Thesis (M.S.)--University of North Carolina at Wilmington, 2003. / Includes bibliographical references (leaves : 39-42).

Thesis (Ph. D.)--Stanford University, 2008. / Submitted to the Department of Biology. Copyright by the author. Includes bibliographical references (p. 183-205). Also available online.

The intertidal flora and fauna of S.E. Australia (Robe, South Australia to S. New South Wales, including Tasmania) is described in terms of its horizontal and vertical distribution. A detailed account of intertidal zonation, on the central Victorian coast and Bass Strait islands, is included. Environmental features are discussed and sea temperature data for Pt. Lonsdale is presented. / Analysis of the marine flora in S.E. Australia suggests that the concept of separate biogeographic provinces in this region is invalid and the flora is best interpreted as a continuum.

With the anticipated effects of climate change due to global warming, there is concern over how animals, especially ectotherms, will respond to or tolerate extreme and fluctuating environmental temperature stress. Littorinid snails are intertidal ectotherms that live high on the shore where they experience both extreme and variable conditions of temperature and desiccation stress, and are believed to live close to their tolerance limits. This study investigated the thermal biology of littorinid snails of the genera Afrolittorina, Echinolittorina and Littoraria from temperate, subtropical and tropical regions in South Africa and Brunei Darussalam using thermal tolerance, heart function, and proteome approaches. The effects of conditions, such as rate of change in temperature, acclimation, heat shock, season and starvation were also tested. In addition, the evolutionary relationships and genetic diversity between and within the South African Afrolittorina spp. were investigated using mitochondrial and nuclear markers. Genetic results confirmed that these are two distinct species, with the brown to black A. knysnaensis predominant in the cool-temperate region of South Africa and the pale blue-grey A. africana in the subtropical region. There was low genetic variation and differentiation within each species, suggesting high gene flow among populations as a result of the effects of ocean currents on the dispersal of their planktotrophic larvae. Tests using exposure to high temperatures revealed differences in the thermal tolerances, heart performance and protein profiles of species from different latitudes, regions and zones on the shore. Thermal tolerance conformed to expectations, with clear, statistically significant trends from high tolerance in subtropical species to lower tolerance in temperate species. However, for Afrolittorina spp., there were no significant differences in the thermal tolerances of conspecifics from different regions, though there was a significant difference in thermal tolerance between juveniles and adults. Overall, adults of all species showed higher thermal tolerances than juveniles. Although lethal temperatures for these species were higher in summer than winter, laboratory acclimation had no effect on heat coma temperatures. All species showed some regulation of heart rate, with a degree of independence of heart rate from temperature across mid-range temperatures. The tropical species showed quick induction and good regulation of heart rate followed by the subtropical and temperate species, which displayed mixed responses including regulation, partial regulation and lack of regulation. Overall, tropical Echinolittorina spp. showed good regulation, while the subtropical E. natalensis and Littoraria glabrata exhibited a mixture of partial regulation and regulation. The subtropical/temperate Afrolittorina spp. showed high individual variability, some animals exhibiting regulation, while others did not. These effects seem to be largely phylogenetically determined as there were no differences in the heart rate responses of Afrolittorina spp. from different regions. The temperatures at which heart rate became independent of temperature (thermoneutral zone) were within the range experienced under natural conditions. In addition, there were differences in Arrhenius breakpoint and endpoint temperatures, showing a trend from higher in tropical animals to lower for temperate animals. Conditions such as acclimation, heat shock and starvation had little or no effect on heart performance. However, a slow increase in temperature induced good regulation of heart rate with noticeable shifts of breakpoints and endpoints for Afrolittorina spp. Lastly, there were differences in the proteome responses between and within Afrolittorina spp. as a function of species, size and treatment. Although both large and small A. knysnaensis had a greater number of protein spots in their proteome than A. africana (though the difference was not significant), the later showed significantly higher differential expression of certain proteins following heat stress. In addition, juveniles of both species displayed greater numbers of protein spots in their proteome than adults. The results indicate a difference in the physiological and biochemical responses (i.e. adaptations) of these snails to temperature, and this seems to relate to differences in biogeography, phylogeny, species identity and ecology. The ability to regulate heart rate is phylogenetically determined, while thresholds and lethal limits correspond to biogeography and species ecology. The proteome seems to correspond to species ecology. The results also indicate that these littorinids can tolerate high temperature stress and in this respect they are well suited to life in the intertidal zones or habitats where temperature and other stresses or conditions are extreme and can change abruptly. However, the limited ability of these snails to acclimate to different temperatures suggests that they are already living close to their tolerance limits with small safety margins or narrow thermal windows and so may be vulnerable to small rises in substratum temperature and/or solar radiation.

Thesis (Ph. D.)--Joint Program in Oceanography/Applied Ocean Science and Engineering (Massachusetts Institute of Technology, Dept. of Biology; and the Woods Hole Oceanographic Institution), 2005. / Includes bibliographical references. / The geographic distribution and dynamics of coastal benthic populations are shaped by physical - biological interactions affecting larval dispersal and the demography of juvenile and adult individuals. This thesis focused on nearshore patterns of larval distribution and regional patterns in demography of intertidal barnacles in Southern and Baja California. Horizontal and vertical distributions, and the mortality rates of larvae, were assessed from short term (i.e. days) small- scale observations (0.1-1 km) in nearshore waters. Observations on spatial variability of adult barnacle demography were gathered over 1.5 years at scales of hundreds of kilometers. Stage-specific horizontal distributions and nearshore current measurements suggested that larvae of Balanus g-landula and Chthamalus spp. may experience limited dispersal. High mortality rates could further limit travel distances and the exchange of individuals among disjunct populations. Data on vertical distributions indicated that nauplii and cyprids of Balanus nubilus and Pollicipes polymerus occur at different depths. Nauplii remained near the surface at all times, whereas cyprids occurred in the bottom half of the water column. / (cont.) Such distributions, combined with vertical variability in horizontal flows, might cause the observed horizontal segregation of nauplii and cyprids. Differences in survival, growth rate, size structure, and per capita fertility of adult Balanus glandula were observed between Dana Point (Southern California) and Punta Baja (Baja California), a site located near the species' southern limit of distribution. Effects of spatial differences in demography on population persistence were assessed with a stage-structured matrix model. Model analyses indicated that the Punta Baja population is more susceptible to environmental stochasticity and more prone to local extinction than populations located further north. This thesis emphasizes the importance of characterizing factors that affect the dynamics of benthic populations at multiple spatial-temporal scales, and the usefulness of small scale high- frequency observations of nearshore phenomena, especially in relation with the dispersal of larvae. / by Fabián J. Tapia. / Ph.D.

Most climate change predictions focus on the response of individual species to changing local conditions and ignore species interactions, largely due to the lack of a sound theoretical foundation for how interactions are expected to change with climate and how to incorporate them into climate change models. Much of the variability in species interaction strengths may be governed by fundamental constraints on physiological rates, possibly providing a framework for including species interactions into climate change models. Metabolic rates, ingestion rates and many other physiological rates are relatively predictable from body size and body temperature due to constraints imposed by the physical and chemical laws that govern fluid dynamics and the kinetics of biochemical reaction times. My dissertation assesses the usefulness of this framework by exploring the community-level consequences of physiological constraints. In Chapter 2, I incorporated temperature and body size scaling into the biological rate parameters of a series of realistically structured trophic network models. The relative magnitude of the temperature scaling parameters affecting consumer energetic costs (metabolic rates) and energetic gains (ingestion rates) determined how consumer energetic efficiency changed with temperature. I systematically changed consumer energetic efficiency and examined the sensitivity of network stability and species persistence to various temperatures. I found that a species' probability of extinction depended primarily on the effects of organismal physiology (body size and energetic efficiency with respect to temperature) and secondarily on the effects of local food web structure (trophic level and consumer generality). This suggests that physiology is highly influential on the structure and dynamics of ecological communities. If consumer energetic efficiency declined as temperature increased, that is, species did best at lower temperatures, then the simulated networks had greater stability at lower temperatures. The opposite scenario resulted in greater stability at higher temperatures. Thus, much of the community-level response depends on what species energetic efficiencies at the organismal-level really are, which formed the research question for Chapter 3: How does consumer energetic efficiency change with temperature? Existing evidence is scarce but suggestive of decreasing consumer energetic efficiency with increasing temperature. I tested this hypothesis on seven rocky intertidal invertebrate species by measuring the relative temperature scaling of their metabolic and ingestion rates as well as consumer interaction strength under lab conditions. Energetic efficiencies of these rocky intertidal invertebrates declined and species interaction strengths tended to increase with temperature. Thus, in the rocky intertidal, the mechanistic effect of temperature would be to lower community stability at higher temperatures. Chapter 4 tests if the mechanistic effects of temperature on ingestion rates and species interaction strengths seen in the lab are apparent under field conditions. Bruce Menge and I related bio-mimetic estimates of body temperatures to estimates of per capita mussel ingestion rates and species interaction strengths by the ochre sea star Pisaster ochraceus, a keystone predator of the rocky intertidal. We found a strong, positive effect of body temperature on both per capita ingestion rates and interaction strengths. However, the effects of season and the unique way in which P. ochraceus regulates body temperatures were also apparent, leaving room for adaptation and acclimation to partially compensate for the mechanistic constraint of body temperature. Community structure of the rocky intertidal is associated with environmental forcing due to upwelling, which delivers cold, nutrient rich water to the nearshore environment. As upwelling is driven by large-scale atmospheric pressure gradients, climate change has the potential to affect a wide range of significant ecological processes through changes in water temperature. In Chapter 5, my coauthors and I identified long-term trends in the phenology of upwelling events that are consistent with climate change predictions: upwelling events are becoming stronger and longer. As expected, longer upwelling events were related to lower average water temperatures in the rocky intertidal. Furthermore, recruitment rates of barnacles and mussels were associated with the phenology of upwelling events. Thus climate change is altering the mode and the tempo of environmental forcing in nearshore ecosystems, with ramifications for community structure and function. Ongoing, long-term changes in environmental forcing in rocky intertidal ecosystems provide an opportunity to understand how temperature shapes community structure and the ramifications of climate change. My dissertation research demonstrates that the effect of temperature on organismal performance is an important force structuring ecological communities and has potential as a tractable framework for predicting the community level effects of climate change. / Graduation date: 2013 / Access restricted to the OSU Community, at author's request, from Nov. 20, 2012 - Nov. 20, 2014

Thesis (MSc (Botany and Zoology))--University of Stellenbosch, 2007. / The ability of various molluscan species to accumulate toxicants such as cadmium from natural waters in quantities that are many orders of magnitude higher than background levels are well-known. This phenomenon of bioaccumulation might cause certain stress responses in these organisms at the cellular level, which can be measured using biomarkers. A biomarker response test known as the neutral red retention assay was employed in this study to measure responses in four intertidal species. Specimens of Cymbula oculus (Born), Scutellastra longicosta (Lamarck), Cymbula granatina (Linnaeus) and Scutellastra granularis (Linnaeus) were collected at two localities on the coast of False Bay, South Africa. Laboratory exposures in static flow tanks at three different concentrations i.e. 0.8, 1 and 1.2 mg/L of CdCl2 were done respectively for each species over a three day exposure period i.e. each exposure concentration had an exposure period of 24, 48 and 72 hours. After every 24 hour exposure period the lysosomal membrane integrity was determined using the neutral red retention method to establish which species is the most sensitive to Cd. Both control and exposure groups for all species showed a decrease in retention times with an increase in Cd concentration over the exposure period. This decrease was particularly prominent at the highest exposure concentration after 72 hours. At 0.8 and 1.2 mg/L CdCl2 exposures an indirectly proportional relationship between neutral red retention time and heavy metal concentration was prominent in C. oculus, indicating a dose related response. In all species there was a moderate increase in heavy metal concentration over the 72 hour exposure period. EC50 values indicated that S. granularis and C. granatina had a “high” sensitivity to Cd contamination, while C. oculus had “medium” sensitivity and S. longicosta “low” sensitivity to Cd contamination. The sensitivity data obtained from the analysis of the experimental species in this study may contribute to the eventual establishment of a species sensitivity distribution model (SSD).

ix, 119 leaves : ill., maps ; 28 cm Notes Typescript Thesis (Ph. D.)--University of Oregon, 1985 Includes vita and abstract Bibliography: leaves 108-119 Another copy on microfilm is located in Archives

Thesis (PhD) -- Stellenbosch University, 2004. / ENGLISH ABSTRACT:Heavy metals are persistent environmental contaminants whose sources of inputs into the environment are both natural and anthropogenic. The levels of heavy metals (cadmium, copper, nickel, lead and zinc) in the False Bay intertidal zone were measured in the water, sediments and invertebrate species between August 2000 and August 2001. The results of the water and sediment analyses revealed that most pollution was associated with the northern shore of the bay between Strand and Muizenberg, where the most populated and industrial catchments occur. Significant spatial variations occurred, indicating the presence of localised contamination, while seasonal variations may be related to changes in precipitation and runoff at different times of the year. The concentrations of cadmium, nickel and lead were occasionally higher than the levels recommended by the South African Water Quality Standards. The possible sources of pollution at the different sites are also discussed. The concentrations of the five metals in the different invertebrate species (Oxystele tigrina, 0. sinensis, Choromytilus meridionalis, Patella oculus, Patiriella exigua and Tetraclita serrata) also revealed significant seasonal and spatial variations, with both the soft tissues and shells accumulating heavy metals. The barnacle T serrata from Rooiels had the highest cadmium concentration (70.67 J.lg/g dry weight), which may be related to historic pollution inputs from the military activities which took place at a weapons testing site at this site between 1987 and 1994, although no evidence was found to confirm this. The periwinkle 0. tigrina from Strand had the highest copper concentration (70.25 J.lg/g) while the limpet P. oculus from the same site had the highest nickel concentration (35.75 J.lg/g). The shells of the mussel C. meridionalis from Muizenberg had the highest concentration of lead (25.75 J.lg/g). Since cadmium occurs as a constituent of phosphate fertilisers used widely in the False Bay catchments, the effects of cadmium exposure on the different species were investigated during 14-day laboratory exposures to 200 and 400 J.lg/LCdCh. The results revealed a general pattern of tissue metal increase in the exposed organisms, followed by slight reductions after decontamination in clean seawater. The viscera and kidneys of C. meridionalis accumulated most of the dissolved cadmium. The shells of the mussels also accumulated cadmium, indicating the possible use of shells as a detoxification matrix. / AFRIKAANSE OPSOMMING:Swaarmetale is persisterende omgewingskontaminante waarvan die insetbronne beide natuurlik of van menslike oorsprong kan wees. Die kontaminasievlakke van swaarmetale (kadmium, koper, nikkel, lood en sink) in die Valsbaai tussengetysone is in die water, sedimente en invertebraatspesies bepaal vanaf Augustus 2000 tot Augustus 2001. Voorlopige resultate van die water- en sedimentontledings het getoon dat die meeste besoedeling by die noordelikke oewer van die baai voorgekom het tussen Strand and Muizenberg, waar die mees digbewoonde en ge-industrialiseerde opvangsgebiede is. Betekenisvolle ruimtelike en seisoenale variasie het in die konsentrasies van swaarmetale voorgekom, met die ruimtelike variasie wat moontlik gelokaliseerde kontaminasie aandui terwyl die seisoenale variasies weer verband mag hou met veranderings in die neerslag en afloop gedurende verskillende tye van die jaar. Die konsentrasie van kadmium, nikkel en lood was somtyds hoer as die vlakke wat deur die Suid-Afrikaanse Waterkwaliteitsstandaarde voorgestel word. Die moontlike bronne van besoedeling in die verskillende areas is ook in bespreking genoem. Die konsentrasies van die vyf swaametale in die verskillende invertebraatspesies (Oxystele tigrina, 0. sinensis, Choromytilus meridionalis, Patella oculus, Patiriella exigua and Tetraclita serrata) het ook seisoenale en ruimtelike variasies vertoon, die swaarmetale het in die sagte weefsel en skulpe van die invertebrate geakkumuleer. Die hoogste gemiddelde konsentrasie van kadmium (70.67 ).lg/g droe massa) is in die heel-liggaam monsters van die eendemossel T serrata gemeet wat by Rooiels versamel is. Die vlakke mag verband hou met die oprigting en aktiwiteite van die wapentoetsingsaanleg in die opvanggebied van die Rooiels lokaliteit tussen 1987 en 1994, maar geen bewyse daarvan is gevind nie. Die tolletjie, 0. tigrina wat in die 10kaliteit by Strand versamel is het die hoogste gemiddelde konsentrasie koper gehad 70.25 pig droe massa), terwyl die klipmossel P. oculus by dieselfde versamelpunt die hoogste konsentrasie nikkel (35.75 ).lg/gdroe massa) gehad het. Eksperimentele studies is ook uitgevoer op vier invertebraat spesies wat vir 14 dae in akwaria blootgestel is aan see-water met 200 en 400 p,g/L CdCh, en daama gedekontamineer is in skoon seewater. / The NRF and the University of Stellenbosch, for funding this study.