Pantoea stewartii subsp. stewartii is a bacterial phytopathogen that causes the disease Stewart's wilt in corn. The insect vector Chaetocnema pulicaria, the corn flea beetle, transmits P. stewartii into corn plants through wounds in the leaves. The bacteria can then move to the xylem of the plant where they form a biofilm that inhibits the flow of water. A previous in planta RNA-Seq study resulted in the selection of lrp as a gene of interest for further analyses. A reverse genetics approach was used for the creation of a strain containing the in-frame deletion of lrp, as well as a revertant strain. The strain with the deletion of the lrp gene showed reduced motility and capsule formation when in vitro assays were conducted. It has previously been demonstrated that these characteristics are both important for the bacteria's ability to form a biofilm in the xylem of corn plants and produce disease symptoms. The in planta virulence and competition assays demonstrated that the lrp gene deletion also results in reduced disease symptoms in infected corn plants, as well as an inability to outcompete wildtype P. stewartii in xylem colonization. In a bioinformatics approach, the transcriptional regulator Lrp of P. stewartii was present in the same node of the phylogeny as homologues from other closely related phytopathogens. This demonstrates that Lrp from P. stewartii and such homologues have evolved from a recent common ancestral gene. Examining the genomic islands present in P. stewartii, it is possible to begin to predict where some of the genes which have functions involved in plant colonization may have originated. Overall, the results collected from the studies in this thesis contribute to improving understanding of how P. stewartii is successful at colonizing the xylem of corn plants and cause disease. This research could result in the development of methods to decrease crop susceptibility to infection with P. stewartii. / Master of Science / Stewart's wilt is a disease of corn plants caused by the bacterium Pantoea stewartii subsp. stewartii via the insect vector Chaetocnema pulicaria, the corn flea beetle. This infection has proven to be costly as it impacts the health of corn crops and impedes the export of corn seeds from varieties that are susceptible to infection by P. stewartii. The focus of the research conducted for this thesis has been on learning more about how specific P. stewartii genes impact the ability of the bacterium to colonize corn plants and cause Stewart's wilt disease symptoms. The information collected from this study is important for developing a better understanding of how wilt disease-causing pathogens are able to successfully infect plants, as well as for developing future treatments to prevent further infection of corn plants. In addition, preliminary bioinformatics work has shown that some of the P. stewartii genes of interest share a common ancestor with select genes from other known plant pathogens. Additional preliminary bioinformatics work on regions of the DNA called genomic islands has revealed where some genes of importance to the bacterium's ability to colonize plants may have originated. Overall, the work presented in this thesis contributes to improving our understanding of the roles that different parts of the P. stewartii genome have in allowing the bacterium to successfully colonize and cause disease in corn plants.

A nomadic con artist, bent on inflating the price of his family land, deceives a small Irish town on the verge of bankruptcy into hosting an international sporting event.

Yes / Objectives: This study presents isotopic information for incremental dentine collagen and bone bulk collagen from individuals from the Canary Islands (Tenerife and Gran Canaria) to explore dietary differences during childhood life. Materials and Methods: Eight individuals have been studied, which comprises 122 δ15N and δ13C incremental dentine measurements and eight bulk bone collagen analyses. A baseline of potentially consumed food sources has been developed for comparative purposes. A FRUITS model of probable contributions of each food source towards the diet of each individual has been developed. All samples but one belongs to the later period of indigenous occupation of the archipelago. Results: The dentine collagen data are presented in correlated δ13C and δ15N plots per individual, showing the isotopic changes throughout time. δ15N values for each individual tend to be variable whereas δ13C data are generally more stable with a range of +9.1 to +14‰ for δ15N and –17.4 to –20.8‰ for δ13C. Conclusion: The isotopic analysis allows for the reconstruction of 8 dietary profiles, which allow us to estimate the different dietary protein sources. The FRUITS model shows different percentages of the primary food sources for each individual. Where both δ13C and δ15N are elevated, this could be indicative of a higher marine contribution to the diet. There appear to be two main dietary profiles identifiable in the dataset and these may be related to changes in status or place of residence. Short-term variations in δ13C and δ15N and opposing co-variance of isotopic values can be indicative of nutritional stress, although metabolic changes during growth are also considered. / PhD grant from Universidad de La Laguna—Fundación La Caixa (“Contratos Predoctorales para la formación de doctores ULL-2015”), MINECO (Ministerio de Economía y Competitividad) project “Guanches y europeos en Las Cañadas del Teide, Ocupación, Producción y Comunicación” (HAR2015-68323-P), “Erasmus+ Scholarship” given by the University of La Laguna, ERC Starting Grant project IsoCAN (grant 851733, European Comission) and the projects RTI2018-101923-J-I00 and RYC2019-028346 (Ministerio de Ciencia e Innovación)

Call number: LD2668 .T4 1979 E38 / Master of Landscape Architecture

This thesis examines the politics of local, governance in the Scottish Highlands, taking the Highlands and Islands Enterprise (HIE) network - made up of a central core and 10 Local Enterprise Companies (LECs) - as its institutional focus. It synthesises regulationist approaches and neo-Marxist state theory to explain LECs as part of a broader process of re-regulation under consecutive Conservative governments. LECs are unelected, business-led agencies operating at the local level. The political discourse through which LECs were established and promoted created expectations of local autonomy among business representatives that clashed with the centralising tendencies of Thatcherism. The thesis examines how the resultant tension between local initiative and central control has been worked out within the HIE network. It relies on data collected from seventy semi-structured interviews with representatives of HIE, LECs, local authorities, businesses and community groups. The initial chapters introduce the research and consider key methodological issues, set out the theoretical framework, and review the practices of the Highlands and Islands Development Board (HIDB, HIE's successor). The thesis then explores the key tension between local initiative and central control, explaining how it has been mediated and resolved through routine institutional practices. It also examines HIE-LECs relations with other key agencies, notably local authorities, through selected examples of multi-agency partnerships and assesses LECs' local accountability and representativeness. Finally, a concluding chapter sets out the main findings and considers their implications. While key managerial 'technologies' such as targeting, audit and financial controls allow central government to monitor and steer the HIE network, the thesis argues that the authoritative resources of the HIE core - grounded in the combination of local knowledge and technical expertise inherited from the HIDB - enables it to adapt key aspects of the operating regime to its own purposes. Local autonomy is limited by the relative centralisation of the Network, and LECs operate in a system of structured flexibility in which their scope to adapt policy to local conditions is constrained by state rules and procedures. In emphasising that local autonomy is limited by hierarchical mechanisms of control, the thesis argues that local governance in the Scottish Highlands continues to be underpinned by government. It also points to the limits of the regulation approach and neo-Marxist state theory as theoretical perspectives, suggesting that neo-Foucauldian writings on govemmentality are useful in providing stronger analytical purchase on the specific mechanisms and procedures through which state regulation is practised.

This thesis re-examines the territorial dispute between Japan and Russia, the so-called “Northern Territories” issue, through a reinterpretation of the role of the indigenous Ainu of Japan. An exploration of Ainu history and historiography reveals that the long-standing emphasis on Wajin-based legitimacy of rule and annexation of northern areas was replaced by historical amnesia concerning the role and status of the Ainu. Discussion focuses on an interpretation of Ainu understandings of local, regional/national and international historical events. This approach underscores the importance of de-nationalising History by integrating the important perspectives of Indigeneity. It asserts, further, that the understanding of these events and processes require a broader disciplinary prism than that provided by the study of history. The preponderance of nation-based studies, and not only in the field of History, has seriously inhibited the analysis of historical phenomena involving Indigenous peoples, in this case the Ainu. The study of the Northern Territories issue offers, then, both a new perspective on the history of this important dispute and an illustration of the importance of broadening traditional academic studies in disciplines such as History, Anthropology, Ecology, Political Science, International Relations and Law to incorporate Indigenous perspectives and experience.

This thesis re-examines the territorial dispute between Japan and Russia, the so-called “Northern Territories” issue, through a reinterpretation of the role of the indigenous Ainu of Japan. An exploration of Ainu history and historiography reveals that the long-standing emphasis on Wajin-based legitimacy of rule and annexation of northern areas was replaced by historical amnesia concerning the role and status of the Ainu. Discussion focuses on an interpretation of Ainu understandings of local, regional/national and international historical events. This approach underscores the importance of de-nationalising History by integrating the important perspectives of Indigeneity. It asserts, further, that the understanding of these events and processes require a broader disciplinary prism than that provided by the study of history. The preponderance of nation-based studies, and not only in the field of History, has seriously inhibited the analysis of historical phenomena involving Indigenous peoples, in this case the Ainu. The study of the Northern Territories issue offers, then, both a new perspective on the history of this important dispute and an illustration of the importance of broadening traditional academic studies in disciplines such as History, Anthropology, Ecology, Political Science, International Relations and Law to incorporate Indigenous perspectives and experience.

The West India Royal Commission of 1897 advanced a number of recommendations intended to lift the West Indies out of their depressed condition and to shape their future economic development. This thesis examines the efforts made to implement those recommendations and the extent to which they influenced economic progress in the colonies of Barbadoes, British Guiana, The Windward and Leeward Islands. Particular attention is directed towards the recommendation that the labouring populations be encouraged to settle on the land as small proprietors. This proposal provided for the welfare of the largely Negro populations of the colonies, but it also threatened to upset the plantation dominated nature of the agriculture economy. The Royal Commission believed that peasant land ownership could be extended only through the introduction of government schemes of land settlement. The considerations which underlay the success or failure of such schemes and of peasant proproetorship in gneral constitute the central theme of the thesis. Other aspects of the economu which are examined affected planter and peasant alike. Freight connections, choice of crops, methods of cultivation, availability of markets, and access to expert advice were considerations which determined the success of both plantation and peasant proprietary. The Colonial Office role in the development of these colonies was limited and for the most part initiative rested with the colonies themselves. Questions of crop selection, or of the location for a settlement scheme, could only be decided by local experts. Furthermore, Joseph Chamberlain, the most influential Colonial Secretary of the period, as far as the West Indies were concerned, clearly believed in delegating responsibility to the local official. Nevertheless, when appropriate, the Colonial Office did play an active part. Its influence over shipping contracts was considerable, whilst the survival of the valuable Imperial Department of Agriculture, established in consequence of a recommendation of the Royal Commission, was entirely due to Colonial Office determination in the face of Treasury resistance.

This dissertation investigates the ways visitors to Haida Gwaii (sometimes called the Queen Charlotte Islands) have written about the islands. I argue that accounts by visitors to Haida Gwaii fashion the object that they seek to represent. In short, visitors' stories do not unproblematically reflect the islands but determine how Haida Gwaii is perceived. These perceptions in turn affect the actions of visitors, residents and governments. I contribute to that representational process, striving to show the material consequences of language and the ways discourses shape Haida Gwaii. The dissertation consists of three sections. "Early visitors" focuses on the last quarter of the eighteenth century, studying the earliest documented visits by Euro-American mariners and fur traders. "Modern visitors" concentrates on the last quarter of the nineteenth century and the beginning of the twentieth century, when some visitors were busy imposing colonial forms of government and social organization, while others were resisting these projects. "Recent visitors" concentrates on the final quarter of the twentieth century, examining the campaign to save a portion of the archipelago from clearcutting and efforts to develop alternatives to resource-extractive economic practices. By examining three case studies for each period, I argue that the ways visitors imagine the islands have been transformed in each of these periods.

Thesis (Ph. D.)--University of Hawaii at Manoa, 1995. / Includes bibliographical references (leaves 314-339). / Microfiche. / xi, 339 leaves, bound maps 29 cm