Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-09-19T19:18:26Z No. of bitstreams: 1 2016 - Cl?udia dos Reis Ferreira.pdf: 1425765 bytes, checksum: d847a95493845c76aaaf6f7635662069 (MD5) / Made available in DSpace on 2018-09-19T19:18:26Z (GMT). No. of bitstreams: 1 2016 - Cl?udia dos Reis Ferreira.pdf: 1425765 bytes, checksum: d847a95493845c76aaaf6f7635662069 (MD5) Previous issue date: 2016-04-25 / CAPES / To manage properly the natural resources and also to produce with sustainability is a big challenge. Conservation management practices, such as no-tillage system (NTS), intend to increase the sustainability of agricultural systems through improvement of chemical, physical and biological soil attributes. This work was developed in private rural systems, explored commercially in Guaira municipality ? Paran? State. Three different areas were evaluated, with NTS adoption time of 7, 14 and 23 years, with succession of soybean (summer) and corn/wheat crops (winter) (NTS7, NTS14 and NTS23, respectively), and an area of Atlantic Forest as reference. The sampling was taken in the dry season (September/2013), after harvesting corn, and in the rainy season (February/2014), after harvesting soybean; to evaluate effect of duration of soil usage and management under the different crops in NTS on edaphic attributes. The hypothesis tested were: a) long duration management systems can change physical, chemical and biological soil properties; b) the crop succession with grass/legumes species (corn/soybean) promotes modifications in the groups of soil macrofauna and, consequently, in the compartments of soil organic matter (SOM); and c) the season of sampling (winter and summer) influences the composition of soil macrofauna and in the SOM compartments. They were analyzed chemical attributes and total organic carbon (TOC) and nutrient stocks (Ca, Mg, K and P), particle size, aggregates stability, bulk and particle densities, actual soil moisture, calculated the total volume of pores, and evaluated the edaphic macrofauna, at the depths of 0-0.1, 0.1-0.2 and 0.2-0.3 m. The morphological types of aggregates were identified, establishing three groups: physiogenic, intermediates and biogenic. For the different groups it was measured the relative composition, TOC, and physical and chemical fractionation of organic matter. In general, the variation in chemical properties were related to NTS time and the sampling season, which also reflects the soil coverage. Soybean and corn/wheat succession contributed to increase contents and stocks of carbon, Ca and P in the different NTS chronosequences. Although there was no significant difference between the areas, the increasing bulk density values observed with depth in the NTS23 area, with longer system adoption, indicate compacted layers that should be monitored. The highest density and total richness of macrofauna individuals were found in the NTS14 and NTS23 areas under corn residues, but there were not observed higher indexes of equability, due to dominance of Formicidae and Isoptera groups. The areas with the highest equability values were the reference, in the dry season, and NTS23 in the rainy season, with values to 0.87 and 0.61, respectively. The biogenic aggregates were found in larger proportion in the reference area, while the physiogenics and intermediate predominated in the managed areas. The TOC contents were higher in biogenic aggregates, as well as organic carbon associated with minerals (OCam). The humin fraction showed no significant differences in the dry season, while in the rainy season the humin content was higher in physiogenic aggregates. This result indicates that biogenic aggregates of the dry season change into the physiogenic aggregates found in the rainy season. / Gerenciar adequadamente os recursos ambientais e simultaneamente produzir de forma sustent?vel ? um grande desafio. Pr?ticas de manejo conservacionistas, como o sistema plantio direto (SPD), visam aumentar a sustentabilidade dos sistemas agr?colas atrav?s da melhoria dos atributos qu?micos, f?sicos e biol?gicos do solo. O trabalho foi desenvolvido em sistemas agr?colas particulares, explorados comercialmente no munic?pio de Gua?ra ? PR. Foram avaliadas tr?s ?reas com diferentes tempos de ado??o do SPD, por 7, 14 e 23 anos sob sucess?o das culturas soja (ver?o) e milho/trigo (inverno) (SPD7, SPD14 e SPD23 respectivamente), e uma ?rea de Floresta Atl?ntica como refer?ncia. As amostragens foram feitas no inverno ap?s a colheita das culturas de milho (setembro/2013), e no ver?o, ap?s a colheita de soja (fevereiro/2014). O objetivo foi avaliar o efeito do tempo de uso e manejo do solo sob diferentes coberturas em SPD sobre os atributos ed?ficos. As hip?teses testadas foram: a) sistemas de manejo de longa dura??o podem alterar caracter?sticas f?sicas, qu?micas e biol?gicas dos solos; b) a sucess?o de culturas com esp?cies de gram?neas/leguminosas (milho/soja) promove modifica??es nos grupos da macrofauna do solo e, desta forma, nos compartimentos da MOS; e c) a ?poca de avalia??o (inverno e ver?o) influencia na composi??o da macrofauna do solo e nos compartimentos da MOS. Foram analisados: atributos qu?micos e estoques de carbono org?nico total (COT) e de nutrientes (Ca, Mg, K e P), an?lise granulom?trica, estabilidade de agregados, densidade do solo (Ds) e da part?cula (Dp), umidade atual do solo, calculado o volume total de poros, e avaliada a macrofauna ed?fica, nas profundidades de 0-0,1, 0,1-0,2 e 0,2-0,3 m. Foram identificados os tipos morfol?gicos de agregados, nos tr?s grupos: fisiog?nicos, intermedi?rios e biog?nicos. Para os diferentes grupos, foi medida a composi??o relativa, COT, e fracionamento qu?mico e f?sico ou granulom?trico da mat?ria org?nica. De modo geral, a varia??o nos atributos qu?micos se relacionou com o tempo de implanta??o do SPD e a ?poca de amostragem, que tamb?m refletiu a cobertura do solo. A sucess?o soja, milho/trigo contribuiu no aumento dos teores e estoques de carbono, Ca e P nos diferentes tempos de implanta??o do SPD. Embora sem diferen?a significativa entre as ?reas, os valores de Ds em profundidade na ?rea SPD23, com maior tempo de ado??o, indicam camadas compactadas, que devem ser monitoradas. A maior densidade e riqueza total de indiv?duos da macrofauna foram encontradas nas ?reas SPD14 e SPD23 sob a palhada do milho; por?m n?o foram observados maiores ?ndices de equabilidade, pelo dom?nio dos grupos Formicidae e Isoptera. As ?reas com maiores valores de equabilidade foram a de refer?ncia, no inverno, e a de SPD23 no ver?o, com 0,87 e 0,61, respectivamente. A maior propor??o de agregados biog?nicos foi na ?rea de refer?ncia; j? os fisiog?nicos e intermedi?rios predominaram nas ?reas manejadas. Os teores de COT foram maiores nos agregados biog?nicos, assim como o carbono org?nico associado aos minerais (COam). A fra??o humina n?o apresentou diferen?a significativa na coleta de inverno; no ver?o, a humina foi maior nos agregados fisiog?nicos. Esse resultado indica que os agregados biog?nicos do inverno originaram os agregados fisiog?nicos encontrados no ver?o.

No description available.

Inland salt lakes in Western Australia have been used by the mining industry for the disposal of excess water generated during the mining process. However, the impact of these operations on the salt lakes is poorly understood. This is mainly due to the lack of information on the biota and chemistry for the lakes. The main aim of this project was to develop a classification system for inland salt lakes of Western Australia based on abiotic and biotic factors such as sediment and water quality, invertebrates and algae to determine lakes with unique or significant features. Water and sediments collected from the salt lakes were generally characterised by an alkaline pH, high salinity and the majority of lakes being dominated by sodium and chloride. Concentrations of some metals were also high, particularly in surface water. A high degree of variation in water and sediment quality was demonstrated both within and between the study lakes. In addition, these parameters were shown to be influenced by geography, geology, stage of the hydrocycle within which the lake was sampled and the occurrence of dewatering discharge. Biota in the salt lakes must be able to cope in a harsh environment, adjusting to temporary water regime, high temperature, and high salinity. As such, the species richness of these systems is generally low. Diatoms (a group of algae) and invertebrates were investigated among the biota. A total of 56 diatom species were recorded from 24 lakes. The most common species were Amphora coffeaeformis, Hantzschia aff. baltica and Navicula aff. incertata. These species were shown to have broad tolerances to environmental variations. Sediment chemistry explained variations in diatom community structure, with zinc, moisture content and cobalt having the greatest and negative influence. / In terms of invertebrates, a total of 101 invertebrate taxa were recorded from 13 lakes in this study. Crustacea dominated and the greatest number of taxa was from the genus Parartemia. There were some differences in invertebrate community structure between lakes, most likely reflecting the high degree of speciation, and poor dispersal mechanisms of certain key species. Community structure was influenced by water quality, with phosphorus, bicarbonate and magnesium contributing to the variations in community structure. Among the 43 lakes chosen for this study a total of 17 lakes had received, or are currently receiving dewatering discharge. Sites receiving dewatering discharge generally reported higher concentrations of salts, nutrients and some metals in both water and sediments compared to natural lakes. Species richness of biota such as diatoms and invertebrates was lower at the lakes receiving dewatering discharge. However, the impact was generally localized within the pooled area of dewatering discharge. Also, despite these impacts, there appears to be signs of amelioration by flushing events. Currently there are no guidelines for water and sediment chemistry for inland salt lakes in Western Australia. Australian and New Zealand Environment and Conservation Council (ANZECC) guidelines are the most relevant available. Concentrations of cadmium, cobalt, chromium, copper, lead, nickel and zinc in surface water of the natural inland salt lakes were shown to exceed ANZECC guideline values. / Comparison with the relevant ANZECC sediment guidelines showed that they were applicable to the salt lakes, with the exception of nickel and chromium which were naturally high in the salt lake sediments. Classification of data using multivariate analysis was done for both dry and wet phases of the hydroperiod. Six groups were delineated for the sediment and diatom data, and four groups were defined for the water quality and invertebrate data. It was common for sites from particular lakes to fall in more than one group as a result of the variability in these systems. There are a number of practical applications of this system for the mining industry and it may be used as a predictive tool for determining the impact of dewatering discharge and highlighting unique salt lakes within the Goldfields of Western Australia.

This study is an interdisciplinary ecological study addressing the fine-scale relationships between plants, invertebrates and the environment in an alpine ecosystem. Alpine environments are marked by steep environmental gradients and complex habitat mosaics at various spatial scales. Regular forming periglacial patterned ground landforms on the Old Man Range, Central Otago, South Island, New Zealand present an ideal medium for studying plant-invertebrate-environment relationships due to their partitioning of the landscape into discrete units of contrasting environmental conditions, and the existence of some baseline knowledge of the soil, microclimate, vegetation and flora. The study was conducted in three types of patterned ground (hummocks, stripes and solifluction terraces) on the Old Man Range. Each component of the study was sampled at the same spatial scale for comparison. Temperature was recorded in the soil and ground surface from April 2001 to March 2004 in microtopographic subunits (microsites) of each patterned ground landform. Plant species cover was sampled within each microsite; invertebrates were sampled from soil cores taken from the same locations as plant samples in April 2001 and September 2001. The two sampling occasions coincided with autumn before the soil freezes, and winter when maximum freezing was expected. Fine-scale changes in the topographic relief of the patterned ground led to notable differences in the timing and duration of snow. The steepest environmental gradients existed during periods of uneven snow distribution. The soil in exposed or south-facing microsites froze first, beginning in May, and typically froze to more than 40cm depth. Least exposed microsites rarely froze. Within the microtopography, patterns of freezing at specific locations were consistent between years with only minor differences in the timing or depths of freezing; however, notable variation in freezing existed between similar microsites. Within the microtopography, different assemblages of organisms were associated with different microsites. In total, 84 plant and lichen species were recorded, grouping into six community types. Species composition was best explained by growing degree-days, freeze-thaw cycles, time frozen and snow-free days; species diversity and richness increased with increasing environmental stress as indicated by freeze-thaw cycles, time frozen and exposure. In total 20,494 invertebrates, representing four Phyla, 12 Classes, 23 Orders and 295 morpho-taxa were collected from 0.17m� of soil. Acari, Collembola and Pseudococcidae were the most abundant invertebrates. Over 95% of the invertebrates were found in the plant material and first 10cm depth of soil. Few significant relationships were found between diversity, richness or abundance of invertebrate taxa and the microsites; however, multivariate analyses identified distinct invertebrate assemblages based on abundance. Invertebrate composition was best explained by recent low temperature and moisture, particularly in winter; however, plant composition also explained invertebrate composition, but more so in autumn. This research has shown that organisms in the alpine environment of the Old Man Range are sensitive to fine-scale changes in their environment. These results have implications as to how historical changes to the ecosystem may have had long-lasting influences on the biota, as well as how a currently changing climate may have further impacts on the composition and distribution of organisms.

Three approaches were employed to examine the effects of elevated sediment trace metal concentrations on estuarine/marine macrobenthic invertebrate assemblages. The initial study examined macroinvertebrate communities along a known polymetallic gradient, Lake Macquarie, NSW (gradient study). The second study experimentally tested if sediments sourced from different locations within Lake Macquarie differentially influenced the recolonisation of benthic invertebrates. The third study investigated the different recolonisation patterns of benthic invertebrates into sediments spiked with increasing concentrations of sediment-bound cadmium. In the Lake Macquarie gradient study, four locations (Cockle Bay, Warner's Bay, Kooroora Bay and Nord's Wharf) were sampled in winter 2000 and summer 2003 using a hierarchical design (location > site > plot). On both sampling occasions, the sediments showed strong gradients in lead, cadmium and zinc concentrations emanating from the Cockle Bay industrialised region in the lake's north, with concentrations being significantly lower in the most southern and less urbanised location (Nord's Wharf). In general, concentrations of lead, cadmium and zinc in the sediments increased among locations in the following order: Nord's Wharf > Kooroora Bay > Warner's Bay > Cockle Bay. AVSJSEM analyses indicated that in some sites in Cockle Bay, and to a lesser extent Warner's Bay, SEM concentrations exceeded their molar equivalence of AVS, indicating the potential for trace metals to be labile within the porewaters. Granulometry also changed along the gradient, with a higher proportion of silt/clay occurring in the locations with high metal concentrations. Conversely, the percentage of total organic carbon was higher in the less contaminated locations. In winter 2000, changes in benthic communities along the gradient supported the a priori hypotheses, with diversity and richness being greater in locations with lower concentrations of metals. Polychaetes were most numerous in Cockle Bay and Warner's Bay, whilst bivalves and gastropods were more abundant in Nord's Wharf and Kooroora Bay. Crustaceans were more numerous in Nord's Wharf; with all other locations having similar, lower, abundances. Ordination maps of the assemblages provided relatively clear separation of the assemblages among locations, with nonparametric multivariate analysis of variance (NPMANOVA) and subsequent pair-wise comparisons finding significant differences among the assemblages from all locations. SIMPER analyses found the highest level of dissimilarity was between the Nord's Wharf and Cockle Bay assemblages - primarily attributable to differences in the relative contributions of isopods; tellenid bivalves; and the polychaete families Spionidae, Opheliidae and Nephytidae. Weighted Spearman rank correlations (BIOENV) identified cadmium (Pw =0.74) as the strongest environmental (single or combination) variable to correlate with biotic assemblages. Benthic patterns along the gradient were less defined in summer 2003 due to a dramatic reduction in the abundance and diversity of fauna in Nord's Wharf. This decline was possibly attributable to a sustained reduction in salinity caused by a prolonged rainfall event. With the exception of Nord's Wharf, trends in the community indices and abundances of key taxa among the other locations were similar to those reported in winter 2000. Multivariate analyses discriminated the benthic assemblages from the four locations, with the findings from the NPMANOVA pair-wise comparisons indicating that the assemblages from all four locations were significantly different. SIMPER analyses showed the highest level of dissimilarity was between Nord's Wharf and Warner's Bay, with these differences being primarily attributable to their relative abundances of amphipods and polychaetes from the families Spionidae, Cirratulidae, Opheliidae and Capitellidae. BIOENV found that the combination of the sedimentary concentrations of cadmium and iron provided the best correlation (Pw =0.73) with biotic patterns, with similar correlations occumng with the addition of lead and its covariate, zinc (Pw =0.72). The combined findings from the gradient study established a strong correlation between trace metal concentrations within the sediments and suite of univariate and multivariate measurements. The low abundance and diversity of fauna in Nord's Wharf in the summer of 2003 highlighted the dynamic changes which can occur in the distributions of macrobenthic invertebrates. Although the study indicated that there was a strong relationship between trace metal concentrations and benthic community structure, the study was correlative, and requires subsequent experimental testing to confirm the causality of the observed relationships. The second component of the research was a translocation experiment using benthic recolonisation as an end-point. The experiment was performed to identify if the sediments, and not location, were influencing the composition of benthic assemblages in Lake Macquarie. Sediments were collected from three locations (Cockle Bay, Warner's Bay and Nord's Wharf), defaunated, and transplanted in three new locations along the south-east edge of the lake. At each location, 10 containers of each treatment were randomly placed in the sediment and allowed to recolonise for 22 weeks. Upon retrieval, the benthic communities were sampled and enumerated in conjunction with a variety of chemical and sedimentary measurements. Ten replicate invertebrate samples were also collected in the sediments adjacent to the experiment (ambient samples) at the completion of the experiment. Due to human interference, the containers from only two locations were analysed. Upon retrieval, pH and redox profiles of the sediments were similar to those expected in natural sediments. In general, concentrations of metals were low in the porewaters; however, iron precipitation on the porewater collection devices may have artificially increased the diffusion of metals, increasing concentrations near the sediment-water interface. Concentrations of SEM exceeded their AVS equivalence in some samples taken from the Cockle Bay and Warner's Bay treatments. Two-way ANOVAs found significant interactions between location and sediment treatments in diversity, evenness and the number of polychaetes, as well as significant differences in the number of capitellids and crustaceans among locations. Post-hoc comparisons of means found the Nord's Wharf sediment contained a higher mean number of individuals than the other treatments, including the ambient samples. nMDS ordination plots for both locations provided poor graphical discrimination of the assemblages among treatments; however, NPMANOVA detected significant location and treatment interactions. In both locations, pair-wise comparisons indicated that the assemblages within the Nord's Wharf treatments were significantly different to the Cockle Bay, Warner's Bay and ambient assemblages. No significant differences were detected between the Cockle Bay and Warner's Bay assemblages at either location. SIMPER analyses found the highest level of dissimilarity occurred between the ambient assemblages in Location 2 and the Nord's Wharf treatment, primarily due to the relative difference in the abundances of Capitellidae, Spionidae, Oweniidae, Nereididae and isopods among the assemblages. The findings from the translocation experiment suggest that the sediments are influencing the recolonisation of benthos. However, because differences were not detected between the Cockle Bay and Warner's Bay treatments, the approach used in the study shows potential as an in situ technique which could be used to assess the potential ecological risks of sediments fiom specific locations. Excluding cost and time considerations, the technique's primary disadvantage is the lack of a true control. As a result, the technique can only identify if the sediments are modifying benthic recolonisation, and not causality. The final component of the research experimentally tested if elevated concentrations of sediment-bound cadmium affected benthic invertebrate recolonisation. Sediments from the south coast of New South Wales (Durras Lake) were defaunated, and spiked with cadmium under anaerobic conditions to obtain three targeted cadmium concentrations: control (<O.1 ug/g), Low-Cd (15 Cd ug/g) and High-Cd (150 Cd ug/g). The physio-chemical properties of the waters and porewater concentrations of cadmium were monitored over a 28-day equilibration period, with declines in pH mediated with the addition of NaOH(aq). At the end of the equilibration period, porewater concentrations of cadmium were low in the Low-Cd and High-Cd treatments (maximum <l.5 ug/L in High-Cd), and below the detection limit in the control. Cadmium was not detected in the control sediments, with concentrations in the Cd-Low and Cd-High sediments exceeding their targeted concentrations, with final mean concentrations of 17 ug/g and 183 ug/g, respectively. The experimental design was similar to that employed in the translocation experiment, with 10 containers from each treatment transplanted into the sediments at three locations within Lake Macquarie. After 20 weeks, the containers were collected, along with benthic invertebrate samples from the ambient sediments. Data was not used from Location C due to extensive sediment deposition on the transplanted treatments. Significant declines occurred in the concentrations of cadmium in both the Low-Cd and High-Cd sediments, with the greatest loss occumng in the surficial sediments. The loss of cadmium was probably due to the differential loss of the fine fraction through physical means (hydrodynamic) rather than fluxing, as it assumed that the cadmium was primarily sediment-bound and relatively insoluble under anoxic conditions. Mean porewater concentrations of cadmium were below the detection limit in the control treatments; < 1 ug/L in the Low-Cd treatment, and generally < 2ug/L in the High-Cd, with the exception of some samples in Location B (maximum 5.6 ug/L) Concentrations of ammonia were low in the porewaters from the surficial sediments, with concentrations being significantly higher, and potentially toxic, in the anoxic porewaters (7 cm depth). In comparison to the previous recolonisation experiment, the number of individuals which recolonised the cadmium-spiked treatments was low, and significantly lower than the mean number of individuals sampled in the ambient sediments. No significant differences were detected among the treatments or locations (and their interactions) in diversity (H'), richness (d) or evenness (J). The number of polychaetes and molluscs significantly differed among the treatments, with post-hoc analyses indicating these differences were not among the cadmium-spike treatments, but were due to a greater mean abundance of these taxa in the ambient sediments. A significant interaction between treatment and location was detected in the mean abundance of crustaceans, with the ambient sediments having significantly lower mean abundances in both Location A and B. Ordination plots of the experiments in Location A and B provided poor graphical discrimination among the spiked treatments, although the ambient assemblages appear to be separated from the cadmium-spiked assemblages. NPMANOVA detected a significant interaction between treatments and locations, as well as among treatments. In both Location A and B, pair-wise analyses found the assemblages in the ambient sediments to be significantly different to the assemblages in all three cadmium treatments, with no differences being detected among the latter. SIMPER analyses found the highest levels of dissimilarity occurred between the spike-treatments and the ambient sediments, with these differences being primarily due to the relatively higher abundance of decapods in the spiked treatments, and capitellids in the ambient sediments. The cadmium-spiking component of the experiment clearly illustrated that artificially increasing the trace metal concentrations of metals in estuarine sediments is a complex process which needs to be performed in a methodological manner in order to obtain homogenous treatments with low porewater concentrations, and minimal artefacts. Furthermore, the results confirmed that the equilibration time for sediments can be extensive (several weeks), even in the case of organically rich sediments. The timing of the experiment (commenced late summer, February, 2003) appears to the major factor for the relatively low recolonisation rates, with the experiment missing the main larval recolonisation period between spring and early summer. Even in the highest treatment, elevated concentrations of cadmium did not appear to affect benthic recolonisation. This finding is supported by other experimental studies which suggest that concentrations of a single isolated metal must considerably exceed current guideline values (or contain high porewater concentrations) in order to elicit a biological effect. Nevertheless, as trace metals generally co-occur with other contaminants - with the response of multiple contaminants being possibly additive or synergistic - a conservative guideline value may be suitable in the interim as a precautionary measure. The findings of this thesis suggest that elevated concentrations of trace metal mixtures in estuarine sediments can affect the structure and composition of benthic communities; however, identifying causality is difficult. Although there has been an increase in the use of manipulative field experiments as a means of reducing the confounding influence of covariables found in field studies, this approach also has limitations, e.g. spatial and temporal scale issues, container effects, cost and biogeochemical changes to the sediments. Measuring stress at a community level is a fundamental component of estuarine risk assessment programs; and in isolation this approach can produce subjective and confounded findings. In order to accurately assess the risks associated with trace metal contaminated sediments, an integrated approach (e.g. weight of evidence) is required, one which uses multiple lines of evidence sourced from various chemical, environmental biological measurements.

<p>Effekterna på öringens (Salmo trutta) diet till följd av en avverkning av kantzonsvegetationen har undersökts i ett fältexperiment, där två av de potentiella effekterna av en skogsavverkning simulerades. Fokus låg på de två faktorerna ökad ljusinstrålning och minskat nedfall av terrestra evertebrater. Ökad ljusinstrålning har simulerats med hjälp av montage av lysrör och nedfall av terrestra evertebrater med hjälp av plasttält. Frågeställningarna rör andelen terrester föda i olika behandlingar och årstider, dietskillnader mellan äldre och yngre öringindivider, skillnader i mängden akvatisk föda vid ökad ljusinstrålning samt en taxonomisk översikt av dietens sammansättning. Signifikanta skillnader mellan behandlingarna fanns, där individerna i kontrollbehandlingen åt signifikant mer terrester föda än individer i tältbehandlingen och den kombinerade tält- och ljusbehandlingen. Signifikanta skillnader fanns även mellan provtagningstillfällena där en högre andel terrester föda återfinns senare på säsongen. Inga signifikanta skillnader fanns med avseende på andelen terrester föda mellan äldre och yngre individer, ej heller mellan mängd akvatisk föda mellan de olika behandlingarna. Den akvatiska födan var mer varierad och divers än den terrestra, och daggmaskar (Lumbriculidae) var en mycket vanlig födopartikel.</p> / <p>The effects on the diet of brown trout (Salmo trutta) of a clear cutting of the riparian vegetation has been investigated in a field-experiment, where two of the potential effects of a clear cut were simulated. The focus was placed on the two factors increased light and input of terrestrial invertebrates. The increase of ligth was manipulated by placing fluorescent lights over the brook and the input of terrestrial invertebrates was manipulated by placing plastic tents over the brook. Issues discussed are the percentage terrestrial food in the different treatments and at the different times of sampling, differences in the percentage terrestrial food between older and younger individuals of brown trout, differences in the amount of aquatic food in the light-treatment and a taxonomic summary of the composition of the diet. There were significant differences between the the different treatments, where the individuals in the control treatment ate significantly more terrestrial food as compared to the individuals in the tent and in the combined light and tent treatment. There was also a significant difference between the different times of sampling, more terrestrial food was consumed later in the season. No sigificant differences were detected between older and younger individuals concerning the percentage of terrestrial food eaten, nor between the different treatments concearning the amount of aquatic food consumed. The aquatic food showed a wider variation and was more diverse as compared to the terrestrial food, and earthworms (Lumbriculidae) was a very common food item.</p>

The current environmental status of the majority of Bermuda’s one hundred sixty-six known caves was investigated. This survey replicated a historical cave study performed in 1983, wherein each was analyzed for positive and negative features. Statistical analysis of the data showed a significant difference between the historical and current survey ratings, with an overall decrease in environmental status. A water quality study was performed on twenty different caves with sea level pools in various locations around the island. Nitrate, nitrite, ammonia, and phosphate levels were measured from varying depths in these caves. Fifteen of these caves were also tested for the presence of fecal bacterial contamination. High nitrate levels were discovered in some of the caves, particularly in surface samples. Additionally, bacterial contamination was detected in some caves. No obvious relationship between cave size or location and contamination existed for any of the pollutants sampled. Three separate caves from this group were dived and analyzed using a Hydrolab Sonde 3 Multiprobe Logger to acquire in situ water column data including depth, temperature, pH, salinity, and dissolved oxygen. Each cave studied had its own unique trends in hydrology at varying depths in the water column. A later water sampling study with a randomized experimental design was created and caves were divided into four classes based on size and location. Surface and subsurface samples were gathered from twelve randomly selected caves, three from each class. Each sample was analyzed for nitrate, nitrite, and ammonia concentrations. The results were analyzed using multiple analysis of variance statistics. A significant difference between the nitrate concentrations in the surface and subsurface water samples was discovered. None of the other comparisons were statistically significant. To represent the data visually, a Bermuda Cave and Karst Information System (BeCKIS) was created using the environmental survey data and water quality information. Some of the maps generated highlighted regions where negative environmental impacts on caves were concentrated geographically, thus demonstrating how this geographic information system could be used as a conservation tool.

Effekterna på öringens (Salmo trutta) diet till följd av en avverkning av kantzonsvegetationen har undersökts i ett fältexperiment, där två av de potentiella effekterna av en skogsavverkning simulerades. Fokus låg på de två faktorerna ökad ljusinstrålning och minskat nedfall av terrestra evertebrater. Ökad ljusinstrålning har simulerats med hjälp av montage av lysrör och nedfall av terrestra evertebrater med hjälp av plasttält. Frågeställningarna rör andelen terrester föda i olika behandlingar och årstider, dietskillnader mellan äldre och yngre öringindivider, skillnader i mängden akvatisk föda vid ökad ljusinstrålning samt en taxonomisk översikt av dietens sammansättning. Signifikanta skillnader mellan behandlingarna fanns, där individerna i kontrollbehandlingen åt signifikant mer terrester föda än individer i tältbehandlingen och den kombinerade tält- och ljusbehandlingen. Signifikanta skillnader fanns även mellan provtagningstillfällena där en högre andel terrester föda återfinns senare på säsongen. Inga signifikanta skillnader fanns med avseende på andelen terrester föda mellan äldre och yngre individer, ej heller mellan mängd akvatisk föda mellan de olika behandlingarna. Den akvatiska födan var mer varierad och divers än den terrestra, och daggmaskar (Lumbriculidae) var en mycket vanlig födopartikel. / The effects on the diet of brown trout (Salmo trutta) of a clear cutting of the riparian vegetation has been investigated in a field-experiment, where two of the potential effects of a clear cut were simulated. The focus was placed on the two factors increased light and input of terrestrial invertebrates. The increase of ligth was manipulated by placing fluorescent lights over the brook and the input of terrestrial invertebrates was manipulated by placing plastic tents over the brook. Issues discussed are the percentage terrestrial food in the different treatments and at the different times of sampling, differences in the percentage terrestrial food between older and younger individuals of brown trout, differences in the amount of aquatic food in the light-treatment and a taxonomic summary of the composition of the diet. There were significant differences between the the different treatments, where the individuals in the control treatment ate significantly more terrestrial food as compared to the individuals in the tent and in the combined light and tent treatment. There was also a significant difference between the different times of sampling, more terrestrial food was consumed later in the season. No sigificant differences were detected between older and younger individuals concerning the percentage of terrestrial food eaten, nor between the different treatments concearning the amount of aquatic food consumed. The aquatic food showed a wider variation and was more diverse as compared to the terrestrial food, and earthworms (Lumbriculidae) was a very common food item.

Manmade radionuclides have been introduced to the environment for almost a century. The main source has been the nuclear weapons testing programmes, but accidental releases from the nuclear power production industries have also contributed. The risk to humans from potential releases from nuclear facilities is evaluated in safety assessments. Essential components of these assessments are exposure models, which estimate the transport of radionuclides in the environment, the uptake in biota, and transfer to humans. Recently, there has been a growing concern for radiological protection of the whole environment, not only humans, and a first attempt has been to employ model approaches based on stylised environments and transfer functions to biota based exclusively on bioconcentration factors. They are generally of a non-mechanistic nature and involve no knowledge of the actual processes involved, which is a severe limitation when assessing real ecosystems. The research presented in this thesis attempts to introduce a methodology for modelling exposure of biota that is based on systems ecological theories and concepts. All presented papers concern bioaccumulation and circulation of radionuclides in coastal areas of the Baltic Sea, which is a sea surrounded by several nuclear power plants, waste repositories and reprocessing facilities. Paper I illustrates how an ecosystem model can be used to predict the fate of C-14 in a bay, and to explore the influence of uptake route and water exchange on the concentrations in biota. Due to the longevity of many radionuclides, time spans of thousands of years need to be considered in assessments of nuclear waste facilities. In Paper II, the methodological problems associated with these long timescales are discussed and a new modelling approach is proposed. An extension and generalisation of the C-14 flow model into a generic model for other radionuclides is described and tested in Paper III. This paper also explores the importance of three radionuclide specific mechanisms (plant uptake, excretion and adsorption to organic surfaces) for the concentrations in biota. In Paper IV, the bioaccumulation kinetics of three radionuclides in three key benthic species of the Baltic Sea is studied experimentally. Paper V considers remobilisation and redistribution of sediment-associated radionuclides due to biological mixing, in a microcosm study. The findings in this thesis show both that it was possible to use an ecosystem approach to assess the exposure to biota, and that this approach can handle many of the problems identified in the use of traditional exposure models for radionuclides. To conclude, frameworks for the protection of the environment from ionising radiation would benefit from implementing methodologies based on ecologically sound principles and modelling techniques.

Graduation date: 1990