The Great Plains piping plover (Charadrius melodus) population was listed as threatened under the Endangered Species Act in 1986. The challenge for managers of this population is to increase the fledging rate and to ensure that there is sufficient habitat to support an increasing population. According to the revised recovery plan, there are insufficient quantitative data that relate habitat characteristics to reproductive success in riverine habitat. In particular, additional data are needed to determine if piping plover reproduction is limited by food abundance at breeding sites. The goal of this research was to determine whether piping plover chick survival was limited by foraging site quality on the Missouri River. Productivity has historically varied among river reaches. The alkali wetlands, reported to be very productive, serve as an index to the upper limit of reproductive potential. We compared indices of foraging site quality, chick growth and survival among a reservoir, two river reaches and alkali wetland sites from 2001-2003. We compared piping plover use of protected shoreline and exposed shoreline to availability along two reaches of the Missouri River (Gavins and Garrison). We also examined piping plover chick diet. Chick weight gains were higher at alkali wetlands than Gavins and Garrison. Invertebrate numbers were higher at the alkali wetlands than the three river sites and lowest downstream of the cold water release dam. Invertebrate biomass was highest at the alkali wetlands and downstream of the warm water release dam and lowest downstream of the cold water release dam. Invertebrate biomass downstream of the cold water release dam was the lowest of all 4 sites. There was no relationship between chick daily survival rates and invertebrate biomass or predator presence. Although Diptera occurred more frequently (23% - 64%) at all sites (sticky traps), Coleoptera occurred most frequently in the fecal samples (fecal analysis; 69% to 89%). On the Missouri River, protected shorelines were important foraging sites for piping plovers during the breeding season. We also found that sandbars with low-lying moist habitat are important to foraging chicks. Results are consistent with the prediction that foraging conditions downriver from cold water release dams are inferior to conditions in other habitats in the region. Differences in prey availability are reflected in chick growth rates, but not in survival rates. These results underscore the variability in different indices of habitat quality (invertebrate indices, predation, growth rates, survival rates) among the four sites, and the need for monitoring and management strategies that address each site individually. When managing or creating sandbars, ensuring the availability of protected shoreline will benefit piping plovers on the Missouri River. / Ph. D.

Thesis (PhD (Conservation Ecology and Entomology))--Stellenbosch University, 2008. / The Cape Peninsula is an area of outstanding biological importance, having 158 endemic angiosperm species in only 470 km2. The peninsula invertebrates are known to be highly endemic, yet very little else is known about them. This study has four components: (1) ascertaining whether the influence of environmental variables on epigaeic, foliage and aerial invertebrate assemblages of Table Mountain, (2) determinination of the influence of Table Mountain’s fires and land transformation, on the local invertebrate assemblage, (3), identification of areas of conservation priority and influential environmental variables across the entire Cape Peninsula, and (4) determinination whether invertebrate food availability is a restricting factor for the localised and threatened Knysna warbler (Bradypterus sylvaticus), which inhabits the forests on the east side of Table Mountain. Epigaeic, foliage and aerial invertebrates were intensively sampled using a suite of techniques. Sites were chosen to allow for comparisons between vegetation structure and type, elevation and aspect. Vegetation structure and elevation were the most important environmental variables in determining species composition. Fynbos had a higher beta diversity of epigaeic and aerial invertebrates than forests, so the conservation of as much fynbos as possible is needed. The forests had many unique and endemic species, highlighting their conservation importance. As elevation had a strong influence, yet only the higher elevations receive extensive conservation, it is critical to conserve as much of the disturbed and fragmented lower elevations as soon as possible. There was little correlation between the diversity of aerial and epigaeic‐foliage invertebrate assemblages. Thus, the aerial and epigaeic‐foliage invertebrate assemblages need to be assessed separately.

To determine the relationship between the abundance and density of benthic invertebrates, and the critical shear stress of individual grain sizes, a reach along Smith Creek, was divided into ten 2m x 2m quadrants. Within each quadrant, five randomly selected clasts for each grain size ranging from 2.26 to 25.6 cm were cleaned for benthic invertebrates. Wolman pebble counts for each quadrant were also conducted and used to determine the critical Shields stress per grain size fraction from the model given by Wiberg and Smith (1987) that explicitly accounts for particle hiding/sheltering effects in mixed-bed rivers. Particle entrainment values were then compared with estimated bankfull Shields stress values to determine sediment transport potential during bankfull flow. Invertebrate abundance was strongly positively correlated with critical Shields stress up to the 18.0 cm grain size, indicating a preference for certain grain sizes; while density was positively correlated with all grain sizes present.

Mining is a widespread industrial activity that in many cases, via mining wastes, leads to altered concentrations of metals in close vicinity to the mining activities. Metals in mining waste can have high toxicity and may persist in environments for long time periods. The presence of metals, such as lead (Pb), is known to contaminate and cause damage to nearby organisms and ecosystems. Birds are at risk of metal contamination and, since they are predators high up in the food chain, may face accumulation of metal in tissue over time, via consumption of contaminated prey. Small passerine species, such as the pied flycatcher (Ficedula hypoleuca), are suitable for studying metal contamination as they are ubiqutious, and high up in the food chain. Pied flycatchers feed exclusively on invertebrates, making the abundance, quality, and potential contamination of invertebrates interesting to study with regard to the health of the birds. Studies have shown that hemoglobin (Hb) levels in young pied flycatchers are reduced by high background levels of Pb.  This may be linked to prey availability and quality, as invertebrates are known to alter their composition, and contain higher Pb concentrations in Pb contaminated areas. Here, I investigated how invertebrate abundance and community composition, and pied flycatcher Hb concentrations (i.e. health), in reference and Pb contaminated areas, were related to each other. Invertebrate traps were set and sampled twice during the summer of 2018 to provide invertebrate data to the study. Bird Hb levels was acquired by taking blood samples from nearly fledged chicks in birdhouses placed in the different areas. I found a potential trend towards higher Hb levels in reference areas (p=0.110), suggesting that bird health is reduced by the presence of Pb, but this could not be explained by differences in invertebrate community composition or abundance (p>0.05). Hence, based on this study, high Pb concentrations in the soil does not directly, or indirectly via potential impacts on the prey community composition, influence the health of pied flycatchers.

Astrocytes are the most abundant non-neuronal cells in vertebrate brains. Although Drosophila melanogaster has fewer astrocytic cells relative to neuronal and other glial cell populations, they, like vertebrate astrocytes, are located in synaptic regions, organized into exclusive, minimally-overlapping domains, and play developmental roles in synaptogenesis. But, do Drosophila astrocytes have parallel roles in the regulation of synaptic signaling? Preliminary electron microscopic (EM) data indicates that astrocytic processes are located at a greater distance, on average, from Drosophila synapses than they are from vertebrate synapses, thus raising questions about their capacity to alter synaptic signals. Do astrocytic cells and processes occupy stereotyped synaptic regions across repeating segmental structures and across individuals? In the studies presented here, we have addressed these questions directly in the ventral nerve cord (VNC) of the third-instar larva. We collected the first whole-cell patch-clamp recordings from Drosophila astrocytes. These indicate that intrinsic membrane properties, such as low membrane resistance, high capacitance, a hyperpolarized resting potential relative to neurons, a passive current-voltage relationship, coupling to other astrocytic cells, and an absence of voltage-gated currents, are shared between astrocytes of highly divergent species. Next, we optogenetically activated of a group of glutamatergic pre-motor neurons and showed that astrocytes respond with a glutamate transporter current that is mediated by Eaat1, and that acute, pharmacological and chronic, genetic blockades of this transporter have subsequent effects on the decay of post-synaptic motor neuron currents. Then, we used three-dimensional EM to locate the pre-motor glutamatergic neurons that were activated in the physiological study and measured the distance from each presynaptic site to the nearest astrocytic process. We found that these distances vary 100-fold even along a single neurite and that these structures are rarely in direct contact, but that no synapse is positioned greater than one micron from an astrocytic process. Thus, it is in this anatomical configuration that the regulation of post-synaptic currents by Eaat1 occurs. Finally, we generated a library of single, fluorescently-labeled astrocytes that were co-labeled with fiduciary landmarks, and used this library to compare the placement of astrocyte cell bodies and arbors across VNC segments and individuals. We found substantial variation in the gross shape, size, and territory covered by astrocytes, and conclude that their neuropil domains are not reliably stereotyped. Given the consistent placement of neuronal connectome elements, this indicates that signals of a specific synapse are not regulated by a designated astrocyte. Together, these findings reveal new functional parallels between Drosophila and vertebrate astrocytes. These findings argue for the relevance and applicability of mechanistic discovery in Drosophila astrocytes, and set the stage for further inquiry into the genetic determinants of astrocyte morphology and physiology.

An increase in attachment of SRBCs to Procambarus clarkii hemocytes has been shown after the crayfish were injected with a live or killed Pseudomonas strain RS2b. The increase in attachment occurred at 8 hours post injection and peaked at 24 hours for both experimental groups. The population of hemocytes with receptors for LPS and mannose also increased at 8 hours post injection and peaked at 24 hours for both experimental groups. At 96 hours post injection the number of receptor bearing hemocytes and hemocytes bound to SRBCs began to decrease to the level of the control for both groups. The protein concentration of hemolymph from the experimental groups remained stable at 8 and 24 hours post injection and increased at 96 hours. The correlation of the protein concentration increase at 96 hours with the decrease of receptor bearing hemocytes may be due to the degranulation of the receptor bearing hemocytes.

Thesis (M. Sc. (Zoology)) -- University of the North, 1994 / Refer to the document

Catchment geology is known to influence water chemistry, which can significantly affect both species composition and ecosystem processes in streams. However, current predictions of how stream water chemistry varies with geology are limited in both scope and precision, and we have not adequately tested the specific mechanisms by which water chemistry influences stream biota. My dissertation research goals were to (1) develop empirical models to predict natural base-flow water chemistry from catchment geology and other environmental factors, (2) extend these predictions to nutrients to establish more realistic criteria for evaluating water quality, and (3) test the hypothesis that catchment geology significantly influences the composition of stream invertebrate assemblages by restricting weak osmoregulators from streams with low total dissolved solids (TDS). To meet goal 1, I first mapped geologic chemical and physical influences by associating rock properties with geologic map units. I then used these maps and other environmental factors as predictors of electrical conductivity (EC, a measure of TDS), acid neutralization capacity, and calcium, magnesium, and sulfate concentrations. The models explained 58 – 92% of the variance in these five constituents. Rock chemistry was the best predictor of stream water chemistry, followed by temperature, precipitation and other factors. To meet goal 2, I developed empirical models predicting naturally occurring stream total nitrogen and total phosphorus concentrations. These models explained most of the spatial variation among sites in total nitrogen and phosphorus and produced better predictions than previous models. By determining upper prediction limits that incorporated model error, I demonstrated how predictions of nutrient concentrations could be used to set site-specific nutrient criteria and accounted for natural variation among sites better than regional criteria. To meet goal 3, I experimentally manipulated (high and low) EC in both stream-side and laboratory flowthrough microcosms and measured survival, growth, and emergence of 19 invertebrate taxa. Observed variation among taxa in survival between treatments predicted taxon EC optima estimated from field observations (r² = 0.60). Taxa with the greatest differences in survival between treatments also had the highest EC optima, indicating that the inability to persist in low EC likely restricts the distributions of some taxa.

This thesis examines the comparative abundance of shellfish from archaeological assemblages on the west coast of Vancouver Island in Nuu-chah-nulth territories. Eighteen sites spanning the Nuu-chah-nulth region emphasize the diversity in invertebrate foods that have been consumed 5000-150 years ago: Yaksis Cave, Loon Cave, and Hesquiat Village at Hesquiat Harbour; Chesterman Beach; Spring Cove; Ts’ishaa, Ch’ituukwachisht (North and South), Tl’ihuuw’a, Shiwitis, Huumuuwaa, Maktl7ii, Huts’atswilh, Kakmakimilh, Kiix7iin, and Huu7ii. Invertebrate zooarchaeology is an understudied field that has the potential to impact ecological restoration and conservation efforts. Ubiquity, or frequency of occurrence, provides a measure of abundance for a target taxa or species through a percent presence/absence approach. Regionally conventional methods of invertebrate analysis, including weight-based quantification, primarily favour heavy and robust bivalves, such as clams and mussels, and diminish the presence of other frequently occurring invertebrates. Ubiquity-based quantification shows how frequently ‘other’ shellfish have been utilized over time and across archaeological deposits. Gooseneck barnacles (Pollicipes polymerus) are often considered rare, an unimportant intertidal resource, but ubiquity-based analyses show that they are far more abundant than previously appreciated. A methodological combination of these two approaches shows vastly different perspectives on shellfish abundance, and this has implications for how the dietary role of shellfish is understood and discussed in archaeological discourse. / Graduate / 2020-09-09

Marine ecosystems are increasingly being subject to human impact from diverse recreational and commercial activities, not necessarily restricted to those of a marine nature. This has significant implications for biodiversity. The large dog cockle, Tucetona laticostata, once occurred live in Rangitoto Channel, Hauraki Gulf, although this species no longer appears to occur there, most likely as a consequence of repeated dredging and channel excavation and continued siltation. Tucetona laticostata still occurs in a few isolated pockets of sea bed throughout Hauraki Gulf, particularly off Otata Island, part of the Noises complex of islands, where it resides partially buried in shell and rock gravels in shallow water (to 15 metres depth). The shells of T. laticostata collect in large post-mortem deposits in an area ramping from the sea bed off southwestern Otata Island. These mounds differ significantly in structural complexity from those of adjacent, extensively fragmented shell and rock gravels. Using the mounds of T. laticostata shell as a proxy for structural complexity, in order to appraise the effect of complexity on benthic-invertebrate diversity, the sea bed off southwestern Otata Island was sampled quarterly at two depths and in both T. laticostata shell mounds and adjacent extensively fragmented shell and rock gravels. These data were complemented with those from additional surveys around Otata Island, and off eastern Motutapu Island to determine the distribution and composition of benthic-invertebrate community assemblages throughout the region, and from concurrent surveys throughout the Waitemata Harbour and inner Hauraki Gulf to determine the current distribution of T. laticostata in this region. The number of benthic invertebrate species and individuals within T. laticostata habitat almost always was higher than that occurring within extensively fragmented shell- and rock gravel habitat, with densities to 142,385 individuals m-2 encountered. Temporal and spatial variations in benthic community structure also are reported for the two habitats, T. laticostata-based shells and extensively fragmented shell- and rock gravels. The numbers of species were higher amongst samples collected off the southwestern and eastern sides of Otata Island than elsewhere around this island, or of eastern Motutapu Island. Of the 351 species reported from all Otata and Motutapu Island samples combined, 73% of them occurred off southwestern Otata Island, 30% of which were found exclusively within T. laticostata shell habitat, and 10.5% within extensively fragmented shell and rock gravel habitat. The sea bed off southwestern Otata Island is regularly, seasonally dredged by recreational scallop fishers, in addition to being a popular small-vessel anchorage site. Both of these activities, dredging and anchorage, stand to reduce substratum complexity by fragmentation and dispersal of the valves of T. laticostata. Given the unique benthic invertebrates reported from T. laticostata shell deposits reported from southwestern Otata Island, any activity that damages the shells of this species, regardless of whether they are live or dead, is likely to result in loss of biodiversity. Admittedly, many of species identified as major contributors to differences in benthic invertebrate assemblages between T. laticostata shell-based habitats and those of extensively fragmented shell and rock gravels are not particularly charismatic or large, but each likely plays a role in local food webs and/or sediment and water column chemistry. It was not the intention of this research to determine the effects of anthropogenic disturbances like dredging or vessel anchorage on benthic-invertebrate communities off southwestern Otata Island. However, given the reported differences in species diversity within the structurally complex substratum provided by T. laticostata, conservation of biogenic reef-forming species like it might be a prudent, precautionary measure to take.