construct. I explore the influences upon on client perceptions of coordination, and examine its associations with service utilisation and health outcome data. ¶ The construct of ‘perceived coordination’, and the capacity of the CPCQ to measure it accurately show considerable promise as measures of health care. Consistent trends were found that suggested coordination was a complex construct, and that a stronger theoretical base was needed to interpret this complexity. Theory is the first of the ‘phases’ of instrument development, and so I revisit these in order to summarise the strengths and weaknesses of the current CPCQ. Overall, the perceptions of coordination have surprisingly plausible associations with other health data, the effort to improve the instrument should be worthwhile for a wide range of health service evaluation and research.

The increasing world population is putting pressure on global food production. Agriculture must meet these growing demands by increasing crop yields. One phenomenon which prevents seedling emergence and damages crop yield is soil crusting. Understanding of soil crusting and the factors which influence it is fundamental to ensuring good crop production. An instrument which will test soil crust strength in a novel way, mimicking seedling growth, may lead to pre-emptive agricultural soil management which could increase crop production. This work details the process of design, construction and testing of an ascending penetrometer to measure soil crust strength. The full design process is discussed from concept generation and evaluation, using experimental methods and a multi-criteria decision making tool, through to final design configuration, specification, manufacture and testing. Traditionally, soil penetrometers measure soil strength by forcing a probe from the surface of the soil into the bulk soil below. To more accurately measure the direct impedance a seedling would experience a device should measure impedance from the bulk soil upwards and into the soil crust, mimicking what a growing seedling would experience. Results prove that the manufactured ascending penetrometer with a force resolution of 0.01N and displacement resolution of 0.0004mm is capable of detecting differences in soil crusts. At these resolutions and accuracy to 0.1N and 0.1mm excellent repeatability was achieved. The machine is therefore a useful and realistic tool for quantitatively comparing soil crusts in soil. It is hoped that being able to compare soil crust strength will lead to improved soil management techniques.

All animals harbor microorganisms that interact with each other and with their hosts. These microorganisms play important roles in health, disease, and defense against pathogens. The microbial communities in the intestine are particularly important in preventing colonization by pathogens; however, this defense mechanism and the means by which pathogens overcome it remain largely unknown. Moreover, while the composition of animal-associated microbial communities has been studied in great depth, the spatial and temporal dynamics of these communities has only recently begun to be explored. Here, we use a transparent model organism, larval zebrafish, to study how a human pathogen, Vibrio cholerae, invades intestinal communities. We pay particular attention to a bacterial competition mechanism, the type VI secrection system (T6SS), in this process. In vivo 3D fluorescence imaging and differential contrast imaging of transparent host tissue allow us to establish that V. cholerae can use the T6SS to modulate the intestinal mechanics of its host to displace established bacterial communities, and we demonstrate that one part of the T6SS apparatus, the actin crosslinking domain, is responsible for this function. Next, we develop an automated high-throughput light sheet fluorescence microscope to allow rapid imaging of bacterial communities and host cells in live larval zebrafish. Light sheet fluorescence microscopy (LSFM) has been limited in the past by low throughput and tedious sample preparation, and our new microscope features an integrated fluidic circuit and automated positioning and imaging to address these issues and allow faster collection of larger datasets, which will considerably expand the use of LSFM in the life sciences. This microscope could also be used for future experiments related to bacterial communities and the immune system. The overarching theme of the work in this dissertation is the use and development of advanced imaging techniques to make new biological discoveries, and the conclusions of this work point the way toward understanding pathogenic invasion, maximizing the use of LSFM in the life sciences, and gaining a better grasp of host-associated bacterial community dynamics. This dissertation includes previously published and unpublished co-authored material.

The use of stars as a radiation source for ground-based ozone remote-sensing instruments is explored and an automated prototype instrument that measures absorption due to atmospheric ozone in stellar spectra has been designed, implemented and tested. <p> This work represents the proof-of-concept development of a low-cost, low dispersion slitless imaging spectrometer that measures Chappuis-band absorption in stellar spectra. The work presented here progresses from the initial concept to a functional calibrated prototype that is capable of nightly automated observations of visible-band spectra from mid-magnitude stars. The design and calibration of the prototype and subsequent data collection and analysis are presented. <p>A slitless imaging spectrometer has been developed and integrated with a commercial self-pointing telescope and an astronomical imager. A relative intensity calibration and the development of a dynamic wavelength calibration scheme, necessitated by the slitless nature of the instrument, is presented. The calibrated prototype has been used to collect several data sets of stellar spectra, and it is shown that the instrument can detect Chappuis absorption in stellar spectra. Several issues with both the concept and design that must be addressed in further development of the prototype are identified.

The use of stars as a radiation source for ground-based ozone remote-sensing instruments is explored and an automated prototype instrument that measures absorption due to atmospheric ozone in stellar spectra has been designed, implemented and tested. <p> This work represents the proof-of-concept development of a low-cost, low dispersion slitless imaging spectrometer that measures Chappuis-band absorption in stellar spectra. The work presented here progresses from the initial concept to a functional calibrated prototype that is capable of nightly automated observations of visible-band spectra from mid-magnitude stars. The design and calibration of the prototype and subsequent data collection and analysis are presented. <p>A slitless imaging spectrometer has been developed and integrated with a commercial self-pointing telescope and an astronomical imager. A relative intensity calibration and the development of a dynamic wavelength calibration scheme, necessitated by the slitless nature of the instrument, is presented. The calibrated prototype has been used to collect several data sets of stellar spectra, and it is shown that the instrument can detect Chappuis absorption in stellar spectra. Several issues with both the concept and design that must be addressed in further development of the prototype are identified.

An ultraviolet instrument compatible with a CubeSat form factor is currently being developed at Virginia Tech for the purpose of measuring nitric oxide in the polar night through the stellar occultation technique. This instrument will allow the investigation of how the Sun and Earth systems are related via energetic particle precipitation in the auroral regions. The work performed in this thesis supports the instrument design and requirements development by modelling the stellar occultation geometry to identify orbit parameters and target stars that could yield nitric oxide measurements during the polar winter at consistent latitudes, to best observe the build-up and fall-off of nitric oxide. The orbit study was accomplished through the development of an open-source tool in MATLAB, the Stellar Occultation Mission Planner. The results of this analysis were used to model the instrument performance and identify the required narrowband filter parameters to meet science requirements. Additional studies were performed to explore system performance for a future flight opportunity. / Master of Science / A small, light weight instrument is being designed at Virginia Tech to allow for nitric oxide in the atmosphere to be measured during the long polar nights that occur during winter in the Arctic and Antarctic regions. This instrument will allow scientists to explore how the Sun and Earth interact through space weather at high latitudes. This will be accomplished by using star light to probe the atmosphere while the instrument is onboard a small spacecraft called a CubeSat. The work performed in this thesis simulated the spacecraft orbit to determine which stars yielded the best measurements over the course of the polar night. Using these results, the instrument performance was simulated to inform the design of a filter for the instrument. Additional studies were performed to support the design of a future mission to fly the instrument in space.

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This study examines the development of German pipe organ culture and French classical pipe organ culture from a single common origin in the Duchy of Brabant, during 1450-1850, with respect to select major attributes of organ construction, organ music and organ technique. The respective music of Dieterich Buxtehude (1637-1707), Praeludium in G Minor, BuxWV 149, for the North German contrapuntalists, and of Câesar Franck (1822- 1890), Choral No. 3 in A Minor, for the classical French symphonic tradition, is highlighted and appended with suggested technique for each work. / by Margaret R. Miller. / Thesis (M.A.)--Florida Atlantic University, 2010. / Includes bibliography. / Electronic reproduction. Boca Raton, Fla., 2010. Mode of access: World Wide Web.