This study evaluates the adoption and impacts of Integrated Pest Management (IPM) adoption for rice in Cambodia. Extent of adoption and characteristics of adopters are discovered. Farmers are considered high adopters of IPM if they used two non-pesticide or minimal-pesticide practices to control rice insect, disease, weed, or rodent pests in the last twelve months; farmers are considered low adopters if they used one practice; farmers are considered non-adopters if they used zero practices. IPM practices include pest-resistant variety; stale seedbed (sequential harrowing or harrowing followed by a non-selective herbicide); apply Trichoderma on seeds or seedlings, no insecticide spray for the first 40 days; apply bio-pesticides such as neem, Bt, and metarhizium, and Beauvaria; Sarcocystis bait for rodents; hand weeding at recommended growth stage; and/or another practice specified by the farmer. Out of 394 farmers surveyed, 40 (10.15%) were found to be high adopters, 228 (57.86%) were found to be low adopters, and 126 (31.97%) were found to be non-adopters of IPM. IPM practices currently include mostly hand-weeding and no spray for 40 days; few other practices were adopted. Our study reveals a need for broader education on rice IPM throughout Cambodia. The high frequency of pesticide applications among rice farmers, the finding that adoption of IPM was not found to have a meaningful influence on the number of pesticide applications, and the finding that less than one-quarter of farmers in our study have received training on IPM reveal the need for increased knowledge of IPM in Cambodia, and the need for future education on IPM to focus on reducing pesticide use. / Master of Science

Doctor of Philosophy / Department of Entomology / Franklin Arthur / James R. Nechols / The Indianmeal moth, Plodia interpunctella Hubner, is capable of infesting a number of different commodities including a wide variety of grains, nuts and finished stored products. Therefore, control of the Indianmeal moth is especially needed in areas where food is being stored for human consumption. Increased concerns of consumers and producers regarding the impact of conventional insecticides on the environment and on human health has prompted scientists and the agricultural chemical industry to search for insecticides that do not affect mammalian nervous systems and have limited effects on non-target organisms. One group of insecticides with reduced risks is insect growth regulators (IGRs), which are substances that mimic insect hormones essential to normal development and reproduction. Currently methoprene, a juvenile hormone analog, is labeled for direct application to stored grains, as well as a contact insecticide and as an aerosol application inside mills, warehouses, and indoor food storage facilities. Surface treatments and aerosol space applications can be effective ways to treat the interior surfaces and storage areas of warehouses and food processing facilities. There is little recent research with large-scale aerosol applications in storage sites; furthermore, there are no published references in the scientific literature regarding efficacy of using methoprene alone in aerosol form. Therefore, the purpose of this research was to evaluate the use of surface and aerosol applications of methoprene on finished stored-product packaging materials and facilities for the control of P. interpunctella. Results of this research showed that while methoprene has good residual activity, and efficacy is unaffected by temperature, surface applications of methoprene on packaging materials is not as effective for control of P. interpunctella as aerosol applications of methoprene. Aerosol methoprene is highly effective alone and in combination with conventional chemicals for control of eggs and wandering-phase larvae. Simulations with a population growth model make it possible to estimate impact of insecticide treatments at different temperatures and application times on populations of P. interpunctella. Aerosol treatments are also economically viable as part of an overall integrated pest management program.

Orientador: Daniel Júnior de Andrade / Resumo: O ácaro-da-ferrugem-dos-citros Phyllocoptruta oleivora é uma das principais pragas dos citros no Brasil. Os problemas atribuídos a P. oleivora têm se intensificado e suspeita-se que os danos estejam relacionados a uma nova espécie de eriofiídeo descrita recentemente, o ácaro-marrom-dos-citros Tegolophus brunneus. No entanto, não há estudos com essa espécie e informações sobre sua distribuição, características bioecológicas, danos, suscetibilidade a acaricidas e inimigos naturais associados são ausentes. O objetivo principal do projeto foi estudar a distribuição de T. brunneus na principal região citrícola brasileira e sobre as principais espécies e variedades cítricas. Além disso, estudou-se a biologia de T. brunneus em laboratório, determinou-se a suscetibilidade desse ácaro aos principais acaricidas, caracterizando seus danos em plantas cítricas, bem como o potencial de predação das principais espécies de predadores associadas ao ácaro e seu potencial para o uso no controle biológico. As coletas realizadas em vários municípios do estado São Paulo e Triângulo Mineiro demonstraram que T. brunneus infestou apenas lima ácida ‘Tahiti’, enquanto P. oleivora infestou todas as outras espécies e variedades cítricas. O ácaro completa o desenvolvimento em 7 dias, com período de incubação de 3 dias, duração de larva de 2,1 dias e de ninfa 2,8 dias e as fêmeas apresentam fecundidade de 8,5 ovos a 25°C. Os danos da espécie caracterizam-se pelo prateamento dos frutos e formação de manchas... (Resumo completo, clicar acesso eletrônico abaixo) / Doutor

Delia radicum L. (Diptera: Anthomyiidae), the cabbage root fly, is a specialist root-feeding insect pest of Brassica crops. The impending withdrawal of chlorpyrifos, one of the main pesticides used against D. radicum, opens new opportunities to research alternative pest management strategies. Manipulating host plant location cues to influence D. radicum adult and larval behaviour, along with induced plant defence responses, offer potential integrative crop protection solutions (Chapter 1). This thesis aimed to identify the semiochemistry underpinning D. radicum larval host plant location, and to investigate whether plant defence induction treatments (methyl jasmonate [MeJA], D-Fructose) and a herbivore induced volatile (dimethyl disulfide [DMDS]) affect D. radicum larval performance and adult oviposition preference. In choice-test bioassays, larvae were inconsistent in their responses to root exudates collected from Brassica host plants (Chapter 2). A combined bioassay and EthoVision® video-tracking approach was developed to record and analyse larval movements in response to volatiles emitted from host and non-host plant roots (Chapter 3). Larvae were significantly attracted to host plant root volatiles. Olfactory stimuli from roots of the non-host plant onion (Allium cepa L. ‘Ailsa Craig’), which share overlapping, yet distinctive volatile profiles to that of Brassica plants, also elicited positive taxis. By analysing the volatile metabolome of broccoli (B. oleracea L. convar. botrytis L. Alef. var. cymosa Duchesne ‘Parthenon’) and onion roots using solid phase micro extraction-gas chromatography-mass spectrometry (SPME-GCMS), a suite of candidate volatile orientation cues were identified. A SPME-based method was developed to non-invasively collect root volatiles in situ from glasshouse- and field-grown broccoli plants pre- and post-D. radicum infestation (Chapters 4, 5 and 6). GC-MS analyses revealed that sulfur compounds, showing characteristic temporal emission patterns, were the principal volatiles released by roots in response to damage. This new method, which has potential for wide application in chemical ecology research, allows the study of volatiles in the soil in situ that are critical for interactions between trophic levels. In EthoVision® bioassays, a major volatile constituent of broccoli roots, DMDS, was attractive to larvae, but toxic at the highest dose tested (Chapter 3). Glasshouse and on-farm experiments using broccoli were conducted to evaluate the efficacy of MeJA, D-Fructose and DMDS against D. radicum compared to commercially available crop protection products (chlorpyrifos [Dursban® WG], spinosad [Tracer®], Steinernema feltiae Filipjev [Nematoda: Steinernematidae] [Entonem] and garlic granules [ECOguard®]). MeJA and garlic reduced larval performance under glasshouse conditions whereas D-Fructose and DMDS did not at the concentrations tested (Chapter 7). In field studies, MeJA combined with reduced rate chlorpyrifos, spinosad, and S. feltiae all showed partial efficacy for controlling D. radicum larvae. Inherent field site, weather and D. radicum population density variability highlighted that glasshouse results cannot always be reproduced in more complex field environments (Chapter 8). Further research is needed into formulation, mode of application and timing to improve efficacy of promising treatments that may help in future integrated pest management (IPM) for this key pest in the absence of existing pesticides.

11 pp. / This document provides a general overview of bat biology and behavior with emphasis on urban environments, use of integrated pest management (IPM) techniques that are in keeping with bat conservation guidelines, and disease awareness and prevention efforts.

31 pp. / Many pests encountered in homes and structures can be prevented by using simple techniques collectively known as “pest-proofing”. If done correctly, pest-proofing your home saves you money by reducing pest management costs, and more importantly, reduces potential pesticide exposure. This publication describes general indoor and outdoor pest-proofing measures and some of the major pests encountered in and around homes and structures.

Doctor of Philosophy / Department of Grain Science and Industry / X. Susan Sun / With continued population growth, more food production will be required with lower resource inputs. A significant drain on resources is post-harvest loss due to insects, which results in loss of product, quality and market access, and increased grain spoilage. Aeration and fumigation are key tools to control insect growth in stored grains and grain foods. The implementation of aeration strategies in Australia is made difficult by the warm subtropical climate, meanwhile the success of fumigation is being threatened by the spread of insect resistance to the fumigant phosphine. This dissertation project seeks to improve the understanding of aeration and fumigation by modifying the Maier-Lawrence (M-L) 3D ecosystem model by adding insect growth equations and quantifying fumigant loss from sealed bulk grain silos. The improved model was used to examine aeration under Australian conditions, validate its capability to accurately describe fumigant concentrations during silo fumigation, determine the extent to which fumigations are influenced by operational variables and environmental conditions, and validate its capability to describe fumigant concentrations post-fumigation in order to investigate the time needed to clear a grain storage silo of fumigant in order to assure worker safety. Six aeration strategies were evaluated under Australian conditions. Of these strategies, two were found to be effective in lowering temperatures, i.e., fans were turned on when ambient temperature was less than 20oC, and less than internal grain temperature. The strategy based on temperature differential was the most effective because it cooled the interior of the grain mass with the least amount of energy (using the fewest fan run hours, and reaching 15oC an average of 11 days after than the fastest strategy). Using a 0oC temperature differential was the most effective strategy in terms of reducing insect growth. The expanded (M-L-P) model was then validated based on experimental fumigant concentrations. The model was effective in reproducing average fumigant concentrations and fumigant trends vertically through the grain mass, but was not able to reproduce lateral fumigant variations. Verifications of the model with two different periods of phosphine release (i.e., 24h and 30h) were tested. Based on a 24h phosphine release period the predicted Ct-product differed from the experimental value by 0.9%. A 30h release period predicted a Ct-product that differed by 4.3% from the experimental value but it was more accurate during the times of highest concentration. Increases in leakage reduced fumigant concentrations, but the size of the effect decreased as leakage grew. Increasing temperature and wind speed in the model led to decreased phosphine concentrations, with temperature changes having a more significant impact overall than wind speed changes for the conditions investigated. Decreasing silo surface area to volume ratio dampened the impact of changing weather conditions on phosphine concentrations. A fumigant venting experiment was conducted in a silo at Lake Grace, Australia, to investigate full scale desorption. Two equations estimating fumigant desorption were tested, with an average of 65.5% and -86.3% error. The length of venting periods was simulated to quantify hours needed to mitigate hazardous conditions. For the scenarios investigated 10 to 24 h of venting were needed to reduce residual fumigant concentration below 0.3 ppm depending on simulation assumptions.

The parasitic weeds belonging to the genus Striga are among the world's most tenacious, prolific and destructive agricultural pests. Crop loss estimates due to Striga infestations can reach 100 percent. Furthermore, the weeds' affinity for low-fertility soils and low rainfall means that those farming the most marginal lands are most severely affected. Nonetheless, subsistence farmer have yet to adopt seemingly beneficial control practices to any appreciable degree. This paper develops a bio-economic model capable of identifying: (1) affordable, effective Striga control practices consistent with the resource constraints of subsistence farmers; and (2) barriers to the adoption of those practices. The model is comprised of two components: a biological component modeling Striga population dynamics, and an economic component representing the production opportunity set, resource constraints, and price parameters farmers face. The model is applied to two zones in Northwestern Mali, Sirakorola and Mourdiah, and solved using non-linear, dynamic programming. Data collected by the USAID IPM-CRSP/Mali project are used to specify the economic parameters of the model. A new technique for estimating the lower bound of a farmer's production planning horizon is also developed and employed in the application of the model to Sirakorola and Mourdiah. The results of several model scenarios indicate that the availability of information regarding the efficacy of Striga control practices is a primary barrier to their adoption by subsistence farmers. The movement of Striga seed between fields, however, is of limited importance. The "optimal control practices" identified by the model depend on the size and demographic composition of the production unit (UP), the zone in which the UP is located, and the cash budget available to the UP. At low budget levels, the model suggests planting millet without fertilizer at a high density in Sirakorola and a low density in Mourdiah. At high budget levels, the model suggests planting millet at a high density in both zones while applying urea. The benefits of adopting the optimal set of practices are presented in both nutritional and financial terms, and can reach as much as a ten-fold increase in the nutritional content of and financial returns to a harvest. / Ph. D.

Tuta absoluta, a member of the moth family, causes devastating yield loss to tomato farmers around the world. Its recent migration into the tomato fields of Nepal puts tomato farmers at a high risk of yield loss. In response, chemical pesticide use by Nepali farmers is increasing. Integrated pest management (IPM) practices have been implemented in hopes of reducing the frequency of chemical pesticide use while controlling yield risks. This study examines the extent and determinants of Tuta absoluta IPM adoption and its effect on the frequency of pesticide use for Nepali tomato farmers. Primary data was collected from four-hundred and one households in four districts throughout Nepal. Two levels of IPM practices were assessed, simple and complex, based on the need for additional knowledge and tools. An instrumental variable probit analysis was used to analyze the determinants of IPM adoption. Household distance to nearest agricultural extension office was a significant factor decreasing the likelihood of the adoption of complex practices. Amount of land dedicated to tomato production, membership status of the primary decision maker, IPM training regarding Tuta absoluta practices and severity of Tuta absoluta were found to increase the likelihood of the adoption of complex practices. In order to analyze pesticide use, a simple linear regression was used. Primary decision maker's age, gender, and education level were significant determinates to decrease the amount of expenditures spent on chemical pesticides to control for Tuta absoluta. IPM adoption level, amount of land dedicated to tomato production and severity of Tuta absoluta damage were significant determinates to increase the amount of expenditures spent on chemical pesticides to control for Tuta absoluta. / Master of Science / Tuta absoluta, a member of the moth family, causes devastating yield loss to tomato farmers around the world. Its recent migration into the tomato fields of Nepal puts tomato farmers at a high risk of yield loss. In response, chemical pesticide use by Nepali farmers is increasing. Integrated pest management (IPM) practices have been implemented in hopes of reducing the frequency of chemical pesticide use while controlling yield risks. This study examines the extent and determinants of Tuta absoluta IPM adoption and its effect on the frequency of pesticide use for Nepali tomato farmers. Data was collected from four-hundred and one households in four districts throughout Nepal. Different economic tools were used to conduct the analyses. Results show the farther a household is to the near agricultural extension office, the less likely a household is to adopt complex practices. An increased amount of land dedicated to tomato production, the primary decision maker being a member of a marketing or community organization, the primary decision maker having attended IPM training regarding Tuta absoluta practices and the greater the severity of Tuta absoluta in the household tomato fields, the more likely a household is to adopt complex practices. Contrary to one of the goals of IPM practices, this study shows complex adopters use more chemical pesticides than simple adopters. Results this study shows a need for further education of the relationship between IPM practices and the use of chemical pesticides.

The majority of Nepal's population relies on agriculture, so invasive and native pests' ability to reduce farmers' crop yields is a significant concern. To protect farm households' food security and livelihoods, it is imperative to find effective pest management products and practices. Integrated pest management (IPM) is an arguably cheaper and less harmful alternative to conventional synthetic pesticides and is a way of managing and preventing agricultural pests using different levels of control methods (e.g., biological, cultural, and chemical) that have minimal adverse environmental and human health impacts. This study provides information on the extent of IPM practices by Nepali vegetable farmers, adds to the understanding of factors that influence the IPM adoption decision, and compares the economic benefits and performance of IPM to other conventional pest management practices. Our survey of 346 vegetable farmers in four districts throughout Nepal provides the primary data we use in our analysis. We distinguish practices into two categories: simple IPM practices that are commonly used and require limited knowledge and complex practices that typically require more knowledge and conscious use of IPM itself. We use a probit model to determine the factors that significantly affect the decision to adopt complex IPM practices. Our results find two explanatory variables that consistently affect complex IPM adoption: gender and IPM training. We compare the costs and benefits of using IPM to other conventional pest management practices by analyzing results from experimental field trials conducted in Nepal's Banke and Surkhet districts. Using an economic surplus approach, we estimate the market-level benefits of using IPM practices for three vegetables in Banke and four vegetables in Surkhet. The results predict cumulative IPM benefits of $1.06 to $1.44 million across the two districts. / Master of Science / The majority of Nepal's population relies on agriculture, so invasive and native pests' ability to reduce farmers' crop yields is a significant concern. To protect farm households' food security and livelihoods, it is imperative to find effective pest management products and practices. Integrated pest management (IPM) is an arguably cheaper and less harmful alternative to conventional synthetic pesticides and is a way of managing and preventing agricultural pests using different levels of control methods (e.g., crop rotation, weeding, pheromones to disrupt mating) that have minimal adverse environmental and human health impacts. This study provides information on the extent of IPM practices by Nepali vegetable farmers, adds to the understanding of factors that influence the IPM adoption decision, and compares the economic benefits and performance of IPM to conventional synthetic pesticides. Our survey of 346 vegetable farmers in four districts throughout Nepal provides the primary data we use in our analysis. We distinguish practices into two categories: simple IPM practices that are commonly used and require limited knowledge and complex practices that typically require more knowledge and conscious use of IPM itself. Various statistical methods are used and find that gender and IPM training consistently affect the decision to adopt complex IPM practices. Compared to female Nepali vegetable farmers, males are more likely to adopt complex practices. In addition, attending an IPM training event increases the likelihood of farmers using complex IPM practices. We use data from field trials of farmers in Surkhet and Banke, IPM adoption rates from the survey, and information on Nepal's vegetable market to calculate the economic benefits of farmers using complex IPM practices for tomato, cauliflower, onion, and cucumber production. The results predict cumulative IPM benefits of $1.06 to $1.44 million to vegetable consumers and producers across the two districts.